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Visual detection of tropomyosin,a major shrimp allergenic protein using gold nanoparticles(AuNPs)-assisted colorimetric aptasensor

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Marine Life Science & Technology 2021年第3期3卷 382-394页

作者： tushar Ramesh Pavase Hong Lin Maqsood Ahmed Soomro Hongwei Zheng Xiaxia Li Kexin Wang Zhenxing Li Food Safety Laboratory Department of Food Science and EngineeringCollege of Food Science and EngineeringOcean University of ChinaQingdao266003China Fish Molecular Immunology Laboratory College of Marine Life SciencesOcean University of ChinaQingdao266003China

A gold nanoparticle-based label-free colorimetric assay was developed to detect the shrimp allergenic protein tropomyosin(TM),an important biomarker responsible for severe clinical reactivity to *** a gold nanoparticles(AuNPs)-tropomyosin-binding aptamer(TMBA)complex,the aptamer adsorbs onto the surface of AuNPs and dissociates in the presence of *** addition,AuNPs tend to aggregate in the presence of ionic salt,revealing a color change(i.e.,wine-red to purple/blue)with a shift in the maximum absorption peak from 520 *** the presence of specific binding TM,the aptamer folds into a tertiary structure where it more efficiently stabilizes AuNPs toward the salt-induced aggregation with a hypsochromic shift in the absorption spectra compared to the stabilized AuNPs by aptamer *** on the aggregation and sensitive spectral transformation principle,the AuNPs-based colorimetric aptasensor was successfully applied to detect TM with a range of 10-200 nmol/L and a low detection limit of 40 nmol/L in water *** reliability,selectivity,and sensitivity of the aptasensor was then tested with food samples spiked with *** observed detection limit was as low as 70 nmol/L in shrimp,90 nmol/L in tofu,and 80 nmol/L in eggs,*** anticipate the proposed AuNPs-based colorimetric aptasensor assay possesses a high potential for the easy and efficient visual colorimetric detection of TM.

关键词： Gold nanoparticles(AuNPs) Aptamer Aggregation Colorimetric assay Shellfish allergenic protein Shrimp tropomyosin(TM)

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Programmable microfluidic genotyping of plant DNA samples for marker-assisted selection

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Microsystems & Nanoengineering 2018年第1期4卷 413-422页

作者： Helena C.Zec Tony Zheng Lingshu Liu Kuangwen Hsieh tushar D.Rane Todd Pederson Tza-Huei Wang Department of Biomedical Engineering Johns Hopkins UniversityBaltimoreMD 21218USA Department of Mechanical Engineering Johns Hopkins UniversityBaltimoreMD 21218USA Pioneer Hi-Bred International Inc. JohnstonIA 50131USA

As demands to maintain the global food production continue to mount,multinational seed companies are turning to new DNA marker technologies to accelerate the rate of plant breeding and crop *** key to widespread adoption of molecular breeding is the availability of flexible and cost-effective tools that can perform combinatorial and high-throughput genotyping of single-nucleotide polymorphisms(SNPs)to guide the crop development *** this end,we have developed a programmable,droplet-based microfluidic device for genotyping maize genomic DNA.A unique feature of the microfluidic platform is the nano sample processors(NSPs),which allow the device to sequentially load an unrestricted number of unique DNA samples using only two inlets,overcoming the current limitation to the number of sample inputs due to small device *** and programmable droplet generation within the device allows each sample to be genotyped against a panel of markers on ***,we have successfully implemented the Invader assay for SNP genotyping in flowing,50-nL droplets,thus achieving significant reduction in consumption of reagents per reaction as compared with conventional genotyping *** a demonstration,we performed 240 Invader reactions(testing 8 DNA samples against 10 SNP markers)and achieved greater than 93% accuracy in SNP calling of plant DNA samples in a single droplet-based experiment.

