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Lipid-Protein.Microin.lusion. in.the Morphological Structures of Organ.lle Membran.s Studied by Fluorescen. Con.ocal Microscopy

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Advan.es in.Biological Chemistry 2017年第1期7卷 42-59页

作者： Michael Yu. Chern.shov vadim n. nurminsky n.talia V. Ozolin. Presidium of Irkutsk Scien.ific Cen.er Siberian Branch Russian Academy of Sciences Irkutsk Russia Siberian.In.titute of Plan. Physiology an. Biochemistry Siberian Branch Russian Academy of Sciences Irkutsk Russia

Peculiar properties of morphological structures of organ.lle membran.s were studied by fluorescen. con.ocal microscopy. The list of objects in.our experimen.s was represen.ed by mitochon.ria, chloroplasts an. vacuoles. Durin. this study, iden.ification.of lipid microin.lusion. havin. the form of such lipid-protein.structural microformation. as lipid-protein.microdomain., vesicles an. membran. tubular structures (cytoplasmic tran.vacuolar stran.s an. n.n.tubes) located in.organ.lle membran.s or boun. up with them was con.ucted. Such membran. probes as laurdan. DPH, An. an. bis-An. were used. Comparison.of fluorescen.e in.en.ity of these membran. probes was con.ucted. This in.estigation.of the morphological properties of lipid-protein.structural microformation. was accompan.ed with an.lysis of 1) the phase state an. 2) dyn.mics of microviscosity variation. in.the membran. elemen.s of isolated plan. cell organ.lles. Distribution. of laurdan.fluorescen.e gen.ralized polarization.(GP) values for the membran. on.the whole an. for the in.en.ively fluorescin. membran. segmen.s were obtain.d. It was discovered that the microviscosity of in.en.ively fluorescin. membran. segmen.s essen.ially differed from the microviscosity of the rest part of the membran.. In.con.lusion. some results of the study of peculiar properties of lipid-protein.structural microformation. related to the structure of organ.lle membran.s an. the discoveries made in.this in.estigation.are discussed.

关键词： Iden.ification of Lipids Morphological Structure of Cell Organ.lles Morphological Structure of Vacuolar Membran. Fluorescen. Con.ocal Microscopy Fluorescen. Probes Lipid-Protein Structural Membran. Microin.lusion. Types of Microformation. Iden.ified Lipid-Protein Microdomain. Lipid-Protein Membran. n.n.tubes Vesicles Cytoplasmic Stran.s Microviscosity

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Season.l Dyn.mics of Male an. Female Reproductive Systems in.the Siberian.Salaman.er, Salaman.rella keyserlin.ii (Caudata, Hyn.biidae)

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Asian.Herpetological Research 2015年第3期6卷 169-183页

作者： vadim V.YARTSEV Valen.in. n.KURAn.VA Departmen. of Vertebrate Zoology an. Ecology Institute of BiologyTomsk State University

It is n.t well kn.wn.how low temperatures, like a subarctic steppe–tun.ra climate, in.luen.e reproductive traits of ectothermic vertebrates. To begin.an.werin. this question. we studied male an. female reproductive systems of Salaman.rella keyserlin.ii in.abitin. a Tomsk population.southeast of Western.Siberia）, Russia, in.ecological an. physiological terms. In.males, before spermiation. the testicular size an. weight in.late April–early May were greatest of all. Spermiation.occurred durin. breedin. immigration.in.sprin. when.mean.air temperature was above 10°С, an. at the same time rain.fell. After spermiation. the testicular size an. weight decreased sharply, an. the diameter of the vasa deferen.ia in.reased. ＂Spawn.n.＂（i.e., simultan.ous extrusion.of sperm an. oviposition. occurred from late April to late May, an. this duration.fluctuated in.temperature an. humidity. The testicular size an. weight in.reased in.summer. Sperm mass was detected in.the testes by the smear method in.April–September, except in.Jun. when.sin.le fragmen.ed un.ealized sperm was detected an. in.July when.spermatids were detected. In.females, ovarian.weight was greatest in.sprin. before ovulation. From late Jun., vitellogen.sis began.in.ovarian.follicles, in.which min. green.yolks accumulated. Melan.n.deposited in.the surface of the ovary from July when.oviducts were hypertrophyin.. In.con.rast, some large-sized females did n.t show an. sexual maturity shortly before hibern.tion.although these females may be subadults）. These results suggest that low temperatures in.Siberia in.uce early timin. of gamete maturation.in.females, but the females＇ reproductive cycle might also be bien.ial. A reproductive cycle in.males was an.ual with the completion.of the gamete maturation.process in.August.

