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Advances in Infectious Diseases 2012年第4期2卷 135-142页

作者： Thiago Azevedo Feitosa Ferro Francyelle Costa Moraes Andreia Meneses da Silva Claude Porcy Leandro Amorim Soares Cristina Andrade monteiro Nyla Thyara Melo Lobao Francisco Amazonas Assis de Mello valério monteiro-neto Patrícia de Maria Silva Figueiredo Research Group of Endemic and Parasitic Diseases CEUMA UniversitySao LuisBrazil Federal Institute of Maranhao Sao LuisBrazil Health and Biological Sciences Center Federal University of MaranhaoSao LuísBrazil

Enteroaggregative (EAEC) and atypical enteropathogenic (EPEC) Escherichia coli are important bacterial etiologic agents causing diarrhea among children. The aim of the present study was to examine the impact of virulence factors predisposes to diarrhea. In this study some virulence properties were examined on 11 EAEC and 8 EPEC strains identified by Polymerase Chain Reaction (PCR), isolated from stool samples of children were analyzed genotypically and phenoltypically for the prevalence of virulence factors. The most frequently detected factor was resistance to serum (94%), followed by curli fimbriae (78%), biofilm production (73%), and gene coding for Extended-Spectrum Beta-Lactamase (ESBL) (68%). EPEC isolates showed at least three of the evaluated properties, while EAEC isolates showed at least two. The prevalence of these virulence factors between the two strains showed no statistical difference. This study showed the heterogeneity of the virulence profile of the isolates of EAEC and atypical EPEC strains and suggests that this diversity may influence in the disease severity.

关键词： E.coli Diarrhea Virulence Factors

Antimicrobial and Antioxidant Activity of Anacardium occidentale L. Flowers in Comparison to Bark and Leaves Extracts

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Journal of Biosciences and Medicines 2016年第4期4卷 87-99页

作者： Rubenice Amaral da Silva Silvana Amado Liberio Flavia M. M. do Amaral Flavia Raquel Fernandes do Nascimento Luce Maria Brandão Torres valério monteiro neto Rosane Nassar Meireles Guerra Laboratory of Immunophysiology Universidade Federal do Maranhao Campus do Bacanga Sao Luis Brazil Instituto Botanico de Sao Paulo Sao Paulo Brazil Departament of Pathology Universidade Federal do Maranhao Sao Luis Brazil Universidade Ceuma Sao Luís Brazil

Anacardium occidentale L., Anacardiaceae, is frequently used to treat infections. We evaluated the antimicrobial and antioxidant activity of extracts obtained from aerial parts of the plant in comparison with the extract prepared with the flowers. Ethanol extracts of the leaves (EEL), stem bark (EEB), and flowers (EEF) were prepared separately. The antimicrobial activity was evaluated by agar diffusion and broth dilution methods. The minimum bactericidal and fungicidal concentrations were determined against: Streptococcus mutans, Lactobacillus acidophilus, Staphylococcus aureus, MRSA, Enterococcus faecalis, Streptococcus pyogenes, Pseudomonas aeruginosa, Proteus mirabilis, Escherichia coli, Klebsiella pneumoniae, Helicobacter pylori, Salmonella choleraesuis, Candida albicans, and Candida tropicalis. The antioxidant activity of the extracts was evaluated based on their scavenger capacity of 2,2-diphenyl-1-picrylhydrazyle. The phytochemical profile was analyzed by colorimetric methods and by HPLC with UV detection. All extracts exhibited antioxidant and antimicrobial activity. Although, the EEF was the most effective since it inhibited the growth of all 14 microorganisms tested. Anacardic and galic acids were identified in all extracts as well phenolic compounds, triterpenes, flavones and xanthones. In conclusion, the extracts obtained from the aerial parts of A. occidentale, mainly the extract of flowers that was the most effective, are rich in bioactive metabolites that exert a potent antioxidant and antimicrobial effect. Taken together, the results indicate an important biotechnological potential of A. occidentale as a source of compounds with broad-spectrum antimicrobial and of antioxidant activity to be used in the food and cosmetics industry.

