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Structured-illumination reflectance imaging for the evaluation of microorganism contamination in pork: Effects of spectral and imaging features on its prediction performance

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Food Science and Human Wellness 2024年

作者： Binjing Zhou Xiaohua Liu Yan Ge kang tu Jing Peng Juan Francisco García-Martín Jie Wu Weijie Lan Leiqing Pan The Academy of Science Nanjing Agricultural University College of Engineering Nanjing Agricultural University College of Food Science and Technology Nanjing Agricultural University School of Food and Biological Engineering Bengbu University Departamento de Ingeniería Química Facultad de Química Universidad de Sevilla

Structured-illumination reflectance imaging provides a new means for food quality detection. This original work investigated the capability of Structured-illumination reflectance imaging technique coupled with multivariate chemometrics to evaluate the microbial contamination in pork inoculated with Pseudomonas fluorescens and Brochothrix thermosphacta during storage at different temperatures. The prediction performances based on different spectrum and the textural features of direct component and amplitude component images demodulated from the SIRI pattern images， as well as their data fusion were comprehensively compared. Based on the full wavelength spectrum (420 – 700) nm of amplitude component images， the orthogonal signal correction coupled with support vector machine regression provided the best predictions of the number of Pseudomonas fluorescens and Brochothrix thermosphacta in pork， with the Rp2values of 0.870 and 0.906， respectively. Besides， the prediction models based on the amplitude component or direct component image textural features and the data fusion models using spectrum and textural features from direct component and amplitude component images cannot significantly improve their prediction accuracy. Consequently， structured-illumination reflectance imaging can be further considered as a potential technique for the rapid evaluation of microbial contaminations in pork meat.

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基于深度学习的交通事件检测系统设计

无线互联科技 2024年第3期21卷 63-65页

作者：屠康马倩芳中兴飞流信息科技有限公司江苏南京210000 同方威视科技江苏有限公司江苏南京210000

建立完善的交通事件检测系统,已经成为我国智能交通系统的重要组成部分。文章从数据和算法2个方面深入分析了交通事件检测场景特征,设计了一种基于深度学习的交通事件检测系统。文章提出了一种混合架构的联合学习网络,通过综合利用ViT和... 详细信息

建立完善的交通事件检测系统,已经成为我国智能交通系统的重要组成部分。文章从数据和算法2个方面深入分析了交通事件检测场景特征,设计了一种基于深度学习的交通事件检测系统。文章提出了一种混合架构的联合学习网络,通过综合利用ViT和Swin Transformer的优势解决了图像多标签分类问题的挑战;设计了一系列数据增强方法,应对数据不平衡性对深度学习模型的影响,并有效缓解了模型过拟合问题。实验结果表明,该系统在交通事件检测中具有更好的准确性和泛化能力,已应用到多个实际项目并取得了良好的应用效果。

关键词：交通事件检测深度学习对抗生成网络 Transformer

温度与盐度对福建牡蛎与长牡蛎、熊本牡蛎、香港巨牡蛎种间杂交子代孵化及其早期生长成活的影响

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海洋科学 2024年第4期48卷 53-62页

作者：涂康韩自强肖懿哲谢双如黄琼叶莆田市水产科学研究所福建莆田351100 集美大学水产学院福建厦门361100

本研究以野生福建牡蛎(A)为亲本一方,分别与野生长牡蛎(G)、熊本牡蛎(S)、香港巨牡蛎(H)进行杂交,除A♀×S♂、A♀×H♂两组杂交不育外,共得到AG(A♀×G♂)、GA(G♀×A♂)、SA(S♀×A♂)、HA(H♀×A♂)、AA(A♀×A♂)5个不同组合,并研... 详细信息

