Background:Ocimum americanum *** an annual herbaceous plant used to manage pain and *** of standard dosage forms,and safety and efficacy data potentially exposes users of this medicinal plant to the risk of toxic or s...
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Background:Ocimum americanum *** an annual herbaceous plant used to manage pain and *** of standard dosage forms,and safety and efficacy data potentially exposes users of this medicinal plant to the risk of toxic or sub-therapeutic effects,as well as drug-herb ***:The study developed a standard dosage form containing *** extract and assessed its anti-inflammatory efficacy and ***:The fusion method was used to formulate an ointment comprising acetone extract,beeswax,and *** optimization involved the application of Box-Behnken Design(BBD)to design an exper-iment whose input variables were%beeswax and%soft-paraffin and its melting *** variables were drug release and viscosity which were quantified using a vertical diffusion cell(VDC)and stanhope-seta viscometer,*** albino rats were used in carrageenan-induced rat hind paw edema(n=6/test,positive and negative control)to assess the anti-inflammatory *** studies comprised assessment of spreadability,thin layer chromatograms(TLC),and microbial growth in ointment batches stored at 25±2℃/60±5%RH and 40±2℃/75±5%***:Temperature and%beeswax significantly influenced drug release(P=0.0024).Optimal drug release and viscosity were obtained at 5.4%beeswax,89.6%soft-paraffin melted at 58℃.The predominant drug release mechanism was Higuchi(R^(2)=0.967±0.023),which implied longer contact between formulation and skin favours drug *** of edema by the ointment was comparable to 1%(w/w)diclofenac gel(t=2.1;CI=(-8.29)-1.15),and both differed significantly with negative control(t=11.3 and 13.4;CI=14.5-23.9 and 18.1-27.5 respectively).Insignificant changes in spreadability(P=0.112),absence of new spots on chromatograms,and deficiency of microbial colonies on agar plates implied physical,chemical,and microbial stability,***:A formulated ointment containing *** extract ex
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