检索结果-南通市图书馆

Str.ctur.l and Functional Studies of the Mitochondr.al Cysteine Desulfur.se fr.m Ar.bidopsis thaliana

Molecular.Plant 2012年第5期5卷 1001-1010页

作者： valeria r. turowski Mar.a V. Busi Diego F. Gomez-Casati Centr. de Estudios Fotosinteticos y Bioquimicos (CEFOBI-CONICET) Universidad Nacional de Rosario Suipacha 531 2000 Rosario Argentina and UniversidadNacional de General San Martin (UNSAM) Av. Gral Paz 5445 San Martin Buenos Aires Argentina

AtNfsl is the Ar.bidopsis thaliana mitochondr.al homolog of the bacter.al cysteine desulfur.ses NifS and IscS, having an essential r.le in cellular.Fe-S cluster.assembly. Homology modeling of AtNfslm pr.dicts a high global similar.ty with E. coil IscS showing a full conser.ation of r.sidues involved in the catalytic site, wher.as the chlor.plastic AtNfs2 is mor. similar.to the Synechocystis sp. SufS. Pull-down assays showed that the r.combinant matur. for., AtNfslm, specifically binds to Ar.bidopsis fr.taxin （AtFH）. A hyster.tic behavior. with a lag phase of sever.l minutes, was obser.ed and hyster.tic par.meter. wer. affected by pr.-incubation with AtFH. Mor.over. AtFH modulates AtNfslm kinetics, incr.asing Vmax and decr.asing the S0.5 value for.cysteine. r.sults suggest that AtFH plays an impor.ant r.le in the ear.y steps of Fe-S cluster.for.ation by r.gulating AtNfsl activity in plant mitochondr.a.

关键词： cysteine desulfur.se Fe-S biogenesis mitochondr.a Ar.bidopsis fr.taxin.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effect of Mitochondr.al Dysfunction on Car.on Metabolism and Gene Expr.ssion in Flower.Tissues of Ar.bidopsis thaliana

引用

Molecular.Plant 2011年第1期4卷 127-143页

作者： Mar.a V. Busi Mar.a E. Gomez-Lobato Sebastian P. r.us valeria r. turowski Paula Casati Eduar.o J. Zabaleta Diego F. Gomez-Casati Alejandr. Ar.ya Instituto de Investigaciones Biotecnologicas-lnstituto Tecnologico de ChascomOs (IIB-INTECH) CONICET/UNSAM Chascomus Argentina Centr. de Estudios Fotosinteticos y Bioquimicos (CEFOBI) Universidad Nacional de Rosario Argentina Instituto de Investigaciones Biologicas liB CONICET-Universidad Nacional de Mar del Plata Argentina Micr.biologie Cellulair. et Moleculair. et Pathogenicite UMR 5234 Centre National de la Recherche Scientifique and Universite Victor Segalen-Bordeaux 2 Bordeaux France

We char.cter.zed the tr.nscr.ptomic r.sponse of tr.nsgenic plants car.ying a mitochondr.al dysfunction induced by the expr.ssion of the unedited for. of the ATP synthase subunit 9. The u-ATP9 tr.nsgene dr.ven by A9 and APETALA3 pr.moter. induce mitochondr.al dysfunction r.vealed by a decr.ase in both oxygen uptake and adenine nucleo- tides （ATP, ADP） levels without changes in the ATIP/ADP r.tio. Fur.her.or., we measur.d an incr.ase in r.S accumulation and a decr.ase in glutathione and ascor.ate levels with a concomitant oxidative str.ss r.sponse. The tr.nscr.ptome analysis of young Ar.bidopsis flower., validated by qr.-PCr.and enzymatic or.functional tests, showed dr.matic changes in u-ATP9 plants. Both lines display a modification in the expr.ssion of var.ous genes involved in car.on, lipid, and cell wall metabolism, suggesting that an impor.ant metabolic r.adjustment occur. in plants with a mitochondr.al dysfunction. Inter.stingly, tr.nscr.pt levels involved in mitochondr.al r.spir.tion, pr.tein synthesis, and degr.dation ar. affected. Mor.- over. the levels of sever.l mr.As encoding for.tr.nscr.ption factor. and DNA binding pr.teins wer. also changed. Some of them ar. involved in str.ss and hor.one r.sponses, suggesting that sever.l signaling pathways over.ap. Indeed, the tr.n- scr.ptome data r.vealed that the mitochondr.al dysfunction dr.matically alter. the expr.ssion of genes involved in signaling pathways, including those r.lated to ethylene, absicic acid, and auxin signal tr.nsduction. Our.data suggest that the mitochondr.al dysfunction model used in this r.por. may be useful to uncover.the r.tr.gr.de signaling mechanism between the nucleus and mitochondr.a in plant cells.

关键词： Electr.n tr.nspor. tr.nscr.ptome analysis Ar.bidopsis.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：