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Effect of Combinations of Sucrose and Cryoprotectants on the Survival of Catfish Embryos （Pangasidae hypophthamus）

Journal of Agricultural Science and Technology(B) 2013年第12期3卷 887-892页

作者： Nguyen Thi Hong Nguyen Thi Nhung Nguyen Thi Uoc Nguyen Trung Thanh Nguyen Thi Hiep Nguyen van Hanh pham van Khanh Ha Thi Ngoc Nga Truong van Thuong Bui Xuan Nguyen Department of Embryo Biotechnology Institute of Biotechnology Vietnam Academy of Science and Technology 18-Hoang QuocViet Hanoi 8404 Vietnam Department of Environment and Pathology Research Institute for Aquaculture No. 2 Cai Be Tien Giang 8473 Vietnam Research Institute for Aquaculture No. 1 Dinh Bang Tu Son Bac Ninh 84241 Vietnam

Although cryopreservation of embryos has been used in most terrestrial animals, the application of this technique has not been succeeded for aquatic animals. In this study, the authors investigate the effect of different combinations of sucrose （SUC, C12H22OH） and cryoprotectants （CPAs） on the survival of the catfish embryos （Pangasidae hypophthalmus） at low temperatures （4, 0 and -20 ℃） for short-term storage. For this aim, embryos with somites and optic cups were exposed to different combinations of sucrose with methanol （SUC ＋ MeOH）, 1.2-propylene glycol （SUC ＋ PROH） and ethylene glycol （SUC ＋ EG） at four concentrations ratios：（1） 0.5 M SUC ＋ 0.5 M CPA; （2） 1 M SUC ＋ 0.5 M CPA; （3） 0.5 M SUC ＋ 1 M CPA; （4） 1 M SUC ＋ 1 M CPA for 40 min at 4, 0 and -20 ℃. Embryos kept in water at room temperature （RT）, 4, 0 and -20℃ were used as controls. The survival rate was expressed as a percentage of hatched embryos per total embryos treated. The results showed that the hatching rate declined significantly when embryos were stored in water at 0 ℃ and -20℃. For embryos at 0 ℃ storage, the highest survival rate （87.78%） was obtained with 1 M SUC ＋ 1 M MeOH combination while at -20 ℃, only embryos in the combined treatments of 0.5 M SUC ＋ 1 MEG and 0.5 M SUC ＋ 1 M PROH reached the hatching stage （40% and 83.33%, respectively）. In conclusion, the results showed that the catfish embryos are sensitive to sub-zero temperatures and the combined treatment of 0.5 M sucrose and 1 M propylene glycol can be used to protect catfish embryos from damages caused by low temperature （0 ℃ and -20 ℃）.

关键词： Catfish cryoprotectants embryos low temperature sucrose.

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Efficacy of He-Ne Laser in Combination with Topical Clindamycin in the Treatment of Acne Vulgaris (AV)

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Journal of Cosmetics, Dermatological Sciences and Applications 2020年第3期10卷 134-139页

作者： Bui Thi van Nguyen Ngoc Oanh Đặng Văn Em Huynh Thi Xuan Tam Nguyen Lan Anh pham Thi Minh Phuong Dermatology-Venereology and Allergy 108 Central Military Hospital Hanoi Vietnam Dermatology-Venereology Department Bach Mai Hospital Hanoi Vietnam pham Ngoc Thach University of Medicine Ho Chi Minh City Vietnam

Objective: To evaluate the efficacy of He-Ne laser in combination with topical clindamycin in the treatment of AV at 108 Military Central Hospital from Oct 2015 to Aug 2016. Subjects and Methods: 61 patients with AV were divided into 2 groups: Group 1: 31 patients were treated with He-Ne laser in combination with topical Clindamycin, Group 2: 30 patients were only treated with topical clindamycin. Laser HE-NE was applied 2 times/week for 6 weeks. Result: After 3 months of treatment Group 1: very good—51.6%, good—48.4%. Group 2: very good—30%, good—50% and moderate—20%. Conclusion: The combination of He-Ne laser with topical clindamycin shows more rapid clinical improvement compared to topical clindamycin alone in the treatment of mild and moderate AV.