关键词： droplets genotyping invader assay microfluidics single-nucleotide polymorphism

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Malnutrition:laboratory markers vs nutritional assessment

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Gastroenterology Report 2016年第4期4卷 272-280,I0001页

作者： Shishira Bharadwaj Shaiva Ginoya Parul Tandon tushar D.Gohel John Guirguis Hiren Vallabh Andrea Jevenn Ibrahim Hanouneh Department of Gastroenterology/Hepatology McMaster UniversityHamiltonOntarioCanada Department of Gastroenterology/Hepatology Cleveland ClinicClevelandOHUSA

Malnutrition is an independent risk factor for patient morbidity and mortality and is associated with increased healthcarerelated ***,a major dilemma exists due to lack of a unified definition for the ***,there are no standard methods for screening and diagnosing patients with malnutrition,leading to confusion and varying practices among physicians across the *** role of inflammation as a risk factor for malnutrition has also been recently ***,serum proteins such as albumin and prealbumin(PAB)have been widely used by physicians to determine patient nutritional ***,recent focus has been on an appropriate nutrition-focused physical examination(NFPE)for diagnosing *** current consensus is that laboratory markers are not reliable by themselves but could be used as a complement to a thorough physical *** studies are needed to identify serum biomarkers in order to diagnose malnutrition unaffected by inflammatory states and have the advantage of being noninvasive and relatively ***,a thorough NFPE has an unprecedented role in diagnosing malnutrition.

关键词： malnutrition physical examination serum markers

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A Novel Machine Learning-Based Hand Gesture Recognition Using HCI on IoT Assisted Cloud Platform

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Computer Systems Science & Engineering 2023年第8期46卷 2123-2140页

作者： Saurabh Adhikari tushar Kanti Gangopadhayay Souvik Pal D.Akila Mamoona Humayun Majed Alfayad N.Z.Jhanjhi School of Engineering Swami Vivekananda UniversityIndia Department of Computer Science and Engineering Sister Nivedita University(Techno India Group)KolkataWest BengalIndia Sambalpur University SambalpurIndia Department of Computer Applications Saveetha College of Liberal Arts and SciencesSIMATS Deemed to be UniversityChennaiIndia Department of Information Systems College of Computer and Information SciencesJouf UniversityKSA College of Computer and Information Sciences Jouf UniversitySakaka72341Saudi Arabia School of Computer Science(SCS) Taylor’s UniversitySubang Jaya47500Malaysia

Machine learning is a technique for analyzing data that aids the construction of mathematical *** of the growth of the Internet of Things(IoT)and wearable sensor devices,gesture interfaces are becoming a more natural and expedient human-machine interaction *** type of artificial intelligence that requires minimal or no direct human intervention in decision-making is predicated on the ability of intelligent systems to self-train and detect *** rise of touch-free applications and the number of deaf people have increased the significance of hand gesture *** applications of hand gesture recognition research span from online gaming to surgical *** location of the hands,the alignment of the fingers,and the hand-to-body posture are the fundamental components of hierarchical emotions in *** gestures may be difficult to distinguish from nonsensical motions in the field of gesture *** gestures may be difficult to distinguish from nonsensical motions in the field of gesture *** this scenario,it may be difficult to overcome segmentation uncertainty caused by accidental hand motions or *** a user performs the same dynamic gesture,the hand shapes and speeds of each user,as well as those often generated by the same user,vary.A machine-learning-based Gesture Recognition Framework(ML-GRF)for recognizing the beginning and end of a gesture sequence in a continuous stream of data is suggested to solve the problem of distinguishing between meaningful dynamic gestures and scattered *** have recommended using a similarity matching-based gesture classification approach to reduce the overall computing cost associated with identifying actions,and we have shown how an efficient feature extraction method can be used to reduce the thousands of single gesture information to four binary digit gesture *** findings from the simulation support the accuracy,precision,gesture

关键词： Machine learning gesture recognition framework accuracy rate precision rate gesture recognition rate sensitivity rate efficiency rate

