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Antibiotic Resistance Profile of Enterococcus faecalis and Enterococcus faecium Isolates from Urine and Pleural Fluid in Two Hospitals of Cameroon

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Open Journal of Medical Microbiology 2024年第1期14卷 39-49页

作者： Cécile Ingrid Djuikoue Farid Wega Grace Yemina Kombeu Cedric Seugnou Nana Christiane Possi Kiyang Lussabella Pohoko Medzam Erica Megane Noubissi Makougoum Nellie Mbianga Nkuigoua Herman Koyouo Tagne Mérimé Joël Tchinda Talokou Yvonne valérie Yolande Mavoungou Ashu Michel Agbor Microbiology Department of the Faculty of Health Sciences of the Université des Montagnes Bangangté Cameroon Faculty of Medicine and Pharmaceutical Sciences University of Douala Douala Cameroon Microbiology Department of the Faculty of Health Sciences of Institut Universitaire du Golf de Guinée Douala Cameroon Centre Hospitalier Dominicain St Martin de Porres Yaoundé Cameroun Faculté des Sciences de la Santé Université Marien Ngouabi Brazzaville République du Congo

Enterococcus faecalis and Enterococcus faecium rank among the leading causes of nosocomial bacteremia and urinary tract infections. They often persist on hospital surfaces due to their ability to withstand adverse environmental conditions (low or high temperatures, high pH, and high salinity). The global Enterococcus faecalis-Enterococcus faecium ratio is currently shifting towards Enterococcus faecium. Enterococci present variable levels of resistance to certain families of antibiotics. This is the case for aminoglycosides, beta-lactams and cephalosporins. In 2017, WHO ranked Enterococci among priority pathogens for research and development of new antibiotics. The objective of our study was to determine the antibiotic resistance profile of Enterococcus faecalis and Enterococcus faecium isolates from urine and pleural fluid in two hospitals in Cameroon. This cross-sectional and analytic study was carried out between June to August 2023 on hospitalized and day patients in which a cytobacteriological test of urine and pleural fluid was done. The samples were inoculated on CLED Agar for urine and on Chocolate + polyvitex and blood agar (prepared from Columbia agar) for pleural fluid samples and incubated at 37℃ for 18 to 24 hours. Identification of isolates was carried out using the API 20 STREP micro gallery (Biomerieux, France) and tested for antimicrobial susceptibility. The data on socio-demographical and potential risk factors were recorded using self-administered questionnaires and data from laboratory analyses of the specimen were collected in a data capture sheet. Potential risk factors associated with the presence of Enterococci, were evaluated using the logistic regression in univariate and multivariate analysis. P value < 0.05 was considered as significant. A total of 511 patients were recruited who were predominantly females. Enterococcus spp were isolated in 27.79% of our samples with Enterococcus faecalis mostly encountered. Enterococcus spp showed a high

关键词： Enterococcus faecalis Enterococcus faecium Healthcare-Associated Infections Antimicrobial Resistance Cameroon

Electrochemiluminescence in Thermo-Responsive Hydrogel Films with Tunable Thickness

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Journal of Analysis and Testing 2020年第2期4卷 107-113页

作者： Haidong Li valérie Ravaine Neso Sojic Univ.Bordeaux Bordeaux INPISM CNRS UMR 5255Site ENSCBP33607 PessacFrance School of Chemistry and Chemical Engineering Yangzhou UniversityYangzhou 225002China

Electrochemiluminescence(ECL)has attracted considerable interest for many applications such as microscopy,(bio)analysis,light-emitting materials or *** this work,we report the fabrication and characterization of ECL-active hydrogel films with tunable *** redox films were prepared by electrochemically-assisted radical polymerization by potential cycling of a PBS solution containing the monomer N-isopropylacrylamide,the initiator potassium persulfate,the cross-linker N,N′-methylenebis(acrylamide)and the Ru(bpy)3 *** deposits were easily prepared in a rapid and well-controlled one-step *** resulting homogeneous films are composed of a poly(N-isopropylacrylamide)(pNIPAM)matrix,which incorporates covalently[Ru(bpy)_(3)]^(2+)*** thickness and the number of ECL-active sites is tuned by control-ling the number of voltammetric *** deposited pNIPAM films are permeable to water-soluble chemicals such as the coreactant tri-n-propylamine(TPrA).The voltammetric characterization shows a continuous increase of the number of redox-active *** indicate that ECL signals are proportional to the number of electrodeposited[Ru(bpy)_(3)]^(2+)*** approach combining ECL and stimuli-responsive hydrogels open exciting prospects for developing new(bio)sensing materials.