关键词： Anacardium occidentale Antimicrobial Agents Flowers Cashew Caju Antioxidant

Flight performance and wing morphology in the bat Carollia perspicillata:biophysical models and energetics

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Integrative Zoology 2023年第5期18卷 876-890页

作者： Lucas de Oliveira CARNEIRO Breno MELLADO Marcelo Rodrigues NOGUEIRA Ariovaldo Pereira da CRUZ-neto Leandro Rabello monteiro Laboratório de Ciências Ambientais Centro de Biociências e BiotecnologiaUniversidade Estadual do Norte FluminenseCampos dos GoytacazesRio de JaneiroBrasil Departamento de Biodiversidade Universidade Estadual PaulistaRio ClaroSão PauloBrasil

Studies on functional performance are important to understand the processes responsible for the evolution of *** trait variation within species influences the energetic cost of locomotion and impacts life history traits,with ecological and evolutionary *** study examined wing morphology correlates of flight performance measured by energetic expenditure in the Seba’s short-tailed bat,Carollia *** the flight experiments,nature caught bats(59 females,57 males)were allowed to fly for 3 min in a *** each flight,thermographic images were taken to measure body temperature,and biophysical models were used to calculate sensible heat loss as a measure of energetic *** morphological traits were measured for each individual and associated with heat loss and power required to fly on performance *** morphological traits explained 7-10%of flight energetic cost,and morphologies with the best performance would save the energy equivalent to 9-30%of total daily *** optimal performance areas within the *** morphospace were consistent with predicted selection trends from the literature.A trade-off between demands for flight speed and maneuverability was *** loading and camber presented sexual *** morphological differences are likely associated with more economical but less maneuverable flight in females,leading them to fly more often in open areas along the forest *** findings demonstrate how small scale changes in wing morphology can affect life history strategies and fitness.

关键词： aerodynamics Chiroptera infrared thermography performance surfaces Phyllostomidae wing morphometrics

Osteoblasts are inherently programmed to repel sensory innervation

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Bone Research 2020年第3期8卷 343-355页

作者： Luis Leitao Estrela neto Francisco Conceicao Ana monteiro Marina Couto Cecilia JAlves Daniela MSousa Meriem Lamghari Instituto de Investigacao e Inovacao em Saude(i3S) Universidade do Porto4200-135 PortoPortugal Instituto de Engenharia Biomedica(INEB) Universidade do Porto4200-135 PortoPortugal Instituto de Ciencias Biomedicas Abel Salazar(ICBAS) Universidade do Porto4050-313 PortoPortugal

Tissue innervation is a complex process controlled by the expression profile of signaling molecules secreted by tissue-resident cells that dictate the growth and guidance of *** innervation is part of the neuronal network of the bone tissue with a defined spatiotemporal occurrence during bone ***,the current understanding of the mechanisms regulating the map of sensory innervation in the bone tissue is still ***,we demonstrated that differentiation of human mesenchymal stem cells to osteoblasts leads to a marked impairment of their ability to promote axonal growth,evidenced under sensory neurons and osteoblastic-lineage cells *** mechanisms by which osteoblast lineage cells provide this nonpermissive environment for axons include paracrine-induced repulsion and loss of neurotrophic factors *** identified a drastic reduction of NGF and BDNF production and stimulation of Sema3A,Wnt4;and Shh expression culminating at late stage of OB *** noted a correlation between Shh expression profile,OB differentiation stages,and OB-mediated axonal *** of Shh activity and signaling reversed the repulsive action of osteoblasts on sensory ***,to strengthen our model,we localized the expression of Shh by osteoblasts in bone ***,our findings provide evidence that the signaling profile associated with osteoblast phenotype differentiating program can regulate the patterning of sensory innervation,and highlight osteoblast-derived Shh as an essential player in this cue-induced regulation.

关键词： stimulation innervation reversed

Addition of respiratory exercises to conventional rehabilitation for children and adolescents with cerebral palsy:a systematic review and meta-analysis

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World Journal of Pediatrics 2023年第4期19卷 340-355页

作者： Thálita Raysa de Lima Crispim Mansueto Gomes neto Tafnes Rayane Lima Crispim Renivaldo Batista Dias Maria Déborah monteiro de Albuquerque Micheli Bernardone Saquetto Paulo André Freire Magalhães Research Group of Neonatal and Pediatric Physical Therapy Baby GrUPEPetrolinaPernambucoBrazil Postgraduate Program in Rehabilitation and Functional Performance PPGRDFUniversidade de PernambucoRodovia BR 203Km 2 S/N.Vila EduardoPetrolinaPernambuco56328-900Brazil Research Group of Physiotherapy Department of Physical TherapyUniversidade Federal da BahiaSalvadorBrazil Posgraduate Program in Medicine and Health SalvadorBahiaBrazil