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The expression of proteins involved in digestion and detoxification are regulated in Helicoverpa armigera to cope up with chlorpyrifos insecticide

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Insect Science 2016年第1期23卷 68-77页

作者： Vishal V. Dawkar Yojana R. Chikate tushar H. More Vidya S. Gupta Ashok P. Giri Plant Molecular Biology Unit Division of Biochemical Sciences CSIR-National Chemical Laboratory Dr. Homi Bhabha Road Pun e 411008(MS) India

Helicoverpa armigera is a key pest in many vital crops, which is mainly controlled by chemical strategies, To manage this pest is becoming challenging due to its ability and evolution of resistance against insecticides. Further, its subsequent spread on nonhost plant is remarkable in recent times. Hence, decoding resistance mechanism against phytochemicals and synthetic insecticides is a major challenge. The present work describes that the digestion, defense and immunity related enzymes are associated with chlorpyrifos resistance in 11. armigera. Proteomic analysis of/-/, armigera gut tissue upon feeding on chlorpyrifos containing diet （CH） and artificial diet （AD） using nano-liquid chromatography-mass spectrometry identified upregulated 23-proteins in CH fed larvae. Database searches combined with gene ontology analysis revealed that the identified gut proteins engrossed in digestion, proteins crucial for immunity, adaptive responses to stress, and detoxification. Biochemical and quantitative real-time polymerase chain reaction anal- ysis of candidate proteins indicated that insects were struggling to get nutrients and energy in presence of CH, while at the same time endeavoring to metabolize chlorpyrifos. More- over, we proposed a potential processing pathway of chlorpyrifos in H. armigera gut by examining the metabolites using gas chromatography-mass spectrometry. 1f. armigera exhibit a range of intriguing behavioral, morphological adaptations and resistance to in- secticides by regulating expression of proteins involved in digestion and detoxification mechanisms to cope up with chlorpyrifos. In these contexts, as gut is a rich repository of biological information; profound analysis of gut tissues can give clues of detoxification and resistance mechanism in insects.

关键词： chlorpyrifos Helicoverpa armigera insecticide resistance proteomics real-time PCR

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Percutaneous direct endoscopic pancreatic necrosectomy

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World Journal of Gastrointestinal Surgery 2022年第8期14卷 731-742页

作者： Manoj A Vyawahare Sushant Gulghane Rajkumar Titarmare tushar Bawankar Prashant Mudaliar Rahul Naikwade Jayesh M Timane Department of Medical Gastroenterology American Oncology Institute at Nangia Specialty HospitalNagpur 440028MaharashtraIndia Department of Internal Medicine and Critical Care American Oncology Institute at Nangia Specialty HospitalMIDC HingnaNagpur 440028MaharashtraIndia Department of Anaesthesiology American Oncology Institute at Nangia Specialty HospitalMIDC HingnaNagpur 440028MaharashtraIndia Department of Radiology American Oncology Institute at Nangia Specialty HospitalMIDC HingnaNagpur 440028MaharashtraIndia Department of Surgery American Oncology Institute at Nangia Specialty HospitalMIDC HingnaNagpur 440028MaharashtraIndia

Approximately 10%-20% of the cases of acute pancreatitis have acute necrotizing pancreatitis. The infection of pancreatic necrosis is typically associated with a prolonged course and poor prognosis. The multidisciplinary, minimally invasive “step-up” approach is the cornerstone of the management of infected pancreatic necrosis(IPN). Endosonography-guided transmural drainage and debridement is the preferred and minimally invasive technique for those with IPN. However, it is technically not feasible in patients with early pancreatic/peripancreatic fluid collections(PFC)(< 2-4 wk) where the wall has not formed;in PFC in paracolic gutters/pelvis;or in walled off pancreatic necrosis(WOPN) distant from the stomach/duodenum. Percutaneous drainage of these infected PFC or WOPN provides rapid infection control and patient stabilization. In a subset of patients where sepsis persists and necrosectomy is needed, the sinus drain tract between WOPN and skin-established after percutaneous drainage or surgical necrosectomy drain, can be used for percutaneous direct endoscopic necrosectomy(PDEN). There have been technical advances in PDEN over the last two *** esophageal fully covered self-expandable metal stent, like the lumen-apposing metal stent used in transmural direct endoscopic necrosectomy, keeps the drainage tract patent and allows easy and multiple passes of the flexible endoscope while performing PDEN. There are several advantages to the PDEN procedure. In expert hands, PDEN appears to be an effective, safe, and minimally invasive adjunct to the management of IPN and may particularly be considered when a conventional drain is in situ by virtue of previous percutaneous or surgical intervention. In this current review, we summarize the indications, techniques, advantages, and disadvantages of PDEN. In addition, we describe two cases of PDEN in distinct clinical situations, followed by a review of the most recent literature.