关键词： Electrochemiluminescence Hydrogel pNIPAM film [Ru(bpy)_(3)]^(2+) Coreactant Electrochemically-assisted radical polymerization

Inverse relationship between platelet Akt activity and hippocampal atrophy:A pilot case-control study in patients with diabetes mellitus

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World Journal of Clinical Cases 2024年第2期12卷 302-313页

作者： Haruhiko Tokuda Takamitsu Hori Daisuke Mizutani Tomoyuki Hioki Kumi Kojima Takashi Onuma Yukiko Enomoto Tomoaki Doi rie Matsushima-Nishiwaki Shinji Ogura Hiroki Iida Toru Iwama Takashi Sakurai Osamu Kozawa Department of Metabolic Research Research InstituteNational Center for Geriatrics and GerontologyObu 474-8511Japan Department of Clinical Laboratory National Center for Geriatrics and GerontologyObu 474-8511Japan Department of Pharmacology Gifu University Graduate School of MedicineGifu 501-1194Japan Department of Neurosurgery Gifu University Graduate School of MedicineGifu 501-1194Japan Department of Dermatology Central Japan International Medical CenterMinokamo 505-8510Japan Department of Anesthesiology and Pain Medicine Gifu University Graduate School of MedicineGifu 501-1194Japan Department of Emergency Medicine Faculty of MedicineUniversity of ToyamaToyama 930-0194Japan Department of Emergency and Disaster Medicine Gifu University Graduate School of MedicineGifu 501-1194Japan Research Institute National Center for Geriatrics and GerontologyObu 474-8511Japan

BACKGROUND Akt plays diverse roles in *** is involved in the pathogenesis of type 2 diabetes mellitus(T2DM),which is caused by insulin *** also plays a vital role in human platelet ***,the hippocampus is closely associated with memory and learning,and a decrease in hippocampal volume is reportedly associated with an insulin-resistant phenotype in T2DM patients without *** To investigate the relationship between Akt phosphorylation in unstimulated platelets and the hippocampal volume in T2DM *** Platelet-rich plasma(PRP)was prepared from the venous blood of patients with T2DM or age-matched *** pellet lysate of the centrifuged PRP was subjected to western blotting to analyse the phosphorylation of Akt,p38 mitogen-activated protein(MAP)kinase and glyceraldehyde 3-phosphate dehydrogenase(GAPDH).Phosphorylation levels were quantified by densitometric *** volume was analysed using a voxel-based specific regional analysis system for Alzheimer’s disease on magnetic resonance imaging,which proposes the Z-score as a parameter that reflects hippocampal *** The levels of phosphorylated Akt corrected with phosphorylated p38 MAP kinase were inversely correlated with the Z-scores in the T2DM subjects,whereas the levels of phosphorylated Akt corrected with GAPDH were ***,this relationship was not observed in the control *** These results suggest that an inverse relationship may exist between platelet Akt activation and hippocampal atrophy in T2DM *** findings provide insight into the molecular mechanisms underlying T2DM hippocampal atrophy.

关键词： Akt Platelet Hippocampal atrophy Magnetic resonance imaging Diabetes mellitus

Molecular Diagnosis of Sexual Differentiation Disorders in Burkina Faso

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Journal of Biosciences and Medicines 2024年第3期12卷 1-15页

作者： Bazie Bapio valérie Elvira Jean Télesphore Sawadogo Soumaïla Zohoncon Théodora Mahoukèdè Tall Ahmadou Bado Prosper Soubeiga Serge Théophile Ouattara Moussa Sorgho Abel Kiendrebeogo Touwendpoulimdé Isabelle Yonli Albert Theophane Djigma Florencia Millogo/Traore Timongo Françoise Danielle Simpore Jacques Centre National de la Recherche Scientifique et Technologique (CNRST) Institut de Recherche en Sciences de la Santé (IRSS) Ouagadougou Burkina Faso Université Joseph KI-ZERBO Unité de Formation en Sciences de la Vie et de la Terre Laboratoire de Biologie Moléculaire et de Génétique (LABIOGENE) Ouagadougou Burkina Faso Centre de Recherche Biomoléculaire Pietro Annigonie (CERBA) Ouagadougou Burkina Faso Centre Hospitalier Universitaire Régional de Ouahigouya Ouagadougou Burkina Faso Centre Hospitalier Universitaire Yalgado Ouedraogo Université Joseph Ki-Zerbo Ouagadougou Burkina Faso

