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Characterization of Ion Contents and Metabolic Responses to Salt Stress of Different Arabidopsis AtHKT1;1 Genotypes and Their Parental Strains

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Molecular Plant 2013年第2期6卷 350-368页

作者： Camilla B. Hill Deepa Jha Antony Bacic Mark Tester ute roessner Australian Centre for Plant Functional Genomics School of Botany University of Melbourne Victoria 3010 Australia Australian Centre for Plant Functional Genomics University of Adelaide Waite Campus Glen Osmond 5064 SA Australia Metabolomics Australia School of Botany University of Melbourne Victoria 3010 Australia Bio21 Molecular Science and Biotechnology Institute University of Melbourne Victoria 3010 Australia ARC Centre of Excellence in Plant Cell Walls School of Botany University of Melbourne Victoria 3010 Australia

Plants employ several strategies to maintain cellular ion homeostasis under salinity stress, including mediat-ing ion fluxes by transmembrane transport proteins and adjusting osmotic pressure by accumulating osmolytes. The HKT （high-affinity potassium transporter） gene family comprises Na^＋ and Na^＋/K^＋ transporters in diverse plant species, with HKT1,1 as the only member in Arabidopsis thaliana. Cell-type-specific overexpression of AtHKT1;1 has been shown to prevent shoot Na^＋ overaccumulation under salinity stress. Here, we analyzed a broad range of metabolites and elements in shoots and roots of different AtHKT1;1 genotypes and their parental strains before and after salinity stress, revealing a reciprocal relationship of metabolite differences between an AtHKT1;1 knockout line （hktl;1） and the AtHKT1;1 overex- pressing lines （E2586 UASGAL4：HKT1;1 and J2731＊UASGAL4：HKT1;1）. Although levels of root sugars were increased after salt stress in both AtHKTI,1 overexpressing lines, E2586 UASGAL4：HKT1;1 showed higher accumulation of the osmopro-tectants trehalose, gentiobiose, and melibiose, whereas J2731＊UASGAL4：HKT1;1 showed higher levels of sucrose and raffinose, compared with their parental lines, respectively. In contrast, the knockout line hktl,1 showed strong increases in the levels of the tricarboxylic acid （TCA） cycle intermediates in the shoots after salt treatment. This coincided with a significant depletion of sugars, suggesting that there is an increased rate of carbon influx into the TCA cycle at a constant rate of C-efflux from the cycle, which might be needed to support plant survival during salt stress. Using correlation analysis, we identified associations between the Na^＋ content and several sugars, suggesting that regulation of sugar metabolism is important in plant responses to salinity stress.

关键词： abiotic stress salinity salt tolerance HKT sodium transporter, metabolomics enhancer trap system.

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Drought Responses of Leaf Tissues from Wheat Cultivars of Differing Drought Tolerance at the Metabolite Level

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Molecular Plant 2012年第2期5卷 418-429页

作者： Jairus B. Bowne Tim A. Erwin Juan Juttner Thorsten Schnurbusch Peter Langridge Antony Bacic ute roessner Australian Centre for Plant Functional Genomics School of Botany The University of Melbourne 3010 Victoria Australia Australian Centre for Plant Functional Genomics University of Adelaide Waite Campus Glen Osmond 5064 SA Australia Metabolomics Australia Bio21 Molecular Science and Biotechnology Institute 30 Flemington Rd The University of Melbourne 3010 Victoria Australia ARC Centre for Excellence for Plant Cell Walls School of Botany The University of Melbourne 3010 Victoria Australia Metabolomics Australia School of Botany The University of Melbourne 3010 Victoria Australia

Drought has serious effects on the physiology of cereal crops. At the cellular and specifically the metabolite level, many individual compounds are increased to provide osmoprotective functions, prevent the dissociation of enzymes, and to decrease the number of reactive oxygen species present in the cell. We have used a targeted GC-MS approach to identify compounds that differ in three different cultivars of bread wheat characterized by different levels of tolerance to drought under drought stress （Kukri, intolerant; Excalibur and RAC875, tolerant）. Levels of amino acids, most notably proline, tryptophan, and the branched chain amino acids leucine, isoleucine, and valine were increased under drought stress in all cultivars. In the two tolerant cultivars, a small decrease in a large number of organic acids was also evident. Excalibur, a cultivar genotypically related to Kukri, showed a pattern of response that was more similar to Kukri under well-watered conditions. Under drought stress, Excalibur and RAC875 had a similar response; however, Excalibur did not have the same magnitude of response as RAC875. Here, the results are discussed in the context of previous work in physiological and proteomic analyses of these cultivars under drought stress.

