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Science China(Life Sciences) 2019年第4期62卷 526-534页

作者： vandeclécio lira da silva André Mauricio Ribeiro dos Santos Wilfredo Blanco Sandro José de Souza Bioinformatics Multidisciplinary Environment (BioME) Universidade Federal do Rio Grande do Norte (UFRN) Programa de Pós-Gradua??o em Genética e Biologia Molecular (PPGBM) Universidade Federal do Pará (UFPA) Department of Computer Science Universidade Estadual do Rio Grande do Norte (UERN) Brain Institute Universidade Federal do Rio Grande do Norte (UFRN)

The gain of transcription factor binding sites(TFBS) is believed to represent one of the major causes of biological *** we used strategies based on comparative genomics to identify 21,822 TFBS specific to the human lineage(TFBS-HS),when compared to chimpanzee and gorilla genomes. More than 40%(9,206) of these TFBS-HS are in the vicinity of 1,283 genes.A comparison of the expression pattern of these genes and the corresponding orthologs in chimpanzee and gorilla identified genes differentially expressed in human tissues. These genes show a more divergent expression pattern in the human testis and brain, suggesting a role for positive selection in the fixation of TFBS gains. Genes associated with TFBS-HS were enriched in gene ontology categories related to transcriptional regulation, signaling, differentiation/development and nervous ***, genes associated with TFBS-HS present a higher expression breadth when compared to genes in general. This biased distribution is due to a preferential gain of TFBS in genes with higher expression breadth rather than a shift in the expression pattern after the gain of TFBS.

关键词： TFBS transcript factor human evolution expression breadth

Microencapsulated phenolic-rich extract from juice processing grape pomace(Vitis *** Var):Effects on oxidative stability of raw and pre-cooked bovine burger

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Food Bioscience 2022年第6期50卷 2120-2129页

作者： Marcelo Edvan dos Santos silva Rodrigo lira de Oliveira Thamyres Cesar de Albuquerque Sousa Cristiani Viegas Brandão Grisi Valquíria Cardoso da silva Ferreira Tatiana Souza Porto Marta Suely Madruga Suzana Pedroza da silva Fábio Anderson Pereira da silva Post-Graduate Program in Food Science and Technology Department of Food EngineeringTechnology CentreFederal University of ParaibaJoão PessoaParaiba58051-900Brazil Northeast Biotechnology Network/RENORBIO Federal Rural University of Pernambuco (UFRPE)Dom Manoel de MedeirosDois Irmãos52171-900RecifePEBrazil Federal University of Pernambuco's Agreste (UFAPE) GaranhunsAv.Bom PastorBoa Vista55296-901 GaranhunsPEBrazil Post-Graduate Program in Agrifood Technology Federal University of ParaibaCampus Universitario IIIBananeirasPBBrazil

Grape processing generates residues rich in bioactive compounds with potential application in the meat ***,these substances are unstable and needs to be treated to allow their action during the processing and storage of meat *** this sense,a phenolic-rich extract from grape pomace was microencapsulated and evaluated for its potential to inhibit the oxidative process in raw and precooked beef burgers during cold *** hamburger formulations containing lyophilized extract(LEB),microencapsulated extract(MEB)and synthetic antioxidant sodium erythorbate(SEB)were prepared,pre-cooked,and evaluated for lipid and protein *** microencapsulation,with 95.0%efficiency,provided a material with a crystalline structure,rich in phenolics and with high antioxidant potential,showing 377.4±0.01,1768.1±0.07 and 1185.0±0.20μmol TEAC.100 g−1 for DPPH,ABTS and FRAP,*** values of malonaldehyde were found in LEB and MEB(2.9±0.3 and 1.4±0.5 mg ***−1,respectively)at the end of storage,with emphasis on for the hamburgers that received the microencapsulated *** behavior was observed for the analysis of carbonyl compounds,with values of 11.1±1.1,8.7±1.4 and 6.8±1.0 nM ***−1 in SEB,LEB and MEB,***,the freeze-dried and microencapsulated grape extracts showed superior results when compared to the synthetic antioxidant,increasing the oxidative stability of hamburgers,and avoiding significant color changes during the cold *** results indicate the high potential of the microencapsulated material to be used as a natural antioxidant in the meat industry.

