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公猪精子的冷冻:浓缩精液和新解冻法的受精能力

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贵州畜牧兽医 1980年第2期 49-56页

作者： v·g·pursel L·A·Johnson

1971年(Crabo和Einarson,1971;graham等,1971 a,b;pursel和Johnson,1971 a,b,c)第一次报告,用冷冻公猪精液进行子宫颈输精,相继获得成功的受精。此后Crabo等(1972),Paquignon和Du Mesnil du Buisson(1973),pursel和Johnson(1972a,b),Ric... 详细信息

关键词：母猪受精率精子繁殖性能生殖细胞受精能力精液

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猪精液处理对精子获能时间的影响

国外畜牧科技 1984年第4期 16-19页

作者：遇建成 v.g.pursel

青年母猪的排卵时间,用口服 altreno-gest(17-allye-hydroxyestra-4,9,11-trien-3-one),接着注射促性腺激素的方法来控制。在注射绒毛膜促性腺激素之后的42—44小时,给母猪剖腹。精液按下述方法之一处理后用手术法把精子输入已暴露出的... 详细信息

青年母猪的排卵时间,用口服 altreno-gest(17-allye-hydroxyestra-4,9,11-trien-3-one),接着注射促性腺激素的方法来控制。在注射绒毛膜促性腺激素之后的42—44小时,给母猪剖腹。精液按下述方法之一处理后用手术法把精子输入已暴露出的输卵管内。冷冻精液融解在贝兹维尔解冻液内(F—BTS)或精清内(F—SP);未冷冻精液直接稀释在贝兹维尔解冻液内(U—BTS)或精清内(U—SP)。在输精后5和24小时收集卵子,并确定其发育程度。在5小时,F-BTS 和 U—BTS 精子使卵受精的比率(分别为45%和69%)显著高于 F-SP 和 U—SP精子(分别为5%和34%)。在两次集卵时均发现,用 F—BTS 和 U—BTS 精子受精的卵子比用 F-SP 和 U-SP 精子受精的卵发育程度更高。在输卵管输精后5小时,未冷冻精子(U-BTS 或 U-SP)的受精卵子数超过 F—BTS或 F-SP精子(P<0.005);在24小时,各种处理方法的受精卵百分率相似。结果表明,精液处理将公猪精子的获能时间减少到与精子通过子宫长度的时间相似的程度。经冷冻—融解精子的获能时间同未冷冻精子相似。

关键词：精子精液处理 SP 公猪生殖细胞精子获能 BTS

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家畜基因转移研究的进展

国外畜牧科技 1988年第3期 6-9页

作者： v.g.pursel 蔡正华

前言重组基因导入动物的技术是从1980年才开始采用的(Palmiter和Brinster,1985)。起初,包括基因调节和基础生物学问题的大多数研究,都是用小鼠进行的。把基因移植用于其它物种就需要确定外源基因对若干物种的影响,需要提供做某些生理研... 详细信息

前言重组基因导入动物的技术是从1980年才开始采用的(Palmiter和Brinster,1985)。起初,包括基因调节和基础生物学问题的大多数研究,都是用小鼠进行的。把基因移植用于其它物种就需要确定外源基因对若干物种的影响,需要提供做某些生理研究的大量血液和组织,以及查明对提高家畜生产效率和抗病力的可能性。

关键词：基因转移 gH MT 外源基因微注射家畜农畜

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转基因家畜的研究状况

草食家畜 1993年第6期 15-19页

作者： v.g.pursel 吴学清

微注射是将基因转移至家畜的主要方法。研究的目的是改良家畜的生产性状,增强家畜健康,使其生产有利于人类健康的生物药品。最初的转基因研究主要涉及编码为生长激素(gH)、生长激素释放因子(gRF)、类胰岛素生长因子(IgF-1)的基因。最近... 详细信息

微注射是将基因转移至家畜的主要方法。研究的目的是改良家畜的生产性状,增强家畜健康,使其生产有利于人类健康的生物药品。最初的转基因研究主要涉及编码为生长激素(gH)、生长激素释放因子(gRF)、类胰岛素生长因子(IgF-1)的基因。最近的研究领域是:促进家畜肌肉发育,使家畜使用细菌酶合成某些必需氨基酸,诱发特异性免疫球蛋白基因或疾病抗性基因表达,让乳腺、特定组织或特定细胞直接表达人类蛋白,生产有益于人类健康的产品。限制进展的因素是:缺乏生产性状和疾病抗性性状的可用克隆基因和对于转基因调控机理了解不充分。

