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Screening for Spikelet Fertility and Validation of Heat Tolerance in a Large Rice Mutant Population

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Rice science 2019年第4期26卷 229-238页

作者： Sulaiman CHEABU Nat PANICHAWONG Prisana RATTANAMETTA Boonthong WASURI Poonpipope KASEMSAP Siwaret ARIKIT Apichart vanavichit Chanate MALUMPONG Department of Agronomy Faculty of Agriculture at Kamphaeng Saen Kasetsart University Nakhon Pathom 73140 Thailand Faculty of Science and Technology Rajabhat Nakhon Pathom University Nakhon Pathom 73000Thailand Department of Horticulture Faculty of Agriculture Kasetsart University Bangkok 10900Thailand Rice Science Center & Rice Gene Discovery Unit Kamphaeng Sean Campus Kasetsart University Nakhon Pathom 73140Thailand

A total of 10 000 M4 individuals in Jao Hom Nil (JHN) mutant population was treated with high temperature (40 ℃ to 45 ℃) during the day time (6 h) from the booting to the harvesting stages, and ambient temperature (33 ℃ to 35℃) was used as the control. The results of screening and yield trials found that the mutant line M9962 had a high spikelet fertility of 78% under heat stress. In addition, the other mutant lines, including M3181 and M7988, had a spikelet fertility of approximately 70%. However, the JHN wild type, Sin Lek, RD15 and RD33 had very low spikelet fertility of 34%, 14%, 9% and 4%, respectively. The lower spikelet fertility at an elevated temperature resulted in a dramatic decrease of filled grain and contributed to a loss in 100-grain weight. M9962 is a potential genetic stock for use in a heat tolerance breeding programme. In addition, spikelet fertility at high temperature was representative of heat tolerance and can be used as a screening trait for heat tolerance during the reproductive phase on a large scale.

关键词： high temperature rice spikelet fertility heat tolerance mutant

Evaluation of Agronomic Traits in Chromosome Segment Substitution Lines of KDML105 Containing Drought Tolerance QTL under Drought Stress

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Rice science 2012年第2期19卷 117-124页

作者： Vaiphot KANJOO Kanchana PUNYAWAEW Jonaliza L.SIANGLIW Suwat JEARAKONGMAN Apichart vanavichit Theerayut TOOJINDA Center for Agricultural Biotechnology Kasetsart UniversityKamphaeng Saen CampusNakhon Pathom 73140Thailand School of Agriculture and Natural Resources University of PhayaoPhayao 56000Thailand Rice Gene Discovery Unit National Center of Genetic Engineering and BiotechnologyKasetsart UniversityKamphaeng Saen CampusNakhon Pathom 73140Thailand Sakon Nakhon Rice Research Center Sakon Nakhon 47000Thailand Department of Agronomy Faculty of Agriculture at Kamphaeng SaenKasetsart UniversityKamphaeng Saen CampusNakhon Pathom 73140Thailand Rice Science Center Kasetsart UniversityKamphaeng Saen CampusNakhon Pathom 73140Thailand

Drought is a major abiotic constraint to rice production in rainfed lowland and insufficiently irrigated areas. The improvement of drought tolerant varieties is one of the strategies to reduce the negative effects of drought. Quantitative trait loci （QTLs） for primary and secondary traits related to drought tolerance （DT） on chromosomes 1, 3, 4, 8 and 9 that determined from double haploid lines derived from a cross between CT9993 and IR62266 were introgressed and dissected into small pieces in the genetic background of Khao Dawk Mall 105 （KDML105） to develop chromosome segment substitution line （CSSL） population. The CSSLs were evaluated at the reproductive stage for their agronomic performance and yield components under drought stress, and results were compared with irrigated condition. The flowering of CSSL lines was 6 to 7 d earlier than KDML105. The mean values of grain yields in the CSSLs were higher than KDML105 under drought and irrigated conditions. At irrigated condition, the grain yields of introgression lines carrying DT-QTLs from chromosomes 4 and 8 were higher than that of KDML105, whereas other traits showed little difference with KDML105. Analysis indicated that grain yield has positive correlation with plant height, tiller and panicle number per plant, and total grain weight per plant under drought stress while negatively correlated with days to flowering. As mentioned above, CSSLs showing good adaptation under drought stress can be used as genetic materials to improve drought tolerance in Thai rainfed lowland rice breeding program, and as materials to dissect genes underlying drought tolerance.