关键词： Lipid oxidation Protein oxidation Meat products Natural antioxidant Encapsulation

xSPIDER_ML:Proposal of a Software Processes Enactment Language Compliant with SPEM 2.0

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Journal of Software Engineering and Applications 2012年第6期5卷 375-384页

作者： Carlos Portela Alexandre Vasconcelos Antonio silva Elder silva Mariano Gomes Maurício Ronny Wallace lira Sandro Oliveira Informatics Center Federal University of PernambucoRecifeBrazil Institute of Exact and Natural Sciences Federal University of ParáBelémBrazil

SPEM (Software Process Engineering Metamodel Specification) is the software processes modeling standard defined by OMG (Object Management Group). However, the process enactment support provided by this standard has many deficiencies. Therefore, the main objective of this paper is to propose a language for software process enactment based upon SPEM 2.0 concepts. First, we will present a critical analysis of the SPEM standard approach for enactment. Then, we will present xSPIDER_ML, an enactment language, and describe its structure, components and associated rules. In order to evaluate the proposed language, a case study is performed through a RUP (Rational Unified Process) process instantiation. The language presented in this paper is part of a support set of tools for flexible software process enactment. Additionally, this set of tools is in compliance with software process quality models.

关键词： Software Process Enactment Language SPEM RUP

A Comparative Analysis between BPMN and SPEM Modeling Standards in the Software Processes Context

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Journal of Software Engineering and Applications 2012年第5期5卷 330-339页

作者： Carlos Portela Alexandre Vasconcelos Antonio silva Ariane Sinimbú Elder silva Maurício Ronny Wallace lira Sandro Oliveira Centro de Informática Universidade Federal de PernambucoRecifeBrazil Instituto de Ciências Exatas e Naturais Universidade Federal do ParáBelémBrazil

The main objective of this paper is to analyze the representativeness of the SPEM (Software Process Engineering Metamodel Specification) and the BPMN (Business Process Modeling Notation) standards in the software processes modeling context. To perform this analysis, it was adopted a standard structure to define a software process based upon a process ontology. Then, the SPEM and BPMN standards notations and their semantically corresponding elements in the default process were identified. This mapping also includes components of the CMMI-DEV (Capability Maturity Model Integration for Development) and MR-MPS (Reference Model for Software Process Improvement) quality models. This was necessary to assist in the mapping evaluation through a case study which models the best practices of these quality models. Finally, we carried out an analysis of these standards through specific characteristics considered necessary to model and to represent software processes.

关键词： Software Processes Modeling SPEM BPMN Quality Models

Non-Polar Chemical Constituents of Atemoya and Evaluation of the Cytotoxic and Antimicrobial Activity

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Phyton-International Journal of Experimental Botany 2021年第3期90卷 921-931页

作者： Suzana Vieira Rabêlo Fernanda Granja da silva Oliveira Michele Miriam Calixto de lira Livia Macedo Dutra Adilson Sartoratto Marta Cristina Teixeira Duarte Maria Claudia dos Santos Luciano Maria Francilene Souza silva Cláudia doÓPessoa Manoel Odorico de Moraes Filho Emmanoel Vilaça Costa Jackson Roberto Guedes da silva Almeida Post-Graduate Program in Biotechnology(RENORBIO) Federal Rural University of PernambucoRecifePernambuco52171-900Brazil Post-Graduate Program in Biotechnology State University of Feira de SantanaFeira de SantanaBahia44036-900Brazil Center for Studies and Research of Medicinal Plants(NEPLAME) Federal University of San Francisco ValleyPetrolinaPernambuco56304-205Brazil Multidisciplinary Center of Chemical Biological and Agricultural Research(CPQBA)Campinas State UniversityCampinasSão Paulo13083-970Brazil National Laboratory of Experimental Oncology(LabNOE) Federal University of CearáFortalezaCeará60020-181Brazil Departament of Chemistry Federal University of AmazonasManausAmazonas69067-005Brazil