关键词： Acne Vulgaris He-Ne Laser Clindamycin

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Seasonal Effects of Backscattering Intensity of ALOS-2 PALSAR-2 (L-Band) on Retrieval Forest Biomass in the Tropics

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Journal of Geoscience and Environment Protection 2020年第11期8卷 26-40页

作者： Luong viet Nguyen Hieu van Nguyen Lap Quoc Kieu Tu Trong To Thanh Kim Thi Phan Tuan Anh pham Chi Kim Tran Space Technology Institute Vietnam Academy of Science and Technology Hanoi Vietnam Thai Nguyen University of Sciences Thai Nguyen City Vietnam

This research has used the L-band radar from ALOS-2 PALSAR-2 and field work data for evaluation of seasonal effects of backscattering intensity on retrieval forest biomass in the tropics. The effects of seasonality and HH, and HV polarizations of the SAR data on the biomass were analyzed. The dry season HV polarization could explain 61% of the biomass in this study region. The dry season HV backscattering intensity was highly sensitive to the biomass compared to the rainy season backscattering intensity. The SAR data acquired in the rainy season with humid and wet canopies were not very sensitive to the in situ biomass. Strong dependence of the biomass estimates with season of SAR data acquisition confirmed that the choice of right season SAR data is very important for improving the satellite based estimates of the biomass. This research expects that the results obtained in this research will contribute to monitoring of the quantity and quality of forest biomass in vietnam and other tropical countries.

关键词： L-Band SAR ALOS-2 PALSAR-2 Backscattering Intensity Tropical Forest Biomass vietnam

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Stem cell-based therapy for human diseases

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Signal Transduction and Targeted Therapy 2022年第9期7卷 3242-3282页

作者： Duc M.Hoang Phuong T.pham Trung Q.Bach Anh T.L.Ngo Quyen T.Nguyen Trang T.K.Phan Giang H.Nguyen Phuong T.T.Le van T.Hoang Nicholas R.Forsyth Michael Heke Liem Thanh Nguyen Department of Research and Development Vinmec Research Institute of Stem Cell and Gene TechnologyVinmec Healthcare SystemHanoiVietnam Department of Cellular Therapy Vinmec High.Tech CenterVinmec Healthcare SsystemHanoiVietnam Institute for Science&Technology in Medicine Keele UniversityKeeleUK a Department of Biology Stanford UniversityStanfordCAUSA

Recent advancements in stem cell technology open a new door for patients suffering from diseases and disorders that have yet to be *** cell-based therapy,including human pluripotent stem cells(hPsCs)and multipotent mesenchymal stem cells(MSCs),has recently emerged as a key player in regenerative *** are defined as self-renewable cell types conferring the ability to differentiate into various cellular phenotypes of the human body,including three germ *** are multipotent progenitor cells possessing self-renewal ability(limited in vitro)and differentiation potential into mesenchymal lineages,according to the International Society for Cell and Gene Therapy(ISCT).This review provides an update on recent clinical applications using either hPSCs or MSCs derived from bone marrow(BM),adipose tissue(AT),or the umbilical cord(UC)for the treatment of human diseases,including neurological disorders,pulmonary dysfunctions,metabolic/endocrine-related diseases,reproductive disorders,skin burns,and cardiovascular ***,we discuss our own clinical trial experiences on targeted therapies using MsCs in a clinical setting,and we propose and discuss the MSC tissue origin concept and how MSC origin may contribute to the role of MSCs in downstream applications,with the ultimate objective of facilitating translational research in regenerative medicine into clinical *** mechanisms discussed here support the proposed hypothesis that BM-MSCs are potentially good candidates for brain and spinal cord injury treatment,AT-MSCs are potentially good candidates for reproductive disorder treatment and skin regeneration,and UC-MsCs are potentially good candidates for pulmonary disease and acute respiratory distress svndrometreatment.