关键词： an.ual reproductive cycle bien.ial reproductive cycle breedin. immigration gamete maturation subarctic climate

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Passive vectorin. of en.omopathogen.c fun.us Beauveria bassian. amon. the wax moth Galleria mellon.lla larvae by the ectoparasitoid Habrobracon.hebetor females

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In.ect Scien.e 2018年第4期25卷 643-654页

作者： vadim Yu. Kryukov n.talia A. Kryukova Maksim V. Tyurin.Olga n. Yaroslavtseva Viktor V. Glupov In.titute of Systematics an. Ecology of An.mals Siberian.Bran.h of Russian.Academy of Scien.e Novosibirsk Russia

Females of the ectoparasitoid Habrobracon.hebetor attack an. en.en.mate n.merous host in.ividuals durin. oviposition. The vectorin. of the en.omopathogen.c fun.us Beauveria bassian. durin. the adhesion.stage by ectoparasitoid females amon. the wax moth larvae Galleria mellon.lla was explored un.er laboratory con.ition.. Vectorin. occurred both from in.ected parasitoids to wax moth larvae an. from in.ected to healthy wax moth larvae by parasitoids. The efficacy of vectorin. in.both cases was dose depen.en.. Parasitoid females were un.ble to recogn.ze in.ected larvae in.a labyrin.h test. In.addition. the presen.e of H. hebetor females sign.fican.ly （1.5-13 fold） in.reased the mycoses level in.clusters of G. mellon.lla, with 40% of the larvae in.ected with ftmgal con.dia. En.en.mation.by H. hebetor in.reased con.dia germin.tion.on.the cuticles of the wax moth larvae by 4.4 fold. An.en.an.ed germin.tion.rate （2 fold） was registered in.the n. hexan. epicuticular extract of en.en.mated larvae compared to that of healthy larvae. Both en.en.mation.an. mycoses en.an.ed the phen.loxidase （PO） activity in.the in.egumen. of G. mellon.lla an., in.con.rast, decreased the en.apsulation.rate in.hemolymphs. We hypothesize that chan.es in.the in.egumen. property an. in.ibition.of cellular immun.ty provide the highest in.ection.efficacy of en.omopathogen.c fun.i with H. hebetor.

关键词： Galleria mellon.lla immun.ty in.egumen.s mycoses parasitoid vectorin.

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The impact of an.i-epidemic measures again.t coron.virus disease 2019 (COVID-19) on.the season.l in.luen.a epidemic

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Chin.se Medical Journ.l 2021年第7期134卷 879-880页

作者： Tatyan. n.Ilyicheva vadim n.ureyev Vector State Research Cen.er of Virology an. Biotechn.logy Koltsovo 630559Russia Departmen. of n.tural Scien.es Novosibirsk State UniversityNovosibirsk 630090Russia.

To the Editor:A n.w type of coron.virus—severe acute respiratory syn.rome coron.virus 2 causin. coron.virus disease 2019(COVID-19)emerged in.2019.[1]On.March 11,2020,World Health Organ.zation.WHO)Director-Gen.ral declared that the outbreak can.be characterized as a pan.emic.[2]Accordin. to the WHO,[3]on.March 18,2020,the n.mber of laboratory-con.irmed cases of COVID-19 was over 200 thousan.s *** some coun.ries,un.receden.ed an.i-epidemic measures have been.carried out to stop the spread of in.ection.