关键词： abiotic/environmental stress metabolomics drought wheat.

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Transition from a maternal to external nitrogen source in maize seedlings

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Journal of Integrative Plant Biology 2017年第4期59卷 261-274页

作者： Kasra Sabermanesh Luke R. Holtham Jessey George ute roessner Berin A. Boughton Sigrid Heuer Mark Tester Darren C. Plett Trevor P. Garnett Australian Centre for Plant Functional Genomics Waite Research Institute University of Adelaide Adelaide SA 5064 Australia School of Agriculture Food and Wine Waite Research Institute University of Adelaide Adelaide SA 5064 Australia Australian Centre for Plant Functional Genomics School of BioSciences University of Melbourne Parkville Vic. 3010 Australia Metabolomics Australia School of BioSciences University of Melbourne Vic. 3010 Australia King Abdullah University of Science and Technology Center for Desert Agriculture Thuwal 23955-6900 Kingdom of Saudi Arabia6. The Australian Plant Phenomics Facility The Plant Accelerator Waite Campus The University of Adelaide Adelaide SA 5064 Australia The Australian Plant Phenomics Facility The Plant Accelerator Waite Campus The University of Adelaide Adelaide SA 5064 Australia

Maximizing NO3 uptake during seedling development is important as it has a major influence on plant growth and yield. However, little is known about the processes leading to, and involved in, the initiation of root NO3 uptake capacity in developing seedlings. This study examines the physiological processes involved in root NO3 uptake and metabolism, to gain an understanding of how the NO3 uptake system responds to meet demand as maize seedlings transition from seed N use to external N capture. The concentrations of seedderived free amino acids within root and shoot tissues are initially high, but decrease rapidly until stabilizing eight days after imbibition （DAI）. Similarly, shoot N% decreases, but does not stabilize until 12-13 DAI. Following the decrease in free amino acid concentrations, root NO3- uptake capacity increases until shoot N% stabilizes. The increase in root NO3 uptake capacity corresponds with a rapid rise in transcript levels of putative NO3 transporters, ZmNRT2.1 and ZmNRT2.2. The processes underlying the increase in root NO3- uptake capacity to meet N demand provide an insight into the processes controlling N uptake.

关键词： NRT Transition from a maternal to external nitrogen source in maize seedlings NO

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Integrative Multi-omics Analyses of Barley Rootzones under Salinity Stress Reveal Two Distinctive Salt Tolerance Mechanisms

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Plant Communications 2020年第3期1卷 107-123页

作者： William Wing Ho Ho Camilla B.Hill Monika S.Doblin Megan C.Shelden Allison van de Meene Thusitha Rupasinghe Antony Bacic ute roessner School of BioSciences The University of MelbourneParkvilleVIC 3010Australia School of Veterinary and Life Sciences Murdoch UniversityMurdochWA 6150Australia La Trobe Institute for Agriculture&Food Department of AnimalPlant and Soil ScienceLa Trobe UniversityBundooraVIC 3086Australia ARC Centre of Excellence in Plant Energy Biology School of AgricultureFood and WineUniversity of AdelaideGlen OsmondSA 5064Australia Metabolomics Australia The University of MelbourneParkvilleVIC 3010Australia

