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Colorectal cancer cell growth inhibition by linoleic acid is related to fatty acid composition changes

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2010年第12期11卷 923-930页

作者： Xiao-feng LU Guo-qing HE Hai-ning YU Qi MA Sheng-rong SHEn undurti n. das Department of Food Science and nutrition College of Biosystems Engineering and Food Science Zhejiang University Hangzhou 310029 China College of Pharmaceutical Sciences Zhejiang University of Technology Hangzhou 310032 China UnD Life Sciences Shaker Heights OH 44120 USA College of Medicine Bharati Vidyapeeth University Pune 411030 India

Polyunsaturated fatty acids （PUFAs） possess anti-cancer action both in vitro and in vivo. In the present study, we detected cell viability with methyl thiazolyl tetrazolium （MTT） assay and cell membrane permeability with propidium iodide （PI） fluorescence dyeing, and calculated cell membrane fluidity change as fluorescence anisotropy. Fatty acid content in cells was measured by gas chromatography/mass spectroscopy （GC/MS）, and the relationship between fatty acid composition and cell viability was studied. We observed that n-6 PUFA linoleic acid （LA） inhibited tumor cell growth at high concentrations （〉300 μmol/L）, while low concentrations （100-200 μmol/L） seemed to promote cell proliferation. Analyses of cell membrane permeability, cell membrane fluidity, and cell fatty acid composition suggested that the anti-cancer action of LA could be related to changes in the ratio of n-6 to n-3 PUFAs. We observed that pre-incubation of cancer cells with 100 μmol/L LA for 24 h enhanced cell sensitivity to the cytotoxic action of LA, whereas undifferentiated cell line LoVo seemed to have a distinct path in LA-induced death. These results showed that one of the mechanisms by which supplementation of LA induces cancer cell death could be altering the ratio of n-6/n-3 PUFAs, and this may be related to celt differentiation status.

关键词： Anti-cancer Fatty acids composition Linoleic acid In vitro

Relationship between gut and sepsis:Role of ghrelin

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World Journal of Diabetes 2011年第1期2卷 1-7页

作者： undurti n das Jawaharlal nehru Technological University UnD Life Sciences Shaker Heights

Ghrelin is a growth hormone secretagogue producedby the gut,and is expressed in the hypothalamusand other tissues as *** not only plays animportant role in the regulation of appetite,energy balance and glucose homeostasis,but also shows antibacterial activity,suppresses pro-inflammatory cytokine production and restores gut barrier *** animals,ghrelin has shown significantbeneficial actions in preventing mortality from *** the critically ill,corticosteroid insufficiency as a resultof dysfunction of the hypothalamic-pituitary-adrenalaxis is known to *** is therefore possible that bothgut and hypothalamus play an important role in thepathogenesis of sepsis by virtue of their ability to produce ghrelin,which,in turn,could be a protective phenomenon to suppress *** remains to beseen whether ghrelin and its analogues are of benefit intreating patients with sepsis.

关键词： Ghrelin Sepsis Cytokines Inflammation Critically ill Insulin

Vagal nerve stimulation in prevention and management of coronary heart disease

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World Journal of Cardiology 2011年第4期3卷 105-110页

作者： undurti n das UnD Life Sciences School of Biotechnology Jawaharlal Nehru Technological University Department of Medicine Krishna Institute of Medical Sciences

Coronary heart disease (CHD) that is due to atherosclerosis is associated with low-grade systemic inflammation. Congestive cardiac failure and arrhythmias that are responsible for mortality in CHD can be suppressed by appropriate vagal stimulation that is anti-inflammatory in nature. Acetylcholine, the principal vagal neurotransmitter, is a potent anti-inflammatory molecule. Polyunsaturated fatty acids (PUFAs) augment acetylcholine release, while acetylcholine can enhance the formation of prostacyclin, lipoxins, resolvins, protectins and maresins from PUFAs, which are anti-inflammatory and anti-arrhythmic molecules. Furthermore, plasma and tissue levels of PUFAs are low in those with CHD and atherosclerosis. Hence, vagal nerve stimulation is beneficial in the prevention of CHD and cardiac arrhythmias. Thus, measurement of catecholamines, acetylcholine, various PUFAs, and their products lipoxins, resolvins, protectins and maresins in the plasma and peripheral leukocytes, and vagal tone by heart rate variation could be useful in the prediction, prevention and management of CHD and cardiac arrhythmias.