关键词： diseases distress treatment

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Is convenience really king? Comparative evaluation of catastrophic costs due to tuberculosis in the public and private healthcare sectors of viet Nam: a longitudinal patient cost study

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贫困所致传染病(英文) 2024年第2期13卷 17-28页

作者： Nguyen Hoa Binh Quang Vo Luan Nguyen Forse Rachel Jeanette Wiemers Anja Maria Christine Huynh Huy Ba Dong Thuy Thi Thu Phan Yen Thi Hoang Creswell Jacob Dang Thi Minh Ha Nguyen Lan Huu Shedrawy Jad L?nnroth Knut Nguyen Tuan Dinh van Dinh Luong Annerstedt Kristi Sidney Codlin Andrew James National Lung Hospital Ha Noi Viet Nam Friends for International TB Relief Ha Noi Viet Nam Department of Global Public Health WHO Collaboration Centre On Tuberculosis and Social Medicine Karolinska Institute Stockholm Sweden Stop TB Partnership Geneva Switzerland pham Ngoc Thach Hospital Ho Chi Minh City Viet Nam

Background In viet Nam, tuberculosis (TB) represents a devastating life-event with an exorbitant price tag, partly due to lost income from daily directly observed therapy in public sector care. Thus, persons with TB may seek care in the private sector for its flexibility, convenience, and privacy. Our study aimed to measure income changes, costs and catastrophic cost incurrence among TB-affected households in the public and private *** Between October 2020 and March 2022, we conducted 110 longitudinal patient cost interviews, among 50 patients privately treated for TB and 60 TB patients treated by the National TB Program (NTP) in Ha Noi, Hai Phong and Ho Chi Minh City, viet Nam. Using a local adaptation of the WHO TB patient cost survey tool, participants were interviewed during the intensive phase, continuation phase and post-treatment. We compared income levels, direct and indirect treatment costs, catastrophic costs using Wilcoxon rank-sum and chi-squared tests and associated risk factors between the two cohorts using multivariate *** The pre-treatment median monthly household income was significantly higher in the private sector versus NTP cohort (USD 868 vs USD 578;P=0.010). However, private sector treatment was also significantly costlier (USD 2075 vs USD 1313;P=0.005), driven by direct medical costs which were 4.6 times higher than costs reported by NTP participants (USD 754 vs USD 164;Pviet Nam face similarly high risk of catastrophic costs whether treated in the

关键词： Tuberculosis Patient cost Catastrophic cost Private sector Comparative analysis viet Nam

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越共十三大前后越南腐败治理的基本走向

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当代世界社会主义问题 2023年第2期 98-110,167页

作者：王贤 [越]范文德广西大学公共管理学院广西民族大学南宁530004 越共中央理论委员会越南社会科学院河内100803

在越共十三大之前,越南的重拳反腐取得了显著成效,但贪腐形势依然不容乐观。为此,加强腐败治理依旧是越共十三大的重要议题之一。着眼未来,越共将努力推动“不会贪”的预防机制、“不敢贪”的惩治机制与“不必贪”的保障机制构建;坚持... 详细信息

在越共十三大之前,越南的重拳反腐取得了显著成效,但贪腐形势依然不容乐观。为此,加强腐败治理依旧是越共十三大的重要议题之一。着眼未来,越共将努力推动“不会贪”的预防机制、“不敢贪”的惩治机制与“不必贪”的保障机制构建;坚持“反腐无禁区、无特权、无例外、无受任何个人和组织影响”的“四无”原则,努力实现腐败治理“无法外之地”,突破“刑不上大夫”的特权限制;全力推进腐败治理全覆盖和保障反腐至高性,以提高党和政府公信力与促成反腐“压强”效应。此外,越南的腐败治理日益凸显民知、民议、民检、民监、民享的“五民”理念,采取的具体举措包括:强化信息公开机制,赋予人民更多参议权益和途径,积极构筑多维反腐防线,兑现“人民检察”的政治承诺以及积极落实“有功人员奖励政策”等。

