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The Liver X-Receptor (Lxr) Governs Lipid Homeostasis in Zebrafish during Development

Open Journal of Endocrine and Metabolic Diseases 2012年第4期2卷 74-81页

作者： Amena Archer Satish Srinivas Kitambi Stefan LHallgren Matteo Pedrelli KHakan olsén Agneta Mode Jan-Ake Gustafsson Department of Biosciences and Nutrition Karolinska InstituteSolnaSweden School of Life Sciences Sodertorns UniversitySodertornSweden Division of Clinical Chemistry Department of Laboratory MedicineKarolinska InstituteSolnaSweden Center for Nuclear Receptors and Cell Signaling Department of Biology and BiochemistryUniversity of HoustonHoustonUSA

The liver X-receptors (LXRs) act as cholesterol sensors and participate in the regulation of lipid and cholesterol metabolism. The objective of this study was to determine the role of LXR during development using the zebrafish model. By in situ hybridization we showed distinct expression of lxr in the brain and the retina in the developing and adult zebrafish. Lxr ligand activation affected the expression of genes involved in lipid metabolism in zebrafish adult brain and eye as well as in zebrafish embryos. Morpholino knock down of lxr resulted in an overall impaired lipid deposition as determined by oil red O staining particularly in the head and around the eyes, and to significantly elevated levels of both total and free cholesterol in the yolk of lxr morphant embryos. The expression of genes involved in lipid and cholesterol metabolism was also changed in the lxr morphants. Furthermore, alcian blue staining revealed malformation of the pharyngeal skeleton in the lxr morphant. Our data show that lxr is an important component of the regulatory network governing the lipid homeostasis during zebrafish development, which in turn may support a role of lxr for normal development of the central nervous sytem, including the retina.

关键词： Liver X-receptor Zebrafish Morpholino Lipid

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实验性短期电刺激引起的机体紊乱(英文)

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中国组织工程研究 2002年第8期6卷 1212-1213页

作者： Ireneusz M. Kowalski1, Jozef Szarek2, Daniel Zarzycki3(1.Regional Children Hospital and Rehabilitation Centre, 11 015 Ameryka, Poland 2.Department of Forensic and Administration Veterinary Medicine, University of Warmia and Mazury in olsztyn, 10 717 ols Regional Children Hospital Rehabilitation Centre 11-015 Ameryka Poland Department of Forensic and Administration Veterinary Medicine University of Warmia and Mazury in Olsztyn 10-717 Olsztyn Poland Department of Pediatric Orthopaedics and Rehabilitation Jagiellonian University College of Medicine 34-501 Zakopane Poland

Objective To determine the effect of LESS st on the organism of developing rabbits,and provide reference for the treatment of idiopathic *** Investigations were carried out on 10 rabbits, males, 3,5 months, New Zealand race, divided into two equal groups (n = 5): I in which short timed lateral electric surface stimulation (LESS st) was applied for 2 h daily during 3 months, II control group, where animals were not stimulated. LESS was performed with SCOL 2, a Polish stimulator, which was placed to the right of the rabbit spine, according to the method modified by Kowalski. After 3 months the animals were sacrificed, and macroscopic examination was performed. Statistical analysis based on Student's t test was carried out into account body mass and results of the macroscopic *** Post mortem examination of the internal organs showed no pathologic lesions in the animals both group. Only mean mass of suprarenes in electrostimulated rabbits was 42.7 % bigger than in control *** obtained results suggest that LESS st did not cause statistically significant disturbances of rabbit growth during a 3 month experiment. Small variations were observed only in the end phase of the *** (1) Short timed electrostimulation (2h/day) did not cause statistically significant disturbances of rabbit growth during a 3 month experiment.(2) Short timed LESS resulted in some behavioural disturbances in rabbits.

关键词： behaviour effect whole organism electrostimulation rabbit scoliosis

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