关键词： chromosome segment substitution lines drought tolerance quantitative trait loci drought stress Jasmine rice

Mining and validation of novel genotyping-bysequencing(GBS)-based simple sequence repeats(SSRs)and their application for the estimation of the genetic diversity and population structure of coconuts(Cocos nucifera L.)in Thailand

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Horticulture Research 2020年第1期7卷 717-732页

作者： Kanamon Riangwong Samart Wanchana Wanchana Aesomnuk Chatree Saensuk Phakchana Nubankoh Vinitchan Ruanjaichon Tippaya Kraithong Theerayut Toojinda Apichart vanavichit Siwaret Arikit Department of Biotechnology Faculty of Engineering and Industrial TechnologySilpakorn UniversitySanamchandra Palace CampusNakhon Pathom 73000Thailand National Center for Genetic Engineering and Biotechnology(BIOTEC) National Science and Technology Development Agency(NSTDA)Khlong LuangPathum Thani 12120Thailand Center for Agricultural Biotechnology Kasetsart UniversityKamphaeng Saen CampusNakhon Pathom 73140Thailand Rice Science Center Kasetsart UniversityKamphaeng Saen CampusNakhon Pathom 73140Thailand Chumphon Horticultural Research Center Department of AgricultureBangkok 10900Thailand Department of Agronomy Faculty of Agriculture at Kamphaeng SaenKasetsart UniversityKamphaeng Saen CampusNakhon Pathom 73140Thailand

Coconut(Cocos nucifera L.)is an important economic crop in tropical ***,the lack of a complete reference genome and the limitations of usable DNA markers hinder genomic studies and the molecular breeding of ***,we present the results of simple sequence repeat(SSR)mining from a high-throughput genotyping-bysequencing(GBS)study of a collection of 38 coconut accessions.A total of 22,748 SSRs with di-,tri-,tetra-,penta-and hexanucleotide repeats of five or more were identified,2451 of which were defined as polymorphic loci based on locus clustering in 38 coconut accessions,and 315 loci were suitable for the development of SSR *** hundred loci were selected,and primer pairs for each SSR locus were designed and validated in 40 coconut *** analysis of 74 polymorphic markers identified between 2 and 9 alleles per locus,with an average of 3.01 *** assessment of the genetic diversity and genetic relationships among the 40 coconut varieties based on the analysis of population structure,principal coordinate analysis(PCoA),and phylogenetic tree analysis using the 74 polymorphic SSR markers revealed three main groups of coconuts in *** identified SSR loci and SSR markers developed in this study will be useful for the study of coconut diversity and molecular *** SSR mining approach used in this study could be applied to other plant species with a complex genome regardless of the availability of reference genome.

关键词： breeding structure sequence

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稻米直链淀粉含量基因座位的分子标记定位

作物学报 2000年第6期26卷 777-782页

作者：黄祖六谭学林 Tragoonrung S vanavichit A 扬州大学农学院数量遗传研究室云南农业大学水稻研究所 DNAFingerpriningUnit KasetsartUniversityKamphangsaenNakornPathom73140Thailand

以直链淀粉含量 ( AC)中等的 CT9993和泰国优质的香软米 KDML10 5杂交产生的 152个重组近交系 ( RIL )为材料 ,构建了含 83个 RFLP、 69个 AFL P和 15个微卫星 ( SSL P)标记的分子标记连锁图 ,标记间平均距离为 12 .98c M。应用该连锁... 详细信息

以直链淀粉含量 ( AC)中等的 CT9993和泰国优质的香软米 KDML10 5杂交产生的 152个重组近交系 ( RIL )为材料 ,构建了含 83个 RFLP、 69个 AFL P和 15个微卫星 ( SSL P)标记的分子标记连锁图 ,标记间平均距离为 12 .98c M。应用该连锁图对控制稻米 AC的基因座位 ( QTL )进行了分析。结果表明 :稻米 AC主要受两个主效 QTL和 5个微效 QTL的共同控制 ,两个主效 QTL分别位于第 3和第6染色体上 ,它们分别与 R2 170和 R1962距离 10 .5c M和 3.6c M,其中 R1962与 wx基因紧密连锁。上述两个位点都以大于 8.0的 L OD值检测到。根据分析结果 ,初步推断这两个主效 QTL无互作。文中还讨论了在偏态群体中连锁作图和

