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Discrimination of False Negative Results in RT-PCR Detection of SARS-CoV-2 RNAs in Clinical Specimens by Using an Internal Reference

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Virologica Sinica 2020年第6期35卷 758-767页

作者： Yafei Zhang Changtai Wang Mingfeng Han Jun Ye Yong Gao Zhongping liu Tengfei He tuantuan li Mengyuan Xu Luping Zhou Guizhou Zou Mengji Lu Zhenhua Zhang Department of Infectious Diseases The Second Hospital of Anhui Medical UniversityHefei 230601China Institute of Clinical Virology The Second Hospital of Anhui Medical UniversityHefei 230601China Department of Internal Medicine The Second Hospital of FuyangFuyang 236015China Institute for Virology University Hospital of EssenUniversity of Duisburg-Essen45147 EssenGermany

Reverse transcription-polymerase chain reaction(RT-PCR)is an essential method for specific diagnosis of SARS-CoV-2 ***,false negative test results are often *** this study,we attempted to determine the principal causes leading to false negative results of RT-PCR detection of SARS-CoV-2 RNAs in respiratory tract *** sputum and throat swab specimens from 161 confirmed COVID-19 patients were tested with a commercialfluorescent RT-PCR kit targeting the ORF1 ab and N regions of SARS-CoV-2 *** RNA level of a cellular housekeeping gene ribonuclease P/MRP subunit p30(RPP30)in these specimens was also assessed by *** for a total of 1052 samples were retrospectively re-analyzed and a strong association between positive results in SARS-CoV-2 RNA tests and high level of RPP30 RNA in respiratory tract specimens was *** using the ROC-AUC analysis,we identified Ct cutoff values for RPP30 RT-PCR which predicted false negative results for SARS-CoV-2 RT-PCR with high sensitivity(95.03%–95.26%)and specificity(83.72%–98.55%)for respective combination of specimen type and ampli-fication *** these Ct cutoff values,false negative results could be reliably ***,the presence of cellular materials,likely infected host cells,are essential for correct SARS-CoV-2 RNA detection by RT-PCR in patient ***30 could serve as an indicator for cellular content,or a surrogate indicator for specimen *** addition,our results demonstrated that false negativity accounted for a vast majority of contradicting results in SARS-CoV-2 RNA test by RT-PCR.

关键词： COVID-19 SARS-CoV-2 RT-PCR False negative results Internal reference

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CORRECTION:Correction to:Discrimination of False Negative Results in RT-PCR Detection of SARS-CoV-2 RNAs in Clinical Specimens by Using an Internal Reference

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Virologica Sinica 2020年第6期35卷 885-886页

作者： Yafei Zhang Changtai Wang Mingfeng Han Jun Ye Yong Gao Zhongping liu Tengfei He tuantuan li Mengyuan Xu Luping Zhou Guizhou Zou Mengji Lu Zhenhua Zhang Department of Infectious Diseases The Second Hospital of Anhui Medical UniversityHefei 230601China Institute of Clinical Virology The Second Hospital of Anhui Medical UniversityHefei 230601China Department of Intemal Medicine The Second Hospital of FuyangFuyang 236015China Institute for Virology University Hospital of EssenUniversity of Duisburg-Essen45147 EssenGermany

In the original version of Fig.41 and 4J,the cutoff lines were accidently shifted during figure *** 41 and 4J are re-drawn and provided below.

关键词： Internal accident layout

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数字经济时代职业教育数字化能力培养研究

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天津职业大学学报 2022年第4期31卷 58-65页

作者：李团团四川财经职业学院会计学院四川成都610101

数字经济时代,数字化人才成为企业数字化转型的核心驱动力,社会对人才能力需求有了新的标准,因此,要满足社会需求,人才培养模式就要发生改变。针对我国职业教育数字化能力培养现状进行调研,以财务会计类专业为研究对象,以岗位职业能力... 详细信息

数字经济时代,数字化人才成为企业数字化转型的核心驱动力,社会对人才能力需求有了新的标准,因此,要满足社会需求,人才培养模式就要发生改变。针对我国职业教育数字化能力培养现状进行调研,以财务会计类专业为研究对象,以岗位职业能力需求为导向,分析职业教育数字化能力人才培养模式,探索数字经济时代财务会计类专业数字化能力培养新途径。

