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Qu-2,a robust poplar suspension cell line for molecular biology

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Journal of Forestry Research 2021年第2期32卷 733-740页

作者： Caixia Liu Kailong Li Meng wang Erqin Fan Chuanping Yang Junhui wang Pengyue Fu Xiaolan Ge Heike W.Sederoff Ronald RSederoff Vincent Lchiang Sui wang Guanzheng Qu State Key Laboratory of Tree Genetics and Breeding Northeast Forestry UniversityHarbin 150040People’s Republic of China State Key Laboratory of Tree Genetics and Breeding Key Laboratory of Tree Breeding and Cultivation of State Forestry AdministrationResearch Institute of ForestryChinese Academy of ForestryBeijing 100091People’s Republic of China Department of Plant and Microbial Biology North Carolina State UniversityRaleighNC 27695USA Forest Biotechnology Group Department of Forestry and Environmental ResourcesNorth Carolina State UniversityRaleighNC 27695USA Key Laboratory of Soybean Biology in Chinese Ministry of Education Northeast Agricultural UniversityHarbin 150030People’s Republic of China

Populus *** long been used as model woody plant species for molecular biology ***,tissues of poplar are often recalcitrant to experimental procedures for molecular *** generated a hormone autotrophic poplar suspension cell line from a hybrid of Populus alba×***‘Yinzhong’,named ***-2 cells are suitable as a model biological system for studying woody ***-2 cells have many advantages over suspension cell lines derived so far from any other woody ***-2 cells are very easy to cultivate and can grow on several common plant culture media without the addition of any plant *** show exceptionally high growth rates,reaching an approximately 150-fold increase in biomass after one week of *** important unique characteristic of Qu-2 cells is that they can be cryopreserved and readily ***-2 cells are suitable for molecular manipulations such as protoplast production,transient transformation,and RNA-seq ***,Qu-2 cells have the great potential to be an excellent model cell line in tree molecular biological research,ranging from physiology to gene *** Qu-2 cells will be made available to the plant community for research.

关键词： Qu-2 cell line Suspension cell Poplar Protoplast isolation Transient transformation

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局部低频振动刺激对手部运动功能的瞬时影响

医用生物力学 2011年第4期26卷 310-314页

作者：刘强杨雯雯王子玮陈全寿相子元台北市立体育学院台北11100 台湾体育学院台中40404 台湾师范大学台北11677

目的探讨局部低频振动刺激对手部控制能力、柔韧性、握力及肌肉兴奋程度的瞬时影响。方法 10位受试者以最大等长收缩握住手部振动器20 s,在此过程中随机给予12.5 Hz振动频率(振动组)或无振动刺激(对照组)。在介入前后分别进行手部控制... 详细信息

目的探讨局部低频振动刺激对手部控制能力、柔韧性、握力及肌肉兴奋程度的瞬时影响。方法 10位受试者以最大等长收缩握住手部振动器20 s,在此过程中随机给予12.5 Hz振动频率(振动组)或无振动刺激(对照组)。在介入前后分别进行手部控制能力、柔韧性、握力及手部肌肉兴奋程度等测验,并采用配对t检验比较前后测试的差异和各组变化率。结果组内比较发现振动组在控制能力与柔韧性表现上进步显著(P<0.05),对照组在握力与肌肉活化上出现显著下降(P<0.05);组间变化率比较结果显示振动组在握力与肌肉兴奋程度变化率皆显著大于对照组(P<0.05)。结论局部低频振动刺激可瞬时提升手部控制能力与柔韧性表现,未来可运用于各种需要手部执行工作的相关人员。

关键词：低频振动刺激控制能力肌肉肌电信号生物力学

TWO CASES OF APPLICATION OF THE METHOD OF CONSTRUCTION BY CONSTANTS IN LATTICE-VALUED MODEL THEORY

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Chinese Science Bulletin 1981年第7期26卷 581-584页

作者：王世强 but the content discussed is independent of it. The main content of this paper is two cases of application of the method of constructing models by constants. The first one proves the compactness theorem of certain

This paper is a continuation of the work begun in [2], which is to generalize the theory of the two-valued models to lattice-valued ones. The basic conceptions and the symbols used in this paper are the same as those in [2], but the content discussed is independent of it. The main content of this paper is two cases of application of the method of constructing models by constants. The first one proves the compactness theorem of

关键词： continuation valued generalize compactness constants constructing proof partition subset generalization

Efficient expression of histidine-tagged large hepatitis delta antigen in baculovirus-transduced baby hamster kidney cells