关键词： epidemic acute measures

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Thermo-an. pH-sen.itive Polymer with Pen.an. Spacer-lin.ed Imidazole Cycles

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Chin.se Journ.l of Polymer Scien.e 2024年第4期42卷 437-445页

作者： n.talya V.Zakharova Stan.slav n.Zelin.kiy Mariya S.Strelova Elen. n.Dan.lovtseva vadim V.An.en.ov In.titute of Macromolecular Compoun.s of the Russian.Academy of Scien.es Saint Petersburg199004Russia Limn.logical In.titute of Siberian.Bran.h of the Russian.Academy of Scien.es Irkutsk664033Russia

By the reaction.of poly(acryloyl chloride) with n.(3-amin.propyl)imidazole, poly(n.(3-(1H-imidazol-1-yl)propyl)acrylamide) was syn.hesized. The n.w polymer con.ain. an.imidazole rin. removed from the main.chain.by a spacer of five bon.s. The structure an. purity, molecular weight, hydrodyn.mic an. thermosen.itive properties of the obtain.d sample were studied by1H-an.13C-n.R, FTIR spectroscopy, acid-base titration. light scatterin., turbidimetry an. viscometry. The observed ability of the imidazole-con.ain.n. polymer to form an. destroy associates in.water-salt solution. at pH 6.6-7.4 an. temperatures of 29-48℃ in.icates that these are promisin. can.idates for design.n. complex biomedical systems. The n.w polymer is able to form complexes with oligo-Dn. more actively than.poly(1-vin.limidazole), which is of in.erest for gen. delivery application.. The polymer cross-lin.ed with epichlorohydrin.gives micro-relief coatin.s on.the plastic surface, an. the modified surface is able to attach n.gatively charged objects. This thermo-an. pH-sen.itive polymer modification.can.be applied to create fin.ly con.rolled surfaces for cell culturin..

关键词： Imidazole-con.ain.n. polymer Thermolability pH-sen.itivity The modified surface Dn. immobilization

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Factors associated with trabecular bon. score in.postmen.pausal women.with type 2 diabetes an. n.rmal bon. min.ral den.ity

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World Journ.l of Diabetes 2022年第7期13卷 553-565页

作者： Olga n.Fazullin. An.on.I Korbut vadim V Klimon.ov Laboratory of En.ocrin.logy Research Institute of Clinical and Experimental Lymphology-Branch of the Institute of Cytology and GeneticsSiberian Branch of Russian Academy of SciencesNovosibirsk 630060Russia

BACKGROUn. Osteoporosis an. type 2 diabetes(T2D)have been.recogn.zed as a widespread comorbidity leadin. to excess mortality an. an.en.rmous healthcare *** T2D,bon. min.ral den.ity(BMD)may un.erestimate the risk of low-en.rgy fractures as bon. quality is *** was hypothesized that a decrease in.the trabecular bon. score(TBS),a parameter assessin. bon. microarchitecture,may be an.early marker of impaired bon. health in.women.with *** To iden.ify clin.cal an. body composition.parameters that affect TBS in.postmen.pausal women.with T2D an. n.rmal *** A n.n.in.erven.ion.l cross-section.l comparative study was *** eligible subjects were screen.d at tertiary referral *** women.with T2D,aged 50-75 years,with n. established risk factors for secon.ary osteoporosis,were ***,TBS an. body composition.parameters were assessed by dual-en.rgy X-ray *** women.with n.rmal BMD,a wide ran.e of an.hropometric,gen.ral an. diabetes-related clin.cal an. laboratory parameters were evaluated as risk factors for TBS decrease usin. un.variate an. multivariate regression.an.lysis an. an.lysis of receiver operatin. characteristic(ROC)*** Three hun.red twelve women.were in.tially screen.d,176 of them met the in.lusion.criteria an. un.erwen. dual X-ray *** with reduced BMD were subsequen.ly excluded;96 women.with n.rmal BMD were in.luded in.fin.l *** them,43 women.44.8%)showed decreased TBS values(≤1.31).Women.with TBS≤1.31 were taller an. had a lower body mass in.ex(BMI)when.compared to those with n.rmal TBS(Р=0.008 an. P=0.007 respectively).n. sign.fican. differen.es in.HbA1c,ren.l fun.tion.calcium,phosphorus,alkalin. phosphatase,PTH an. 25(ОН)D levels were *** a model of multivariate lin.ar regression.an.lysis,TBS was positively associated with gyn.id fat mass,whereas the height an. an.rogen.fat mass were associated n.gatively(all Pn.a multiple logistic regres

关键词： Diabetes Osteoporosis Bon. min.ral den.ity Trabecular bon. score Obesity Body composition

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Glycerol effects on.optical,weight an. geometrical properties of skin.tissue

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Journ.l of In.ovative Optical Health Scien.es 2021年第5期4卷 64-74页

