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Floristic composition and edaphic relationships in ferruginous campo rupestre reference ecosystems

Journal of Mountain Science 2024年第3期21卷 719-733页

作者： G.Wilson FERNANDES Letícia RAMOS Dario C.PAIVA Jéssica CUNHA-BLUM vanessa matos gomes Daniel NEGREIROS Ecologia Evolutiva&Biodiversidade Departamento de GenéticaEcologia e Evolução/ICBUniversidade Federal de Minas Gerais31270-901Belo HorizonteMGBrazil Knowledge Center for Biodiversity 31270-901Belo HorizonteMGBrazil Department of Biological Sciences Institute of EnvironmentFlorida International UniversityMiamiFL 33199USA Programa de Pós Graduação em Biodiversidade e Uso dos Recursos Naturais Universidade Estadual de Montes ClarosMontes ClarosMGBrazil Laboratório de Estudo das Relações Solo-Planta Departamento de SolosUniversidade Federal Rural do Rio de JaneiroSeropédicaRGBrazil

Land use change and occupation have led to modifications in the environment causing loss of biodiversity and ecosystem services throughout the *** environments with high economic relevance,such as the ferruginous campo rupestre(rupestrian grassland known as Canga in Brazil),are even more susceptible to severe impacts due to their extreme habitat conditions and low *** determination of reference ecosystems based on the intrinsic characteristics of the ecosystem is essential for conservation as well as to the implementation of ecological *** proposed the reference ecosystem of the three main types of habitats of the ferruginous campo rupestre based on their floristic *** described the floristic composition of each habitat and evaluated the physicochemical properties of the soils and the relationship between plants and *** three habitats showed high diversity of plant species and many endemic species,such as Chamaecrista choriophylla,Cuphea pseudovaccinium,Lychnophora pinaster,and Vellozia *** distribution of vegetation was strongly related with the edaphic characteristics,with a set of species more adapted to high concentration of base saturation,fine sand,organic carbon,and iron,while another set of species succeeded in more acidic soils with higher S and silt *** provide support for the contention that the ferruginous campo rupestre is a mosaic of different habitats shaped by intrinsic local *** to recognize the floristic composition of each particular habitat can lead to inappropriate restoration,increased habitat homogenization and increased loss of biodiversity and ecosystem *** study also advances the knowledge base for building the reference ecosystem for the different types of ferruginous campo rupestre habitats,as well as a key database for highlighting those species contribute most to community assembly in this diverse and threatened tropical mountain ecosystem.

关键词： Canga Ecological restoration Extreme ecosystem Non-forest ecosystems Rupestrian grassland Taxon environment relationships

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Molecular evaluation of glutathione S transferase family genes in patients with sporadic colorectal cancer

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World Journal of Gastroenterology 2018年第39期24卷 4462-4471页

作者： Gabriela Helena Rodrigues-Fleming Glaucia Maria de Mendonca Fernandes Anelise Russo Patrícia matos Biselli-Chicote Joao gomes Netinho Erika Cristina Pavarino Eny Maria Goloni-Bertollo Genetics and Molecular Biology Research Unit-UPGEM Sao Jose do Rio Preto Medical SchoolFAMERPSao Jose do Rio PretoSP 15090-000Brazil Department of Surgery and Coloproctology Sao Joes do Rio Preto Medical SchoolFAMERPSao Joes do Rio PretoSP 15090-000Brazil