This study aimed to identify the chemical composition of essential oil from fruits(EOAF)and the hexanic crude extract from aerial parts(At-Hex)of atemoya(Annona cherimola x Annona squamosa),a hybrid belonging to the Annonaceae *** and antimicrobial activity was also *** was obtained by hydrodistillation using a Clevenger apparatus,and their composition was analyzed by gas chromatography-mass spectrometry(GC-MS)*** was tested against human tumor cell lines HCT-116(colon carcinoma),SF-295(glioblastoma),OVCAR-8(ovarian carcinoma)and HL60(leukemia)using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide(MTT)assay,while antimicrobial activity was conducted by bioauthography method against eleven microorganisms ***-four compounds were identified in the EOAF and twenty-nine in *** monoterpenes linalool(25.70%),α-pinene(10.38%),β-pinene(9.12%),transocimene(7.43%),and the sesquiterpene bicyclogermacrene(12.58%)were the major constituents of EOAF,whereas the sesquiterpene spathulenol(13.91%)was the main compound of ***-Hex showed a high cytotoxicity against SF-295(glioblastoma).These findings show an important chemotaxonomic contribution for the Annonaceae family,mainly for the Annona *** proved to be a promising source of substances with potential cytotoxic activity.

关键词： Annona GC-MS volatile compounds terpenoids biologic activities

Antihyperglycemic effect of Passiflora glandulosa cav. fruit rinds flour in streptozotocin-induced diabetic mice

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Asian Pacific Journal of Tropical Medicine 2018年第9期11卷 510-517页

作者： Erlandia Alves Magalhaes Queiroz Raquel Teixeira Terceiro Paim Sandra Machado lira Jose Ytalo Gomes da silva Carla Laine silva Lima Marcelo Oliveira Holanda Stephen Rathinaraj Benjamin Icaro Gusmao Pinto Vieira Maria Izabel Florindo Guedes Biotechnology and Molecular Biology Laboratory State University of CearaFortaleza Ceara Brazil Technological Development Park(PADETEC) Federal University of CearaPici Campus Fortaleza Ceara Brazil

Objective:To investigate the effect of administration of Passiflora glandulosa(***) fruit rinds flour on streptozotocin(STZ)-induced diabetic ***:The preliminary phytochemical screening and parameters such as centesimal composition and brine shrimp toxicity were *** in vivo study Swiss female mice were divided into four groups:NC-normal control;DC-diabetic control animals receiving saline;MET-diabetic animals receiving metformin(200 mg/kg);PFRF-diabetic animals receiving *** fruit rinds flour(200 mg/kg).All of them were treated for 28 *** was used in a single dose of 120 mg/kg to establish diabetic *** weight,water and food intake,fasting blood glucose were *** analysis of pancreas and liver were performed to evaluate STZ-induced tissue ***:Phytochemical screening showed the presence of flavanones and *** *** fruit rinds flour was non-toxic by the brine shrimp *** fruit rinds flour also reduced the loss of body weight and significantly decreased food intake in the diabetic ***,a significant reduction in blood glucose was observed for 15 d and this was maintained on 21 d and 28 d when compared with diabetic ***,the *** fruit rinds flour has a favourable effect on the histopathological changes of the pancreas in STZ induced ***:It is concluded that *** fruit rinds flour is a natural product that contains potent antioxidant compounds and presents good prospects for the improvement of diabetic mellitus by reducing serum glucose levels.