Background Respiratory dysfunctions are an important cause of morbidity and death in cerebral palsy(CP)*** exercises in addition to conventional rehabilitation have been suggested to improve respiratory status in CP *** objective of this systematic review and meta-analysis was to verify the effects of the addition of respiratory exercises to conventional rehabilitation on pulmonary function,functional capacity,respiratory muscle strength,gross motor function and quality of life in children and adolescents with *** We searched for randomized controlled clinical trials in PubMed/Medline,Lilacs,SciELO,EMBASE and Physi-otheraphy Evidence(PEDro)from their inception until July 2022 without language *** that included respiratory exercises(breathing exercise program;feedback respiratory training;incentive spirometer exercise;inspiratory muscle training;and combination of respiratory exercises+incentive spirometer exercise)in combination with conventional rehabilitation for children and adolescents with CP were evaluated by two independent *** mean difference(MD)and 95%confidence interval(CI)were estimated by random effect *** Ten studies met the eligibility criteria,including 324 children aged from 6 to 16 *** meta-analysis showed an improvement in inspiratory muscle strength of 22.96 cmH2O(18.63-27.27,n=55)and pulmonary function of 0.60(0.38-0.82,n=98)for forced vital capacity(L);0.22(0.06-0.39,n=98)for forced expiratory volume at 1 second(L);and 0.50(0.05-0.04,n=98)for peak expiratory flow(L/min).Functional skills in daily living activities improved in the intervention ***'assistance of daily living activities,functional capacity,gross motor function and expiratory muscle strength showed a nonsignificant *** well-being and acceptance and functioning domains improved in only one *** Emerging data show significant enhancements in inspiratory muscle stren

关键词： Breathing exercises Cerebral palsy Inspiratory muscle training Pediatrics Rehabilitation Respiratory exercise

The Difference of the Impact of Elective Cholecystectomy Surgery in the Immune Response

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Surgical Science 2018年第8期9卷 243-247页

作者： Rosamaria Rodrigues Gomes Manoel Messias Lima neto Ayrton Silva Alves Cristiane monteiro da Cruz Medical School Centro Universitário Cesmac Maceió Brazil

Introduction: The inflammatory response is essential to initiate the healing process, and in this response there is an increase in white blood cells and pro-inflammatory compounds. Creactive proteins (CRP), together with the blood leukocyte count, have been used to study the immune response. Due to the risk of infection and exacerbated inflammatory response of the patients undergoing surgical procedure, it is relevant to investigate the pre and postoperative inflammatory response of those individuals. Objective: To determine the difference in the impact of elective cholecystectomy surgery on the patient’s immune response. Methods: The present study was cross-sectional, where a group included patients who underwent elective cholecystectomy procedure. Patients who were pregnant, under 18 years, of indigenous communities or with other conditions that depress the immune system, as well as those on immunosuppressive medications, were excluded. Results: CRP and leukocyte counts indicated a mean increase of 5.97-fold (95% CI 3.76 to 8.34, SD 6.98) and 1.97-fold (95% CI 1.71 to 2.24, SD 1.97) respectively, both being evaluated before and after surgery. Discussion: In the present study, to avoid a possible methodological bias, it was chosen to evaluate the patients submitted to videolaparoscopic cholecystectomy only and the hypothesis was confirmed that there is an increase in CRP and leukocyte count as a response to surgical trauma. Conclusion: There was an elevation of the inflammatory markers in patients submitted to surgical trauma when comparing the exams collected in the preoperative period and after the surgical aggression.