关键词：数字经济数字化能力会计转型

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HBV DNA定量与血清学标志物及CEA、HA联合检测HBV感染的临床价值

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分子诊断与治疗杂志 2023年第2期15卷 222-225,230页

作者：郑金萍高勇李团团阜南县人民医院检验科安徽阜阳236300 阜阳市第二人民医院检验科安徽阜阳236015

目的探究乙型肝炎病毒(HBV DNA)定量与血清学标志物及血清癌胚抗原(CEA)、血清透明质酸酶(HA)联合检测HBV感染的临床价值。方法选取2019年1月至2021年11月阜南县人民医院收治的慢性乙型病毒性肝炎患者92例作为研究对象,所有患者均行... 详细信息

目的探究乙型肝炎病毒(HBV DNA)定量与血清学标志物及血清癌胚抗原(CEA)、血清透明质酸酶(HA)联合检测HBV感染的临床价值。方法选取2019年1月至2021年11月阜南县人民医院收治的慢性乙型病毒性肝炎患者92例作为研究对象,所有患者均行临床乙肝血清学标志物[包含乙肝表面抗原(HBsAg)、乙肝表面抗体(抗-HBs)、乙肝e抗原(HBeAg)、乙肝e抗体(抗-HBe)、乙肝病毒的核心抗体(抗-HBc)]、CEA、HA以及HBV DNA检测,观察并记录所有患者HBV DNA、CEA、HA的检测结果,比较血清标志物对HBV DNA的检测阳性率,分析HBV DNA定量与血清学标志物及CEA、HA联合检测HBV感染的临床价值。结果 92例患者中,HBV DNA阳性患者54例(58.70%),阴性38例(41.30%)。大三阳组患者HBV DNA阳性31/33例(93.94%),小三阳组患者HBV DNA阳性15/31例(48.39%),其他类型组患者HBV DNA阳性3/28例(10.71%)。92例患者CEA水平4.20(3.20,5.30)ng/mL;HA水平分别为112.00(98.00,126.00)ng/mL。HBV DNA阳性组患者CEA、HA水平显著高于HBV DNA阴性组,差异有统计学意义(P<0.05)。HBV DNA阳性组中,HBsAg阳性检出率显著高于HBeAg,差异有统计学意义(P<0.05)。Spearman相关结果显示,患者的HBV DNA定量与CEA、HBsAg、HA指标成正相关关系(P<0.05)。ROC分析显示:HBV DNA定量与血清学标志物及血清CEA、HA联合检测乙肝病毒感染的AUC=0.985(P<0.05),诊断敏感度为97.76%。结论 HBV DNA定量与血清学标志物CEA、HA联合检测在HBV感染诊断中具有较高的应用价值,可起到互补作用,为临床检测提供了新路径。

关键词： HBV DNA 慢性乙型病毒性肝炎 HA CEA

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NB-UVB联合卡泊三醇倍他米松治疗老年中重度银屑病的临床观察

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中国实用医刊 2022年第24期49卷 61-64页

作者：杜秋燕许慧芳李团团平顶山市第二人民医院皮肤科平顶山467000

目的探讨老年中重度银屑病患者应用窄谱中波紫外线(NB-UVB)联合卡泊三醇倍他米松的治疗效果。方法抽取2021年3月至2022年3月平顶山市第二人民医院收治的中重度银屑病老年患者84例,以双色球法分为对照组与观察组,每组42例。对照组予以NB-... 详细信息

