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Exploratory analysis of structural diversity indicators at stand level in three Italian beech sites and implications for sustainable forest management

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Journal of Forestry Research 2019年第1期30卷 121-127页

作者： Giada Bertini Claudia Becagli ugo chiavetta Fabrizio Ferretti Gianfranco Fabbio Luca Salvati CREA Research Centre for Forestry and Wood

The present study introduces an exploratory data analysis based on structural indicators with the aim to assess the effect of silvicultural practices on tree stand structure. The study was carried out in three Italian beech forests of different ages with stand structures that originated from dissimilar regeneration and cultivation techniques(Cansiglio, northern Italy, Chiarano, central Italy,and Mongiana, southern Italy). Ten structural indicators were considered when investigating the latent multivariate relationship between stand structure attributes before and after thinning operations by using a multiway factor analysis(MFA). The MFA results identified the older stand at Cansiglio as more homogeneous for cultivation regimes,and more stable to practices when compared with the younger sites(Chiarano and Mongiana). Heterogeneous stands were sensitive to silvicultural practice thus suggesting their possible impact on forest attributes. The proposed approach proved to be an operational tool to evaluate comprehensively the response of forest structure to planned interventions.

关键词： Forest diversity Multiway factor analysis Silviculture Structural diversity indicators Tree stand structure

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A Multiplex PCR-Based Next-Generation Sequencing Approach Has Detected a Common Large Deletion in STS Gene in a Patient with X-Linked Ichthyosis

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Journal of Biomedical Science and Engineering 2016年第7期9卷 337-341页

作者： Francesco Calì Giuseppa Maria Luana Mandarà Giuseppa Ruggeri Corrado Romano Valeria chiavetta Alda Ragalmuto Roberto Salluzzo Valentino Romano Marilena Galati Tardanico Carmelo Schepis Laboratory of Molecular Genetics Unit of Medical Genetics IRCCS Associazione Oasi Maria SS. Troina (EN) Italy Unit of Medical Genetics Azienda Sanitaria Provinciale di Ragusa Ragusa Italy Unit of Paediatrics and Medical Genetics IRCCS Associazione Oasi Maria SS. Troina (EN) Italy Department of Physics and Chemistry University of Palermo Palermo Italy Unit of Dermatology IRCCS Associazione Oasi Maria SS. Troina (EN) Italy

Several nuclear genes have been found to be linked to ichthyosis, and Next Generation Sequencing approach on panels of targeted genes has turned out to be particularly useful in analyzing diseases characterized by significant genetic and phenotypic heterogeneity. We developed a panel of 26 genes to be screened with the Ion Personal Genome Machine (PGM) for causative mutations relating to ichthyosis. Sequencing runs were obtained from a patient with ichthyosis using the Ion Torrent PGM and then processed with Ion Torrent Suite, Variant Caller, Coverage Analysis and wANNOVER tools. No causative mutations were found using Variant Caller and wANNOVER softwares, whereas the “Coverage Analysis” tool revealed a common large deletion in STS gene in a patient with X-linked ichthyosis. Identification of indels in Next Generation Sequencing (NGS) data is a veritable challenge. This study demonstrates the efficacy and effectiveness of using NGS approach to detect large deletions without resorting to specific algorithms for “indel” detection. Our results indicate that the NGS panel is a useful, rapid and cost-effective screening test for patients whose features are suggestive of a genetic etiology involving one of the genes embedded in the panel. It is an excellent alternative to Sanger sequencing as for costs, ease of analysis, and turnaround time.

关键词： X-Linked Ichthyosis STS Gene Next Generation Sequencing Coverage Analysis

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角膜植片上的“碎泥巴”样环丙沙星沉积物

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世界核心医学期刊文摘（眼科学分册） 2005年第9期1卷 46-47页

作者： Tanhehco T.Y. chiavetta III S.V. Lee P.P. et al. N.A. Afshari 杨建刚 Duke University Eye Center DUMC Box 3802 Durham NC 27710 United States Dr.

The authors describe an extreme case of ciprofloxacin deposition on the corneal graft of a 67-year-old man with a history of penetrating keratoplasty for corneal ulcer and perforation, who presented with dense, gray deposits on the anterior corneal surface after being treated with ciprofloxacin for an epithelial defect. The deposits resolved after ciprofloxacinwas discontinued. The non-physiologic pH of the drug formulation is thought to trigger corneal precipitates, and it remains to be seen whether fourth-generation fluoroquinoloneswill have a lower incidence of ocular precipitates.

关键词：角膜植片角膜溃疡穿孔非生理性上皮缺损喹诺酮类抗生素药物成分特殊病例

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