关键词： Diabetes mellitus Passiflora Blood glucose Histology Streptozotocin Mice

Maternal low protein diet induces persistent expression changes in metabolic genes in male rats

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World Journal of Diabetes 2020年第5期11卷 182-192页

作者： Allan de Oliveira lira JoséLuiz de Brito Alves Mariana Pinheiro Fernandes Diogo Vasconcelos david Filipe Santana João Henrique da Costa-silva Béatrice Morio Carol Góis Leandro Luciano Pirola Department of Nutrition Federal University of PernambucoVitoria de Santo AntãoPernambuco 55608680Brazil Department of Nutrition Federal University of ParaíbaJoao Pessoa 74556060Brazil Henrique da Costa-silva Luciano PirolaDepartment of Physical Education and Sport SciencesFederal University of PernambucoVitoria de Santo AntãoPernambuco 55608680Brazil Carmen(Cardiology Metabolism and Nutrition)LaboratoryINSERM U1060Lyon-1 UniversitySouth Lyon Medical FacultyPierre Benite 69310France

BACKGROUND Perinatal exposure to a poor nutritional environment predisposes the progeny to the development of metabolic disease at the adult age,both in experimental models and *** adaptive responses to maternal protein restriction have been reported in metabolic ***,the expression of glucose/fatty acid metabolism-related genes in adipose tissue and liver needs to be *** To evaluate the metabolic impact of perinatal malnutrition,we determined malnutrition-associated gene expression alterations in liver and adipose *** In the present study,we evaluated the alterations in gene expression of glycolytic/Krebs cycle genes(Pyruvate dehydrogenase kinase 4 and citrate synthase),adipogenic and lipolytic genes and leptin in the adipose tissue of offspring rats at 30 d and 90 d of age exposed to maternal isocaloric low protein(LP)diet throughout gestation and *** also evaluated,in the livers of the same animals,the same set of genes as well as the gene expression of the transcription factors peroxisome proliferator-activated receptor gamma coactivator 1,forkhead box protein O1 and hepatocyte nuclear factor 4 and of gluconeogenic *** In the adipose tissue,we observed a transitory(i.e.,at 30 d)downregulation of pyruvate dehydrogenase kinase 4,citrate synthase and carnitine palmitoyl transferase 1b gene *** transcriptional changes did not persist in adult LP rats(90 d),but we observed a tendency towards a decreased gene expression of leptin(P=0.052).The liver featured some gene expression alterations comparable to the adipose tissue,such as pyruvate dehydrogenase kinase 4 downregulation at 30 d and displayed other tissue-specific changes,including citrate synthase and fatty acid synthase upregulation,but pyruvate kinase downregulation at 30 d in the LP group and carnitine palmitoyl transferase 1b downregulation at 90 *** gene alterations,together with previously described changes in gene expression in

关键词： Metabolic adaptation Phenotype plasticity Liver Adipose tissue Metabolism Maternal protein undernutrition Rats

In vitro antibacterial effect of aqueous and ethanolic Moringa leaf extracts

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Asian Pacific Journal of Tropical Medicine 2011年第3期4卷 201-204页

作者： Jackson Rafael Oliveira Peixoto Giselle Cristina silva Renata Albuquerque Costa Joseires lira de Sousa Fontenelle Gustavo Hitzschky Fernandes Vieira Antonio Adauto Fonteles Filho Regine Helena silva dos Fernandes Vieira Sea Sciences Institute Federal University of CearaBrazil Fisheries Engineering Department Federal University of CearaBrazil Federal University of Ceara Brazil Biology Department State University of Acarau ValleyBrazil

Objective:To evaluate the antibacterial effect of aqueous and ethanolic moringa leaf extracts (Moringa oleifera) on the growth of gram-positive and negative ***:Paper disks were soaked with 100,200,300 and 400μL of extract at 20 g/180mL and 10 g/190 *** extracts were tested against Escherichia coli(ATCC25922),Staphylococcus aureus(ATCC25923),Vibrio parahaemolyticus,Enterococcus faecalis(ATCC29212),Pseudomonas aeruginosa(ATCC27853), Salmonella enteritidis(IH) and Aeromonas *** susceptibility tests were performed using the modified disk diffusion ***:The strains ***,*** and *** (IH) were resistant to all *** general,disks with 400μL extract were the most efficient against ***,***,*** and ***:The study indicates a promising potential for aqueous and ethanolic Moringa leaf extracts as alternative treatment of infections caused by the tested strains.

