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Scalable integration of nano-,and microfluidics with hybrid two-photon lithography

Microsystems & Nanoengineering 2019年第1期5卷 264-272页

作者： Oliver Vanderpoorten Quentin Peter Pavan K.Challa ulrich f.keyser Jeremy Baumberg Clemens f.Kaminski Tuomas P.J.Knowles Department of Chemical Engineering and Biotechnology University of CambridgePhilippa Fawcett DriveCambridge CB30ASUK Department of Chemistry University of CambridgeLensfield RoadCambridge CB21EWUK Cavendish Laboratory Department of PhysicsUniversity of CambridgeJ.J.Thomson AvenueCambridge CB30HEUK

Nanofluidic devices have great potential for applications in areas ranging from renewable energy to human health.A crucial requirement for the successful operation of nanofluidic devices is the ability to interface them in a scalable manner with the outside ***,we demonstrate a hybrid two photon nanolithography approach interfaced with conventional mask whole-wafer UV-photolithography to generate master wafers for the fabrication of integrated micro and nanofluidic *** this approach we demonstrate the fabrication of molds from SU-8 photoresist with nanofluidic features down to 230 nm lateral width and channel heights from micron to sub-100 *** electron microscopy and atomic force microscopy were used to characterize the printing capabilities of the system and show the integration of nanofluidic channels into an existing microfluidic chip *** functionality of the devices was demonstrated through super-resolution microscopy,allowing the observation of features below the diffraction limit of light produced using our *** molecule localization of diffusing dye molecules verified the successful imprint of nanochannels and the spatial confinement of molecules to 200 nm across the nanochannel molded from the master *** approach integrates readily with current microfluidic fabrication methods and allows the combination of microfluidic devices with locally two-photon-written nano-sized functionalities,enabling rapid nanofluidic device fabrication and enhancement of existing microfluidic device architectures with nanofluidic features.

关键词： lithography integration fluid

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A Bayesian approach for integrating multilevel priors and data for aerospace system reliability assessment

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Chinese Journal of Aeronautics 2018年第1期31卷 41-53页

作者： Jian GUO Zhaojun LI Thomas keyser Department of Industrial Engineering and Engineering Management Western New England University

This paper investigates Bayesian methods for aerospace system reliability analysis using various sources of test data and expert knowledge at both subsystem and system levels. four sce- narios based on available information for the priors and test data of a system and/or subsystems are studied using specific Bayesian inference techniques. This paper proposes the Bayesian melding method for integrating subsystem-level priors with system-level priors for both system- and subsystem-level reliability analysis. System and subsystem reliability outcomes are compared under different scenarios. Computational challenges for posterior inferences using the sophisticated Bayesian melding method are addressed using Markov Chain Monte Carlo （MCMC） and adaptive Sam- piing Importance Re-sampling （SIR） methods. A case study with simulation results illustrates the applications of the proposed methods and provides insights for aerospace system reliability analysis using available multilevel information.

关键词： Bayesian inference Bayesian melding Multilevel information Markov Chain Monte Carlo (MCMC) Sampling Importance Resampling (SIR) System reliability

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Model Identification of a Micro Air Vehicle

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Journal of Bionic Engineering 2007年第4期4卷 227-236页

作者： Jorge Nio flavius Mitrache Peter Cosyn Robin De keyser Department of Electrical Energy Systems and Automation Ghent University Technologiepark 913 9052 Ghent Belgium Department of flow Heat and Combustion Mechanics Ghent University Sint-Pietersnieuwstraat 41 9000 Ghent Belgium

This paper is focused on the model identification of a Micro Air Vehicle （MAV） in straight steady flight condition. The identification is based on input-output data collected from flight tests using both frequency and time dorrtain techniques. The vehicle is an in-house 40 cm wingspan airplane. Because of the complex coupled, multivariable and nonlinear dynamics of the aircraft, linear SISO structures for both the lateral and longitudinal models around a reference state were derived. The aim of the identification is to provide models that can be used in future development of control techniques for the MAV.

关键词： Micro Air Vehicles system identification flight tests

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Quality of life in glaucoma patients after selective laser trabeculoplasty

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International Journal of Ophthalmology(English edition) 2017年第5期10卷 742-748页

作者： Myrjam De keyser Maya De Belder Veva De Groot Department of Medicine University of Antwerp Universiteitsplein 1 Antwerp B-2610 Belgium Department of Experimental Psychology Ghent UniversityHenri Dunantlaan 2 Ghent B-9000 Belgium Department of Ophthalmology University Hospital AntwerpWilrijkstraat 10 Edegem B-2650 Belgium

