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A comparative study of Neogymnomyces virgineus,a new keratinolytic species from dung,and its relationships with the Onygenales

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Fungal Diversity 2012年第1期52卷 13-34页

作者： Francesco Doveri Susanna Pecchia Mariarosaria Vergara Sabrina Sarrocco giovanni vannacci Department of Tree Science Entomology and Plant Pathology“G.Scaramuzzi”Section Plant PathologyUniversity of Pisavia del Borghetto 8056124 PisaItaly

Isolations of onygenalean fungi were made recently from different dung samples from Italy.A striking snowwhite species with gymnothecial ascomata,developed in damp chamber on dormouse dung collected in a cave,was subjected to keratinolytic tests and morphological,cultural,and phylogenetic *** keratinolytic ability of this species,associated with a Chrysosporium anamorph and a sexual state of appendiculate reticuloperidia and oblate ascospores,allows it to be accomodated in *** ascomata,blunt or subcapitate peridial appendages,pitted ascospores,and tuberculate conidia suggest it to be a new Neogymnomyces,and this was confirmed by parsimony analyses of LSU and ITS nrDNA *** recent phylogenetic analyses,the morphological and physiological features of order Onygenales and its families are re-examined and *** the introduction of a new species,Neogymnomyces is reviewed and compared with all other genera in *** Chrysosporium imperfect state of Neogymnomyces virgineus is described and compared to the anamorph of *** is also compared to the atypical Chrysosporium merdarium and to several other Chrysosporium species with echinulate to verrucose-tuberculate conidia,isolated from guano,dung,and nitrogen-rich soils in *** onygenalean fungi isolated from any kind of dung are discussed and their facultative coprophily ascribed to variable faecal contents of keratin or other degradable substances.A key to the families and genera of the Onygenales is provided.

关键词： Chrysosporium Coprophily Key Keratinolysis/keratinophily New Neogymnomyces Onygenalean fungi Phylogeny

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Sequence analysis of rDNA intergenic spacer region (IGS) as a tool for phylogenetic studies in Trichoderma spp.

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浙江大学学报(农业与生命科学版) 2004年第4期30卷 460-461页

作者： Mercatelli Elisabetta Pecchia Susanna Ciliegi Sandro vannacci giovanni Dipartimento di Coltivazione e Difesa delle Specie Legnose "G. Scaramuzzi" Sez. Patologia Vegetale Università di Pisa Via del Borghetto 80 I-56124 Pisa Italy

Different from ribosomal genes, which contain highly conserved sequences that are detected in all organisms, the intergenic spacer of rDNA (IGS) appears to be the most rapidly-evolving spacer region. For this reason we tested this region for phylogenetic studies. This report focuses on the study of IGS sequences of isolates belonging to Trichoderma section (T. viride, T. koningii, T. hamatum, T. erinaceus, T. asperellum) and Pachybasium section (T. harzianum, T. crassum, T. fasciculatum, T. oblongisporum, T. virens). Using the primer pair 28STD and CNS1, the Fast Start Taq DNA Polymerase (Roche), and a three temperature PCR protocol, products ranging from ca 1900 to 2400 bp were obtained from all tested isolates. The PCR product of 16 Trichoderma spp. isolates was cloned into a pGEM-TEeasy Vector (Promega) and sequenced. Based on a BLAST search we can conclude that the PCR product represents the whole IGS region. Multiple alignments of IGS sequences revealed two portions with different homology level. Portion A (ca 1660 bp) is the portion that contains 3’ end of 28S gene and is the more variable, while portion B (ca 830 bp), that contains the 3’ end of IGS region and the 5’ end of 18S gene, is the less variable. Comparing all sequences in region A 705 identical pairs occur out of 1704 total nucleotides (41.4%), while in region B identical pairs were 723 out of 832 total nucleotides (86.9%) . Sequence comparison of the two regions at intraspecific level (where it was possible) showed higher variability in region A (0.17%-6.8%) than in region B (0.0%-1.0%) . At interspecific level, performing all possible comparisons, the variability of region A (19.5%-52.7%) and B (0.8%- 16.9%). were significantly higher. Comparing sequences of species belonging to Trichoderma section variability of the two regions appears reduced if compared with that obtained from comparisons of species belonging to Pachybasium section. On the basis of sequence alignment, phylogenetic trees were o

关键词： IGS rDNA sequences Trichoderma spp. phylogeny

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