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A rice tubulin tyrosine ligase-like 12 protein affects the dynamic and orientation of microtubules

Journal of Integrative Plant Biology 2021年第5期63卷 848-864页

作者： Kunxi Zhang Xin Zhu Steffen Durst Petra Hohenberger Min‐Jung Han Gynheung An vaidurya psahi Michael Riemann Peter Nick Molecular Cell Biology Botanical InstituteKarlsruhe Institute of TechnologyKarlsruhe 76131Germany Aptamer Initiative Postech Biotech CenterPohang University of Science and TechnologyPohang‐siGyeongsangbuk‐do37673South Korea Department of Plant Molecular Systems Biotech Kyung Hee UniversityYongin 446‐701South Korea

The detyrosination/retyrosination cycle is the most common post-translational modification of α-*** of the conserved C-terminal tyrosine of α-tubulin by a still elusive tubulin tyrosine carboxypeptidase, and religation of this tyrosine by a tubulin tyrosine ligase(TTL), are probably common to all eukaryotes. Interestingly, for plants, the only candidates qualifying as potential TTL homologs are the tubulin tyrosine ligase-like 12 proteins. To get insight into the biological functions of these potential TTL homologs, we cloned the rice TTL-like 12 protein(Os TTLL12)andgeneratedoverexpression Os TTLL12-RFP lines in both rice and tobacco BY-2 cells. We found, unexpectedly, that overexpression of this Os TTLL12-RFP increased the relative abundance of detyrosinated α-tubulin in both coleoptile and seminal root, correlated with more stable microtubules. This was independent of the respective orientation of cortical microtubule, and followed by correspondingly changing growth of coleoptiles and seminal roots. A perturbed organization of phragmoplast microtubules and disoriented cell walls were further characteristics of this phenotype. Thus, the elevated tubulin detyrosination in consequence of Os TTLL12 overexpression affects structural and dynamic features of microtubules, followed by changes in the axiality of cell plate deposition and, consequently, plant growth.

关键词： microtubule phragmoplast rice tobacco BY-2 tubulin detyrosination tubulin tyrosine carboxypeptidase tubulin tyrosine ligase tubulin tyrosine ligase-like 12

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