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World Journal of Gastroenterology 2006年第10期12卷 1551-1557页

作者： Ying-Wei chiang Jaw-Chin Wu Kuei-Chun wang Chia-Wei Lai Yao-Chi Chung Yu-Chen Hu Department of Chemical Engineering National Tsing Hua University Hsinchu 300 Taiwan China Institute of Clinical Medicine National Yang Ming University and Division of Gastroenterology Department of Medicine Taipei Veterans General Hospital Taipei 112 Taiwan China

AIM： To study the baculovirus/mammalian cell system for efficient expression of functional large hepatitis delta antigen （L-HDAg）. METHODS： A recombinant baculovirus expressing histidine-tagged L-HDAg （L-HDAgH） was constructed to transduce baby hamster kidney （BHK） cells by a simplified transduction protocol. RESULTS： The recombinant baculovirus transduced BHK cells with efficiencies higher than 90% as determined by flow cytometry. The expression level was significantly higher than that obtained by plasmid transfection and was further enhanced 3-fold to around 19 pg/cell by the addition of 10 mmol/L sodium butyrate. Importantly, the expressed L-HDAgH was localized to the cell nucleus and correctly isoprenylated as determined by immunofluorescence labeling and confocal microscopy. Moreover, L-HDAgH interacted with hepatitis B surface antigen to form virus-like particles. CONCLUSION： The fusion with histidine tags as well as overexpression of L-HDAgH in the baculovirus-transduced BHK cells does not impair the biological functions. Taken together, the baculovirus/mammalian cell system offers an attractive alternative for high level expression of L-HDAgH or other proteins that require extensive posttranslational modifications.

关键词： Baculovirus Hepatitis delta virus L-HDAg Mammalian cell Protein expression Transduction

Tanshinone IIA Induces Apoptosis of Cervical Cancer HeLa Cells and Advanced CaSki Cell with Proteomic Study

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Tanshinone IIA Induces Apoptosis of Cervical Cancer HeLa Cel...

Approved pharmacotherapy for myopic choroidal neovascularization:a review of randomized controlled trials in ranibizumab and aflibercept

第十四届全国中药和天然药物学术研讨会

作者： Yu-chiang Hung Tai-Long Pan Pei-Wen wang Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine

Objective Cervical cancer is one of the most common causes of mortality to females in the *** present,paclitaxel(taxol)is the first line drug used to therapy of cervical ***,administration of chemotherapy such as paclitaxel usually results in drug resistance and shows no significant effect on advanced cervical *** purpose of the present study is to confirm our hypothesis that tanshinone *** from the root of Salvia miltiorrhiza could effectively inhibit cervical *** would like to found the anti-cancer effects of tanshinone IIA on cervical cancer HeLa cells or advanced CaSki cells with invasive potential and investigate the underlying mechanisms through functional *** hope to find a better complementary alternative drug for cervical *** Cell Culture and MTT assay,flow cytometric analysis,DNA fragmentation assay,western blot analysis,2-DE and image analysis,tryptic in-gel enzymatic digestion and MALDI-TOF/TOF MS analysis,biological network analysis using MetaCorewere used to found the c mechanism of *** Our results indicated that tanshinone HA could inhibit HeLa cells and CaSki cell *** induce apoptosis through activating p53 expression and regulating mitochondrial intrinsic and endoplasmic reticulum stress *** cytometric analysis of cell cycle progression revealed that G2/M arrest was initiated after a 24-h exposure to the *** also resulted in DNA fragmentation and degradation of poly(ADP-ribose)*** addition,changes in expression levels of several protein targets have been identified under tanshinone IIA *** Tanshinone IIA enhancing apoptosis of cervical cancer HeLa cells and advanced CaSki cell through activating p53 expression and regulating mitochondria intrinsic and endoplasmic reticulum stress pathways could be as a complementary alternative therapy of cervical cancer.

关键词： Tanshinone IIA HeLa cell Caski cell cervical cancer

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Eye Science 2015年第4期30卷 198-200页

作者： Jia-Kang wang tzu-Lun Huang Pei-Yuan Su Pei-Yao Chang Department of Ophthalmology Far Eastern Memorial Hospital Department of Medicine National Yang Ming University Department of Healthcare Administration Oriental Institute of Technology Department of Nursing Oriental Institute of Technology Institute of Medical Sciences Tzu Chi University Department of Medicine National Taiwan University Department of Medicine Fu Jen Catholic University

Myopic choroidal neovascularization(mCNV) can cause severe visual impairment in highly myopic patients. We review the randomized trials of two approved pharmacotherapy for treating mCNV, including intravitreal injections of ranibizumab and aflibercept. These two vascular endothelial growth factor(VEGF) antagonists show superior ability to improve vision and reduce macular thickness, comparing with sham injections or verteporfin photodynamic therapy(vPDT). There is no severe ocular or systemic adverse reaction reported in studies associated with ranibizumab and aflibercept for mCNV. Prompt treatment with these agents can lead to a better outcome.