The mechanisms underlying rootzone-localized responses to salinity during early stages of barley development remain *** this study,we performed the analyses of multi-root-omes(transcriptomes,metabolomes,and lipidomes)of a domesticated barley cultivar(Clipper)and a landrace(Sahara)that maintain and restrict seedling root growth under salt stress,*** generalized linear models were designed to determine differentially expressed genes(DEGs)and abundant metabolites(DAMs)specific to salt treatments,genotypes,or rootzones(meristematic Z1,elongation Z2,and maturation Z3).Based on pathway over-representation of the DEGs and DAMs,phenylpropanoid biosynthesis is the most statistically enriched biological pathway among all salinity responses *** with histological evidence,an intense salt-induced lignin impregnation was found only at stelic cell wall of Clipper Z2,compared with a unique elevation of suberin deposition across Sahara *** suggests two differential salt-induced modulations of apoplastic flow between the *** on the global correlation network of the DEGs and DAMs,callose deposition that potentially adjusted symplastic flow in roots was almost independent of salinity in rootzones of Clipper,and was markedly decreased in *** together,we propose two distinctive salt tolerance mechanisms in Clipper(growth-sustaining)and Sahara(salt-shielding),providing important clues for improving crop plasticity to cope with deteriorating global soil salinization.

关键词： barley root transcriptomics metabolomics lipidomics omics integration salinity stress

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Drought in wheat:combining genetics and metabolomics to identify novel drought tolerance-related traits

Drought in wheat:combining genetics and metabolomics to iden...

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第二届植物代谢国际会议

作者： Camilla Hill Jairus Bowne James Edwards Peter Langridge Antony Bacic ute roessner Australian Centre for Plant Functional Genomics The University of Adelaide Waite Campus Glen Osmond 5064 SA Australia

＜正＞The survival and reproduction of plants is entirely dependent on their ability to adapt to an unpredictable and dynamic environment. Traditional breeding programs for complex traits,such as abiotic stress

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Inactivation of Mitochondrial Complex Ⅰ Induces the Expression of a Twin Cysteine Protein that Targets and Affects Cytosolic, Chloroplastidic and Mitochondrial Function

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Molecular Plant 2016年第5期9卷 696-710页

作者： Yan Wang Wenhui Lyu Oliver Berkowitz Jordan D. Radomiljac Simon R. Law Monika W. Murcha Chris Carrie Pedro F. Teixeira Beata Kmiec Owen Duncan Olivier Van Aken Reena Narsai Elzbieta Glaser Shaobai Huang ute roessner A. Harvey Millar James Whelan Department of Animal Plant and Soil Science Australian Research Council Centre of Excellence in Plant Energy Biology School of Life Science La Trobe University Bundoora VIC 3086 Australia Ume(a)Plant Science Centre(UPSC) Faculty of Science and Technology Ume(a)University Ume(a) Sweden Australian Research Council Centre of Excellence in Plant Energy Biology University of Western Australia 35 Stirling Highway Crawley WA 6009 Australia Department of Biology I Botany Ludwig-Maximilians-Universitat Munchen Groβhaderner Strasse 2-4 82152 Planegg-Martinsried Germany Department of Biochemistry and Biophysics Stockholm University Atrhenius Laboratories for Natural Sciences 10691 Stockholm Sweden School of BioSciences The University of Melbourne Melbourne VIC 3010 Australia

At12Cys-1 （At5g64400） and At12Cys-2 （At5g09570） are two closely related isogenes that encode small, twin cysteine proteins, typically located in mitochondria. At12Cys-2 transcript is induced in a variety of mutants with disrupted mitochondrial proteins, but an increase in At12Cys protein is only detected in mutants with reduced mitochondrial complex I abundance. Induction of At12Cys protein in mutants that lack mitochondrial complex I is accompanied by At12Cys protein located in mitochondria, chloroplasts, and the cytosoh Biochemical analyses revealed that even single gene deletions, i.e., At12cys-1 orAtl2cys-2, have an effect on mitochondrial and chloroplast functions. However, only double mutants, i.e., At12cys-1：At12cys.2, affect the abundance of protein and mRNA transcripts encoding translation elongation factors as well as rRNA abundance. Blue native PAGE showed that At12Cys co-migrated with mitochondrial supercomplex I ＋ lU. Likewise, deletion of both At12cys-1 and At12cys-2 genes, but not single gene deletions, results in enhanced tolerance to drought and light stress and increased anti-oxidant capacity. The induction and multiple localization of At12Cys upon a reduction in complex I abundance provides a mechanism to specifically signal mitochondrial dysfunction to the cytosol and then beyond to other organelles in the cell.

关键词： mitochondria complex I retrograde signaling chloroplast cytosol

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