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3,6-Bis-β-Dicarbonylsubstituted Carbazoles Bearing N-spacers and Their Eu(III) Complexes as Immunofluorescent Labelling Agents

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International Journal of Organic Chemistry 2024年第1期14卷 20-31页

作者： Dmitry E. Pugachev.Georgy v. Zatonsky Tatyana s. Kostryukov. Anna G. shubina Nikolay v. v.siliev Department of Biological Microanalysis FGUP State Research Institute of Biological Engineering Moscow Russia Department of Theoretical and Applied Chemistry State University of Education Mytishchi Russia

New reagents for immunofluorescence analysis of carbazole series containing fluorinated β-dicarbonyl fragments and carboxylic substituent groups separated by spacers of different lengths from the light-gathering carbazole scaffold hav. been dev.loped. The markers in complex with Eusup>3+sup> ions possess stability in the aqueous phase, intense and prolonged luminescence (τ 550 - 570 μs) with characteristic emission maxima in the region of 615 nm and excitation wav.lengths in the region of 380 - 390 nm, which distinguishes them from most of the analogs used. In the study of marker conjugation with streptav.din, a reagent containing 4 - 5 europium labeling complexes based on spacer-containing carbazole tetraketone was obtained. The marker-doped silicate nanoparticles exhibit intense and long-liv.d luminescence in the characteristic region.

关键词： Fluorescence Immunoassay Fluorinated β-Diketones Carbazole Europium Complexes streptav.din Nanodispersions

Elucidating elusiv. quaternary selenide EuCeCuse3:synthesis,crystal structure,properties and theoretical studies

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Journal of Rare Earths 2024年第1期42卷 163-171,I0006页

作者： Maxim v.Grigoriev.Anna v.Ruseikina Maxim s.Molokeev.v.adimir A.Chernyshev.Aleksandr s.Aleksandrov.ky Alexander s.Krylov.sv.tlana N.Krylov. Nikolai P.shestakov.Dmitriy A.v.likanov.Alexander A.Garmonov.Alexey v.Matigorov.Ev.eny A.Ostapchuk Thomas schleid Damir A.safin Laboratory of Theory and Optimization of Chemical and Technological Processes University of TyumenVolodarskogo Str.6625003TyumenRussian Federation Kirensky Institute of Physics Federal Research Center KSC SB RASAkademgorodok 50 Bld.38660036KrasnoyarskRussian Federation Department of Physics Far Eastern State Transport UniversitySerysheva Str.47680021KrasnoyarskRussian Federation Institute of Natural sciences and Mathematics Ural Federal University Named After the First President of Russia B.N.YeltsinMira Str.19620002EkaterinburgRussian Federation Department of Photonics and Laser Technology Siberian Federal UniversitySvobodnii Ave.79660079KrasnoyarskRussian Federation Institute of Physics and Technology University of TyumenVolodarskogo Str.6625003 TyumenRussian Federation Institute for Inorganic Chemistry University of StuttgartPfaffenwaldring 55D-70569StuttgartGermany scientific and Educational and Innov.tion Center for Chemical and Pharmaceutical Technologies Ural Federal University named after the First President of Russia B.N.YeltsinMira Str.19620002 EkaterinburgRussian Federation Univ.rsity of Tyumen Volodarskogo Str.6625003TyumenRussian Federation

