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冶金分析 2009年第8期29卷 1-6页

作者： PISONErO J valledor r NELIS Th BOrDEL N MEDEL A Sanz 奥维耶多大学物理系法国科学研究中心奥维耶多大学物理分析化学系

设计了一种新的实验设备用于研究发射光谱的射频辉光中原子和离子的空间分布。通过测量沿等离子体羽流的不同原子和离子的发射强度来研究发生在等离子体内部的激发/离子化过程。该报导对辉光源与质谱联用的优化有较大意义,如离子提取的... 详细信息

设计了一种新的实验设备用于研究发射光谱的射频辉光中原子和离子的空间分布。通过测量沿等离子体羽流的不同原子和离子的发射强度来研究发生在等离子体内部的激发/离子化过程。该报导对辉光源与质谱联用的优化有较大意义,如离子提取的最佳位置。初步研究了连续和脉冲射频辉光光谱中等离子体的空间解析,尤其对影响发射信号的输入功率、压强及占空比等参数进行了评价。

关键词：射频辉光发射光谱空间解析

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Proteomic Analysis through Adventitious rooting of Pinus radiata Stem Cuttings with Different rooting Capabilities

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American Journal of Plant Sciences 2016年第14期7卷 1888-1904页

作者： Carolina Álvarez Luis valledor Patricia Sáez Manuel Sánchez-Olate Darcy ríos Centro Tecnológico de la Planta Forestal (CTPF) Instituto Forestal (INFOR) Sede Bío-Bío Chile Laboratorio de Cultivo de Tejidos Vegetales Facultad de Ciencias Forestales y Centro de Biotecnología Universidad de Concepción Concepción Chile Laboratories of Adaption Biotechnologies Global Change Research Centre Academy of Sciences of the Czech Republic Brno Czech Republic Plant Physiology Epiphysage Research Group B.O.S. Department Faculty of Biology University of Oviedo Oviedo Spain

In forest production systems, vegetative propagation of elite clones through adventitious rooting is a common practice. In Chile, adventitious rooting is the main methodology for vegetative reproduction of Pinus radiata. However, the capability of produce adventitious roots in gymnosperms decreases with aging. While it is true that some efforts have been made to identify markers or/and regulators of the aging process and adventitious rooting, molecular mechanisms that regulate both processes are scarcely known, especially at protein level. This research evaluated qualitative and quantitative changes in protein accumulation during the adventitious rooting process of P. radiata stem cuttings, with different rooting capabilities. Beside, an analysis of morpho-anatomical changes was performed in stem cuttings with high and low rooting capabilities, during the adventitious rooting process. It was observed that juvenile 1-year-old stem cuttings rooted in a 100%, while aged stem cuttings (3-year-old) presented only a 20% of rooting. According to the results of differential protein accumulation, univariate and multivariate analysis indicated that in total, 114 and 89 proteins were differentially accumulated in juvenile and aged cuttings, respectively. Also, identification of such proteins showed the presence of proteins related to cell wall organization and the presence of a protein related with proper distribution of auxin PIN transporter, both key in the new meristem formation process during adventitious rooting.

关键词： Pinus radiata Adventitious rooting Protein Accumulation Aging

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Changes in Gene Expression in Needles and Stems of Pinus radiata rootstock Plants of Different Ontogenic Age

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American Journal of Plant Sciences 2016年第8期7卷 1205-1216页

作者： Carolina Alvarez Luis valledor Patricia Sáez rodrigo Hasbún Manuel Sánchez-Olate María Jesús Cañal Darcy ríos Laboratorio de Cultivo de Tejidos Vegetales Facultad de Ciencias Forestales y Centro de Biotecnología Universidad de Concepción Concepción Chile Centro Tecnológico de la Planta Forestal (CTPF) Instituto Forestal (INFOR) Sede Bío-Bío Chile Laboratories of Adaption Biotechnologies Global Change Research Centre Academy of Sciences of the Czech Republic Brno Czech Republic Plant Physiology Epiphysage Research Group Biology of Organisms and Systems Department Faculty of Biology University of Oviedo Oviedo Spain

A major problem in forest clonal productivity is the loss of morphogenetic capability with the increasing age of plants. However, despite of the importance of loss of morphogenetic competence, very little research has been done about the underlying mechanisms involved in this process. For this reason, a gene expression analysis using dot blot technique was performed in needles and stems of 1- and 3-year old Pinus radiata rootstock plants with a proved decrease in morphogenetic competence. Needles of one year old rootstock plants showed a higher number of up-regulated in genes mainly corresponding to photosynthesis and protein synthesis, degradation and modification, reflecting a higher number of active pathways in younger hedges, contrary to the older ones. Gene expression profiles found in stems are in agreement with those found in needles, indicating more active pathways in younger rootstock plants than in older ones. Several transcripts regulating transcription and translation were up-regulated in young competent tissues. Three-year-old stems presented an increase in the expression of an ethylene response factor, involved in plant organ senescence, indicating that pathways involved in senescence and ageing might inhibit the adventitious root formation, as in the older cuttings.

关键词： Plant Ageing Gene Expression Morphogenetic Competence Pinus radiate

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