AIM To evaluate the association between polymorphismsin glutathione S transferases(GSTs) and the risk of sporadic colorectal cancer(SCRC), tumor progression and the survival of *** A case-control study of 970 individuals from the Brazilian population was conducted(232 individuals from the case group with colorectal cancer and 738 individuals from the control group without a history of cancer). PCR multiplex and PCR-RFLP techniques were used to genotype the GST polymorphisms. The tumors were categorized according to the TNM classification: tumor extension(T), affected lymph nodes(N), and presence of metastasis(M). Logistic regression, multiple logistic regression and survival analysis were used to analyze the data. The results are presented in terms of odds ratio(OR) and 95% confidence interval(CI). The level of significance was set at 5%(P ≤ 0.05).RESULTS Age equal to or over 62 years(OR = 8.79; 95%CI: 5.90-13.09, P < 0.01) and female gender(OR = 2.91; 95%CI: 1.74-4.37; P < 0.01) were associated with increased risk of SCRC. Analysis of the polymorphisms revealed an association between the GSTM1 polymorphisms and a risk of SCRC(OR = 1.45; 95%CI: 1.06-2.00; P = 0.02), as well as between GSTT1 and a reduced risk of the disease(OR = 0.65; 95%CI: 0.43-0.98; P = 0.04). An interaction between the presence of the wild-type allele of GSTP1 Ile105 Val polymorphism and tobacco consumption on risk of SCRC(OR = 2.33; 95%CI: 1.34-4.05; P = 0.05) was observed. There was an association between the GSTM1 null genotype and the presence of advanced tumors(OR = 2.33; 95%CI: 1.23-4.41; P = 0.009), as well as increased risk of SCRC in the presence of a combination of GSTT1 non-null/GSTM1 null genotypes(OR = 1.50; 95%CI: 1.03-2.19; P = 0.03) and GSTT1 non-null/GSTM1 null/GSTP1 Val~*(OR = 1.85; 95%CI: 1.01-3.36, P = 0.04). Combined GSTT1 non-null/GSTM1 null genotypes(OR = 2.40; 95%CI: 1.19-4.85; P = 0.01) and GSTT1 non-null/GSTM1 null/GSTP1 Val~*(OR = 2.92; 95%CI: 1.05-8.12; P

关键词： Colorectal neoplasms Smoking Alcohol Glutathione S transferase Genetic polymorphisms

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Microencapsulation with spray-chilling as an innovative strategy for probiotic low sodium requeijao cremoso processed cheese processing

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Food Bioscience 2022年第2期46卷 209-220页

作者： Ramon Silva Tatiana Colombo Pimentel Fernando Eust´aquio de matos Junior Erick Almeida Esmerino Monica Q.Freitas Carmen Silvia F´avaro-Trindade M´arcia Cristina Silva Adriano gomes Cruz Federal Institute of Education Scienceand Technology of Rio de Janeiro(IFRJ)Departamento of Food Rio de JaneiroBrazil Fluminense Federal University(UFF) Faculty of Veterinary Rio de JaneiroRio de JaneiroBrazil c Federal Institute of Paran´a ParanavaíParan´aBrazil Federal University of Juiz de Fora(UJFJ) Departement of NutritionBrazil University of S˜ao Paulo(UJFJ) Departement of Food EngineeringBrazil

The effect of sodium reduction and Lactobacillus acidophilus addition(free or microencapsulated)on the quality parameters of requeijao cremoso processed cheese was assessed for 90 days at 5℃.Three formulations were prepared:(CONT)regular salt content and curd fusion(90℃/2 min),(FREE)reduced salt content+free probiotic culture added after curd fusion(90℃/2 min),and(MICRO)reduced salt content+microencapsulated probiotic culture added before curd fusion(70℃/5 min).FREE and MICRO formulations showed increased monounsaturated and polyunsaturated fatty acid content,improved health indices(decreases in thrombogenic index and increases in desirable fatty acid index),and altered rheological properties(higher consistency index,apparent viscosity,elastic properties,and gel strength).In addition,the MICRO formulation showed higher texture sensory acceptance and probiotic counts higher than 6 log CFU/g during storage and simulated gastrointestinal ***,microencapsulation of probiotics with spray chilling represents an innovative solution in requeijao cremoso processed cheese.

关键词： Microencapsulation Probiotics Quality parameters Sodium reduction Processed cheese

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Carcass image segmentation using CNN-based methods

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Information Processing in Agriculture 2021年第4期8卷 560-572页

作者： Diogo Nunes Goncalves vanessa Aparecida de Moares Weber Julia Gindri Bragato Pistori Rodrigo da Costa gomes Anderson Vicoso de Araujo Marcelo Fontes Pereira Wesley Nunes Goncalves Hemerson Pistori Federal University of Mato Grosso do Sul Av.Costa e Silvas/nBairro UniversitarioCampo GrandeMS 79070-900Brazil INOVISAO Dom Bosco Catholic UniversityAvenida Tamandare6000CP 100Jardim SeminarioCampo GrandeMS 79117-900Brazil EMBRAPA Gado de Corte Av.Radio Maia830Vila PopularCampo GrandeMS 79106-550Brazil State University of Mato Grosso do Sul Av.Dom Antonio Barbosa4155Vila Santo AmaroCampo GrandeMS 79115-898Brazil