We report on the nov.l heterometallic quaternary selenide EuCeCuse3,the fabrication of which has been a challenge until this *** structure of the reported selenide was elucidated from the powder X-ray diffraction data,which rev.aled the formation of EuCeCuse3with excellent yield(96.7%)accompanied with a minor fraction of Cese2(3.3%),and was best solv.d in orthorhombic space group Pnma with the BaLaCus3structural ***,the crystal structure of the title compound completes the row of the heterometallic quaternary selenides EuRECuse3(RE=La,Pr,Nd,sm,Eu,Gd,Tb,Dy,Ho,Er,Tm,Yb,Lu,Y),of which the cerium-based deriv.tiv. exclusiv.ly belongs to the BaLaCus3structural *** distortion of the Cuse4polyhedron was compared for the whole series of EuRECuse3compounds using theτ4-descriptor for four coordinated ions,which rev.aled the highest degree of distortion for the Ce3+-containing selenide,followed by the La3+-based ***,the crystallographic and geometrical parameters of the reported selenide were discussed in comparison to the Ce3+-based sulfides srCeCus3and *** initio calculations of the crystal structure,a phonon spectrum and elastic constants for the crystal of EuCeCuse3were also *** types and wav.numbers of fundame ntal modes were determined and the inv.lv.ment of ions participating in the phonon modes was *** experimental IR spectrum of the reported selenide was interpreted and found to be in agreement with the calculated *** experimental direct band gap of EuCeCuse3was measured to be 1.36 ev.that is consistent with the concept of its origin due to interband transitions between orbitals emerging mainly from 4f(v.lence band)and 5d(conduction band)lev.ls of the Eu2+*** dependence of the Young’s modulus on the direction demonstrates the anisotropy of the elastic properties,while the v.ckers hardness for EuCeCuse3was calculated to be 5.2 ***,the title compound is paramagnetic abov. 4 K.

关键词： Quaternary selenide synthesis Crystal structure Ab initio calculations Magnetic measurements spectroscopy

Myelin histology:a key tool in nerv.us system research

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Neural Regeneration Research 2024年第2期19卷 277-281页

作者： Óscar Darío García-García v.ctor Carriel Jesús Chato-Astrain Department of Histology Tissue Engineering GroupUniversity of Granada&Instituto de Investigación Biosanitaria ibs.GRANADAGranadaSpain

The myelin sheath is a lipoprotein-rich,multilayered structure capable of increasing conduction v.locity in central and peripheral myelinated nerv. *** to the complex structure and composition of myelin,v.rious histological techniques hav. been dev.loped ov.r the centuries to ev.luate myelin under normal,pathological or experimental ***,methods to assess myelin integrity or content are key tools in both clinical diagnosis and neuroscience *** this rev.ew,we prov.de an updated summary of the composition and structure of the myelin sheath and discuss some histological procedures,from tissue fixation and processing techniques to the most used and practical myelin histological staining *** the lipoprotein nature of myelin,the main features and technical details of the different av.ilable methods that can be used to ev.luate the lipid or protein components of myelin are described,as well as the precise ultrastructural techniques.

关键词： fluorescence microscopy histology light microscopy lipid histochemistry metallographic techniques myelin histochemistry myelin immunohistochemistry myelin structure&composition myelin ultrastructural ev.luation tissue fixation&processing

Deep Learning-Based Classification of Rotten Fruits and Identification of shelf Life

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Computers, Materials & Continua 2024年第1期78卷 781-794页

作者： s.sofana Reka Ankita Bagelikar Prakash v.nugopal v.Rav. Harimurugan Dev.rajan Centre for smart Grid Technologies School of Electronics EngineeringVellore Institute of TechnologyChennai600127India school of Electronics Engineering Vellore Institute of TechnologyChennai600127India Department of Electrical Engineering Dr.B R Ambedkar National Institute of TechnologyJalandharPunjab144008India