关键词： Cholecystectomy Inflammation Leukocytes C-Reactive Protein

Antimicrobial Susceptibility,Biofilm Production and Adhesion to HEp-2 Cells of Pseudomonas aeruginosa Strains Isolated from Clinical Samples

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Journal of Biomaterials and Nanobiotechnology 2013年第1期4卷 98-106页

作者： Alicia valeria Zaranza Francyelle Costa Morais Monique Santos do Carmo Adriana de Mendonca Marques Cristina Andrade-monteiro Thiago Feitosa Ferro valerio monteiro-neto Patricia de Maria Silva Figueiredo Research Center in Parasitic Diseases and Endemic University of CEUMAUniCEUMASao LuisBrazil

A hundred Pseudomonas aeruginosa strains from several clinical specimens from five hospitals in Sao Luís-MA were evaluated for biofilm production, prevalence of the gene algD, adhesion to HEp-2 cells and antimicrobial susceptibility. The most affected clinical specimens and hospital sectors were also evaluated. Most isolates were obtained from the tracheal aspirate (21.0%) and the most affected hospital sector was the ICU (43.0%). The antibiotics with the highest sensitivity rate were amikacin, piperacillin/tazobactam, fluoroquinolones, gentamicin and meropenem and the ones with the highest resistance rate were aztreonam, ceftazidime and cefepime. All samples were sensitive to polymyxin B. In relation to the expression of the gene for ESBL, 50.0% (17/34) of the multiresistant strains showed the enzyme TEM. Most strains showed high hydrophobicity and 96% of the isolates produced biofilm on a polystyrene microplate, 52% were capsule producers, 19% showed mannose-sensitive fimbriae and 39% expressed the gene algD. We observed adhesion to HEp-2 cells and to the coverslip. These factors may be reported in the pathogenesis of this bacterium, what represents a potential risk for colonization of medical devices which favor the establishment of chronic nosocomial infections.

关键词： Pseudomonas aeruginosa Antimicrobial Biofilm Adhesion

Enterobacteria and Vibrio from Macrobrachium amazonicum prawn farming in Fortaleza,Ceará,Brazil

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Asian Pacific Journal of Tropical Medicine 2016年第1期9卷 24-28页

作者： Débora de Souza Collares Maia Castelo-Branco Jamille Alencar Sales Raimunda Samia Nogueira Brilhante Glaucia Morgana de Melo Guedes Yago Brito de Ponte Celia Maria de Souza Sampaio Tereza de Jesus Pinheiro Gomes Bandeira José Luciano Bezerra Moreira Lucas Pereira de Alencar Manoel de Araújo neto Paiva Rossana de Aguiar Cordeiro André Jalles monteiro Waldemiro de Aquino Pereira-neto José Júlio Costa Sidrim Marcos Fábio Gadelha Rocha Department of Pathology and Legal Medicine Postgraduate Program in Medical MicrobiologySpecialized Medical Mycology CenterFederal University of Ceará School of Veterinary Medicine Postgraduate Program in Veterinary SciencesState University of Ceará Department of Statistics and Applied Mathematics Federal University of Ceará

Objective:To investigate the isolation of enterobacteria associated with Macrobrachium amazonicum(***) farming and evaluate the in vitro antimicrobial susceptibility of Vibrio ***:Strains were isolated from female *** prawns and environmental and hatchery *** assays were used to identify bacterial genera and those belonging to the genus Vibrio were submitted to further analyses for species identification,through Vitek 2 automated system and *** test was performed according to Clinical Laboratory Standards ***:The following genera of enlerobacteria were recovered:Enterobacter(n=11),Citrobacter(n=10),Proteus(n=2),Serratia(n=2),Kluyvera(n=2),Providencia(n=2),Cedecea(n=1),Escherichia(n=1).Edwardsiella(n=1) and BulliauxeUa(n=1).As for Vibrio,three species were identified:Vibrio cholerae non-O1/non-O139(n=4).Vibrio vulnificus(***)(n=1) and Vibrio mimicus(n=1).Vibrio *** minimum inhibitory concentrations values within the susceptibility range established by Clinical Laboratory Standards Institute for almost all antibiotics,except for ***,which presented intermediate profile to ***:Enterobacteria do not seem to be the most important pathogens associated with *** farming,whereas the recovery of Vibrio *** larviculture,with emphasis on Vibrio cholerae and ***,deserves special attention due to their role as potentially zoonotic aquaculture-associated ***,the intermediate susceptibility of *** to ampicillin reflects the importance of monitoring drug use in prawn farming.