关键词： Vagal nerve stimulation Acetylcholine Coronary heart disease Cardiac arrhythmias Heart failure

Hypothesis:Intensive insulin therapy-induced mortality is due to excessive serotonin autoinhibition and autonomic dysregulation

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World Journal of Diabetes 2010年第4期1卷 101-108页

作者： undurti n das Jawaharlal nehru Technological University UnD Life Sciences Shaker Heights

Action to Control Cardiovascular Risk in Diabetes(ACCORD),The Action in Diabetes and Vascular Disease:Preterax and Diamicron Modified Release Controlled Evaluation and the Veterans Affairs Diabetes Trial were designed to study whether older patients with type 2 diabetes mellitus could reduce the risk of heart attacks and stroke and thereby prolong their lives by maintaining their blood glucose levels at near-healthy levels but failed to demonstrate the hoped-for benef *** the trials failed,though,and why ACCORD saw significantly more deaths due to increased rates of cardiovascular events in the intensive therapy arm of the study are not *** data have now been confirmed by the results of the recently concluded nICE-SUGAR Study which again revealed that intensive gluc ose control increased mortality among adults in intensive care units.I propose that the negative results noted in these trials are due to altered brain serot o nin concentrations and autonomic dysregulation in addition to the low-grade systemic inflammation,decreased endothelial nitric oxide and enhanced free radical generation,diminished anti-oxidant defenses and altered metabol ism of essential fatty acids present in patients with type 2 diabetes.

关键词： Type 2 diabetes mellitus Cardiovascular events Coronary heart disease Stroke Dyslipidemia Essential fatty acids nitric oxide Free radicals Antioxidants Insulin

A defect in the activities of △~6 and △~5 desaturases and pro-resolution bioactive lipids in the pathobiology of non-alcoholic fatty liver disease

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World Journal of Diabetes 2011年第11期2卷 176-188页

作者： undurti n das UnD Life Sciences School of Biotechnology Jawaharlal Nehru Technological University Bio-Science Research Centre Gayatri Vidya Parishad College of Engineering Visakhapatnam-530 048

non-alcoholic fatty liver disease (nAFLD) is a low-grade systemic inflammatory condition, since liver and adipose tissue tumor necrosis factor-α (TnF-α) and TnF receptor 1 transcripts and serum TnF-α levels are increased and IL-6-/-mice are less prone to nAFLD. Fatty liver damage caused by high-fat diets is associated with the generation of pro-inflammatory prostaglandin E2 (PGE2). A decrease in the levels of arachidonic acid (AA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) and the usefulness of EPA and DHA both in the prevention and management of nAFLD has been reported. AA, EPA and DHA and their anti-inflammatory products lipoxins (LXs), resolvins and protectins suppress IL-6 and TnF-α and PGE2 production. These results suggest that the activities of △6 and △5 desaturases are reduced in nAFLD and hence, the dietary essential fatty acids, linoleic acid (LA) and α-linolenic acid (ALA) are not metabolized to their long-chain products AA, EPA and DHA, the precursors of anti-inflammatory molecules, LXs, resolvins and protectins that could prevent nAFLD. This suggests that an imbalance between proand anti-inflammatory bioactive lipids contribute to nAFLD. Hence, it is proposed that plasma and tissue levels of AA, EPA, DHA and LXs, resolvins and protectins could be used as predictors and prognostic biomarkers of nAFLD. It is suggested that the synthesis and use of more stable analogues of LXs, resolvins and protectins need to be explored in the prevention and management of nAFLD.

关键词： Prostaglandins Lipids Arachidonic acid Eicosapentaenoic acid non-alcoholic fatty liver disease Docosahexaenoic acid Lipoxins Resolvins Protectins Cytokines Free radicals Hyperlipidemia

Hydrolyzates of Silkworm Pupae(Bombyx mori)Protein is a new source of Angiotensin I-Converting Enzyme Inhibitory Peptides(ACEIP)

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Hydrolyzates of Silkworm Pupae(Bombyx mori)Protein is a new ...

Epigallocatechin-3-gallate affects the growth of LnCaP cells via membrane fluidity and distribution of cellular zinc

中国食品科学技术学会第五届年会暨第四届东西方食品业高层论坛

作者： undurti n das Department of Food Science and nutrition Zhejiang University Hangzhou310029P.R.China UND Life Sciences13800 Fairhill Road321 Shaker HeightsOH 44120USA

Silkworm pupae protein is a good source of high quality *** hydrolyzates of silkworm pupae protein catalyzed by neutrase,pepsin,acidic protease(Asperqiius usamii nO. 537),flavourzyme,alcalase,and trypsin with inhibitory activity on angiotensin I-converting enzyme(ACE)were identified by *** hydrolyzates catalyzed by acidic protease exerted the highest inhibitory activity on *** hydrolyzing conditions were optimized by one-factor, fractional factorial(FFD),and center composite(CCD)design methods,and response surface methodology(RSM).Statistical analyses showed that regression of the second-order model equation is suitable to describe ACE inhibitory *** predicted inhibitory activity of hydrolyzates on ACE was 73.48% at a concentration of 2.0 mg/*** RSM technique decreased IC50 of hydrolyzates inhibiting ACE to 1.38mg/mL from 2.5mg/*** molecular weight of the components of the hydrolyzates with inhibitory activity on ACE varied from 500 to 1000 Da by ultra-filter *** studies suggest that hydrolyzates of silkworm protein contain ACE inhibitory activity that could form a potential source of ACE inhibitor drugs.