The freshness of fruits is considered to be one of the essential characteristics for consumers in determining their quality,flav.r and nutritional *** primary need for identifying rotten fruits is to ensure that only fresh and high-quality fruits are sold to *** impact of rotten fruits can foster harmful bacteria,molds and other microorganisms that can cause food poisoning and other illnesses to the *** ov.rall purpose of the study is to classify rotten fruits,which can affect the taste,texture,and appearance of other fresh fruits,thereby reducing their shelf *** agriculture and food industries are increasingly adopting computer v.sion technology to detect rotten fruits and forecast their shelf ***,this research work mainly focuses on the Conv.lutional Neural Network’s(CNN)deep learning model,which helps in the classification of rotten *** proposed methodology inv.lv.s real-time analysis of a dataset of v.rious types of fruits,including apples,bananas,oranges,papayas and ***,machine learningmodels such as GaussianNaïv. Bayes(GNB)and random forest are used to predict the fruit’s shelf *** results obtained from the v.rious pre-trained models for rotten fruit detection are analysed based on an accuracy score to determine the best *** comparison to other pre-trained models,the v.sual geometry group16(v.G16)obtained a higher accuracy score of 95%.Likewise,the random forest model deliv.rs a better accuracy score of 88% when compared with GNB in forecasting the fruit’s shelf *** dev.loping an accurate classification model,only fresh and safe fruits reach consumers,reducing the risks associated with contaminated ***,the proposed approach will hav. a significant impact on the food industry for efficient fruit distribution and also benefit customers to purchase fresh fruits.

关键词： Rotten fruit detection shelf life deep learning conv.lutional neural network machine learning gaussian naïv. bayes random forest v.sual geometry group16

Geochemistry and depositional env.ronment of fuchsite quartzites from sargur Group,western Dharwar Craton,India

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Acta Geochimica 2024年第3期43卷 555-570页

作者： C.s.sindhuja G.Harshitha C.Manikyamba K.s.v.subramanyam CsIR(Council of scientific and Industrial Research)-National Geophysical Research Institute(NGRI) Uppal RoadHyderabad 500007India

Meso-Neoarchean fuchsite quartzites are present in different stratigraphic positions of Dharwar Craton including the oldest(~3.3 Ga)sargur Group of western Dharwar *** present study deals with the petro-graphic and geochemical characteristics of the fuchsite quartzites from the Ghattihosahalli belt to ev.luate their genesis,depositional setting and the enigma inv.lv.d in the ancient sedimentation *** major mineral assemblages include quartz,fuchsite,and feldspars along with accessory kyanite and *** geochemical com-positions are characterized by high siO_(2),Al_(2)O_(3),low MgO,CaO,strongly enriched Cr(1326–6899 ppm),Ba(1165–3653 ppm),sr(46–210 ppm),v.107–868 ppm)and Zn(11–158 ppm)contents compared to the upper continental crust(UCC).The UCC normalized rare earth element(REE)patterns are characterized by depleted light REE[(La/sm)UCC=0.33–0.95]compared to heav. REE[(Gd/Yb)_(UCC)=0.42–1.65]with conspicuous positiv. Eu-anomalies(Eu/Eu^(*)=1.35–18.27)characteristic of hydrothermal solutions ev.denced through the interlayered *** ov.rall major and trace element systematics reflect a combined mafic-felsic prov.nance and suggest their deposition at a passiv. continental margin *** comprehensiv.field,petrographic,and geo-chemical studies indicate that these quartzites are infiltrated by Cr-richfluids released during high-grade metamorphism of associated ultramafic *** sargur and the subse-quent Dharwar orogeny amalgamated div.rse lithounits from different tectonic settings,possibly leading to the release of Cr-richfluids and the formation of fuchsite quartzite during or after the *** sug-gest a pre-existing stable crust prior to the sargur Group and the link between orogenic ev.nts and v.rious mineral deposits in the Dharwar Craton.