目的探讨老年中重度银屑病患者应用窄谱中波紫外线(NB-UVB)联合卡泊三醇倍他米松的治疗效果。方法抽取2021年3月至2022年3月平顶山市第二人民医院收治的中重度银屑病老年患者84例,以双色球法分为对照组与观察组,每组42例。对照组予以NB-UVB治疗,观察组在对照组治疗基础上联合卡泊三醇倍他米松治疗,比较两组总有效率、炎性因子水平、银屑病皮损面积和严重程度指数(PASI)评分、负面情绪效果。结果观察组总有效率(90.48%,38/42)高于对照组(71.43%,30/42),P0.05);治疗后,观察组炎性因子水平、PASI评分、负面情绪评分均低于对照组(P均<0.05)。结论老年中重度银屑病患者予以NB-UVB联合卡泊三醇倍他米松方案治疗,可提高临床治疗效果,改善血清炎性因子水平与PASI评分,还可有效缓解负面情绪。

关键词：银屑病窄谱中波紫外线卡泊三醇倍他米松

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Climate Variability over the Maritime Continent and Its Role in Global Climate Variation: A Review

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Journal of Meteorological Research 2019年第6期33卷 993-1015页

作者： Song YANG tuantuan ZHANG Zhenning li Shaorou DONG School of Atmospheric Sciences Sun Yat-sen UniversityGuangzhou 510275 Guangdong Province Key Laboratory for Climate Change and Natural Disaster Studies Sun Yat-sen UniversityGuangzhou 510275 Southern Laboratory of Ocean Science and Engineering(Zhuhai Guangdong)Zhuhai 519082 Institute of Environment Energy and SustainabilityThe Chinese University of Hong KongHong Kong 999077 Climate Center of Guangdong Province Guangzhou 510080

The Maritime Continent(MC)consists of multiple islands with varying sizes and topography,and surrounding *** is characterized by rainfall(convection)variability on multiple spatial and temporal *** largescale atmospheric,oceanic,and coupled climate systems,such as the El Ni?o–Southern Oscillation(ENSO),Indian Ocean Dipole(IOD),Madden–Julian Oscillation(MJO),and cold surge,exert significant influences on the spatiotemporal complexity of the MC climate and climate *** a major tropical heat source located within the warmest oceanic area(the western Pacific warm pool),the MC has been identified as a region of great importance for climate variation on the global ***,prediction of climate variability over the MC and its surrounding areas and the relationships to large-scale atmospheric circulation patterns are big challenges,even for state-of-the-art climate *** this paper,we provide a thorough review on current understanding of the spatiotemporal complexity and prediction of climate variability over this important region,and its influence on global climate variation.

关键词： Maritime Continent(MC) climate variation and prediction spatiotemporal complexity global climate review

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不同糖代谢状态下TyG指数对慢性肾脏病患者冠心病发生风险的评估价值

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检验医学与临床 2024年第21期21卷 3161-3167页

作者：丁云云汪小五武东王伟刘艳李梅李团团安徽省阜阳市第二人民医院检验科安徽阜阳236000 安徽省阜阳市人民医院药剂科安徽阜阳236000 安徽省阜阳市人民医院检验科安徽阜阳236000

目的探讨不同糖代谢状态下甘油三酯葡萄糖乘积(TyG)指数对慢性肾脏病(CKD)患者冠心病(CHD)发生风险的评估价值。方法选取2021年6月至2023年12月在阜阳市第二人民医院住院的CKD患者307例为研究对象。根据是否合并CHD分为CKD合并CHD组(CHD... 详细信息

