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Combination of mesenchymal stem cells and three-dimensional collagen scaffold preserves ventricular remodeling in rat myocardial infarction model

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World Journal of Stem Cells 2022年第8期14卷 633-657页

作者： Rida-e-Maria Qazi Irfan Khan Kanwal Haneef tuba shakil malick Nadia Naeem Waqas Ahmad Asmat Salim Sadia Mohsin Stem Cell Laboratory Dr.Panjwani Center for Molecular Medicine and Drug ResearchICCBSUniversity of KarachiKarachi 75270SindhPakistan Dr.Zafar H.Zaidi Center for Proteomics University of KarachiKarachi 75270SindhPakistan Dow Research Institute of Biotechnology and Biomedical Sciences(DRIBBS) Dow University of Health and SciencesOjha CampusKarachi 74200SindhPakistan Cardiovascular Research Center Lewis Katz School of MedicineTemple UniversityPhiladelphiaPA 19140United States

BACKGROUND Cardiovascular diseases are the major cause of mortality *** of the damaged myocardium remains a challenge due to mechanical constraints and limited healing ability of the adult heart *** tissue engineering using biomaterial scaffolds combined with stem cells and bioactive molecules could be a highly promising approach for cardiac *** of biomaterials can provide suitable microenvironment to the cells and can solve cell engraftment problems associated with cell transplantation *** stem cells(MSCs)are potential candidates in cardiac tissue engineering because of their multilineage differentiation potential and ease of *** of DNA methyl transferase inhibitor,such as zebularine,in combination with three-dimensional(3D)scaffold can promote efficient MSC differentiation into cardiac lineage,as epigenetic modifications play a fundamental role in determining cell fate and lineage specific gene *** To investigate the role of collagen scaffold and zebularine in the differentiation of rat bone marrow(BM)-MSCs and their subsequent in vivo *** MSCs were isolated from rat BM and characterized morphologically,immunophenotypically and by multilineage differentiation *** were seeded in collagen scaffold and treated with 3μmol/L zebularine in three different *** analysis was done and cardiac differentiation was analyzed at the gene and protein *** and untreated MSC-seeded scaffolds were transplanted in the rat myocardial infarction(MI)model and cardiac function was assessed by *** tracking was performed by DiI dye labeling,while regeneration and neovascularization were evaluated by histological and immunohistochemical analysis,*** MSCs were successfully isolated and seeded in collagen *** analysis revealed that zebularine was not cytotoxic in any of the treatment *** differentiation analysis

关键词： Mesenchymal stem cells Myocardial infarction Cardiac tissue engineering Demethylating agent Collagen scaffold Zebularine

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Effect of glycyrrhizic acid and 18β-glycyrrhetinic acid on the differentiation of human umbilical cord-mesenchymal stem cells into hepatocytes

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World Journal of Stem Cells 2021年第10期13卷 1580-1594页

作者： Abiha Fatima tuba shakil malick Irfan Khan Aisha Ishaque Asmat Salim Stem Cell Laboratory Dr.Panjwani Center for Molecular Medicine and Drug ResearchICCBSUniversity of KarachiKarachi 75270SindhPakistan

BACKGROUND End-stage liver disease is a global health complication with high prevalence and limited treatment ***-based therapies using mesenchymal stem cells(MSCs)emerged as an alternative approach to support hepatic *** vitro preconditioning strategies have been employed to strengthen the regenerative and differentiation potential of MSCs towards hepatic *** compounds of the triterpene class;glycyrrhizic acid(GA)and 18β-glycyrrhetinic acid(GT)possess diverse therapeutic properties including hepatoprotection and anti-fibrosis *** are capable of modulating several signaling pathways that are crucial in hepatic *** with hepato-protective triterpenes may stimulate MSC fate transition towards *** To explore the effect of GA and GT on hepatic differentiation of human umbilical cord-MSCs(hUC-MSCs).METHODS hUC-MSCs were isolated and characterized phenotypically by flow cytometry and immunocytochemistry for the expression of MSC-associated surface *** cells were treated with GA,GT,and their combination for 24 h and then analyzed at three time points;day 7,14,and ***-PCR was performed for the expression of hepatic *** of hepatic proteins was analyzed by immunocytochemistry at day *** acid Schiff staining was performed to determine the functional ability of treated *** The fusiform-shaped morphology of MSCs in the treatment groups in comparison with the untreated control,eventually progressed towards the polygonal morphology of hepatocytes with the passage of *** temporal transcriptional profile of preconditioned MSCs displayed significant expression of hepatic genes with increasing time of *** cells showed positive expression of hepatocyte-specific *** results were further corroborated by positive periodic acid Schiff staining,indicating the presence of glycogen in their ***,bi-nuclea

关键词： Glycyrrhizic acid 18β-glycyrrhetinic acid Hepatocyte differentiation Human umbilical cord-MSCs Mesenchymal stem cells

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