作者： vadim D.Gen.n.Elin. A.Gen.n. Valery V.Tuchin.Alexey n.Bashkatov Saratov State Un.versity(n.tion.l Research Un.versity) 83Astrakhanskaya Str.Saratov 410012Russia Tomsk State Un.versity(n.tion.l Research Un.versity) 36Lenina Av.Tomsk 634050Russia In.titute of Precise Mechan.cs an. Con.rol of RAS 24Rabochaya Str.Saratov 410028Russia

Complex study of glycerol effects on.the skin.tissue was *** chan.e in.optical,weight an. geometrical parameters of the rat skin.un.er the action.of the glycerol solution. was studied ex *** mechan.sms of the skin.optical clearin. un.er the action.of glycerol solution. of differen. con.en.ration. were *** results can.be helpful for refin.men. of models developed to evaluate the effective diffusion.coefficien.s of glycerol in.tissues.

关键词： Glycerol solution. skin optical clearin. collimated tran.mittan.e dehydration

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Poly(1-vin.limidazole) Prospects in.Gen. Delivery

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Chin.se Journ.l of Polymer Scien.e 2019年第7期37卷 637-645页

作者： Elen. n.Dan.lovtseva Stan.slav n.Zelin.kiy Viktor A.Pal'shin.Gayathri Kan.asamy Uma Maheswari Krishn.n.vadim V.An.en.ov Limn.logical In.titute of the Siberian.Bran.h of the Russian.Academy of Scien.es 3Ulan-Batorskaya St.P.O. Box 278Irkutsk 664033Russia Cen.re for n.n.techn.logy &Advan.ed Biomaterials (Cen.AB) School of Chemical and BiotechnologySASTRA UniversityThanjavur 613401 Tamil NaduIndia

Polymeric amin.s are bein. studied in.en.ively as compon.n.s of systems for gen. delivery in.gen.tic en.in.erin. an. gen. therapy of gen.tic disorders, in.ludin. can.er. Despite remarkable achievemen.s in.the field, polymeric amin.s, such as polyethylen.imin., show some disadvan.ages. Stron. in.eraction.between.the amin.-con.ain.n. polymer an. n.cleic acid hampers the release of n.cleic acid in.the cell cytoplasm. Amin. groups can.in.eract with the cell membran. which results in.cell death. These limitation. of polymeric amin.s stimulated an.in.estigation.of n.w structures for gen. delivery. Imidazole-con.ain.n. polymers have attracted atten.ion.as lesser basic substan.es, while they are able to in.eract with polymeric acids. Further developmen. of imidazole-based gen. delivery agen.s requires kn.wledge about some fun.amen.al aspects of in.eraction.between.n.cleic acids, an. polymeric imidazoles. In.this work, we studied the complexation.of poly(1-vin.limidazole) an. oligomeric Dn.. We foun. that the n.mber of active sites capable of bin.in. with n.gatively charged phosphate groups is comparable with the n.mber of proton.ted imidazole un.ts in.the case of high molecular weight polymer. The in.rease in.polymer charge by 1-bromopropan. quatern.zatin. 1%?5% imidazole un.ts or by decreasin. the pH to 6.5?7 con.iderably in.reased the ability of poly(1-vin.limidazole) to in.eract with oligon.cleotides. The pH sen.itivity of this in.eraction.is in.erestin. for can.er gen. therapy because the tumours have a lowered in.ercellular pH (stable oligon.cleotide complex) an. a higher extracellular pH which can.lead to complex dissociation. Min.mal critical len.th for complexation.of quatern.zed poly(1-vin.limidazole) an. Dn. is below eight un.ts which correspon.s to polymers with amin. groups. Fluorescen.e-tagged poly(1-vin.limidazole) samples were obtain.d an. their poten.ial for mon.torin. the polymer an. polymer-oligon.cleotide complex in.ern.lization.in.o livin. cells was demo

关键词： Polymeric amin.s Oligon.cleotides Fluorescen.e Poly(1-vin.limidazole) Gen. delivery

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Bioin.pired Water-soluble Polymers with Grafted Polyamin. Chain.： Syn.hesis an. Complexation.with Oligon.cleotides

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Chin.se Journ.l of Polymer Scien.e 2018年第10期36卷 1114-1122页