关键词： Angiotensin converting enzyme silkworm pupae protein Bombyx mori hypertension,hydrolyze

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2009年第6期10卷 411-421页

作者： Jun-guo YAnG Hai-ning YU Shi-li SUn Lan-cui ZHAnG Guo-qing HE undurti n. das Hui RUAn Sheng-rong SHEn Department of Food Scienee and nutrition Zhejiang University Hangzhou 310029 China College of Pharmaceutical Sciences Zhejiang University of Technology Hangzhou 310032 China Tea Research Institute Guangdong Academy of Agricultural Sciences Guangzhou 510640 China UnD Life Sciences 13800 Fairhill Road #321 Shaker Heights OH 44120 USA

Objective:To evaluate effects of epigallocatechin-3-gallate (EGCG) on the viability, membrane properties, and zinc distribution, with and without the presence of Zn2+, in human prostate carcinoma LnCaP cells. Methods: We examined changes in cellular morphology and membrane fluidity of LnCaP cells, distribution of cellular zinc, and the incorporated portion of EGCG after treatments with EGCG, Zn2+, and EGCG+Zn2+. Results: We observed an alteration in cellular morphology and a decrease in membrane fluidity of LnCaP cells after treatment with EGCG or Zn2+. The proportion of EGCG incorporated into liposomes treated with the mixture of EGCG and Zn2+ at the ratio of 1:1 was 90.57%, which was significantly higher than that treated with EGCG alone (30.33%). Electron spin resonance (ESR) studies and determination of fatty acids showed that the effects of EGCG on the membrane fluidity of LnCaP were decreased by Zn2+. EGCG accelerated the accumulation of zinc in the mitochondria and cytosol as observed by atomic absorption spectrometer. Conclusion: These results show that EGCG interacted with cell membrane, decreased the membrane fluidity of LnCaP cells, and accelerated zinc accumulation in the mitochondria and cytosol, which could be the mechanism by which EGCG inhibits proliferation of LnCaP cells. In addition, high concentrations of Zn2+ could attenuate the actions elicited by EGCG.

关键词： Epigallocatechin-3-gallate (EGCG) Zinc LnCaP Membrane fluidity

茶籽中脂肪酸的组成及其生物活性脂肪酸对高糖胁迫下RF/6A细胞生长的影响

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茶叶科学 2009年第6期29卷 419-425页

作者：于海宁单伟光 undurti n das 沈生荣浙江工业大学药学院浙江大学食品科学与营养学系 UnD Life Sciences 13800 Fairhill Road#321Shaker HeightsOH44120USA

采用气相色谱(GC)对茶籽的脂肪酸组成进行分析,并研究了活性脂肪酸对高浓度葡萄糖胁迫下RF/6A细胞生长的影响及其与葡萄糖对RF/6A细胞脂肪酸代谢的调节。结果表明,油酸与亚油酸为茶籽中含量最高的脂肪酸;而茶籽中脂肪酸的组成与其品种... 详细信息

采用气相色谱(GC)对茶籽的脂肪酸组成进行分析,并研究了活性脂肪酸对高浓度葡萄糖胁迫下RF/6A细胞生长的影响及其与葡萄糖对RF/6A细胞脂肪酸代谢的调节。结果表明,油酸与亚油酸为茶籽中含量最高的脂肪酸;而茶籽中脂肪酸的组成与其品种和产地有密切关系。亚油酸与亚麻酸对高浓度葡萄糖诱导的RF/6A细胞过度增殖有抑制作用。亚油酸、亚麻酸及棕榈酸对RF/6A细胞的脂肪酸代谢均具有调节作用。

关键词：茶籽脂肪酸 RF/6A细胞高糖胁迫

Dielectric relaxation and magneto-electric characteristics of lead-free double perovskite: Sm2niMnO6

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Journal of Advanced Ceramics 2019年第2期8卷 174-185页

作者： Rutuparna das R.n.P.CHOUDHARY Department of Physics Siksha ‘O’ Anusandhan University

The polycrystalline sample of a double perovskite, Sm2niMnO6 was synthesized by a solid-state reaction route. From the X-ray structural study, it is found that the structure of the material is monoclinic with lattice parameters: a = 4.1750(63) ?, b = 7.6113(63) ?, c = 5.9896(63) ?, and β = 112.70°. These parameters are very close to and consistent with those of such type of materials. The dielectric, impedance, AC conductivity, and electrical modulus properties of the sample were studied in the temperature range of 25–300℃ and the frequency range of 1 kHz–1 MHz. Typical relaxor behavior observed in the dielectric studies was confirmed by Vogel–Fulcher fitting. From the nyquist plots, the temperature dependent contribution of grain and grain boundary effect was confirmed. The non-Debye type of relaxation was found using the complex impedance spectroscopy. The magnetic study revealed that the sample had paramagnetic behavior at room temperature. Magneto-electric(ME) coefficient was obtained by changing DC bias magnetic field. This type of lead-free relaxor ferroelectric compound may be useful for high-temperature applications.

关键词： solid-state reaction X-ray diffraction(XRD) dielectric relaxation magneto-electric(ME) coupling