Carcass grading can be used as an important metric to determine meat ***,carcass grading is usually performed by a specialist,making it a subjective and errorprone *** increase the accuracy of such task,image-based systems have been proposed in the *** of the most important parts of an image-based system is the image segmentation,which aims to identify the regions of the carcass in the *** this paper,we propose the use of two recent image segmentation methods called Superpixel+CNN(Convolutional Neural Network)and *** evaluate both methods,we have also built a dataset of carcass images and their *** of approximately 96%of pixel accuracy show the robustness of the methods in carcass image *** novelty of this work is the proposal and comparison of recent deep learning methods that use CNN and superpixels in carcass *** this way,the methods can be used in carcass grading systems to increase the accuracy of the grading task.

关键词： Carcass grading Image segmentation Convolutional neural networks Superpixels

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CYP1A1,CYP2E1 and EPHX1 polymorphisms in sporadic colorectal neoplasms

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World Journal of Gastroenterology 2016年第45期22卷 9974-9983页

作者： Glaucia Maria M Fernandes Anelise Russo Marcela Alcantara Proenca Nathalia Fernanda Gazola Gabriela Helena Rodrigues Patrícia matos Biselli-Chicote Ana Elizabete Silva Joao gomes Netinho érika Cristina Pavarino Eny Maria Goloni-Bertollo Genetics and Molecular Biology Research Unit-UPGEM Department of Molecular Biology Sao José do Rio Preto Medical School FAMERP Departament of Biology UNESP-Sao Paulo State University Departament of Surgery and Coloproctology Service Sao José do Rio Preto Medical School FAMERP

AIM To investigate the contribution of polymorphisms in the CYP1A1, CYP2E1 and EPHX1 genes on sporadic colorectal cancer(SCRC) risk. METHODS Six hundred forty-one individuals(227 patients with SCRC and 400 controls) were enrolled in the study. The variables analyzed were age, gender, tobacco and alcohol consumption, and clinical and histopathological tumor parameters. The CYP1A1 *2A, CYP1A1 *2C CYP2E1 *5B and CYP2E1 *6 polymorphisms were analyzed by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP). The EPHX1 Tyr113 His, EPHX1 His139 Arg and CYP1A1 *2C polymorphisms were detected by real-time PCR. Chisquared test and binary logistic regression were used in the statistical analysis. Haplotype analysis was conducted using the Haploview program, version *** Age over 6 2 years was a risk factor for SCRC development(OR = 7.54, 95%CI: 4.94-11.50, P < 0.01). Male individuals were less susceptible to SCRC(OR = 0.55, 95%CI: 0.35-0.85, P < 0.01). The CYP2E1*5B polymorphism was associated with SCRC in the codominant(heterozygous genotype: OR = 2.66, 95%CI: 1.64-4.32, P < 0.01), dominant(OR = 2.82, 95%CI: 1.74-4.55, P < 0.01), overdominant(OR = 2.58, 95%CI: 1.59-4.19, P < 0.01), and log-additive models(OR = 2.84, 95%CI: 1.78-4.52, P < 0.01). The CYP2E1*6 polymorphism was associated with an increased SCRC risk in codominant(heterozygous genotype: OR = 2.81, 95%CI: 1.84-4.28, P < 0.01; homozygous polymorphic : OR = 7. 3 2, 9 5 % C I : 1.85-28.96, P < 0.01), dominant(OR = 2.97, 95%CI: 1.97-4.50, P < 0.01), recessive(OR = 5.26, 95%CI: 1.35-20.50, P = 0.016), overdominant(OR = 2.64, 95%CI: 1.74-4.01, P < 0.01), and log-additive models(OR = 2.78, 95%CI: 1.91-4.06, P < 0.01). The haplotype formed by the minor alleles of the CYP2E1*5B(C) and CYP2E1*6(A) polymorphisms was associated with SCRC(P = 0.002). However, the CYP1A1 *2A, CYP1A1 *2C, EPHX1 Tyr113 His and EPHX1 His139 Arg polymorphisms were not associated with *** In conclusion, the