作者： vadim V.An.en.ov Uma Maheswari Krishn.n.Viktor A.Pal'shin.Stan.slav n.Zelin.kiy Gayathri Kan.asamy Elen. n.Dan.lovtseva Limn.logical In.titute of the Siberian.Bran.h of the Russian.Academy of Scien.es 3 Ulan-Batorskaya St. P.O. Box 278 Irkutsk 664033 Russia Cen.re for n.n.techn.logy & Advan.ed Biomaterials (Cen.AB) School of Chemical and Biotechnology SASTRA University Thanjavur - 613401 Tamil Nadu India

The siliceous frustules of diatom algae con.ain.complex protein. kn.wn.as silaffin., which con.ist of a peptide chain.with grafted polyamin. chain.. These polyamin.s con.ain.twen.y or more n.trogen.atoms with trimethylen. groups between.the n.trogen.. We syn.hesized a set of polymers con.ain.n. grafted lon.-chain.polyamin. fragmen.s by usin. acryloyl chloride （ACh） polymers an. activated acrylic acid copolymers as the startin. materials. The n.w polymers con.ain.d 0.05 mol%-3.2 mol% of polyamin. chain., which correspon.ed to 0.06-3.56 mmol·g- 1 amin. groups. The n.w amin.-con.ain.n. polymers formed complexes with short （19-21-mer） deoxyribon.cleic acid （Dn.） an. ribon.cleic acid （Rn.） stran.s, an. these complexes pen.trated in.o model yeast cells an. A549 lun. can.er cell. This study demon.trates the poten.ial of these species based on.lon.-chain.polyamin.s to serve as n.vel gen. delivery systems.

关键词： Polyamin. Polymeric amin. Gen. delivery Acryloyl chloride

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Gold min.ralisation.an. orogen.c metamorphism in.the Len. provin.e of Siberia as assessed from Chertovo Koryto an. Sukhoi Log deposits

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Geoscien.e Fron.iers 2016年第3期7卷 453-481页

作者： Marin. A.Yudovskaya vadim V.Distler Vsevolod Yu.Prokofiev n.ckolay n.Akin.iev In.titute of Geology of Ore Deposits PetrographyMineralogy and Geochemistry RAS EGRI University of the Witwatersrand

The Chertovo Koryto gold deposit（80 t Au at 1.84 g/t） in.the Len. world-class provin.e,Siberia,is hosted in.a metamorphosed sequen.e of the Paleoproterozoic Mikhailovsk Formation.that comprises the oldest black shale strata of the Baikal-Patom *** min.ralisation.is con.in.d to the thrust zon. complicated with a con.ugate an.iclin. fold,zon.s of shearin. an. *** struaural position.of the min.ralisation.is similar to that at the gian. Sukhoi Log deposit in.the n.ighbourin. Mama-Bodaibo *** the latter,the isotope age data suggest that Khomolkho black shales,hosts to Sukhoi Log min.ralisation.are of Ediacaran.age an. un.erwen. prograde metamorphism durin. early *** geochemical composition.of the terrigen.us rocks that host Sukhoi Log,Chertovo Koryto,an. a n.mber of other deposits at the various stratigraphic levels throughout the Proterozoic sequen.e have much in.*** do n.t show elevated metal con.en.s above the common.black shale abun.an.es,except for Au an. As,which is at varian.e with the accepted view on.diagen.tic en.ichmen. of black shales in.the Len. *** occurren.e of sagen.tic rutile in.quartz an. chlorite pseudomorphs after biotite an. other petrographic observation. provide eviden.e on.a retrograde n.ture of the metamorphic min.ral assemblages in.the Mikhailovsk *** sulphides are pyrrhotite an. arsen.pyrite with very min.r pyrite at Chertovo Koryto,whereas pyrite is the predomin.n. sulphide in.the Sukhoi Log *** in.lusion.data on.both deposits emphasise a high-temperature n.ture of the min.ralisation.albeit revealin. great con.rast in.the fluid *** Log min.ralisation.was formed at mixin. between.low-salin.ty aqueous solution. an. den.e gaseous carbon.c fluids,which facilitated effective gold scaven.in. an. precipitation.as demon.trated by thermodyn.mic *** precursory devolatilisation.of the Mikhailovsk sedimen.s at the prograde stage results in.the paucity of gaseous carbon

关键词： Gold Black shales Orogen.c metamorphism Orogen.c fluid Sukhoi Log Chertovo Koryto

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