目的探讨不同糖代谢状态下甘油三酯葡萄糖乘积(TyG)指数对慢性肾脏病(CKD)患者冠心病(CHD)发生风险的评估价值。方法选取2021年6月至2023年12月在阜阳市第二人民医院住院的CKD患者307例为研究对象。根据是否合并CHD分为CKD合并CHD组(CHD组)130例,CKD不合并CHD组(非CHD组)177例。根据是否合并糖尿病进行分层:糖尿病患者中CHD组59例,非CHD组65例;无糖尿病患者中CHD组71例,非CHD组112例。绘制受试者工作特征(ROC)曲线分析不同糖代谢状态下TyG指数、空腹血糖(GLU)、空腹甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、纤维蛋白原(FIB)对CHD发生风险的评估价值,采用多因素二元Logistic回归分析CKD患者发生CHD的危险因素。结果在糖尿病患者中,CHD组年龄、TyG指数以及GLU、TG水平高于非CHD组(P<0.05),HDL-C水平低于非CHD组(P<0.05);TyG指数、GLU、TG、HDL-C预测CKD患者发生CHD的曲线下面积(AUC)分别为0.820(95%CI:0.741~0.883)、0.676(95%CI:0.586~0.757)、0.717(95%CI:0.630~0.795)、0.666(95%CI:0.576~0.749);多因素二元Logistic回归分析发现,年龄增大、TyG指数升高是糖尿病CKD患者发生CHD的独立危险因素(OR=1.092、6.200,P<0.05)。在无糖尿病患者中,CHD组年龄、患有高血压的比例、TyG指数,以及GLU、TG、FIB水平高于非CHD组(P<0.05),HDL-C水平低于非CHD组(P<0.05),TyG指数、GLU、TG、HDL-C、FIB预测CKD患者发生CHD的AUC为0.898(95%CI:0.845~0.938)、0.653(95%CI:0.579~0.721)、0.823(95%CI:0.760~0.875)、0.618(95%CI:0.544~0.689)、0.688(95%CI:0.615~0.754);多因素二元Logistic回归分析发现,年龄增大、患有高血压、TyG指数升高、FIB水平升高是无糖尿病CKD患者发生CHD的独立危险因素(OR=1.120、19.674、48.002、1.999,P<0.05)。结论TyG指数、GLU、TG、HDL-C、FIB与CKD患者发生CHD均有一定的关系,且TyG指数的风险评估价值最高。

关键词：慢性肾脏疾病冠心病甘油三酯葡萄糖乘积指数二元Logistic回归受试者工作特征曲线

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Sub-seasonal Prediction of the South China Sea Summer Monsoon Onset in the NCEP Climate Forecast System Version 2

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Advances in Atmospheric Sciences 2022年第11期39卷 1969-1981页

作者： Weiwei WANG Song YANG tuantuan ZHANG Qingquan li Wei WEI School of Atmospheric Sciences and Guangdong Province Key Laboratory for Climate Change and Natural Disaster Studies Sun Yat-sen UniversityZhuhai 519082China Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai) Zhuhai 519082China National Climate Center China Meteorological AdministrationBeijing 100081China

This study depicts the sub-seasonal prediction of the South China Sea summer monsoon onset(SCSSMO)and investigates the associated oceanic and atmospheric processes,utilizing the hindcasts of the National Centers for Environmental Prediction(NCEP)Climate Forecast System version 2(CFSv2).Typically,the SCSSMO is accompanied by an eastward retreat of the western North Pacific subtropical high(WNPSH),development of the cross-equatorial flow,and an increase in the east-west sea surface temperature(SST)*** features are favorable for the onset of westerlies and strengthening of convection and precipitation over the South China Sea(SCS).A more vigorous SCSSMO process shows a higher predictability,and vice *** NCEP CFSv2 can successfully predict the onset date and evolution of the monsoon about 4 pentads(20 days)in advance(within 1–2 pentads)for more forceful(less vigorous)SCSSMO *** the other hand,the climatological SCSSMO that occurs around the 27th pentad can be accurately predicted in one pentad,and the predicted SCSSMO occurs 1–2 pentads earlier than the observed with a weaker intensity at longer *** SST biases appear over the western equatorial Pacific preceding the *** biases induce a weaker-thanobserved WNPSH as a Gill-type response,leading to weakened low-level easterlies over the SCS and hence an earlier and less vigorous *** addition,after the SCSSMO,remarkable warm biases over the eastern Indian Ocean and the SCS and cold biases over the WNP induce weaker-than-observed westerlies over the SCS,thus also contributing to the less vigorous SCSSMO.

关键词： South China Sea summer monsoon onset sub-seasonal prediction the NCEP CFSv2

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Mapping of fruit apex shape related QTLs across multi-genetic backgrounds in cucumber(Cucumis sativus L.)