AIM：To compare quality of life and treatment satisfaction between patients who had selective laser trabeculoplasty（SLT） and those on ***：A prospective clinical trial on 143 glaucoma patients that received SLT and a control group that continued using anti glaucoma medication was conducted. Tear break-up time（BUT）, punctuate keratitis, need for help, use of artificial tears and the treatment satisfaction survey of intraocular pressure（IOP） were measured at baseline, 6 and 12 mo. RESULTS：SLT was able to reduce the mean number of medications needed from 1.56±0.81 to 0.42±0.66 at six months and to 0.33±0.69 at one year. Punctuate keratitis was observed significantly less often（12.24%） after SLT than before（35.94%; P=0.03）. Use of artificial tears and BUT did not change significantly after SLT（P〉0.05）. At baseline, patients in the SLT group were significantly less convinced of medication effectiveness（P=0.006） and complained more about side effects（P=0.003）. After SLT, these patients had significantly more confidence in their therapy（P〈0.001）, showed less side effects（P=0.006）, complained less about changes in appearance of the eyes（P=0.003） and were less inconvenienced by the use of eye drops（P〈0.001）.CONCLUSION：SLT is able to improve treatment-related quality of life in glaucoma patients.

关键词： glaucoma laser treatment medical treatment quality of life

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Selective laser trabeculoplasty in pseudophakic and phakic eyes:a prospective study

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International Journal of Ophthalmology(English edition) 2017年第4期10卷 593-598页

作者： Myrjam De keyser Maya De Belder Veva De Groot Department of Medicine University of Antwerp Universiteitsplein Antwerp B-2610 Belgium Department of Experimental Psychology Faculty of Psychology and Education Ghent University Henri DunantlaanGhent B-9000 Belgium Department of Ophthalmology Antwerp University HospitalWilrijkstraat Edegem B-2650 Belgium

AIM：To compare the efficacy of selective laser trabeculoplasty（SLT） in replacing medical therapy in pseudophakic and phakic ***：Subgroup of a prospective randomized clinical trial including patients with primary open angle glaucoma or ocular hypertension controlled with *** 38 pseudophakic eyes were matched with 38 phakic *** was offered as a way to decrease medication while maintaining the same low eye *** was performed over 360°,at 3ns,spotsize 400 μm,100 *** [intraocular pressure（IOP）,number of medications needed] were measured at 1h,1wk,1,3,6 and 12 *** independent-samples t-test was performed to compare baseline characteristics of the phakic and the pseudophakic group and differences in evolution of mean IOP and number of used ***-squared analysis was performed to investigate proportions of fast,slow and ***：The mean IOP measurement was 13.00± 2.88 mm Hg in the phakic group（38 eyes） and 13.51±3.06 mm Hg in the pseudophakic group（38 eyes）（P〉0.05）.This changed little after SLT and IOP lowering effect was comparable between the two *** aim however was to lower the amount of medication *** the phakic group medication lowered from 1.29±0.62 at baseline,to 0.15±0.46 after 12mo;a reduction of 88.37%.In the pseudophakic group,used medication changed from 1.71±1.04,to 0.41±0.61;a 76.02% *** differences were not statistically significant at any time point（P〉0.05）.IOP lowering occurred slightly faster in thepseudophakic group（50% of patients after one week） than in the phakic group（68% of patients after more than 4wk）.The difference was not significant（P〉0.05）.CONCLUSION：IOP lowering effect of SLT is comparable between phakic and pseudophakic eyes.

关键词： mechanism of trabeculoplasty selective laser trabeculoplasty pseudophakes versus phakic eyes open angle glaucoma pressure lowering after lens extraction

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Negotiated Complexity: framing Multi-Criteria Decision Support in Environmental Health Practice

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American Journal of Operations Research 2013年第1期3卷 153-166页

作者： Hans Keune Johan Springael Wim De keyser faculty of applied economics University of Antwerp Antwerp Belgium Research Institute for Nature and forest (INBO) Brussels Belgium&Faculty of applied economics University of Antwerp Antwerp Belgium SIA Dendermonde Belgium

The complexity we take into account when dealing with complex issues and the way we deal with that complexity is not given or self-evident, it is framed and negotiated. Based on two environmental health decision support case studies we address a set of key methodological choices, crucial in shaping the multi-criteria decision support and illuminate how they followed from transdisciplinary collaboration and negotiation: diversity tolerance, dealing with uncertainty and difference of opinion, weight of information and the epistemological divide between traditional closed and alternative open paradigms. The case studies exemplify the growing conviction amongst methodologists that, especially regarding complex issues, best methods do not exist as such: methods are chosen and tailored in practice and the quality to a large extent is dependent on the process in which methodological development is embedded. We hope to contribute to making explicit the importance of methodological decision making regarding environmental health complexity.