关键词：转基因家畜生长药品微注射

猪浓缩冷冻精液的受胎能力和一种新的解冻操作方法

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中国畜牧兽医 1977年第4期 46-49页

作者：陈幼春 ,v.g.pursel ,L.A.Johnson

采精后2小时,将每份含6×10～9个精子的精液离心分离10分钟（300g）。去除精清,并用BF5稀释液把精子重新稀释成5毫升的混匀精液。精液在2小时内逐步冷却到5℃,再加入5毫升含2%甘油的BF5液。将精液混匀后,立即在干冰上冷冻成0.15到0.2毫... 详细信息

采精后2小时,将每份含6×10～9个精子的精液离心分离10分钟（300g）。去除精清,并用BF5稀释液把精子重新稀释成5毫升的混匀精液。精液在2小时内逐步冷却到5℃,再加入5毫升含2%甘油的BF5液。将精液混匀后,立即在干冰上冷冻成0.15到0.2毫升的颗粒,然后移入液氮保存。输精时,每次从液氮中取出10毫升的颗粒,倒入泡沫塑料制的槽形容器内,静置三分钟,随即把颗粒倾入250毫升的烧杯中,杯内装有25毫升50℃的BTS液,摇动烧杯直至颗粒融化为止。解冻的精液倒入输精瓶,再用15毫升的BTS液冲洗烧杯,洗下的液体加到输精瓶中。用一头公猪的单精或用二头公猪的混精的冷冻精液给26头小母猪输精。每头小母猪输精两次,每个剂量含6×10～9个精子。26头小母猪中有22头（85%）产生受精卵,22头的卵有87%受精并正常地发育。混精输精的受胎效果并不比单精输精优异。本研究中使用的冷冻和解冻法操作简易,贮存容量小,精液活力高;妊娠率和受精率接近新鲜精液的正常指标。

关键词：精液活力受精卵卵子卵细胞生殖细胞小母猪输精授精操作方法

An SPH Framework for Earthquake-Induced Liquefaction Hazard Assessment of geotechnical Structures

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Computer Modeling in Engineering & Sciences 2025年第1期142卷 251-277页

作者： Sourabh Mhaski g.v.Ramana Department of Civil Engineering Indian Institute of Technology DelhiNew Delhi110016India

Earthquake-induced soil liquefaction poses significant risks to the stability of geotechnical structures *** understanding of the liquefaction triggering,and the post-failure large deformation behaviour is essential for designing resilient *** present study develops a Smoothed Particle Hydrodynamics(SPH)framework for earthquake-induced liquefaction hazard assessment of geotechnical *** coupled flowdeformation behaviour of soils subjected to cyclic loading is described using the PM4Sand model implemented in a three-phase,single-layer SPH framework.A staggered discretisation scheme based on the stress particle SPH approach is adopted to minimise numerical inaccuracies caused by zero-energy modes and tensile ***,non-reflecting boundary conditions for seismic analysis of semi-infinite soil domains using the SPH method are *** numerical framework is employed for the analysis of cyclic direct simple shear test,seismic analysis of a level ground site,and liquefaction-induced failure of the Lower San Fernando *** agreement for liquefaction triggering and post-failure behaviour demonstrates that the SPH framework can be utilised to assess the effect of seismic loading on field-scale geotechnical *** present study also serves as the basis for future advancements of the SPH method for applications related to earthquake geotechnical engineering.

关键词： Earthquake seismic liquefaction stress particle PM4Sand smoothed particle hydrodynamics(SPH)

Semantic Segmentation of Lumbar vertebrae Using Meijering U-Net(MU-Net)on Spine Magnetic Resonance Images

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Computer Modeling in Engineering & Sciences 2025年第1期142卷 733-757页

作者： Lakshmi S v v Shiloah Elizabeth Darmanayagam Sunil Retmin Raj Cyril Department of Computer Science and Engineering College of Engineering GuindyAnna UniversityChennai600025India Department of Information Technology Madras Institute of TechnologyAnna UniversityChrompetChennai600044India

Lower back pain is one of the most common medical problems in the world and it is experienced by a huge percentage of people *** to its ability to produce a detailed view of the soft tissues,including the spinal cord,nerves,intervertebral discs,and vertebrae,Magnetic Resonance Imaging is thought to be the most effective method for imaging the *** semantic segmentation of vertebrae plays a major role in the diagnostic process of lumbar *** is difficult to semantically partition the vertebrae in Magnetic Resonance Images from the surrounding variety of tissues,including muscles,ligaments,and intervertebral discs.U-Net is a powerful deep-learning architecture to handle the challenges of medical image analysis tasks and achieves high segmentation *** work proposes a modified U-Net architecture namely MU-Net,consisting of the Meijering convolutional layer that incorporates the Meijering filter to perform the semantic segmentation of lumbar vertebrae L1 to L5 and sacral vertebra ***-colour mask images were generated and used as ground truth for training the *** work has been carried out on 1312 images expanded from T1-weighted mid-sagittal MRI images of 515 patients in the Lumbar Spine MRI Dataset publicly available from Mendeley *** proposed MU-Net model for the semantic segmentation of the lumbar vertebrae gives better performance with 98.79%of pixel accuracy(PA),98.66%of dice similarity coefficient(DSC),97.36%of Jaccard coefficient,and 92.55%mean Intersection over Union(mean IoU)metrics using the mentioned dataset.