关键词： Single-nucleotide polymorphisms Colorectal neoplasms Cytochrome P-450 CYP2E1 Cytochrome P-450 CYP1A1 Epoxide hydrolases 1

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Impacts of COVID-19 on children and adolescents:A systematic review analyzing its psychiatric effects

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World Journal of Psychiatry 2022年第11期12卷 1313-1322页

作者： Italo Wanderson de Moura Gabriel Danielly Gonçalves Sombra Lima Jeully Pereira Pires Nélio Barreto Vieira Aloisio Antonio gomes de matos Brasil Yara Talita gomes Pereira Erika Galvao de Oliveira HildsonLeandro de Menezes Nadia Nara Rolim Lima Alberto Olavo Advíncula Reis Ruan Neto Pereira Alves Uanderson Pereira da Silva Jucier Gonçalves Junior Modesto Leite Rolim-Neto School of Medicine Faculty of Medicine of Juazeiro do Norte-FMJ/IDOMEDJuazeiro do Norte 63040-360CearaBrazil School of Medicine Federal University of CaririBarbalha 63048-080CearaBrazil Mais Médicos Program Federal Government of Brazil-Ministry of HealthIguatu 63048-080CearaBrazil Julio Alves de Lira Hospital and Maternity Hospital HALHMBelo Jardim 55157-290PernambucoBrazil Nursing Department Doctor Leão Sampaio University Center-UNILEAOJuazeiro do Norte 63.041-140CearaBrazil Julio Alves de Lira Hospital and Maternity Hospital JALHMBelo Jardim 55157-290PernambucoBrazil Graduate Program(Post-Doctoral)in Neuro-Psychiatry UFPERecife 50670-901PernambucoBrazil Postgraduate Program(Master’s and Doctorate)in Public Health University of São Paulo-USPSão Paulo 01246-904São PauloBrazil School of Education USPSão Paulo 05508-040São PauloBrazil Internal Medicine-Division of Rheumathology at Hospital das Clínicas São Paulo UniversitySão Paulo 01246-903São PauloBrazil

OBJECTIVE To summarize the most relevant data from a systematic review on the impact of COVID-19 on children and adolescents,particularly analyzing its psychiatric *** This review was conducted according to the Preferred Reporting Items for Systematic Reviews and Meta-analyses(PRISMA)guidelines and included experimental studies(randomized-individually or pooled-and non-randomized controlled trials),observational studies with a group for internal comparison(cohort studies-prospective and retrospective-and case-control)and qualitative studies in the period from 2021 to *** The search identified 325 articles;we removed 125 *** selected 200 manuscripts,chosen by title and selected *** excluded 50 records after screening titles and abstracts,as they did not meet the inclusion *** retrieved 150 records selected for a full *** excluded 90 text articles and we selected 25 records for the(n)***:Due to the short period of data collection,from 2021 to 2022,there is a possibility of lack of relevant studies related to the mental health care of children and *** addition,there is the possibility of publication bias,such as only significant findings being *** The impact of COVID-19 on the mental health of children and adolescents is of great concern to child and youth *** such as fear,anxiety,panic,depression,sleep and appetite disorders,as well as impairment in social interactions caused by psychic stress,are punctual markers of pain and psychic suffering,which have increasing impacts on the mental health panorama of children and adolescents globally,particularly in vulnerable and socially at-risk populations.

关键词： Child psychiatry Adolescent psychiatry Mental health COVID-19 Kids Teens

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Factors Associated with Low Intake of Dietary Fiber in Inflammatory Bowel Disease Patients

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Health 2014年第11期6卷 1300-1309页

作者： vanessa Rosa Brito Oliveira Raquel Rocha Mirella Brasil Lopes Fernanda gomes Coqueiro Naiade Silveira Almeida Sandra Santos Valois Genoile Oliveira Santana Department of Sciences of Nutrition School of Nutrition Federal University of Bahia Salvador Brazil Gastroenterology Unit University Hospital Professor Edgard Santos Federal University of Bahia Salvador Brazil