关键词： Infected pancreatic necrosis Direct endoscopic necrosectomy Percutaneous endoscopic necrosectomy Sinus tract endoscopy Stent-assisted percutaneous direct endoscopic necrosectomy

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FPGA所需的电源供应：深入分析

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电子技术应用 2006年第7期32卷 I0002-I0009页

作者： tushar Dhayagude 美国国家半导体电源管理产品部市场经理

无论是网络产品、通信设备、工业系统，还是汽车电子系统，这些电子系统都普遍采用现场可编程门阵列fFPGA）芯片，显示这种芯片在实际应用时具有高度的灵活性，加上FPGA具有可重新配置的高度智能，因此轻易成为上述各种电子产品所不可... 详细信息

无论是网络产品、通信设备、工业系统，还是汽车电子系统，这些电子系统都普遍采用现场可编程门阵列fFPGA）芯片，显示这种芯片在实际应用时具有高度的灵活性，加上FPGA具有可重新配置的高度智能，因此轻易成为上述各种电子产品所不可缺少的基本元件。最近，FPGA芯片的应用范围进一步扩大，甚至广泛应用各种电子消费产品，例如机顶盒、DVD录像机及电子游戏机。预计FPGA芯片的应用范围会进一步扩大，但相信在全球定位系统、医疗设备、测量仪表以及电子消费产品等便携式设备会有进一步的增长。

关键词： FPGA芯片电源供应汽车电子系统现场可编程门阵列网络产品 DVD录像机全球定位系统电子游戏机便携式设备通信设备

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Adsorption Characteristics of Zinc (Zn<sup>2+</sup>) from Aqueous Solution by Natural Bentonite and Kaolin Clay Minerals: A Comparative Study

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Computational Water, Energy, and Environmental Engineering 2013年第3期2卷 1-6页

作者： tushar Kanti Sen Chi Khoo Department of Chemical Engineering GPO Box U1987 Curtin University Perth 6845 Western Australia Australia

Clay minerals are one of the potential good adsorbent alternatives to activated carbon because of their large surface area and high cation exchange capacity. In this work the adsorptive properties of natural bentonite and kaolin clay minerals in the removal of zinc (Zn2+) from aqueous solution have been studied by laboratory batch adsorption kinetic and equi- librium experiments. The result shows that the amount of adsorption of zinc metal ion increases with initial metal ion concentration, contact time, but decreases with the amount of adsorbent and temperature of the system for both the ad- sorbents. Kinetic experiments clearly indicate that adsorption of zinc metal ion (Zn2+) on bentonite and kaolin is a two-step process: a very rapid adsorption of zinc metal ion to the external surface is followed by possible slow decreas- ing intraparticle diffusion in the interior of the adsorbent. This has also been confirmed by an intraparticle diffusion model. The equilibrium adsorption results are fitted better with the Langmuir isotherm compared to the Freundlich model. The value of separation factor, RL from Langmuir equation give an indication of favourable adsorption. Finally from thermodynamic studies, it has been found that the adsorption process is exothermic due to negative ?H0 accompa- nied by decrease in entropy change and Gibbs free energy change (?G0). Overall bentonite is a better adsorbent than kaolin in the the removal of Zn2+ from its aqueous solution.