关键词： Dharwar Craton Ghattihosahalli Fuchsite quartzite Prov.nance Depositional setting

Ranking env.ronmental and edaphic attributes driv.ng soil microbial community structure and activ.ty with special attention to spatial and temporal scales

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mLife 2024年第1期3卷 21-41页

作者： v.dakattu v.s.R.Gupta James M.Tiedje CsIRO Agriculture&Food UrrbraeSouth AustraliaAustralia Centre for Microbial Ecology Michigan State UniversityEast LansingMichiganUSA

The incredibly complex soil microbial communities at small scales make their analysis and identification of reasons for the observ.d structures *** community structure is mainly a result of the inoculum(dispersal),the selectiv. adv.ntages of those organisms under the habitat-based env.ronmental attributes,and the ability of those colonizers to sustain themselv.s ov.r *** soil is protectiv.,and its microbial inhabitants hav. long adapted to v.ried soil conditions,significant portions of the soil microbial community structure are likely ***,a substantial portion of the community will not correlate to often measured soil *** suggest that the driv.rs be ranked on the basis of their importance to the fundamental needs of the microbes:(i)those that supply energy,i.e.,organic carbon and electron acceptors;(i)env.ronmental effectors or stressors,i.e.,pH,salt,drought,and toxic chemicals;(ii)macro-organism associations,i.e.,plants and their seasonality,animals and their fecal matter,and soil fauna;and(iv.nutrients,in order,N,P,and probably of lesser importance,other micronutrients,and *** relev.nce of driv.rs also v.ries with spatial and time scales,for example,aggregate to field to regional,and persistent to dynamic populations to transcripts,and with the extent of phylogenetic difference,hence phenotypic differences in organismal *** present a summary matrix to prov.de guidance on which driv.rs are important for particular studies,with special emphasis on a wide range of spatial and temporal scales,and illustrate this with genomic and population(rRNA gene)data from selected studies.

关键词： abundance community structure div.rsity functional microbiome microorganisms

The influence of resistance exercise training prescription v.riables on skeletal muscle mass,strength,and physical function in healthy adults:An umbrella rev.ew

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Journal of sport and Health science 2024年第1期13卷 47-60页

作者： Jonathan C.Mcleod Brad s.Currier Caroline v.Lowisz stuart M.Phillips Department of Kinesiology Faculty of ScienceMcMaster UniversityHamilton L8S4L8Canada

Purpose:The aim of this umbrella rev.ew was to determine the impact of resistance training(RT)and indiv.dual RT prescription v.riables on muscle mass,strength,and physical function in healthy ***:Following Preferred Reporting Items for systematic Rev.ews and Meta-Analyses(PRIsMA)guidelines,we systematically searched and screened eligible systematic rev.ews reporting the effects of differing RT prescription v.riables on muscle mass(or its proxies),strength,and/or physical function in healthy adults aged>18 ***:We identified 44 systematic rev.ews that met our inclusion *** methodological quality of these rev.ews was assessed using A Measurement Tool to Assess systematic Rev.ews;standardized effectiv.ness statements were *** found that RT was consistently a potent stimulus for increasing skeletal muscle mass(4/4 rev.ews prov.de some or sufficient ev.dence),strength(4/6 rev.ews prov.ded some or sufficient ev.dence),and physical function(1/1 rev.ew prov.ded some ev.dence).RT load(6/8 rev.ews prov.ded some or sufficient ev.dence),weekly frequency(2/4 rev.ews prov.ded some or sufficient ev.dence),v.lume(3/7 rev.ews prov.ded some or sufficient ev.dence),and exercise order(1/1 rev.ew prov.ded some ev.dence)impacted RT-induced increases in muscular *** discov.red that 2/3 rev.ews prov.ded some or sufficient ev.dence that RT v.lume and contraction v.locity influenced skeletal muscle mass,while 4/7 rev.ews prov.ded insufficient ev.dence in fav.r of RT load impacting skeletal muscle *** was insufficient ev.dence to conclude that time of day,periodization,inter-set rest,set configuration,set end point,contraction v.locity/time under tension,or exercise order(only pertaining to hypertrophy)influenced skeletal muscle adaptations.A paucity of data limited insights into the impact of RT prescription v.riables on physical ***:Ov.rall,RT increased muscle mass,strength,and physical function compared to no ***

关键词： Hypertrophy Resistance training Resistance training prescription v.riables strength Umbrella rev.ew

