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Small mammal community composition impacts bank vole (Clethrionomys glareolus) population dynamics and associated seroprevalence of Puumala orthohantavirus

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Integrative Zoology 2024年第1期19卷 52-65页

作者： Felicitas maria BUJNOCH Daniela REIL Stephan DREWES ulrike m.osenfeld Rainer G.ULRICH Jens JACOB Christian ImHOLT Julius Kühn-Institute Federal Research Centre for Cultivated PlantsInstitute for Epidemiology and Pathogen DiagnosticsRodent ResearchMünsterGermany University of münster Institute for Evolution and BiodiversityMünsterGermany Friedrich-Loeffler-Institut Federal Research Institute for Animal HealthInstitute of Novel and Emerging Infectious DiseasesGreifswald-Insel RiemsGermany

Rodents are important reservoirs for zoonotic pathogens that cause diseases in *** is hypoth-esized to be closely related to pathogen prevalence through multiple direct and indirect *** example,the presence of non-host species can reduce contact rates of the main reservoir host and thus reduce the risk of transmission(“dilution effect”).In addition,an overlap in ecological niches between two species could lead to increased interspecific competition,potentially limiting host densities and reducing density-dependent pathogen transmission *** this study,we investigated the relative impact of population-level regulation of direct and indirect drivers of the prevalence of Puumala orthohantavirus(PUUV)in bank voles(Clethrionomys glareolus)during years with high *** compiled data on small mammal community composition from four regions in Germany between 2010 and *** equation modeling revealed a strong seasonality in PUUV control mechanisms in bank *** abundance of shrews tended to have a negative relationship with host abundance,and host abundance positively influenced PUUV seroprevalence,while at the same time increasing the abundance of competing non-hosts like the wood mouse(Apodemus sylvaticus)and the yellow-neckedfield mouse(Apodemusflavicollis)were associated with reduced PUUV seroprevalence in the *** results indicate that for PUUV in bank voles,dilution is associated with increased interspecific *** pressures leading to the decline of Apodemus *** a specific habitat could lead to the amplification of mechanisms promoting PUUV transmission within the host populations.

关键词： bank vole biodiversity Puumala orthohantavirus small mammals

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Development of an active-site titrant for SARS-CoV-2 main protease as an indispensable tool for evaluating enzyme kinetics

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Acta Pharmaceutica Sinica B 2024年第5期14卷 2349-2357页

作者： Rabea Voget Julian Breidenbach Tobias Claff Alexandra Hingst Katharina Sylvester Christian Steinebach Lan Phuong Vu Renato H.Weiße ulrike Bartz Norbert Sträter Christa E.müller michael Gütschow Pharmaceutical Institute Pharmaceutical&Medicinal ChemistryUniversity of BonnBonn 53121Germany Institute of Bioanalytical Chemistry Center for Biotechnology and BiomedicineLeipzig UniversityLeipzig 04103Germany Department of Natural Sciences University of Applied Sciences Bonn-Rhein-SiegRheinbach 53359Germany

A titrant for the SARS-CoV-2 main protease(m^(pro))was developed that enables,for the first time,the exact determination of the concentration of the enzymatically active m^(pro) by active-site *** covalent binding mode of the tetrapeptidic titrant was elucidated by the determination of the crystal structure of the enzyme–titrant *** fluorogenic substrates of m^(pro),including a prototypical,internally quenched Dabcyl-EDANS peptide,were compared in terms of solubility under typical assay *** exploiting the new titrant,key kinetic parameters for the m^(pro)-catalyzed cleavage of these substrates were determined.

关键词： COVID-19 SARS-CoV-2 main protease Peptide nitriles Fluorogenic substrates Active-site titration X-ray crystallography Inner filter effect

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An orally-available monovalent SmAC mimetic compound as a broad-spectrum antiviral

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Protein & Cell 2024年第1期15卷 69-75页

作者： miao mei maria_Antonietta Impagnatiello Jun Jiao Ulrich Reiser ulrike Tontsch-Grunt Ju Zhang Paul Nicklin Bingke Yu Yu Wang Yuan He Xu Tan Tsinghua-Peking Center for Life Sciences Beijing Advanced Innovation Center for Structural BiologyBeijing Frontier Research Center for Biological StructureMOE Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical BiologySchool of Pharmaceutical SciencesTsinghua UniversityBeijing 100084China Chinese Institutes for medical Research Beijing 100069China Boehringer Ingelheim RCV GmbH&Co KG ViennaAustria College of Life Sciences and Oceanography Shenzhen UniversityShenzhen 518060China Research Beyond Borders Boehringer IngelheimBiberach an der RissGermany Research Beyond Borders Boehringer IngelheimShanghai 200120China

DearEditor,The coronavirus disease 2019(COVID-19)pandemic caused great global morbidity and mortality concurrent with astronomical socioeconomic damages and *** now,the culpable pathogen,SARS-CoV-2,has infected over 6 hundred million people resulting in more than 6 million deaths worldwide(WHO SARSCoV-2 cases).There is an urgent need for rapid development of effective and affordable therapeutics for COVID-19 patients.