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Horticultural Plant Journal 2022年第3期8卷 328-340页

作者： Pinyu Zhu Yongjiao Meng Kaijing Zhang Xueyan Wang Kun liang tuantuan Wang Jian Xu Xiaodong Qin Zhe Wu Chunyan Cheng Xiaqing Yu Chuntao Qian Qunfeng Lou Jinfeng Chen Ji li State Key Laboratory of Crop Genetics and Germplasm Enhancement College of HorticultureNanjing Agricultural UniversityNanjing 210095China College of Engineering/Jiangsu Province Engineering Lab for Modern Facility Agriculture Technology&Equipment Nanjing Agricultural UniversityNanjing 210031China College of Agriculture Anhui Science and Technology UniversityFengyang 233100China

The shape of fruit apex is critical to appearance quality in cucumber(Cucumis sativus L.),of which the genetic basis was poorly understood,and the use of marker-assisted breeding for fruit apex improvement is not available *** this study,the variation of fruit apex in different cucumber ecotypes was evaluated by fruit apex angle(variation coefficient from 7.1%to 15.7%)and fruit apex index(variation coefficient from 8.8%to 22.6%).Fruit apex associated QTLs were mapped by using 145 F_(2:3) families and 155 F_(2:6) population that were derived from the cross of different ecotype *** of the mapping populations were conducted in four experiments in 2 *** majoreffect QTLs,Bfal4.1,Bfai4.1,Bfad6.1 and Bfai6.1 were consistently and reliably detected across two environments which could explain 11.6%-33.6%phenotypic variations(R^(2))in the F_(2:3) *** major-effect QTLs,Ofai4.1(R^(2)=13.4%-15.5%),Ofal4.1(R^(2)=10.7%-12.8%),and Ofad6.1(R^(2)=11.6%-12.4%)were stably detected in the F_(2:6) population in two ***4.1,Bfal4.1,Ofai4.1 and Ofal4.1 were integrated to be consensus QTL fa4.1,within which 11 candidate genes were ***6.1 and Bfad6.1 were integrated to be consensus QTL *** interaction analysis showed that Bfai6.1 has epistatic effect with *** study revealed two reliable major-effect fruit apex related QTLs across multi-genetic backgrounds and environments in *** possible candidate genes regulating the shape of fruit apex,and the relationship between cell division and fruit apex morphogenesis were discussed.

关键词： Cucumber Fruit apex Variation QTL Candidate gene

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Hypertension predisposes individuals to heightened immune responses and autoimmunity

Hypertension predisposes individuals to heightened immune re...

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第十三届全国免疫学学术大会

作者： Xiao Shen tuantuan Zhao Yu li Peng Shi Zhejiang University School of Medicine

Background: Recent researches unveiled that antigen presenting cells(APCs) and T cells are both involved in the progression of hypertension, implying that hypertension has an autoimmune ***, how an elevated blood pressure(BP) initiates inflammation is unknown, and the effects of high BP on adaptive immune responses are ***: To understand the effects of hypertension on the immune system and to investigate the mechanism by which an increase in BP induces increased immune ***: Male C57BL/6 mice were treated with angiotensin Ⅱ or L-NAME to induce ***-mOVA mice-associated type-Ⅰ diabetes model and concanavalin A-induced autoimmune hepatitis model were used to assess the susceptibility to develop autoimmune *** levels were tested by a luciferase-based bioluminescence *** effects of ATP and P2X receptor were studied by using apyrase and the receptor specific ***, clinical data were used to demonstrate an association between ATP and BP in ***: We now report that hypertensive mice have increased T cell responses to antigenic challenge and they are prone to develop CD8 T cell-mediated Type I diabetes and CD4 T cell-mediated autoimmune *** is due to the upregulation of CD86 expression by APCs but not due to T cell over-sensitivity.A fundamental cause for this is plasma ATP levels which rise soon after the onset of hypertension and upregulate CD86 expression of *** ATP or blocking the P2X receptor eliminated the predisposition of hypertensive mice to autoimmune ***, pharmacological or genetic ablation of P2X receptor activity suppressed the progression of *** human, untreated hypertensive patients have substantially elevated plasma ATP levels compared to treated hypertensive patients or normotensive ***: Hypertension affects the immune responses;elevated ATP is a trigger of inflammation and is pathologic in hypertensive dise

关键词： Autoimmunity Hypertension CD86 ATP

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