Sex development anomalies represent a group of congenital pathologies in which chromosomal, gonadal and anatomical sex differentiation is atypical. The aim of our study was to use molecular biology techniques to elucidate sex in cases of anomalies of sexual differentiation in Burkina Faso. This cross-sectional study took place from March 2023 to June 2023. Oral and blood samples were collected respectively using sterile swabs and stored on Swab Kits and NUCLEIcardTM (https://***/product-ranges/nucleic-card/) were used to determine gonosome profiles. Extraction was carried out using the DNA Swap solution Kit and the DNA IQ System Kit, and a PowerPlex? 21 kit (Promega) for amplification. The Applied Biosystems 9700 thermal cycler was used for PCR followed by 36 cm capillary electrophoresis in the Applied Biosystems 3130 prism sequencer. Sequence files were analyzed using GeneMapper IDX v. 3.2 software. Seven (07) patients were registered during the study period. There were 4 cases of XX DSD or 46, XX DSD and 3 cases of XY DSD or 46, XY DSD. The median age of our patients was 16 years. Civilian sex was male in 4 cases and female in 3. The most frequent reason for consultation was micropenis in 3 cases, followed by primary amenorrhea and sex ambiguity. There were 03 cases of discrepancy between genetic sex and civil sex. The accessibility of molecular diagnosis is little known to clinicians. XX DSDs or 46, XX DSDs were the most frequent (4/7) in our study. The problem facing this situation is early diagnosis to help prevent complications in Burkina Faso.

关键词： Gonosome DSD Abnormal Sexual Development Burkina Faso

Contribution of SAR Radar Imagery in the Detection of Suspicious Vessels in the Ivorian EEZ

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World Journal of Engineering and Technology 2024年第1期12卷 200-212页

作者： Koffi Cyprien Yoboue Jean-Baptiste Kassi Eric valère Djagoua Tiemelé Jacque-André Laboratory of Remote Sensing and GIS University Center for Research and Application in Remote Sensing (CURAT) Félix Houphouet-Boigny University of Abidjan Cocody Ivory Coast

The present work proposed a method for match SAR and AIS data to detect vessels carrying out suspicious activity in the Ivorian EEZ. Two superposition methods, detected AIS and SAR data, based on point-to-point association on the one hand and point-to-line on the other hand, were used to detect suspicious vessels in Ivorian marine waters. The results showed that most vessels detected in the Ivorian EEZ do not declare their positions to avoid being spotted. These funds are likely to practice illegal, undeclared and unregulated fishing (INN fishing). This clandestine activity is very recurrent in Ivorian waters. This is illustrated by the number of suspicious vessels detected by SAR radar imagery which is greater compared to declared or authorized vessels.

关键词： INN Fishing CFAR Algorithm AIS Sentinel-1A Ivory Coast

Detection of liver micrometastases from colorectal origin by perfusion CT in a rat model

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Hepatobiliary & Pancreatic Diseases International 2014年第3期13卷 301-308页

作者： Caroline Hatwell Magaly Zappa Mathilde Wagner Nicolas Michoux valérie Paradis valérie Vilgrain Léon Maggiori Yves Panis Department of Colorectal Surgery Beaujon Hospital (AP-HP) 100 boulevard du Général Leclerc 92118 Clichy France Research Unit Bichat-Beaujon INSERM U773 Université Paris VII (Denis Diderot) Paris France Department of Radiology Beaujon Hospital (AP-HP) 100 boulevard du Général Leclerc 92118 Clichy France Department of Pathology Beaujon Hospital (AP-HP) 100 boulevard du Général Leclerc 92118 Clichy France

BACKGROUND: Some patients with colorectal carcinoma have liver metastases(LMs) which cannot be detected by conventional imaging. This study aimed to assess whether hepatic perfusion changes induced by micrometastases can be detected by perfusion computed tomography(CT).METHODS: LMs were produced in rats by injecting carcinoma cells into the portal vein. Perfusion CT was performed at microscopic(day 10), interval(day 17), and macroscopic stage(day 34). Perfusion parameters were computed using a dualinput one-compartmental ***: Micro and macro LMs presented a mean diameter of 0.5 and 2.6 mm, respectively. Compared to controls, LMs at interval(1.1 mm) and macroscopic stage induced significant perfusion changes: a decrease of 42%(P=0.004) and 41%(P=0.029) in hepatic transit time and an increase of 292%(P=0.073) and 240%(P=0.001) in portal delay, ***: LMs with a mean diameter between 1.1 and2.6 mm induced significant hepatic perfusion changes, detected by CT. Such detection may help to select patients and propose chemotherapy at the time of primary tumor resection.

