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Tree sapling vitality and recovery following the unprecedented 2018 drought in central Europe

森林生态系统:英文版 2023年第5期10卷 597-606页

作者： Mirela Beloiu schwenke valeska schönlau Carl Beierkuhnlein Institute of Terrestrial Ecosystems ETH ZurichZurichSwitzerland Department of Biogeography University of BayreuthUniversitätsstraße 3095447BayreuthGermany GIB Geographical Institute Bayreuth University of Bayreuth95447BayreuthGermany BayCEER Bayreuth Center of Ecology and Environmental Research University of Bayreuth95448BayreuthGermany

Background:Ongoing climate change is anticipated to increase the frequency and intensity of drought events,thereby affecting forest recovery dynamics and elevating tree mortality.The drought of 2018,with its exceptional intensity and duration,had a significant adverse impact on tree species throughout Central Europe.However,our understanding of the resistance to and recovery of young trees from drought stress remains limited.Here,we examined the recovery patterns of native deciduous tree sapling species following the 2018 drought,and explored the impact of soil depth,understory vegetation,and litter cover on this recovery.Methods:A total of 1,149 saplings of seven deciduous tree species were monitored in the understory of old-growth forests in Northern Bavaria,Central Germany.The vitality of the saplings was recorded from 2018 to 2021 on 170 plots.Results:Fagus sylvatica was the most drought-resistant species,followed by Betula pendula,Acer pseudoplatanus,Quercus spp.,Corylus avellana,Carpinus betulus,and Sorbus aucuparia.Although the drought conditions persisted one year later,all species recovered significantly from the 2018 drought,albeit with a slight decrease in vitality by 2021.In 2018,the drought exhibited a more pronounced adverse effect on saplings in deciduous forests compared to mixed and coniferous forests.Conversely,sapling recovery in coniferous and mixed forests exceeded that observed in deciduous forests in 2019.The pivotal factors influencing sapling resilience to drought were forest types,soil depth,and understory vegetation,whereas litter and forest canopy cover had a negative impact.Conclusion:Long-term responses of tree species to drought can be best discerned through continuous health monitoring.These findings demonstrate the natural regeneration potential of deciduous species in the context of climate change.Selective tree species planting,soil management practices,and promoting understory diversity should be considered when implementing adaptive management strategies to enhance forest resi

关键词： Drought stress Temperate forest Deciduous trees Climate change Extreme events Soil depth

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Virtual reality interface devices in the reorganization of neural networks in the brain of patients with neurological diseases

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Neural Regeneration Research 2014年第8期9卷 888-896页

作者： valeska Gatica-Rojas Guillermo Méndez-Rebolledo Laboratory of Human Motor Control Faculty of Health SciencesUniversity of Talca Laboratory of Human Motor Control School of KinesiologyFaculty of Health SciencesUniversity of Talca

Two key characteristics of all virtual reality applications are interaction and immersion. Systemic interaction is achieved through a variety of multisensory channels （hearing, sight, touch, and smell）, permitting the user to interact with the virtual world in real time. Immersion is the degree to which a person can feel wrapped in the virtual world through a defined interface. Virtual real- ity interface devices such as the Nintendo~ Wii and its peripheral nunchuks-balance board, head mounted displays and joystick allow interaction and immersion in unreal environments created from computer software. Virtual environments are highly interactive, generating great activation of visual, vestibular and proprioceptive systems during the execution of a video game. In addi- tion, they are entertaining and safe for the user. Recently, incorporating therapeutic purposes in virtual reality interface devices has allowed them to be used for the rehabilitation of neurological patients, e.g., balance training in older adults and dynamic stability in healthy participants. The improvements observed in neurological diseases （chronic stroke and cerebral palsy） have been shown by changes in the reorganization of neural networks in patients＇ brain, along with better hand function and other skills, contributing to their quality of life. The data generated by such studies could substantially contribute to physical rehabilitation strategies.