关键词： Moringa oleifera Activity antibacterial Gram positive and negative bacteria

Assessment of the Integrity and Retention of Chromium (VI) in Portland Cement Pastes

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Advances in Chemical Engineering and Science 2021年第1期11卷 1-17页

作者： Ferreira Raul Tadeu Lobato Fortaleza Dário Batista Amorim Kelly Aparecida da Encarnação lira Fernanda silva silva Marizete Helena Pereira Wagner Batista Santos Institute of Exact Sciences and Earth Federal University of Mato Grosso Barra do Gar&#231as/MT Brazil

Chromium is a heavy metal used for various applications such as in the manufacture of stainless steels, in chemicals for wood treatment, in tanneries, and in the refractory industry. The main problem associated with the use of chromium in these activities is the considerable volume of contaminated effluent that requires treatment, mainly due to its high mobility and toxicity in its hexavalent form. Immobilization methods arise as an efficient solution for the treatment of these wastes through converting them into inert and stable materials. The present paper aims to investigate Portland cement II’s paste properties with potassium dichromate addition in order to verify the chromium immobilization process. Analyses of moisture content, water absorption, axial compressive strength, and solubilization were done in samples at 7 and 28 days with 0%;0.5%;1.05;1.5%;2.0% and 2.5% potassium dichromate addition. The results showed the influence of this salt in cement hydration reactions, damaging the mechanical behavior and the water absorption capability of the material produced. In the solubilization test, all compositions had >99% efficiency of chromium immobilization in both ages of hydration, thus demonstrating the capability of a cementitious matrix to stabilize this metal.

关键词： Wastes Solidification-Stabilization Chromium Solubilization

Novel Protease from Aspergillus tamarii URM4634: Production and Characterization Using Inexpensive Agroindustrial Substrates by Solid-State Fermentation

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Advances in Enzyme Research 2016年第4期4卷 125-143页

作者： Osmar Soares da silva Rodrigo lira de Oliveira Cristina Maria Souza-Motta Ana Lúcia Figueiredo Porto Tatiana Souza Porto Northeast Biotechnology Network/RENORBIO Federal Rural University of Pernambuco (UFRPE) Recife Brazil Academic Unity of Garanhuns/UAG Federal Rural University of Pernambuco (UFRPE) Garanhuns Brazil Department Mycology Federal University of Pernambuco (UFPE) Recife Brazil Department of Morphology Physiology Animal/DMFA Federal Rural University of Pernambuco (UFRPE) Recife Brazil

This study reports the protease production from Aspergillus tamarii using agroindustrial residues as substrate for solid-state fermentation (SSF) and biochemical characterization. The highest protease production was obtained using wheat bran as substrate at 72 h fermentation with maximum proteolytic activity of 401.42 U/mL, collagenase of 243.0 U/mL and keratinase of 19.1 U/mL. The protease exhibited KM = 18.7 mg/mL and Vmax = 28.5 mg/mL/min. The optimal pH was 8.0 and stable in a wide pH range (5.0 - 11.0) during 24 h. The optimum temperature was 40°C. The proteolytic activity was inhibited by Cu2+ (33.98%) and Hg2+ (22.69%). The enzyme was also inhibited by PMSF (65.11%), indicating that is a Serine Protease. These properties suggest that alkaline protease from A. tamarii URM4634 is suitable for application in food industries and leather processing. Additionally, the present findings opened new vistas in the utilization of wheat bran and other effective agroindustrial wastes as substrates for SSF.

关键词： Protease Aspergillus tamarii Biochemical Characterization Solid-State Fermentation Agroindustrial Waste