关键词：越共十三大治理现代化体制机制创新腐败治理

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Induction of Anthracnose Disease Resistance on Chili Fruit by Treatment of Oligochitosan—Nanosilica Hybrid Material

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Agricultural Sciences 2017年第10期8卷 1105-1113页

作者： pham Dinh Dzung Le Thanh Hung Le Si Ngoc Hoang Dac Hiet Bui van Le Nguyen Tien Thang Dang van Phu Nguyen Ngoc Duy Nguyen Quoc Hien Research and Development Center for Hi-Tech Agriculture Ho Chi Minh City Vietnam Graduate University of Science and Technology Vietnam Academy of Science and Technology Hanoi Vietnam University of Science Vietnam National University Ho Chi Minh City Vietnam Research and Development Center for Radiation Technology Vietnam Atomic Energy Institute Ho Chi Minh City Vietnam

Oligochitosan (OC) with molecular weight Mw of 5000 g/mol was prepared by gamma Co-60 ray irradiation of chitosan solution. Nanosilica (nSiO2) with the size of 10 - 30 nm was synthesized by calcination of acid treated rice husk. The mixture of 1% OC - 1% nSiO2 was prepared by dispersion of nSiO2 in OC solution. The morphology of nSiO2 in the mixture of OC-nSiO2 was measured from images of transmission electron microscopy (TEM). The effect of foliar application of the mixture of OC-nSiO2 on the induction of resistance against anthracnose disease caused by Colletotrichum gloeosporioides fungus on chili fruits was investigated. Results indicated that foliar application of OC-nSiO2 with the concentration of 60 mg/l - 60 mg/l was found to be as the optimal treatment that reduced the disease severity on chili fruits to 22.2% compared with 90.0% of the control. Thus, OC-nSiO2 hybrid material could be considered as an effective biotic elicitor to prevent anthracnose disease infection for chili fruits. Furthermore, the prepared OC-nSiO2 hybrid material can also be used as an environmentally friendly agrochemical product for sustainable development of agriculture.

关键词： Oligochitosan Nanosilica Chili Fruit Colletotrichum gloeosporioides Anthracnose

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Applied Biofloc Technology for Target Species in the Mekong Delta in vietnam： A Review

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Journal of Environmental Science and Engineering(B) 2017年第4期6卷 165-175页

作者： Nguyen van Hoa Ta van Phuong Tran Ngoc Hai Chau Tai Tao Le Quoc viet Nguyen Thi Hong van Huynh Thanh Toi Tran Huu Le Vo Nam Son pham Quoc Anh Duy College of Aquaculture and Fisheries (CAF) Cantho University Cantho 270000-910000 Vietnam College of Applied Biology Tay Do University Cantho 270000-910000 Vietnam

BFT （Biofloc Technology） has been currently applied in Cantho University to a number of targeted species from fresh-to marine and saline water species in the Mekong Delta, e.g. striped catfish （Pangasianodon hypophthalmus）, white leg shrimp （Litopenaeus vannamei）, tiger shrimp （Penaeus monodon） and Artemia vinhchau strain （Artemia franciscana） for both in the lab-scales or production scales. The best growth and survival rate of striped catfish was obtained in 6%o. For white leg shrimp, the results indicated：（1） C （Carbon） source e.g. rice-flour or molasses supplementary based on the feed provided to promote survival, growth and shrimp biomass harvested; （2） it was fed less than 20% as usual but showed similar to those in the control; （3） combination of rice-flour and molasses at a ratio of 70：30 by weight of C and N （Nitrogen） in a ration of 15：1 enhanced shrimp survival and growth; （4） in intensive culture, shrimp could be against the infection of disease and end up with higher survival, growth in earthen ponds. For tiger shrimp, a set up for larvae till post larvae at different ratios of C and N and C：N of 30 PLI5 displayed with better survival rate （49.73 ± 7.07%） and production （74,596 ± 10.608 PL/m3）. Artemiafranciscana was set up at C：N = 10：1 and salinities from 35, 60, 80 and 100 ppt. After two weeks, there were no significant difference among treatment and the control （without biofloc） in term of survival and growth. Moreover, total embryos per female was not significant different with the control and even the number of embryos as cysts tended to be higher. BFT displayed its advantages when applied on culture system of different targeted species and there is no doubt that it could help to sustain aquaculture and save environment in the Mekong Delta in near future.