Inflammatory bowel disease patients reduce their intake of foods rich in dietary fibers in an attempt to prevent recurrence of the disease, predisposing these patients to nutritional losses. The aim of this study was to evaluate the intake of dietary fiber and associated factors in a group of patients with inflammatory bowel disease. This was a cross-sectional study with 61 inflammatory bowel disease patients, and all participants were outpatients in Salvador, Bahia. Patients completed a semi-structured questionnaire that included questions about demographics, socioeconomic status and anthropometric and clinical information and a food frequency questionnaire to assess the intake of dietary fiber. The mean intake of dietary fiber was 28.2 ± 14.8 g/day for inflammatory bowel disease patients, 27.9 ± 10.1 g/day for ulcerative colitis (UC) patients and 28.9 ± 21.1 g/days for those with Crohn’s disease (CD) (p > 0.05). Most inflammatory bowel disease patients (52.5%) had intake below that recommended for dietary fiber. Inadequate consumption was present in 56.3% of CD patients and 43.8% of those with UC (p = 0.28). Men had lower fiber intake than women (p = 0.04). No significant associations between fiber intake and disease activity, location, presence of complications, gastrointestinal complaints, and nutrition counseling were found (p > 0.05). The low intake of dietary fiber was present in most patients, and the greatest inadequacy was found in males. Insufficient intake of dietary fiber appears to be linked to demographic features and not necessarily clinical characteristics relevant to inflammatory bowel disease.

关键词： Inflammatory Bowel Disease Dietary Fiber Eating

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子宫颈造影术作为子宫颈癌筛查乙酸目检的一种辅助检查

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世界核心医学期刊文摘（妇产科学分册） 2006年第9期2卷 59-60页

作者： Bomfim-Hyppólito S. Santana Franco E. gomes De matos Meneses Franco R. 李欢 R. N. Valente no. 3041 D. Torres CEP.: 60.125-071. Fotaleza -Ceara Brazil

Objective: Validate Digital Cervicography as adjunctive cervical cancer screening test to VIA and Cytology. Method: Women (1292) were submitted to both cytological and VIA tests to obtain a sample of 301 positive VIA cases (23.3% ) which have had the Digital Cervicography (DC) taken and evaluated. Just cases considered positive by DC (149/301 cases- 49.5% )- and/or positive Pap smear tests were referred to colposcopy and biopsy whenever indicated. Results: Cervical smear was positive in 5.4% , including LGSIL (4% ), HGSIL (1% ) and one case of invasive cancer (0.4% ), and 1.6% of ASCUS or *** identified 81 positive cases (74 LGSIL; 5 HGSIL; 2 cancers). The sensitivity of cytology was 14.8% , and the DC was 100% . Corresponding specificities were 95.4% and 69.1% , respectively. The NPV and PPV for DC were 100% and 54.4% . Conclusion: DC increases sensitivity, specificity and positive predictive value of VIA, becoming a valid adjunctive screening test for cervical cancer in low resource settings.

关键词：子宫颈癌辅助检查造影术宫颈癌筛查低度鳞状上皮内病变不典型鳞状细胞细胞学检查涂片阳性

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Challenges of the Global COVID-19 Pandemic and Invasive Fungal Pathogens in SARS-COV-2 Associations: A Dangerous Relationship

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Advances in Microbiology 2023年第1期13卷 1-23页

作者： Alessandra gomes Mariscal Beatriz Fernanda Siqueira Matias Cíntia Batista de Almeida Nadir Cléia Alves de Santana Simone matos de Campos Roseli de Souza Klaucia Rodrigues Vasconcelos Margareth Léa da Silva Maia Cristiane Coimbra de Paula Diniz Pereira Leite Júnior Faculdade EducareMT Cuiab&aacute Brazil Centro Universitá rio de Vá rzea Grande (UNIVAG) V&aacute rzea Grande Brazil Laborató rio Central de Saú de Pú blica do Estado de Mato Grosso (LACEN) Cuiab&aacute Brazil Instituto Ciê ncias Biomé dicas (ICB) Universidade de Sã o Paulo (USP) S&atilde o Paulo Brazil Laboratory Carlos Chagas Sabin Group Cuiab&aacute Brazil Instituto Adolfo Lutz Center of Parasitology and Micology Micology Nucleus S&atilde o Paulo Brazil Research Laboratory Medical School Universidade Federal de Mato Grosso (UFMT) Cuiab&aacute Brazil