关键词： nerve regeneration virtual reality virtual environment virtual reality exposuretherapy rehabilitation stroke postural balance cerebral palsy aged review nintendo wii balanceboard neural regeneration

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Hierarchical Metal-Organic Frameworks with Macroporosity:Synthesis, Achievements,and Challenges

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Nano-Micro Letters 2019年第3期11卷 281-313页

作者： Huan VDoan Harina Amer Hamzah Prasanth Karikkethu Prabhakaran Chiara Petrillo valeska PTing Department of Mechanical Engineering University of BristolBristol BS81TRUK Department of Oil Refining and Petrochemistry Hanoi University of Mining and GeologyDuc ThangBac Tu LiemHanoiVietnam

Introduction of multiple pore size regimes into metalorganic frameworks(MOFs)to form hierarchical porous structures can lead to improved performance of the material in various applications.In many cases,where interactions with bulky molecules are involved,enlarging the pore size of typically microporous MOF adsorbents or MOF catalysts is crucial for enhancing both mass transfer and molecular accessibility.In this review,we examine the range of synthetic strategies which have been reported thus far to prepare hierarchical MOFs or MOF composites with added macroporosity.These fabrication techniques can be either pre-or post-synthetic and include using hard or soft structural template agents,defect formation,routes involving supercritical CO2,and 3D printing.We also discuss potential applications and some of the challenges involved with current techniques,which must be addressed if any of these approaches are to be taken forward for industrial applications.

关键词： Metal-organic frameworks Hierarchical Macroporous Composites

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Differences in standing balance between patients with diplegic and hemiplegic cerebral palsy

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Neural Regeneration Research 2013年第26期8卷 2478-2483页

作者： valeska Gatica Rojas Guillermo Méndez Rebolledo Eduardo Guzman Muoz Natalia Ibarra Cortés Caterine Berrios Gaete Carlos Manterola Delgado Laboratory of Human Motor Control Faculty of Health Sciences University of Talca Laboratory of Human Motor Control School of Kinesiology Faculty of Health Sciences University of Talca school of Kinesiology University Santo Tomas Student school of Kinesiology Faculty of Health Sciences University of Talca Program in Medical Sciences University of the Frontera

Maintaining standing postural balance is important for walking and handling abilities in patients with cerebral palsy. This study included 23 patients with cerebral palsy （seven with spastic diplegia and 16 with spastic hemiplegia）, aged from 7 to 16 years of age. Standing posture balance measurements were performed using an AMTI model OR6-7 force platform with the eyes open and closed. Patients with diplegic cerebral palsy exhibited greater center of pressure displacement areas with the eyes open and greater center of pressure sway in the medial-lateral direction with the eyes open and closed compared with hemiplegic patients, Thus, diplegic patients exhibited weaker postural balance control ability and less standing stability compared with hemiplegic cerebral palsy patients.

关键词： neural regeneration cerebral palsy postural balance postural control center of pressure children diplegia hemiplegia grants-supported paper neuroregeneration

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Dioxins in the Food Chain: Contamination Fingerprint Analysis in Breeding Hens, Hatching Eggs and Broilers

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Journal of Environmental Protection 2014年第13期5卷 1323-1330页

作者： Miguel Oliveira Cardo Marta Castel-Branco valeska Andreozzi Fernando Almeida Bernardo Faculty of Veterinary Medicine University of Lisbon (FMV/UL) Lisbon Portugal Center of Statistics and Applications of University of Lisbon (CEAUL) Lisbon Portugal

While routine monitoring poultry meat was obtained from breeding hens, dioxins contaminations were detected in Portugal. Levels of 430.9 pg PCDD/F-WHO-TEQ/g1 were found, which are higher than the official limits legally allowed for this matrix (1.75 pg PCDD/F-WHO-TEQ/g). To identify the magnitude of the contaminations, 60 samples were collected from poultry farms and different matrices, namely: feed, water, wood shavings from the litters, muscle of the breeding hens, hatching eggs collected in the positive farm and muscle collected from broilers farms supplied by the positive breeding farm. The comparison of the dioxins congeners profiles showed that there was a coincidence of peaks of higher relative concentrations in the wood shavings, with the peaks of the highest relative concentration in the hatching eggs, especially the relative concentrations of the congeners 1,2,3,4,6,7,8-HpCDD and OCDD, which may be explained by the role of VLDLy in the delivery of triglycerides to the oocyte, where they will be used as the energy source for the developing embryo. The comparison of the dioxins congeners profiles of the breeding hens muscle with the poultry muscle, showed a coincidence of peaks of higher relative concentrations in the congeners 1,2,3,7,8-PeCDD, 1,2,3,6,7,8-HxCDD, 1,2,3,4,6,7,8-HpCDD and OCDD which may indicate a dechlorination pathway “in vivo”. Results allowed concluding that those wood shavings, improperly used as poultry litters, were certainly the source of contamination of the food chain.