关键词： liver circulation colorectal liver micrometastasis perfusion imaging computed tomography

Study of Rice Husks and Expanded Polystyrene Composites for Construction Applications

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Open Journal of Civil Engineering 2024年第3期14卷 456-468页

作者： Edem Chabi valéry Kouandété Doko Sena Peace Hounkpè Paul Damien Amadji Emmanuel Olodo Laboratory of Rural Engineering School of Rural Engineering National University of Agriculture Ketou Benin Laboratory of Applied Energy and Mechanics (LEMA) Polytechnic School of Abomey-Calavi University of Abomey-Calavi Abomey-Calavi Benin Department of Civil Engineering Higher National School of Public Works (ENSTP) National University of Sciences Technologies Engineering and Mathematics Abomey Benin

In the current context of environmental challenges, this study focuses on developing innovative and eco-friendly composites using rice husk and recycled expanded polystyrene. This dual-responsibility approach valorizes a by-product like rice husk, often considered waste, and reuses polystyrene, a plastic waste, thereby contributing to CO2 emission reduction and effective waste management. The manufacturing process involves dissolving recycled polystyrene into a solvent to create a binder, which is then mixed with rice husk and cold-compacted into composite materials. The study examines the impact of two particle sizes (fine and coarse) and different proportions of recycled polystyrene binder. The results show significant variations in the mechanical characteristics of the composites, with Modulus of Rupture (MOR) values varying from 2.41 to 3.47 MPa, Modulus of Elasticity (MOE) ranging from 223.41 to 1497.2 MPa, and Stiffness Coefficient (K) from 5.04 to 33.96 N/mm. These characteristics demonstrate that these composites are appropriate for various construction applications, including interior decoration, panel claddings, and potentially for furniture and door manufacturing when combined with appropriate coatings. This study not only highlights the recycling of agricultural and plastic waste but also provides a localized approach to addressing global climate change challenges through the adoption of sustainable building materials.

关键词： Rice Husk Recycled Expanded Polystyrene Eco-Friendly Composites Waste valorization Sustainable Construction

Performance Evaluation of Novel Eco-Materials Composed of Millet Husks, Rice Husks, and Polystyrene

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Open Journal of Composite Materials 2024年第1期14卷 33-43页

作者： Paul Damien Amadji Edem Chabi valéry Kouandété Doko Céphas Houndedji Emmanuel Olodo Laboratory of Applied Energy and Mechanics (LEMA) Polytechnic School of Abomey-Calavi University of Abomey-Calavi Abomey-Calavi Benin Laboratory of Rural Engineering School of Rural Engineering National University of Agriculture Kétou Benin

Managing agricultural waste and expanded polystyrene (EPS) poses significant environmental and economic challenges. This study aims to create composites from millet husks, rice husks, and recycled EPS, using a manufacturing method that involves dissolving the polystyrene in a solvent followed by cold pressing. Various particle sizes and two binder dosages were investigated to assess their influence on the physico-mechanical properties of the composites. The mechanical properties obtained range from 2.54 to 4.47 MPa for the Modulus of Rupture (MOR) and from 686 to 1400 MPa for the Modulus of Elasticity in Bending (MOE). The results indicate that these composites have potential for applications in the construction sector, particularly for wood structures and interior decoration. Moreover, surface treatments could enhance their durability and mechanical properties. This research contributes to the valorization of agricultural and plastic waste as eco-friendly and economical construction materials.

关键词： Bio-Based Composites Millet Husk Rice Husk Expanded Polystyrene Waste valorization Sustainable Construction

The interplay between DNA topoisomerase 2a posttranslational modifications and drug resistance

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Cancer Drug Resistance 2020年第2期3卷 149-160页

作者： Christophe Lotz valérie Lamour Integrative Structural Biology Department IGBMCUniversitéde StrasbourgCNRS UMR 7104INSERM U1258Illkirch 67404France Hôpitaux Universitaires de Strasbourg Strasbourg 67000 France

The type 2 DNA topoisomerases(Top2)are conserved enzymes and biomarkers for cell *** catalytic activities of the human isoform Top2a are essential for the regulation of DNA topology during DNA replication,transcription,and chromosome ***2a is a prominent target for anti-cancer drugs and is highly regulated by post-translational modifications(PTM).Despite an increasing number of proteomic studies,the extent of PTM in cancer cells and its importance in drug response remains largely *** this review,we highlight the different modifications affecting the human Top2a in healthy and cancer cells,taking advantage of the structure-function information accumulated in the past *** also overview the regulation of Top2a by PTM,the level of PTM in cancer cells,and the resistance to therapeutic compounds targeting the Top2 ***,this review underlines the importance of future studies addressing more systematically the interplay between PTM and Top2 drug resistance.

关键词： DNA topoisomerase drug resistance post-translational modifications etoposide