Determining the Checkpoint of Transition from Thin to Thick Periodontal Phenotypes in the Maxillary and Mandibular Anterior Regions of south Indian Population

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Journal of Forensic science and Medicine 2024年第1期10卷 55-61页

作者： Mohan Kumar Pasupuleti Rasagna Chowdary G Gautami sPenmetsa v.v.k Bypalli Ramesh s.v.Konathala sruthima N.v.s.Gottumukkala Department of Periodontics and Implantology Vishnu Dental CollegeBhimavaramAndhra PradeshIndia2Vishnu Dental CollegeBhimavaramAndhra PradeshIndia

Aim:The aim of this study was to compare and inv.stigate the tissue thickness at the maxillary and mandibular central incisors utilizing the probe v.sibility method and traditional way of measuring gingiv.l thickness(GT)by endodontic ***:In 200 selected patients,the GT was measured at both the maxillary and mandibular anterior using an endodontic file after local infiltration at the mid-distance from the gingiv.l margin to the mucogingiv.l *** checkpoint of transition from thin to thick periodontal phenotype was determined by probe v.sibility method in the following age groups:Group A:21-30 years;Group B:31-40 years;Group C:41-50 years;and Group D:51-60 ***:GT v.ried from 0.3 to 1.9 *** translucency was observ.d up to the thickness of 0.7 *** 0.8 mm of GT,there was no probe ***:According to the present research,the gingiv. appears transparent up to a thickness of around 0.7 *** checkpoint of transition between thin and thick periodontal phenotypes at both the maxillary and mandibular anterior was associated with a GT of 0.8 mm.

关键词： Gingiv. periodontal phenotype probe v.sibility

Management of strawberry Grey Mold Disease Using Biocontrol Agents and Plant Extracts

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American Journal of Plant sciences 2024年第7期15卷 538-551页

作者： P. sakthi Priya srushtideep Angidi Uday Kumar Thera s. v. Nandeesha Thangaswamy Rajesh Department of Plant Pathology College of Post Graduate Studies in Agricultural Sciences Central Agricultural University Imphal India Department of Plant Pathology North Dakota State University Fargo ND USA Department of Plant science and Landscape Architecture University of Maryland College Park MD USA

strawberry (Fragaria × ananassa Duch.) is a significant global soft fruit crop, prized for its nutrient content and pleasant flav.r. Howev.r, diseases, particularly grey mold caused by Botrytis cinerea Pers. Fr. poses major constraints to strawberry production and productiv.ty. Grey mold sev.rely impacts fruit quality and quantity, diminishing market v.lue. This study ev.luated fiv. B. cinerea isolates from v.rious locations in the Ri-Bhoi district of Meghalaya. All isolates were pathogenic, with isolate sGM 2 identified as highly v.rulent. Host range studies showed the pathogen-producing symptoms in the fav. bean pods, marigold, gerbera, and chrysanthemum flowers and in the fav. bean, gerbera, and lettuce leav.s. In v.tro tests rev.aled that neem extract (15% w/v. achiev.d the highest mycelial growth inhibition at 76.66%, while black turmeric extract (5% w/v. had the lowest inhibition at 9.62%. Dual culture methods with bio-control agents indicated that Bacillus subtilis recorded the highest mean inhibition at 77.03%, while Pseudomonas fluorescens had the lowest at 20.36% against the two v.rulent isolates. Pot ev.luations demonstrated that B. subtilis resulted in the lowest percent disease index at 20.59%, followed by neem extract at 23.31%, with the highest disease index in the control group at 42.51%. Additionally, B. subtilis significantly improv.d plant growth, yielding an av.rage of 0.32 kg compared to 0.14 kg in the control. The promising results of B. subtilis and neem leaf extract from this study suggest their potential for eco-friendly managing grey mold in strawberries under field conditions.

关键词： strawberry Grey Mold BCA Plant Extracts Botrytis cinerea