关键词：药物治疗新生血管随机试验脉络膜单抗临床试验近视血管内皮生长因子

台湾幼托整合后学前教育之创新——台湾幼儿园教保活动课程新课纲解读

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湛江师范学院学报 2014年第1期35卷 5-11页

作者：蒋姿仪台中教育大学幼儿教育学系台湾台中40306

为确保健全幼儿教保环境的提供,符合"提供一致教保服务"之国际趋势,推动台湾幼教改革符合时代环境的需要,台湾学前幼儿教保政策历经14年的推动之后终于形成并实施"幼儿教育及照顾法",使台湾的学前教育从此迈入一个崭新的时代,并因应幼... 详细信息

为确保健全幼儿教保环境的提供,符合"提供一致教保服务"之国际趋势,推动台湾幼教改革符合时代环境的需要,台湾学前幼儿教保政策历经14年的推动之后终于形成并实施"幼儿教育及照顾法",使台湾的学前教育从此迈入一个崭新的时代,并因应幼托整合,制定幼儿园教保活动新课纲。新课纲强调课程应以统整的方式实施,并依据幼儿的需求与社会的期待,将其内涵分为身体动作与健康、认知、语文、社会、情绪和美感等六个领域。新课纲要求教保活动课程基于统整课程理念设计,培养与陶冶幼儿拥有觉知辨识、表达沟通、关怀合作、推理赏析、想象创造与自主管理等六大能力。幼托整合是台湾幼儿教育发展过程中一个重要且具时代意义的变革。

关键词：幼托整合幼儿教育统整课程

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《淮南子》的文化理念研究

淮南师范学院学报 2024年第3期26卷 29-32页

作者：王静博中共淮南市委党校马克思主义基本理论教研室安徽淮南232001

中华优秀传统文化是中华民族优秀的文化基因,是中华民族传承发展的精神动力。《淮南子》作为中华名史典籍,是中华优秀传统文化的一部分,蕴含着可供借鉴的文化理念。比较突出的有“利民为本”的民本思想、“法与时变”的创新意识、“德... 详细信息

中华优秀传统文化是中华民族优秀的文化基因,是中华民族传承发展的精神动力。《淮南子》作为中华名史典籍,是中华优秀传统文化的一部分,蕴含着可供借鉴的文化理念。比较突出的有“利民为本”的民本思想、“法与时变”的创新意识、“德法并举”的理政观念,以及“人亦物也”的生命共同体理念等,挖掘其中蕴藏的古代智慧对当前推进中国式现代化建设具有积极意义。

关键词：中华优秀传统文化《淮南子》文化理念

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大尺度有效引力的E(2)规范理论模型

物理学报 2017年第13期66卷 13-21页

作者：魏文叶申佳音吴奕暐杨礼想薛迅阮自强华东师范大学物理系上海200241 中央研究院物理研究所台北11529

微波背景辐射的低l极矩的各向异性可能不能用微波背景辐射静止系boost到本动参考系来解释,我们推断boost对称性在宇宙学尺度上缺失,又由于单纯结合广义相对论和物质结构的标准模型不能解释星系以上尺度的引力现象,需要引入暗物质和暗能... 详细信息

微波背景辐射的低l极矩的各向异性可能不能用微波背景辐射静止系boost到本动参考系来解释,我们推断boost对称性在宇宙学尺度上缺失,又由于单纯结合广义相对论和物质结构的标准模型不能解释星系以上尺度的引力现象,需要引入暗物质和暗能量.而迄今为止所有寻找暗物质粒子的实验给出的都是否定结果,暗能量的本质更是一个谜.因此,我们假设洛伦兹对称性是从星系以上尺度开始部分破缺,以非常狭义相对论对称群E(2)为例,用E(2)规范理论来构造大尺度有效引力理论,并分析了此规范理论的自洽性.从这些讨论中发现,当物质源即使为普通标量物质时,contortion也一般非零,非零contortion的存在会贡献一个等效能量动量张量的分布,它可能对暗物质效应给出至少部分的贡献.我们从对称性出发修改引力,有别于其他的修改引力理论.

关键词： E(2)群非常狭义相对论有效引力理论洛伦兹破缺