关键词：稻米品质直锭淀粉含量 QTL 基因定位分子标记

Molecular Mapping QTLs for Gel Consistency in Rice

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Agricultural Sciences in China 2001年第1期 1-4页

作者： HUANG Zu-liu TAN Xue-lin XU Chen-wu vanavichit A. Laboratory of Quantitative Genetics Agricultural College Yangzhou University Yangzhou 225009 Rice Research Institute Yunnan Agricultural UniversityKunming 650201 Laboratory of Quantitative Genetics Agricultural College Yangzhou University Yangzhou 225009 Agricultural Engineering Center Kasetsart University Kamphangsaen Nakorn Pathom 73140 Thailand

A population of 152 recombinant inbred lines (RIIs) derived from a cross between CT9993, a japonica rice with hard consistency (GC) and Khoa Dawk Mali105 (KDML 105 ), a famous Thai rice(Oryza sativa L. ) with medium GC, was used for GC quantitative trait loci (QTLs) analysis. Three linkage maps were constructed with RFLP, AFLP and microsatellite (SSLP) markers. The first one consists of 83 RFLP markers with 17.53 cM of an average distance between markers. The second one consists of 83 RFLP and 69 AFLP markers with 13.22 cM average distance between markers and the third one consists of 83 RFLP, 69 AFLP and 15 SSLP markers, and has an average distance of 12.98 cM between markers. Based on these three maps, QTLs conferring GC were analyzed. The results show that GC is mainly controlled by two linked loci,which are located at the two flanks of RFLP marker R2170 on chromosome 3. The distance between the two linked QTLs is 25 cM and the two QTLs were determined by a LOD scores larger than 16.0. The number of minor QTIs controlling GC varies from 4- 9 in the three different linkage maps.

关键词： Rice quality Gel consistency (GC) Quantitative trait locus (QIL) Gene mapping Linkage map

Characterization of Myanmar Paw San Hmwe Accessions Using Functional Genetic Markers

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Rice science 2015年第2期22卷 53-64页

作者： Kyaw Swar Oo Alisa KONGJAIMUN Srisawat KHANTHONG Myint YI Tin Tin MYINT Siriporn KORINSAK Jonaliza Lanceras SIANGLIW Khin Myo MYINT Apichart vanavichit Chanate MALUMPONG Theerayut TOOJINDA Rice Division Department of Agricultural Research Rice Gene Discovery Unit National Centre for Genetic Engineering and BiotechnologyNational Science and Technology Development AgencyKasetsart University Agronomy Department Faculty of Agriculture at Kamphaeng SaenKasetsart University Center of Excellence on Agricultural Biotechnology Office of Higher Education CommissionMinistry of Education Plant Breeding Faculty of Agriculture at Kamphaeng SaenKasetsart University Plant Biotechnology Center Myanmar Agriculture Service Rice Science Center Kasetsart University

Paw San Hmwe （PSM） rice has been cultivated in many areas of Myanmar for a long time. Strong aroma, good taste and its elongation during cooking are the key characteristics of PSM rice. Thirty-one PSM accessions were genotypically characterized, and their physical grain and cooking quality traits were studied. We used specific gene markers associated with aroma, apparent amylose content （AAC） and alkali spreading value to determine the alleles carried by different PSM accessions. The results revealed that six PSM accessions （PSM10, PSM12, PSM13, PSM21, PSM22 and PSM30） had a 3-bp insertion in Os2AP gene. Gel consistency （GC） allele was predominant among the PSM accessions for gelatinization temperature （GT）, however, the phenotype observed was between low and intermediate GT because of the combination of the GC allele with the presence of low GT allele at heterozygous state from the other loci of the SSIla gene. Intermediate to high AAC was observed among the PSM accessions corresponding to the haplotype identified for the single nucleotide polymorphism G/T and the （Cm）n repeat in the Wx gene. The characterization and grouping data of PSM accessions posted benefits to Myanmar seed banks, and our results will help in maintaining the integrity of PSM rice variety.