关键词： Dioxin Fingerprint Food Chain Poultry

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高品质的薄膜包装

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流程工业 2019年第8期 38-39页

作者： valeska Haux Wolfertschwenden市Multivac公司

在生产药品包装物时,有许多不同的材料可供选用。但不是每一种塑料都适用于各种医药产品的包装。例如,包装材料的"可杀菌消毒性能是重要的选择标准之一。在标准的杀菌消毒工艺中.型料包装物必须能够在I2l℃的高温下经得起20min的考验,... 详细信息

在生产药品包装物时,有许多不同的材料可供选用。但不是每一种塑料都适用于各种医药产品的包装。例如,包装材料的"可杀菌消毒性能是重要的选择标准之一。在标准的杀菌消毒工艺中.型料包装物必须能够在I2l℃的高温下经得起20min的考验,还要保持其形状的稳定性不变并且不会有水蒸气渗透。聚丙烯材料在满足这些要求的基础上,还能满足其他更高的一些要求,因为PP聚丙烯材料的水蒸'I渗透性能明显低TPVC和PET材料。

关键词：薄膜包装聚丙烯材料品质包装材料 PET材料医药产品消毒性能消毒工艺

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H pylori (CagA) and Epstein-Barr virus infection in gastric carcinomas:Correlation with p53 mutation and c-Myc,Bcl-2 and Bax expression

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World Journal of Gastroenterology 2008年第6期14卷 884-891页

作者： valeska Portela Lima Marcos Antonio Pereira de Lima Angela Rosa André Márcia Valéria Pitombeira Ferreira Marcos Aurélio Pessoa Barros Sílvia Helena Barem Rabenhorst Departament of Pathology and Forensic Federal University of Ceará State Brazil Saint House of Mercy in Fortaleza Brazil

AIM: To investigate the interrelationship between H pylori and Epstein-Barr virus (EBV) infection in the gastric carcinogenesis having in focus the p53 mutation and the c-Myc, Bcl-2 and Bax expression. METHODS: seventy-one gastric carcinoma tissues were assessed by polymerase chain reaction (PCR) for H pylori and in situ hybridization for EBV. c-Myc, Bcl-2 and Bax expression were detected by immunohistochemistry and single-stranded conformational polymorphism (SSCP) for p53 mutation. RESULTS: The positivity rates for H pylori and EBV were 94.4% and 8.45%, respectively. The majority of the cases displayed only the H pylori presence. All EBV positive cases were also H pylori positive. None infectious agent was observed in 5.55% of the cases. The intestinal type tumor was more frequent in the co-infected and non-infected groups. The female predominated in the non-infected group showing statistical significance (70.4% vs 29.6%, P=0.039). The Bcl-2 was only detected in the group exclusively infected by H pylori. However, c-Myc and Bax were detected in the three groups but with a low frequency in the co-infected group. Mutation of p53 was present in all groups, with the highest frequencies in the H pylori positive groups. CONCLUSION: The frequency of H pylori infection in gastric carcinomas was high. The presented data indicated that gastric carcinogenesis has different pathways depending of the presence of the two investigated infectious agents, suggesting a possible involvement of H pylori with apoptotic process. The low expression of c-Myc and Bax in the EBV-positive groups suggests that EBV may inhibit the expression of these proteins. Nevertheless, p53 mutation shows to be a relevant alteration, independent of both infectious agents.