关键词： Computer aided diagnosis(CAD) magnetic resonance imaging(MRI) semantic segmentation lumbar vertebrae deep learning U-Net model

Autism spectrum disorder:difficulties in diagnosis and microRNA biomarkers

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Neural Regeneration Research 2025年第10期20卷 2776-2786页

作者： Bridget Martinez Philip v.Peplow Department of Pharmacology University of Nevada-RenoRenoNVUSA Department of Medicine University of Nevada-RenoRenoNVUSA Department of Anatomy University of OtagoDunedinNew Zealand

We performed a PubMed search for microRNAs in autism spectrum disorder that could serve as diagnostic biomarkers in patients and selected 17 articles published from January 2008 to December 2023,of which 4 studies were performed with whole blood,4 with blood plasma,5 with blood serum,1 with serum neural cell adhesion molecule L1-captured extracellular vesicles,1 with blood cells,and 2 with peripheral blood mononuclear *** of the studies involved children and the study cohorts were largely *** of the studies had performed microRNA sequencing or quantitative polymerase chain reaction assays to measure microRNA *** five studies had used real-time polymerase chain reaction assay to validate microRNA expression in autism spectrum disorder subjects compared to *** microRNAs that were validated in these studies may be considered as potential candidate biomarkers for autism spectrum disorder and include miR-500a-5p,-197-5p,-424-5p,-664a-3p,-365a-3p,-619-5p,-664a-3p,-3135a,-328-3p,and-500a-5p in blood plasma and miR-151a-3p,-181b-5p,-320a,-328,-433,-489,-572,-663a,-101-3p,-106b-5p,-19b-3p,-195-5p,and-130a-3p in blood serum of children,and miR-15b-5p and-6126 in whole blood of *** important limitations were identified in the studies reviewed,and need to be taken into account in future *** studies are warranted with children and adults having different levels of autism spectrum disorder severity and consideration should be given to using animal models of autism spectrum disorder to investigate the effects of suppressing or overexpressing specific microRNAs as a novel therapy.

关键词： autism spectrum disorder biomarker blood cells blood plasma blood serum diagnosis microRNA peripheral blood mononuclear cells serum neural cell adhesion molecule L1-captured extracellular vesicles whole blood

MicroRNAs as potential diagnostic biomarkers for bipolar disorder

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Neural Regeneration Research 2025年第6期20卷 1681-1695页

Abnormal expression of microRNAs is connected to brain development and disease and could provide novel biomarkers for the diagnosis and prognosis of bipolar disorder. We performed a PubMed search for microRNA biomarkers in bipolar disorder and found 18 original research articles on studies performed with human patients and published from January 2011 to June 2023. These studies included microRNA profiling in bloodand brain-based materials. From the studies that had validated the preliminary findings,potential candidate biomarkers for bipolar disorder in adults could be miR-140-3p,-30d-5p,-330-5p,-378a-5p,-21-3p,-330-3p,-345-5p in whole blood, miR-19b-3p,-1180-3p,-125a-5p, let-7e-5p in blood plasma, and miR-7-5p,-23b-5p,-142-3p,-221-5p,-370-3p in the blood serum. Two of the studies had investigated the changes in microRNA expression of patients with bipolar disorder receiving treatment. One showed a significant increase in plasma miR-134 compared to baseline after 4 weeks of treatment which included typical antipsychotics, atypical antipsychotics, and benzodiazepines. The other study had assessed the effects of prescribed medications which included neurotransmitter receptorsite binders(drug class B) and sedatives, hypnotics, anticonvulsants, and analgesics(drug class C) on microRNA results. The combined effects of the two drug classes increased the significance of the results for miR-219 and-29c with miR-30e-3p and-526b* acquiring significance. MicroRNAs were tested to see if they could serve as biomarkers of bipolar disorder at different clinical states of mania, depression, and euthymia. One study showed that upregulation in whole blood of miR-9-5p,-29a-3p,-106a-5p,-106b-5p,-107,-125a-3p,-125b-5p and of miR-107,-125a-3p occurred in manic and euthymic patients compared to controls, respectively, and that upregulation of miR-106a-5p,-107 was found for manic compared to euthymic patients. In two other studies using blood plasma,downregulation of miR-134 was observed i

关键词： biomarker bipolar disorder blood leukocytes blood plasma blood plasma extracellular vesicles/exosomes blood serum brain tissue brain tissue extracellular vesicles/exosomes lymphoblastoid cell lines microRNA neural progenitor cells whole blood