关键词： Metal Ion Adsorption Clay Minerals Kinetics Isotherms

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Patient prompting of their physician resulted in increased colon cancer screening referrals

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World Journal of Gastrointestinal Oncology 2014年第7期6卷 257-262页

作者： Vu Le Saqib Syed Kenneth J Vega tushar Sharma Mohammad F Madhoun Nakumar Srinivasan Courtney W Houchen Division of Digestive Disease and Nutrition Univer-sity of Oklahoma Health Sciences Center Oklahoma City OK 73104 United States College of Public Health University of Okla-homa Health Sciences Center Oklahoma City OK 73104 United States

AIM:To determine whether a communication instru-ment provided to patients prior to their primary carephysician(PCP)visit initiates a conversation with theirPCP about colorectal cancer screening(CRC-S),impact-ing screening referral rates in fully insured and underin-sured ***:A prospective randomized control studywas performed at a single academic center outpatient internal medicine(IRMC,underinsured)and fam-ily medicine(FMRC,insured)resident clinics prior to scheduled *** the intervention group,a pamphlet about the benefit of CRC-S and a reminder card were given to patients before the scheduled visit for prompt-ing of CRC-S referral by their *** main outcome measured was frequency of CRC-S referral in each clinic after ***:In the IRMC,148 patients participated,a control group of 72 patients(40F and 32M)and 76 patients(48F and 28M)in the intervention ***-ferrals for CRC-S occurred in 45/72(63%)of control vs 70/76(92%)in the intervention group(P≤0.001).In the FMRC,126 patients participated,66(39F:27M)con-trol and 60(33F:27M)in the intervention ***-S referrals occurred in 47/66(71%)of controls vs 56/60(98%)in the intervention group(P≤0.001).CONCLUSION:Patient initiated physician prompting produced a significant referral increase for CRC-S in un-derinsured and insured patient *** investigation aimed at increasing CRC-S acceptance is warranted.

关键词： Colon cancer Screening Primary care Physician patient relationship Referral

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Performance Evaluation of Antenna Receive Diversity in SlMO-OFDM System over Rayleigh Fading Channel

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Computer Technology and Application 2013年第7期4卷 364-373页

作者： Ripan Kumar Roy tushar Kanti Roy Department of ETE Faculty of ECE Rajshahi University of Engineering & Technology (RUET) Rajshahi 6204 Bangladesh

This paper represents a comparative performance evaluation of different diversity combining techniques for a SIMO-OFDM （single-input-multiple-output orthogonal frequency division multiplexing） system over Rayleigh fading channel. OFDM is a key technique for achieving high data rates and spectral efficiency requirements for wireless communication systems. But in scattering environment, the system performances are severely degraded by the effects of multipath fading and inter-symbol interference. In wireless communication systems, antenna diversity is an important technique to combat multipath fading in order to improve the system performance and increase the channel capacity. In this paper, the performance of different diversity combining techniques-SC （selection combining）, EGC （equal gain combining） and MRC （maximal ratio combining） has been analyzed and compared in terms of SNR （signal to noise ratio） and BER （bit error rate） probability. The simulation results show that the maximal ratio combining technique provides maximum performance improvement relative to all other combining schemes by maximizing the SNR of SIMO-OFDM system at the combiner output. The analytic expressions of error probability and effective bit energy to noise ratio correlated with BPSK （binary phase shift keying） modulation have been derived and formulated for N-branch SC, EGC and MRC schemes. The BER characteristics for all three combining techniques are simulated in MATLAB （matrix laboratory） tool box for varying bit energy to noise ratio. Our results also derives that SNR can be improved if the number of receiving antenna is increased, which in turn reduces BER over a Rayleigh fading channel.

关键词： OFDM antenna diversity SC EGC MRC Rayleigh fading BER SNR IBI ICI FFT BPSK modulation.

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