关键词：胃癌幽门螺杆菌 p53 Bax Bcl-2 细菌感染

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Green Liver Systems for Water Purification:Using the Phytoremediation Potential of Aquatic Macrophytes for the Removal of Different Cyanobacterial Toxins from Water

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American Journal of Plant Sciences 2015年第9期6卷 1607-1618页

作者： Stephan Pflugmacher Sandra Kühn Sang-Hyup Lee Jae-Woo Choi Seungyun Baik Kyu-Sang Kwon valeska Contardo-Jara Technische Universitat Berlin Institute of EcologyChair Ecological Impact Research and EcotoxicologyBerlinGermany Korea Institute of Science and Technology Centre for Water Resource Cycle ResearchSeoulRepublic of Korea Graduate school of Convergence Green Technology and Policy Korea UniversitySeoulRepublic of Korea Department of Energy and Environmental Engineering University of Science and Technology(UST)DaejeonRepublic of Korea KIST Europe Forschungsgesellschaft mbH Campus E71Environment and Bio GroupSaarbrückenGermany

The protection and reasonable use of freshwater is one of the main goals for our future, as water is most important for all organisms on earth including humans. Due to pollution, not only with xenobiotics, but also with nutrients, the status of our water bodies has changed drastically. Excess nutrient load induces eutrophication processes and, as a result, massive cyanobacterial blooms during the summer times. As cyanobacteria are known to produce several toxic secondary metabolites, the so-called cyanotoxins, exhibiting hepato-, neuro- and cell-toxicity, a potential risk is given, when using this water. There is an urgent need to have a water purification system, which is able to cope with these natural toxins. Using aquatic plants as a Green Liver, the Green Liver System?, was developed, able to remove these natural pollutants. To test the ability of the Green Liver System?, several cyanobacterial toxins including artificial and natural mixtures were tested in a small-scale laboratory system. The results showed that within 7 - 14 days a combination of different aquatic macrophytes was able to remove a given toxin amount (10 μg·L-1) by 100%. The phytoremediation technology behind the Green Liver Systems? uses the simple ability of submerged aquatic plants to uptake, detoxify and store the toxins, without formation and release of further metabolites to the surrounding water.

关键词： Green Liver System Phytoremediation Aquatic Macrophytes Water Purification Biotransformation Metabolism Cyanobacterial Toxins

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氨基酮戊酸光动力疗法抑制成纤维细胞生长因子10诱导的HaCaT细胞过度角化及增殖的机制研究

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中华皮肤科杂志 2015年第11期48卷 792-796页

作者：易飞 Maya valeska Gozali 张家安周炳荣骆丹张丽超王申刘娟吴红巾南京医科大学第一附属医院皮肤科 210029

目的探讨氨基酮戊酸光动力疗法（ALA-PDT）对成纤维细胞生长因子10（FGF-10）诱导的HaCaT细胞过度角化及增殖的作用及其机制。方法将培养的HaCaT细胞分为4组：空白对照组（无任何处理），FGF-10组（加入FGF-10孵育24 h），ALA-PDT组... 详细信息