关键词： Paw San Hmwe rice aroma apparent amylose content gel consistency gelatinizationtemperature alkali spreading value functional genetic marker grain quality haplotype analysis

QTL Mapping for Partial Resistance to Southern Corn Rust Using RILs of Tropical Sweet Corn

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American Journal of Plant Sciences 2013年第4期4卷 878-889页

作者： Kitti Wanlayaporn Jetsada Authrapun Apichat vanavichit Somvong Tragoonrung Department of Agonomy Faculty of Agriculture Kasetsart University Nakhon Pathom Thailand Genome Institute National Center for Genetic Engineering and Biotechnology Pathum Thani Thailand Interdisciplinary Graduate Program in Genetic Engineering Kasetsart University Bangkok Thailand

Southern corn rust is one of destructive diseases in maize caused by Puccinia polysora Undrew. A mapping population of tropical sweet corn recombinant inbred lines (RILs) derived from a cross between hA9104 and hA9035 inbred lines were set up to detect quantitative trait loci (QTLs) involved in partial resistance to southern corn rust. Eighty nine RILs were used to evaluate resistance levels using nine-point relative scale (1-9) at Sweet Seeds, Suwan Farm, Thailand include combined analysis. A genetic linkage map was constructed with 157 SSR markers, with a total length of 2123.1 cM, covering 10 chromosomes. Broad-sense heritability of individual location ranged from 0.76 and 0.82 and combined across locations was 0.87. Multiple QTL mapping (MQM) was applied for the identification of the QTLs. Fifteen QTLs were detected on chromosome 1, 2, 5, 6, 9 and 10 in both locations and combined across locations. QTLs on chromosome 1, 5 and 6 were contributed by alleles of resistant parent hA9104 while others were contributed by alleles from the susceptible parent, hA9035. Phenotypic variance of each QTL explained ranged from 6.1% to 41.8% with a total of 69.8% - 81.9%. QTL on chromosome 1, 6 and 10 were stable QTLs detected in both locations.

关键词： Sweet Corn Southern Corn Rust Partial Resistance Quantitative Trait Loci (QTLs) Combined across Locations

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水稻生育期的分子标记和数量性状位点分析

江苏农业学报 2000年第4期16卷 249-250页

作者：谭学林黄祖六 vanavichit A TRANGOONRUNG S 云南农业大学稻作研究所昆明650201 扬州大学农学院数量遗传研究室扬州225009 AgriculturalGeneticsandBiotechnologyCenter

水稻生育期(从播种到抽穗的天数)是影响水稻生长和品种布局的一个重要因素,它既受品种的遗传特性控制又受光、温、水、肥等生长条件的影响.为选育适用于一定生态环境和耕作制度的水稻品种,生育期是一个需要经常改良的性状.

关键词： RFLP 分子标记数量性状位点分析生育期水稻

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稻米胶稠度基因位点的标记和分析

中国农业科学 2000年第6期33卷 1-5页

作者：黄祖六,谭学林,徐辰武,vanavichit A. 扬州大学农学院数量遗传研究室!江苏扬州225009 云南农业大学水稻研究所扬州大学农学院数量遗传研究室!江苏扬州225009 Agriculturalengineeringcenter!KasetsartUniversity Kamphangsaen NakornPathom73140 Thailand

以硬胶稠度 (GC)的 CT9993和中等胶稠度的 KDML1 0 5水稻品系杂交产生的 1 52个重组近交系 (RIL)为材料 ,用 RFLP、AFLP和微卫星 (SSLP)标记构建了 3张分子标记连锁图 ,以此对控制稻米 GC的数量性状位点 (QTL)进行了分析。结果表明 ,稻... 详细信息

以硬胶稠度 (GC)的 CT9993和中等胶稠度的 KDML1 0 5水稻品系杂交产生的 1 52个重组近交系 (RIL)为材料 ,用 RFLP、AFLP和微卫星 (SSLP)标记构建了 3张分子标记连锁图 ,以此对控制稻米 GC的数量性状位点 (QTL)进行了分析。结果表明 ,稻米 GC主要受位于第 3染色体的两个连锁位点控制 ,它们分别位于 R2 1 70左、右两侧 8.8c M和 1 7c M处。上述两个位点都以大于 1 6.0的 LOD值检测到。控制 GC的微效 QTL的数目则因连锁图的不同而异 ,约为 4～9个

关键词：稻米品质胶稠度数量性状位点分子标记连锁图