关键词： Biofloc techology striped catfish white leg shrimp tiger shrimp Artemia vinhchau strain.

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大气边界层大涡模拟入口湍流生成方法研究

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工程力学 2020年第7期37卷 68-76页

作者：周桐闫渤文杨庆山 pham van Phuc 王京学北京交通大学土木建筑工程学院北京100044 结构风工程与城市风环境北京市重点实验室北京100044 重庆大学土木工程学院重庆400044 清水建设技术研究所日本东京135-8530

生成满足大气边界层风场特性的入口湍流是开展结构风效应大涡模拟的关键问题之一。该文的主要目的是验证并探讨两类主要的大气边界层大涡模拟入口湍流生成方法的合理性与可行性。采用CDRFG(Consistent Discretizing Random Flow Generat... 详细信息

生成满足大气边界层风场特性的入口湍流是开展结构风效应大涡模拟的关键问题之一。该文的主要目的是验证并探讨两类主要的大气边界层大涡模拟入口湍流生成方法的合理性与可行性。采用CDRFG(Consistent Discretizing Random Flow Generation)方法和被动模拟法生成大气边界层风场,从统计特性、流场结构和计算效率等方面进行对比分析,比较不同网格系统下的数值模拟结果,提出结构风效应大涡模拟的网格划分策略。结果表明:相比于CDRFG方法,被动模拟法生成的流场结构更加合理,但无法预先考虑脉动风场的空间相关性,且需要较高的计算成本和先验的流场信息。计算域的网格分辨率对于统计特性和流场结构的模拟精度具有重要影响,而目标区域的网格分辨率应依据控制工程结构风致响应的主要频带范围确定。

关键词：大涡模拟大气边界层风场入口湍流 CDRFG方法被动模拟法网格分辨率

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大气边界层大涡模拟入口湍流生成方法综述

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工程力学 2020年第5期37卷 15-25页

作者：周桐杨庆山闫渤文 pham van Phuc 王京学北京交通大学土木建筑工程学院北京100044 结构风工程与城市风环境北京市重点实验室北京100044 重庆大学土木工程学院重庆400044 清水建设技术研究所日本东京135-8530

随着计算资源的飞速发展以及数值模拟技术的不断进步,大涡模拟被越来越多地应用于结构风工程领域的研究。运用大涡模拟准确模拟结构风效应的关键问题之一是生成满足大气边界层风场特性的入口湍流条件。预前模拟法和人工合成法是目前主... 详细信息

随着计算资源的飞速发展以及数值模拟技术的不断进步,大涡模拟被越来越多地应用于结构风工程领域的研究。运用大涡模拟准确模拟结构风效应的关键问题之一是生成满足大气边界层风场特性的入口湍流条件。预前模拟法和人工合成法是目前主流的两类大涡模拟入口湍流生成方法。该文阐述了不同入口湍流生成方法的基本原理,并梳理其在结构风工程领域的发展。从结构风工程研究的角度出发,对比分析不同方法的特点及适用性。最后,针对当前大气边界层大涡模拟入口湍流生成方法存在的问题,提出了未来研究的展望。

关键词：大涡模拟入口湍流生成方法综述结构风工程预前模拟法人工合成法

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