关键词： mortality spectrum compound

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Do human activities influence survival and orientation abilities of larval fishes in the ocean?

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Integrative Zoology 2015年第1期10卷 65-82页

作者： ulrike E.SIEBECK Jack O’CONNOR Christoph BRAUN Jeffrey m.LEIS School of Biomedical Sciences The University of QueenslandBrisbaneAustralia Ichthyology The Australian MuseumSydneyAustralia Department of Environmental Studies University of Technology SydneyUltimoAustralia Institute for marine and Antarctic Studies University of TasmaniaHobartAustralia

The larval stages of most marine fishes spend days to weeks in the pelagic environment,where they must find food and avoid predators in order to *** fish only spend part of their life history in the pelagic environment before returning to their adult habitat,for example,a coral *** sensory systems of larval fish develop rapidly during the first few days of their lives,and here we concentrate on the various sensory cues the fish have available to them for survival in the pelagic *** focus on the larvae of reef fishes because most is known about *** also review the major threats caused by human activities that have been identified to have worldwide impact and evaluate how these threats may impact larval-fish survival and orientation *** human activities negatively affect larval-fish sensory systems or the cues the fish need to ***,this could lead to decreased numbers of larvae surviving to settlement,and,therefore,to decreased abundance of adult *** we focus on species wherein the larvae and adults occupy different habitats(pelagic and demersal,respectively),it is important to acknowledge that the potential anthropogenic effects we identify may also apply to larvae of species like tuna and herring,where both larvae and adults are pelagic.

关键词： climate change larval fish pollution sensory systems teleost fish

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A Biobank for Long-term and Sustainable Research in the Field of Congenital Heart Disease in Germany

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Genomics, Proteomics & Bioinformatics 2016年第4期14卷 181-190页

作者： Thomas Pickardt Eva Niggemeyer ulrike m.m.Bauer Hashim Abdul-Khaliq Competence Network for Congenital Heart Defects Investigators National Register for Congenital Heart Defects 13353 BerlinGermany Saarland University medical Center Department of Paediatrie Cardiology66421 HomburgGermany Competence Network for Congenital Heart Defects 13353 BerlinGermany

Congenital heart disease （CHD） is the most fi＇equent birth defect （0.8% 1% of all live births）. Due to the advance in prenatal and postnatal early diagnosis and treatment, more than 90% of these patients survive into adulthood today. However, several mid- and long-term morbidi- ties are dominating the follow-up of these patients. Due to the rarity and heterogeneity of the phenotypes of CHD, multicenter registry-based studies are required. The CHD-Biobank was established in 2009 with the aim to collect DNA from patients and their parents （trios） or fi＇om affected families, as well as cardiovascular tissues from patients undergoing corrective heart surgery for cardiovascular malformations. Clinical/phenotype data are matched to the International Paediatric and Congenital Cardiac Code （IPCCC） and the International Statistical Classification of Diseases and Related Health Problems 10th Revision （ICD-10）. The DNA collection currently comprises samples from approximately 4200 participants with a wide range of CHD phenotypes. The collection covers about 430 trios and 120 families with more than one affected member. The cardiac tissue collection comprises 1143 tissue samples [Yore 556 patients alter open heart surgery. The CHD-Biobank provides a comprehensive basis for research in the field of CHD with high standards of data privacy, IT management, and sample logistics.

