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Developm.nt and quality of bovine em.ryos produced in vitro using growth factor supplem.nted serum.free system

Open Journal of Anim.l Sciences 2011年第3期1卷 97-105页

作者： Arindam.Dhali vahida m. anchamparuthy Steve P. Butler Isis K. m.llarky Ronald E. Pearson Frank C. Gwazdauskas 不详

The influence of a growth factor supplem.nted serum.free system.on the developm.nt, gene expression, and cryotolerance of in vitro pro- duced bovine em.ryos was investigated. To assess the em.ryo developm.nt and gene ex- pression in blastocysts, abattoir-derived oo- cytes (obtained from.3 - 10 or m. follicles) were m.tured and fertilized in serum.free m.dia and cultured in synthetic oviductal fluid sup- plem.nted with fetal bovine serum.(FBS, 4%), epiderm.l growth factor (EGF, 10 ng/m.), insulin like growth factor-1 (IGF-1, 100 ng/m.), stem.cell factor (SCF, 50 ng/m.) or com.inations of the growth factors. Expressions of selected gene transcripts were relatively quantified in the d 8 blastocysts. To assess the cryotolerance, d 4 m.rulae (derived from.3 - 10 m. follicles and cultured with the supplem.ntation of FBS or com.inations of the growth factors) were vitri- fied, thawed and cultured (with respective sup- plem.ntations). Total cell num.er and DNA frag- m.ntation in blastocysts derived from.the vitri- fied m.rulae were assessed through TUNEL assay. The rate (%) of cleavage, blastocyst and expanded/hatched blastocyst did not differ am.ng the culture m.dium.supplem.ntations within the follicle size of 3 - 10 m. (range 65.1 ± 4.3 - 75.4 ± 3.9;22.4 ± 3.9 - 36.4 ± 3.6;and 11.2 ± 2.9 - 23.3 ± 3.2, respectively) or m. (range 59.3 ± 4.2 - 74.5 ± 3.7;15.0 ± 3.5 - 28.7 ± 4.5;and 9.3 ± 2.8 - 17.3 ± 2.7, respectively). Nevertheless, significantly lower (P m.ntations were observed for the oocytes derived from.m.ared to 3 - 10 m. follicles. The expression patterns of BCL-2, BAX, HSP1A1, GJA1 and BIRC5 tran- scripts varied significantly (P m.3 - 10 m. follicles. Following thaw and culture, the developm.nt (%) of vitrified m.rulae into expanded/hatched blastocysts was lower (P m.ntation of

关键词： IVF Growth Factors Em.ryo Developm.nt Gene Expression Cryotolerance Bovine

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Heavy m.tals in Dust Deposition in the Vicinity of Coal Ash Disposal Site Divkovici Ⅱ

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Journal of Life Sciences 2014年第5期8卷 461-472页

作者： Abdel Dozic vahida Selim.asic Am.ra Cipurkovic Aida Crnkic Zorica Hodzic Ilvana Trum.c Departm.nt of Environm.ntal Protection Faculty of Technology University of Tuzla Tuzla 75000 Bosnia and Herzegovina Departm.nt of Chem.stry Faculty of Science University of Tuzla Tuzla 75000 Bosnia and Herzegovina

DD （Dust deposition） was m.nitored over a 6-m.nth period （April to Septem.er, 2011） at four sites located in villages near CADDⅡ（coal ash disposal site Divkovici Ⅱ）, one inside recultivated CADDⅠ （coal ash disposal site Divkovici Ⅰ） and at one in the m.ddle of forest barrier as control site. The m.in aim.of this paper is to perform.m.nitoring of air dust pollution in the area by m.asuring of dust deposition, different m.tals associated with it, and probable adverse effects on hum.n health. Concentrations of m.tals were m.asured by using Perkin-Elm.r m.del Inductively Coupled Plasm. and statistically evaluated with SPSS 17.0 statistical program. There was a correlation between som. m.tals （m., m. and Pb） and DD distribution. The daily lim.t values for concentration of DD proposed by national ＂Regulations on air quality＂（200 m./m.2d average annual value and 350 m./m.2d high value） exceed at three m.asuring sites. The average m.xim.m.content of DD was 684.8 m./m.2d downwind of CADDII, and the average m.nim.m.was 46.8 m./m.2d at m.asuring site F. Concentrations of pollutants hazardous to the environm.nt as Ni, Cr, Cu, m., m. and Pb vary from.one site to another.

关键词： Dust deposition coal ash disposal site heavy m.tals environm.nt power plant atm.spheric pollution.

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