Background: COVID-19 is a rapidly transmissible pneumonia-like illness caused by SARS-CoV-2 that out broke in China in 2019 and is currently circulating worldwide. In the current context of the SARS-CoV-2 pandemic, complications are observed in clinical settings for the treatment of severe COVID-19 disease in nosocomial settings, due to cases of fungal co-infections. Objective: To carry out a review on fungal infections associated with respiratory infections caused by COVID-19 (Sars-Cov-2) and their aggravation. Methodology: The purpose of this study is to inform the reader about the characteristics of SARS-CoV-2 and the main fungal species that are affecting patients undergoing treatment for severe COVID-19, provoking discussion of the importance of the proposed topic, in relation to co-infections by different fungal microorganisms. Result: 80 scientific studies were selected, resulting from patients with COVID-19 and most commonly observed in patients with a history of comorbidities such as diabetes mellitus, hypertension, kidney disease, severe liver disease, oncological diseases, obesity and with severe COVID-19. These data do not represent the total number of records of the disease in the world, but cases reported by researchers in their series, showing the overlapping of fungal co-infection through the compromised immune status due to the use of therapeutic drugs, dysregulation of the microbiota, age of patients and the severity itself of the severe inflammation caused by COVID-19. Conclusion: The immunosuppression caused by the infection of COVID-19 concomitant with its therapy through corticosteroid therapy and comorbidities of the patients made fungal infections more susceptible, and these interfere in the evolution of the case and in the treatment of COVID-19, being relevant to distinguish secondary infections to therapy and the best possible reconstitution of the clinical picture.

关键词： Fungal Coinfections COVID-19 Coronavirus Systematic Review

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Beneficial effects of losartan or telmisartan on the local hepatic renin-angiotensin system to counter obesity in an experimental model

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World Journal of Hepatology 2019年第4期11卷 359-369页

作者： Francielle Graus-Nunes Felipe de Oliveira Santos Thatiany de Souza Marinho Carolline Santos Miranda Sandra Barbosa-da-Silva vanessa Souza-Mello Laboratorio de Morfometria Metabolismo e Doencas Cardiovasculares Departamento de Anatomia Instituto de Biologia Roberto Alcantara Gomes

BACKGROUND Obesity has been associated with hepatic overexpression of the renin-angiotensin system(RAS).AIM To evaluate the action of two angiotensin II(ANGII) receptor blockers(losartan or telmisartan) on the modulation of local hepatic RAS and the resulting metabolic effects in a diet-induced obesity murine *** Twenty C57 BL/6 mice were randomly divided into two nutritional groups for 10 wk: control group(C, n = 5, 10% of energy as fat) or high-fat group(HF, n = 15,50% of energy as fat). After treatment started, the HF group was randomly divided into three groups: untreated HF group(n = 5), HF treated with losartan(HFL, n = 5) and HF treated with telmisartan(HFT, n = 5). The treatments lasted for 5 wk, and the dose was 10 mg/kg body *** HF diet induced body mass gain(+28%, P < 0.0001), insulin resistance(+69%, P =0.0079), high hepatic triacylglycerol(+127%, P = 0.0004), and overexpression of intrahepatic angiotensin-converting enzyme(ACE) 1/ANGII type 1 receptor(AT1 r)(+569.02% and +141.40%, respectively, P < 0.0001). The HFL and HFT groups showed higher ACE2/rMAS gene expression compared to the HF group(ACE2: +465.57%, P = 0.0002 for HFL and +345.17%, P = 0.0049 for HFT; rMAS:+711.39%, P < 0.0001 for HFL and +539.75%, P < 0.0001 for HFT), followed by reduced insulin/glucose ratio(-30% for HFL and-33% for HFT, P = 0.0181),hepatic triacylglycerol levels(-28%, P = 0.0381 for HFL; and-45%, P = 0.0010 for HFT, and Plin2 *** Modulation of the intrahepatic RAS, with favored involvement of the ACE2/rMAS axis over the ACE1/AT1 r axis after losartan or telmisartan treatments, caused hepatic and metabolic beneficial effects as demonstrated by reduced hepatic triacylglycerol levels coupled with reduced PLIN 2 expression and improved glycemic control.

关键词： Liver Telmisartan Losartan Obesity Angiotensin Ⅱ type 1 receptor C57BL/6 mouse

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