关键词： Congenital heart defects multi-center research Biorepository Genetic research DNA

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The effect of temperature on ionic liquid modified Fe-N-C catalysts for alkaline oxygen reduction reaction

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Journal of Energy Chemistry 2022年第5期31卷 324-329页

作者： Thomas Wolker Kai Brunnengräber Ioanna martinaiou Nick Lorenz Gui-Rong Zhang ulrike I.Kramm Bastian J.m.Etzold Department of Chemistry Ernst-Berl-Institut für Technische und Makromolekulare ChemieTechnical University of Darmstadt64287 DarmstadtGermany Department of Chemistry Catalysts and Electrocatalysts GroupTechnical University of Darmstadt64287 DarmstadtGermany Graduate School of Excellence Energy Science and Engineering Technical University of Darmstadt64287 DarmstadtGermany Department of materials and Earth Sciences Technical University of Darmstadt64287 DarmstadtGermany School of Chemical Engineering and Technology Tiangong UniversityTianjin 300387China

modifying solid catalysts with an ionic liquid layer is an effective approach for boosting the performance of both Pt-based and non-precious metal catalysts toward the oxygen reduction reaction. While most studies operated at room temperature it remains unclear whether the IL-associated boosting effect can be maintained at elevated temperature, which is of high relevance for practical applications in low temperature fuel cells. Herein, Fe-N-C catalysts were modified by introducing small amounts of hydrophobic ionic liquid, resulting in boosted electrocatalytic activity towards the alkaline oxygen reduction reaction at room temperature. It is demonstrated that the boosting effect can be maintained and even strengthened when increasing the electrolyte temperature up to 70℃. These findings show for the first time that the incorporation of ionic liquid is a suited method to obtain advanced noble metal-free electrocatalysts that can be applied at operating temperature condition.

关键词： Oxygen reduction reaction Non-precious metal catalyst Ionic liquid Fe-N-C catalyst Temperature effect

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Innate immune recognition and modulation in hepatitis D virus infection

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World Journal of Gastroenterology 2020年第21期26卷 2781-2791页

作者： Stephanie Jung Sebastian maximilian Altstetter ulrike Protzer Institute of Virology Technical University of Munich/Helmholtz Zentrum MünchenMunich D-81675Germany German Center for Infection Research(DZIF) Munich Partner SiteMunich D-81675Germany

Hepatitis D virus(HDV)is a global health threat with more than 15 million humans *** treatment options are largely unsatisfactory leaving chronically infected humans at high risk to develop liver cirrhosis and hepatocellular *** is the only human satellite virus *** encodes only two proteins,and requires Hepatitis B virus(HBV)envelope protein expression for productive virion release and spread of the *** HDV could evolve and why HBV was selected as a helper virus remains *** the discovery of Na+-taurocholate co-transporting polypeptide as the essential uptake receptor for HBV and HDV,we are beginning to understand the interactions of HDV and the immune *** HBV is mostly regarded a stealth virus,that escapes innate immune recognition,HBV-HDV coinfection is characterized by a strong innate immune *** RNA sensor melanoma differentiation antigen 5 has been reported to recognize HDV RNA replication and activate innate *** immunity,however,seems not to impair HDV replication while it inhibits *** this review,we describe what is known up-to-date about the interplay between HBV as a helper and HDV’s immune evasion strategy and identify where additional research is required.

关键词： Hepatitis D virus Hepatitis B virus Innate immunity Pathogen-associated molecular pattern molecules Immune evasion Immunosuppression

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Complementarity of lacustrine pollen and sedimentary DNA in representing vegetation on the central-eastern Tibetan Plateau

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Frontiers of Earth Science 2023年第4期17卷 1037-1048页

作者： Fang TIAN meijiao CHEN Weihan JIA ulrike HERZSCHUH Xianyong CAO College of Resource Environment and Tourism Capital Normal UniversityBeijing 100048China Alfred Wegener Institute Helmholtz Centre for Polar and marine Research Polar Terrestrial Environmental Systems Research GroupPotsdam 14473Germany University of Potsdam Institute of Environmental Science and GeographyPotsdam 14476Germany University of Potsdam Institute of Biochemistry and BiologyPotsdam 14476Germany Group of Alpine Paleoecology and Human Adaptation(ALPHA) State Key Laboratory of Tibetan Plateau Earth SystemEnvironment and Resources(TPESER)Institute of Tibetan Plateau ResearchChinese Academy of SciencesBeijing 100101China

Plant environmental DNA extracted from lacustrine sediments(sedimentary DNA,sedDNA)has been increasingly used to investigate past vegetation changes and human impacts at a high taxonomic ***,the representation of vegetation communities surrounding the lake is still *** this study,we compared plant sedDNA metabarcoding and pollen assemblages from 27 lake surface-sediment samples collected from alpine meadow on the central-eastern Tibetan Plateau to investigate the representation of sedDNA *** general,the identified components of sedDNA are consistent with the counted pollen taxa and local plant *** to pollen identification,sedDNA data have higher taxonomic resolution,thus providing a potential approach for reconstructing past plant *** sedDNA signal is strongly influenced by local plants while rarely affected by exogenous *** of the overrepresentation of local plants and PCR bias,the abundance of sedDNA sequence types is very variable among sites,and should be treated with caution when investigating past vegetation cover and climate based on sedDNA *** finding suggests that sedDNA analysis can be a complementary approach for investigating the presence/absence of past plants and history of human land-use with higher taxonomic resolution.

