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In vitro evaluation of antimicrobial and antioxidant activity of Dragon’s blood tree(Dracaena cinnabari Balf.f.)of Socotra Island(Yemen)

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Journal of Coastal Life Medicine 2013年第2期1卷 123-129页

作者： Abu-Taleb Ahmed Yehia Fahad Ahmed Mohsen Alzowahi tukaram angadrao kadam Rafik Usman Shaikh Department of Microbiology School of Life SciencesS.R.T.M.UniversityNanded-431606(MS)India Department of Microbiology Taiz UniversityYemen

Objective:To evaluate the performance of preliminary phytochemical,antioxidant and antimicrobial activities of Dracaena cinnabari Balf.f.(Agavaceae)(D.cinnabari)resin,collected from Socotra Island(Yemen).Methods:The powdered stem resin is extracted with different solvents by using Soxhlet apparatus and evaluated for antioxidant and antimicrobial activity against three Gram positive bacteria(Bacillus cereus NCIM 2106,Staphylococcus aureus NCIM 2127,and Micrococcus luteus NCIM 2169),seven Gram negative bacteria(Escherichia coli NCIM 2924,Pseudomonas aeruginosa NCIM 2863,Salmonella typhimurium NCIM 2501,Klebsiella aerogenes NCIM 2281,Proteus morganii NCIM 2860,Escherichia coli dh5α,Escherichia coli CSH)and three fungi(Candida albicans MTCC 227,Aspergillus flavus MTCC 873,Aspergillus niger MTCC 1781).Results:The antimicrobial activity data revealed that,the selected microorganisms possess varied sensitivity response to chloroform extract followed by methanol and benzene extract of D.cinnabari stem resin.The samples also possess a potent DPPH,OH and SO radical scavenging activity.In addition,the preliminary phytochemical analysis demonstrated the presence of alkaloids,flavonoids,terpenoids and other organic compounds which could be reason for antimicrobial activities of D.cinnabari resin.Conclusions:The present study is strengthened for the discovery and development of new antioxidant and antimicrobial drugs from D.cinnabari resin which encourage us to continue with pure fractions.

关键词： Dracaena cinnabari Socotra Yemen Resin Antimicrobial Antioxidant Phytochemical screening

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Optimization of Culture Conditions for Production of Bioactive Metabolites by <i>Streptomyces</i>spp. Isolated from Soil

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Advances in Microbiology 2015年第6期5卷 441-451页

作者： Sunita Bundale Deovrat Begde Nandita Nashikkar tukaram kadam Avinash Upadhyay Hislop School of Biotechnology Hislop College Nagpur Maharashtra India Department of Microbiology School of Life Sciences SRTMU Nanded Maharashtra India

The current work was carried out under a screening program targeted at isolation of bioactive Streptomyces species from soil samples. A total of 54 Streptomyces species were isolated from soil samples, out of which 4 isolates were found to be promising. These isolates were identified as Streptomyces spectabilis, Streptomyces purpurascens, Streptomyces coeruleorubidus and Streptomyces lavendofoliae and their sequences have been deposited in the GenBank. The influence of culture conditions including, incubation time, incubation temperature, initial pH and different carbon and nitrogen sources on growth and bioactive compound formation was investigated. Isolate R1, identified as Streptomyces spectabilis, showed maximum bioactive metabolite production with cellobiose and peptone as the carbon and nitrogen sources, on the 5th day at pH 5 at 30℃. The optimum conditions for production by isolate R3, identified as Streptomyces purpurascens, were observed to be starch and casein as the carbon and nitrogen sources, pH 7, temperature 30℃ and an incubation period of eight days. For isolate R5, identified as Streptomyces coeruleorubidus, maximal production resulted on the sixth day at pH 6 and temperature of 35℃ with mannitol and JBM. Isolate Y8, identified as Streptomyces lavendofoliae, was found to produce high levels of bioactive metabolites in the medium supplemented with starch and peptone on the 10th day at pH 7 and at an incubation temperature of 30℃. The four strains tested here behaved differently, each one requiring specific conditions for maximum growth as well as bioactive metabolite production.

关键词： Streptomyces Bioactive Metabolites Culture Conditions

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