关键词： Negotiated Complexity Multi-Criteria Decision Support Environmental Health Analytical Deliberative Approach Diversity Tolerance Traditional-Alternative Paradigms

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根据地面地震资料预测孔隙压力

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国外油气勘探 1992年第4期 89-95页

作者： Willam keyser 李秋成

掌握将要钻透地层的孔隙压力和与其有关的裂隙梯度,对于判定合适的钻井方案和安全钻井极为重要。钻井工程师得知了孔隙压力和裂隙梯度资料,就能够在钻进到某一深度时使用与地层孔隙压力相对应的泥浆比重,这样不仅提高了钻井速度和安全性... 详细信息

掌握将要钻透地层的孔隙压力和与其有关的裂隙梯度,对于判定合适的钻井方案和安全钻井极为重要。钻井工程师得知了孔隙压力和裂隙梯度资料,就能够在钻进到某一深度时使用与地层孔隙压力相对应的泥浆比重,这样不仅提高了钻井速度和安全性,而且对地层破坏最小。

关键词：地震数据孔隙压力压力预测裂隙带梯度

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Petterssen锋生函数的推广及其与垂直运动强迫项的关系

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气象科技 1989年第3期17卷 36-49页

作者： D.keyser 杨雪亚

Petterssen 的锋生方程,建立了水平位温梯度大小的拉格朗日变率(称为锋生函数)与水平速度场中运动学不变属性间的关系式。根据标量锋生函数的空间分布推断锋区中垂直环流的存在,在天气业务中是不罕见的。另一方面,Hoskins 及其同事引入... 详细信息

Petterssen 的锋生方程,建立了水平位温梯度大小的拉格朗日变率(称为锋生函数)与水平速度场中运动学不变属性间的关系式。根据标量锋生函数的空间分布推断锋区中垂直环流的存在,在天气业务中是不罕见的。另一方面,Hoskins 及其同事引入了一种准地转ω方程,其中动力强迫项与 Q 矢量的水平散度成正比。所谓 Q 矢量,定义为矢量水平位温梯度沿地转气流的拉格朗日变率。Q 矢量的形式,启发我们把 Petterssen 锋生函数推广成适用于矢量水平位温梯度的情况。这种推广后的锋生函数称为矢量锋生函数,包括新引入的水平位温梯度方向的拉格朗日变率表达式。

关键词：锋生函数 Petterssen 位温水平散度垂直运动变率锋区 ω方程强迫作用斜压扰动

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Identification and substrate prediction of new fragaria x ananassa aquaporins and expression in different tissues and during strawberry fruit development

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Horticulture Research 2018年第1期5卷 737-750页

作者： Britt Merlaen Ellen De keyser Marie-Christine Van Labeke Plant Production Faculty of Bioscience EngineeringGhent UniversityCoupure Links 6539000GentBelgium flanders Research Institute for Agriculture fisheries and food(ILVO) Plant Sciences UnitCaritasstraat 399090MelleBelgium

The newly identified aquaporin coding sequences presented here pave the way for further insights into the plant–water relations in the commercial strawberry(fragaria x ananassa).Aquaporins are water channel proteins that allow water to cross(intra)cellular *** fragaria x ananassa,few of them have been identified hitherto,hampering the exploration of the water transport regulation at cellular ***,we present new aquaporin coding sequences belonging to different subclasses:plasma membrane intrinsic proteins subtype 1 and subtype 2(PIP1 and PIP2)and tonoplast intrinsic proteins(TIP).The classification is based on phylogenetic analysis and is confirmed by the presence of conserved ***-specific signature sequences(SSSSs)and specificity-determining positions(SDPs)predict the substrate specificity of each new *** profiling in leaves,petioles and developing fruits reveals distinct patterns,even within the same(sub)*** profiles range from leaf-specific expression over constitutive expression to fruit-specific *** upregulation and downregulation during fruit ripening *** specificity and expression profiles suggest that functional specialization exists among aquaporins belonging to a different but also to the same(sub)class.

关键词： fragaria straw fir

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锋、急流和对流层顶的结构及动力学

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气象科技 1990年第2期18卷 7-11页

作者： M. A. Shapiro Daniel keyser 焦彦军国家海洋大气局/环境研究院波传播实验室美国纽约州立大学大气科学系

1.高层锋和急流二十世纪初期风筝等携带气象计的温度探测,首次使气象研究工作者观测到锋的垂直结构.Bjerknes和Palmén(1937)运用这些新观测论述了锋的"过渡带"结构,修正了早期的零阶不连续锋的概念.随着高空天气观测网空间分辨率的提高... 详细信息

1.高层锋和急流二十世纪初期风筝等携带气象计的温度探测,首次使气象研究工作者观测到锋的垂直结构.Bjerknes和Palmén(1937)运用这些新观测论述了锋的"过渡带"结构,修正了早期的零阶不连续锋的概念.随着高空天气观测网空间分辨率的提高,研究人员优化了他们的关于锋、急流和对流层顶的概念模式.

关键词：对流层顶地面锋锋区气象研究二十世纪初期次级环流降水过程过渡带粗虚线垂直结构

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