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Oestrous cycle of Wistar rats altered by sterol and triterpenes rich fraction of Adansonia digitata (Linn) root bark - A scientific rationale for contraceptive use

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Asian pacific Journal of Reproduction 2019年第2期8卷 75-82页

作者： Chibuogwu Ijeoma Chika Nwannenna Agnes Ifeyinwa ubah simon azubuike Ogwu David Department of Theriogenology and Production Ahmadu Bello UniversityZariaKaduna StateNigeria Department of Theriogenology Faculty of Veterinary MedicineUniversity of AbujaFederal Capital TerritoryNigeria

Objective: To investigate the effect of different fractions from the aqueous methanolic root bark of Adansonia digitata (Linn) on the estrous cycle of female Wistar rats and to elucidate their phytochemical content. Methods: Crude aqueous methanol extract of Adansonia digitata (Linn) root bark was fractionated by column chromatography using various ratios of hexane, acetyl acetate, methanol and distilled water. Fractions with similar trails on a thin layer chromatography were pooled into four fractions. Each fraction was then screened for its phytochemical content. Forty mature Wistar female rats weighing (135±28) g with regular estrous cycles were given each fraction at two dose levels while a control group received distilled water of equal volume. Treatments were given per os for 15 days starting from the day of estrus. Vaginal smears were observed daily for the duration of each cycle, frequency of occurrence of each stage of the estrous cycle and number of cycles for 15 days before and during treatment. At the end of treatment, rats were humanely sacrificed. Ovaries and uteri were weighed and processed for histopathology. Results: Hexane acetyl acetate and acetyl acetate methanol fractions at 150 mg/kg body weight and 300 mg/kg body weight prolonged the occurrence of proestrus and diestrus and produced atretic cyst-like ovaries at 300 mg/kg body weight. Acetyl acetate methanol treated rats also had low relative ovarian weights when compared to the control. Conclusions: Sterols and triterpenes in hexane acetyl acetate and acetyl acetate methanol fractions of the root bark may be responsible for the disruption of estrous cycle in female Wistar rats and may have a contraceptive effect.

关键词： Proestrus Diestrus Adansonia digitata (Linn) Root bark Contraception

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Pregnancy Rate of Bunaji Cows in a Fixed Time AI Synchronization Protocol Using Ovatide

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Journal of Agricultural Science and Technology(A) 2019年第1期9卷 46-55页

作者： ubah simon azubuike Rekwot Peter Ibrahim Adewuyi Abdulmujeeb Bode Omontese Bobwealth Oakina Adeyeye Adewale Ayodeji Columbus Philemon Kwinjoh Abah Owoicho Kenneth Agbonu Oluwa Adikpe Ejiofor Charles Ejike Department of Theriogenology University of AbujaNigeria Artificial Insemination Unit National Animal Production Research InstituteShikaZariaKaduna StateNigeria Department of Theriogenology and Animal Production Usmanu Danfodiyo UniversitySokotoNigeria Department of Veterinary Physiology and Biochemistry University of AbujaNigeria Department of Veterinary Parasitology University of AbujaNigeria

This study accessed the reproductive performance of Bunaji cows in an Ovsynch protocol involving ovatide.Bunaji cows(n=16)aged 4-6 years and weighing between 250-350 kg with body condition scores(BCS)of 2.5-3.5 were used.There were two treatment groups for synchronization of ovulation.Treatment group 1 comprising Bunaji(n=8)received 50μg of gonadotropin releasing hormone(GnRH)(Cystorelin)and 25 mg of PGF2α.While,treatment group 2 comprising Bunaji(n=8)received 50μg of ovatide and 25 mg of PGF2α.All cows from both treatment groups were inseminated at 16 h after each second GnRH or ovatide injections.Cows that showed mucus discharge from the vagina on the day of artificial insemination(AI)were recorded as well as those that had patent cervix.Cervical dilation was measured by taking note of the cows that had mid cervix insemination due to non-passage of the AI gun through the cervix.Transrectal palpation was conducted twice at a month interval to select cycling cows within 5-12 d of the estrous cycle before initiating Ovsynch protocol.It was repeated on day 45 post AI to confirm pregnancies in animals.Results showed that the rate of mucus discharge from the vagina was 37.5%and 87.5%for ovatide and Cystorelin,respectively;while the rate of cervical dilation were 75%and 87.5%for ovatide and Cystorelin,respectively(p>0.05),and pregnancy rates were 0%and 12.5%for ovatide and Cystorelin,respectively.There were no significant differences between the two groups.It was concluded that treatment of Bunaji cows with 50μg ovatide in Ovsynh protocol has heat and ovulation synchronization potentials and zero pregnancy rate.It was recommended that further studies be carried out using graded doses of 50,100 and 150μg of ovatide in a fixed time AI synchronization protocol in Bunaji cows,to tap the potentials of the hormone in manipulation of bovine reproduction.

关键词： Pregnancy ovatide fixed time AI synchronization cows

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