关键词： Prawn Water Enterobacteria Vibrio Antibiotics

Six-Minute Walk Test in Patients with Rheumatoid Arthritis

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Open Journal of Rheumatology and Autoimmune Diseases 2019年第1期9卷 14-24页

作者： Sandor Balsamo Ana Paula monteiro Gomides Licia Maria Henrique da Mota Frederico Santos de Santana Raphaela Franco Miranda Talita Yokoy Luciana Muniz Leopoldo Luiz Santos-neto Universidade de Brasília (UnB) Brasília Brazil Laboratório de Aptidã o Física e Reumatologia do Hospital Universitário de Brasília (LAR/HUB) Brasília Brazil Grupo de Estudo e Pesquisa em Exercício de Forç a e Saúde (GEPEEFS) Brasília Brazil Curso Superior de Educaç o Física Centro Universitário UNIEURO Brasília Brazil Ambulatório de Reumatologia Hospital Universitário de Brasília (HUB) Brasília Brazil

Background: The 6-minute walk test (6TC) was initially used as an instrument for assessing physical and cardiorespiratory capacity, but is currently being used to monitor treatments, and compare physical interventions and prognostic evaluation. Although already recognized as a research method in several specialties, 6TC has not been used in rheumatology. Patients with rheumatoid arthritis (RA) may have impaired functional capacity as well as increased cardiovascular mortality. An adequate functional evaluation of these patients is necessary and the 6TC may be useful in this sense. Objective: The aim of this study was to perform the 6-minute walk test in RA patients and to compare the performance with a control group. Method: A cross-sectional study was carried out in which the sample consisted of 85 women, 46 patients with rheumatoid arthritis and 39 healthy controls. A descriptive analysis of the data was performed. One-Way ANOVA methodology was used to compare the patient and control groups followed by the graphic analysis. Results: The distance walked on the 6TC by RA patients was on average 522.2 meters. In the distance control group found in the 6TC was on average 628.8 meters, the difference being statistically significant. Conclusion: In this study the distance covered in 6TC by women with RA was lower than that of healthy women of the same age.

关键词： 6-Minute Walk Test Rheumatoid Arthritis Functional Capacity Physical Capacity Cardiorespiratory Capacity Person Performed

Virulence properties of a peptide hemolysin produced by Enterococcus faecalis

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Advances in Bioscience and Biotechnology 2012年第7期3卷 909-917页

作者： M.T.Furumura P.M.S.Figueiredo G.V.Carbonell A.L.Darini Maria Rosa Quaresma Bomfim Rosimary de Jesus Gomes Turri Francyelle Costa Moraes Cristina de Andrade monteiro Herminio Benitez Rabello Mendes valerio monteiro neto T.Yano Department of Microbiology and Immunology Biological InstituteUniversidade Estadual de CampinasCampinasBrazil Research Group of Endemic and Parasitic Diseases CEUMA UniversitySao LuisBrazil Medical Biology Adolfo Lutz InstitutCampinasBrazil Department Clinical Toxicologicals and Bromatologicals AnalisysUSPRibeirao PretoBrazil Federal University of Maranhao Sao LuisBrazil

The characterization and prevalence of virulence factors associated with enterococcal invasiveness and severity of disease are important areas to be investigated. Recently, we described the production of a heat-stable hemolysin by clinical isolates of Enterococcus faecalis cultived in BHI-GA (BHI with glucose and L-arginine). Now, we purified the hemolysin from the culture supernatant by ultra-filtration (PM-10 membrane) and ethanol extraction followed by chromatography in a mBondapak C18 and Superdex Peptide columns. The hemolytic activity was not affected by the proteolytic enzymes. Cholesterol, phospholipids, EDTA and also bivalent ions did not inhibit the hemolytic activity. Among the various carbohydrates, only dextran 4 protected the erythrocytes against lyse. Scanning electron microscopy showed that lyse of erythrocytes occured at once after the exposure to the hemolysin. The mito-chondrial activity and the cell membrane integrity were significantly affected by the hemolysis, within 20 min of exposure and caused apoptosis after 12 h incubation, 51.92% in HeLa and 68% in HEp-2 cells, analyzed by flow cytometry. These results suggest that the heat-stable pore forming hemolysin might be a putative virulence factor in enterococci infections.

关键词： Enterococcus Faecalis Cytotoxin Heat-Stable Hemolysin Human Epithelial Cells Apoptosis