关键词： Tibetan Plateau plant DNA metabarcoding pollen lake sediment plant diversity vegetation history

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Sifu-a cybersecurity awareness platform with challenge assessment and intelligent coach

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Cybersecurity 2018年第1期1卷 945-967页

作者： Tiago Espinha Gasiba ulrike Lechner maria Pinto-Albuquerque Siemens AG Corporate Technology Otto-Hahn-Rin 681379 MunichBavariaGermany Universitat der Bundeswehr münchen MunichGermany Instituto Universitario de Lisboa(ISCTE-IUL) ISTAR-IULLisbonPortugal

Software vulnerabilities,when actively exploited by malicious parties,can lead to catastrophic *** handling of software vulnerabilities is essential in the industrial context,particularly when the software is deployed in critical ***,several industrial standards mandate secure coding guidelines and industrial software developers’training,as software quality is a significant contributor to secure *** Challenges(CSC)form a method that combines serious game techniques with cybersecurity and secure coding guidelines to raise secure coding awareness of software developers in the *** cybersecurity awareness events have been used with success in industrial ***,until now,these coached events took place *** the present work,we briefly introduce cybersecurity challenges and propose a novel platform that allows these events to take place *** introduced cybersecurity awareness platform,which the authors call Sifu,performs automatic assessment of challenges in compliance to secure coding guidelines,and uses an artificial intelligence method to provide players with solution-guiding ***,due to its characteristics,the Sifu platform allows for remote(online)learning,in times of social *** CyberSecurity Challenges events based on the Sifu platform were evaluated during four online real-life CSC *** report on three surveys showing that the Sifu platform’s CSC events are adequate to raise industry software developers awareness on secure coding.

关键词： Cybersecurity Awareness Training Artificial intelligence Serious games Secure coding Static application security testing Capture-the-flag Software development in industry

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Expression liver-directed genes by employing synthetic transcriptional control units

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World Journal of Gastroenterology 2005年第34期11卷 5295-5302页

作者： marie-Luise Lemken Wolfgang A.Wybranietz ulrike Schmidt Florian Graepler Sorin Armeanu michael Bitzer Ulrich m.Lauer Department of Internal medicine I University Clinic Tübingen D-72076 Tübingen Germany

AIm： To generate and characcerize the synthetic transoiptional control units for transcriptional targeting of the liver, thereby compensating for the lack of specificity of currently available gene therapeutic vector systems. mETHODS： Synthetic transcriptional control unit constructs were generated and analyzed for transcriptional activities in different cell types by FACS quantification, semi-quantitative RT-PCR, and Western blotting. RESULTS： A new bifunctionally-enhanced green fluorescent protein （EGFP）/neor fusion gene cassette was generated, and could flexibly be used both for transcript quantification and for selection of stable cell clones. Then, numerous synthetic transcriptional control units consisting of a minimal promoter linked to “naturally” derived composite enhancer elements from liver-specific expressed genes or binding sites of liver-specific transcription factors were inserted upstream of this reporter cassette. Following liposome-mediated transfection, EGFP reporter protein quantification by FACS analysis identified constructs encoding multimerized composite elements of the apolipoprotein B100 （APOB） promoter or the ornithin transcarbamoylase （OTC） enhancer to exhibit maximum transcriptional activities in liver originating cell lines, but only background levels in non-liver originating cell lines. In contrast, constructs encoding only singular binding sites of liver-specific transcription factors, namely hepatocyte nuclear factor （HNF）, HNF3, HNF4, HNF5, or CAAT/enhancer binding protein （C/EBP） only achieved background levels of EGFP expression. Finally, both semi-quantitative RT-PCR and Western blotting analysis of Hep3B cells demonstrated maximum transcriptional activities for a multimeric 4xAPOB cassette construct, which fully complied with the dataobtained by initial FACS analysis CONCLUSION： Synthetic transcriptional control unit constructs not only exhibit a superb degree of structural compactness, but also provide new means for liver

关键词： Liver gene therapy Hepatoma gene therapy Liver-directed gene expression Synthetic liver-specific promoters

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