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Evaluation of Endectocides and Conventional Acaricides in the Control of the <i>Boophilus microplus</i>on Field-Kept Dairy Cattle in the State of Pernambuco-Brazil

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Open Journal of Veterinary Medicine 2012年第3期2卷 124-128页

作者： vania lucia assis santana Maria Aparecida Da Gloria Faustino Erika Korinfsky Vanderley Marilene Maria Lima Leucio Camara Alves Academic Unit of Serra Talhada Federal Rural University of Pernambuco Serra Talhada Brazil Department of Veterinary Medicine Federal Rural University of Pernambuco Recife Brazil Ministry of Agriculture Livestock and Food Supply National Laboratory of Animal and Plant Rua Dom Manoel de Medeiros Recife Brazil

The aim of the present study was to evaluate different acaricide treatments for the control of Boophilus microplus on field-kept dairy cattle in the state of Pernambuco, Brazil. The first phase of the experiment consisted of collecting the ingurgitated female Boophilus microplus directly from the hosts for attainment of larvae. After the attainment of larvae, the artificial infestation procedure began on the cattle, with larvae ages ranging from 7 to 14 days. On Day 0, animals were separated into 12 groups to receive the corresponding treatment: Abamectin;Ivermectin;Ivermectin LA;Amitraz;Amitraz + Ivermectin;Amitraz + Ivermectin LA;Amitraz + Abamectin;Association (Cypermethrin + Chlorpyrifos + Citronella);Association + Ivermectin;Association + Ivermectin LA;Association + Abamectin;and Control. Subsequent evaluations were made on post-treatment days +7, +14, +21, +28, +35, +42, +49, +56 and +63. Analyzing the post-treatment effectiveness per day, indices revealed considerable variation ranging from 0% to 96.63%. Such indices demonstrate the significant reduction in the number of ticks on the animals in some groups, especially in the Abamectin group. The analysis of the results demonstrates that the use of different avermectines can assist in the development of Boophilus microplus control programs, thereby reducing the number of acaricide applications and production costs related to ticks.

关键词： Boophilus microplus Tick Carrapaticide Efficiency Resistance

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Comparison of Two Protocols of Agar Gel Immunodiffusion(AGID)Used to Diagnose of Equine Infectious Anemia(EIA)

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Open Journal of Veterinary Medicine 2015年第7期5卷 169-174页

作者： Maria do Rosario Oliveira da Cunha Beltrao Cid Aristoteles de Siqueira Alencar Adriana Soares Leite Leonardo Tavares Freitas Julianne Cortizo Gonzalez vania lucia de assis santana Helio Cordeiro Manso-Filho Department of Veterinary Medicine Federal Rural University of PernambucoRecifeBrazil National Animal and Plant Laboratory Ministry of AgricultureLivestock and Food SupplyRecifeBrazil

The aim of the present study was to compare two protocols of Agar Gel Immunodiffusion (AGID) that can be used to diagnose Equine Infectious Anemia (EIA). Different volumes of reagents and support for agar gel were used, as recommended by the Ministry of Agriculture, Livestock and Food Supply (MAPA) and the World Organization for Animal Health (OIE). Equidae sera samples (n = 1000) were tested using a national commercial kit and different volumes (i.e. 25 μL—MAPA and 50 μL—OIE) of reagents and standard sera. Antigen was used as recommended by MAPA. The results were assessed by two operators, 48 hours after beginning the test. At the end of the experiment, a Kappa index of 1 was obtained by the operators, for the interpretation of the results and for the different volumes of reagents used. Similar results of agreement (Kappa index = 1) were obtained when using slides or Petri dishes as support for the agar gel. In both cases, an almost perfect agreement was achieved, suggesting that the use of different supports does not affect the results. These findings indicate that both protocols (MAPA and OIE) can be used to diagnose EIA.

关键词： Horses Equids EIA Diagnosis AGID Serology

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Evaluation of Endectocides and Conventional Acaricides in the Control of the Boophilus microplus on Field-Kept Dairy Cattle in the State of Pernambuco-Brazil

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Open Journal of Veterinary Medicine（兽医学） 2012年第3期

作者： vania lucia assis santana Maria Aparecida Da Gloria Faustino Erika Korinfsky Vanderley Marilene Maria Lima Leucio Camara Alves

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