目的探讨氨基酮戊酸光动力疗法（ALA-PDT）对成纤维细胞生长因子10（FGF-10）诱导的HaCaT细胞过度角化及增殖的作用及其机制。方法将培养的HaCaT细胞分为4组：空白对照组（无任何处理），FGF-10组（加入FGF-10孵育24 h），ALA-PDT组（ALA孵育24 h后红光照射），FGF-10 ＋ ALA-PDT组（FGF-10孵育24 h后，再加ALA孵育24 h，红光照射）。用CCK-8法检测各组细胞增殖活性，Western印迹法检测K1、K6、K16及角质形成细胞生长因子受体（KGFR）的含量，ELISA法检测各组细胞上清液中白细胞介素1α（IL-1α）蛋白表达，免疫荧光检测各组细胞KGFR和K6蛋白表达水平。统计学分析采用析因设计的方差分析和单因素方差分析。结果析因分析显示，ALA-PDT和FGF-10对HaCaT细胞增殖无交互作用（F交互 = 1.369，P = 0.276），FGF-10对HaCaT细胞增殖有促进作用（FFGF-10 = 20.853，P 〈 0.05），而ALA-PDT有抑制作用（FALA-PDT = 24.822，P 〈 0.05）。与空白对照组细胞增殖活性（A值为0.924 ± 0.024）比较，FGF-10组（1.233 ± 0.099）显著升高（P 〈 0.05），而ALA-PDT组（0.718 ± 0.107）显著降低（P 〈 0.05），FGF-10 ＋ ALA-PDT组（0.901 ± 0.014）较FGF-10组显著降低（P 〈 0.05）。Western印迹法显示，FGF-10可促进K1、K6、K16蛋白和KGFR的表达（均P 〈 0.05），而ALA-PDT抑制这些蛋白的表达（均P 〈 0.05），FGF-10 ＋ ALA-PDT组K1、K6、K16和KGFR的相对表达量与FGF-10组比较均显著降低（均P 〈 0.05）。ELISA法显示，FGF-10会增加HaCaT细胞IL-1α蛋白分泌（P 〈 0.05），但ALA-PDT对其没有影响（P = 0.467）。此外，免疫荧光检测显示，FGF-10增加HaCaT细胞K6和KGFR免疫荧光强度（P 〈 0.05），而ALA-PDT降低K6和KGFR免疫荧光强度（P 〈 0.05），FGF-10 ＋ ALA-PDT组K6和KGFR免疫荧光强度显著低于FGF-10组（P 〈 0.05）。结论 ALA-PDT能抑制FGF-10诱导的HaCaT细胞过度角化及增殖，其机制与下调K1、K6、K16、KGFR及IL-1α有关。

关键词：角蛋白细胞氨基酮戊酸成纤维细胞生长因子10 细胞增殖角蛋白1 角蛋白6 角蛋白16 白细胞介素1α 痤疮光动力疗法 HaCaT细胞

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ALA-PDT对UVB诱导的早衰成纤维细胞形态及增殖活性的影响

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中国皮肤性病学杂志 2015年第3期29卷 225-230页

作者：张丽超周炳荣骆丹马立文易飞王申 Maya valeska Gozali 吴红巾栗丹南京医科大学第一附属医院皮肤性病科江苏南京210029 南京大学医学院附属南京鼓楼医院皮肤科江苏南京210008

目的研究5-氨基酮戊酸光动力疗法（ALA-PDT）对中波紫外线诱导的提前衰老（UVB-SIPS）成纤维细胞形态及增殖活性的影响。方法将不同浓度5-氨基酮戊酸（5-aminolevulinicacid，ALA）孵育UVB-SIPS成纤维细胞，不同时间点检测细胞内原卟啉I... 详细信息

目的研究5-氨基酮戊酸光动力疗法（ALA-PDT）对中波紫外线诱导的提前衰老（UVB-SIPS）成纤维细胞形态及增殖活性的影响。方法将不同浓度5-氨基酮戊酸（5-aminolevulinicacid，ALA）孵育UVB-SIPS成纤维细胞，不同时间点检测细胞内原卟啉IX（ProtoporphyrinIX，PpⅨ）荧光强度；1mmol／LALA孵育正常及UVB．SIPS成纤维细胞2h，6h，采用635nm红光以不同照光剂量处理后，倒置显微镜观察细胞形态、CellCountingKit-8（CCK-8）法检测细胞活性。结果1．00mm01／LALA孵育6h内，UVB-SIPS成纤维细胞内PpIX荧光强度逐渐增强，6～24h荧光强度逐渐减弱。ALA-PDT可诱导UVB-SIPS成纤维细胞形态改变、增殖减缓，与照光剂量及ALA孵育时间呈量效依赖关系，使用抗氧化剂NAC预处理可以减轻上述改变。结论ALA-PDT对UVB-SIPS成纤维细胞的增殖和生长有一定的抑制作用，其机制可能与ALA．PDT对细胞的氧化损伤有关。

关键词： UVB诱导的早衰成纤维细胞 5-氨基酮戊酸光动力疗法细胞增殖

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