本研究以野生福建牡蛎(A)为亲本一方,分别与野生长牡蛎(G)、熊本牡蛎(S)、香港巨牡蛎(H)进行杂交,除A♀×S♂、A♀×H♂两组杂交不育外,共得到AG(A♀×G♂)、GA(G♀×A♂)、SA(S♀×A♂)、HA(H♀×A♂)、AA(A♀×A♂)5个不同组合,并研究不同盐度与温度对其幼虫孵化率、早期生长及成活率的影响。结果表明,盐度25时AG、GA、SA、HA、AA五组孵化率分别达到最大值78.33%、75.00%、63.33%、55.00%、71.67%;温度低于25℃时,GA孵化率高于其他组,温度高于25℃时AG孵化率最大,其中25℃时达到最大孵化率83.33%;不同盐度条件下D型幼虫经过7 d培育,HA在盐度20时壳高达到最大值97.8μm,成活率46.67%,在盐度为35时第5天全部死亡。AG、GA、SA、AA四组在盐度为25时壳高有最大值,分别为124.7μm、121.2μm、93.7μm,111.8μm;不同温度条件下D型幼虫经过7 d培育,在温度为25℃时各组壳高和成活率均有最大值,大小排列依次为AG>GA>AA>HA>SA,各组成活率随温度升高呈现出先增大后减小的趋势,AG在25℃时成活率在所有组中最高,为88.89%;孵化后在适宜温度盐度条件下连续培养16 d,不同组之间壳高表现为:AG>GA>AA>HA>SA,AG壳高、壳高SGR及成活率始终大于其他组,而SA、HA两组壳高、壳高SGR及成活率始终小于自交组AA。研究表明,相较于福建牡蛎与熊本牡蛎、香港巨牡蛎的杂交,以及福建牡蛎的自交,福建牡蛎与长牡蛎的杂交子代表现出明显的生长及存活优势,生长潜力较好,且AG优于GA;AG组在盐度25、水温25℃条件下生长速率最大,成活率最高。研究结果可为福建牡蛎育苗行业良种选育提供参考依据。

关键词：福建牡蛎长牡蛎熊本牡蛎香港巨牡蛎种间杂交

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Evaluation of Reference Genes for Quantitative Real-Time PCR Analysis in Manila Clam Ruditapes philippinarum Under Hypoxic Stress

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Journal of Ocean University of China 2023年第4期22卷 1059-1067页

作者： JING Hao ZHOU Liqing GONG Miao tu kang LIU Zhihong WU Biao SUN Xiujun College of Fisheries and Life Science Shanghai Ocean UniversityShanghai 201306China State Key Laboratory of Mariculture Biobreeding and Sustainable Goods Yellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266071China Laboratory for Marine Fisheries Science and Food Production Processes Laoshan LaboratoryQingdao 266237China School of Marine Science and Fisheries Jiangsu Ocean UniversityLianyungang 222005China Putian Institute of Aquaculture Science of Fujian Province Putian 351100China

Quantitative real-time PCR(qRT-PCR)has been widely used for gene expression analysis,and selection of reference genes is a key point to obtain accurate *** find out optimal reference genes for qRT-PCR in Manila clam Ruditapes philippinarum in response to hypoxia,different tissues were used and compared to evaluate the stability of candidate reference genes under low oxygen stress(DO 0.5mgL^(−1) and DO 2.0mgL^(−1))and normal condition(DO 7.5mgL^(−1)).Seven candidate reference genes were selected to evaluate the stability of their expression *** reference genes were evaluated by Delta Ct,BestKeeper,NormFinder and geNorm,and then screened by RefFinder *** hypoxic stress of 0.5mgL^(−1),the most suitable reference gene for gill and hepatopancreas was RPL31,and the optimal reference genes for axe foot and adductor muscle were tuB and HIS,*** hypoxic stress of 2.0mgL^(−1),the most stable reference gene for gill and hepatopancreas was RPL31,and the optimal reference genes for axe foot and adductor muscle were RPS23 and EF1A,*** the normal condition,HIS and EF1A were identified as the optimal internal reference genes in gill and hepatopancreas respectively,and GFRP2 was the best internal reference gene for axe foot and adductor *** present findings will provide important basis for the selection of reference genes for qRT-PCR analysis of gene expression level in bivalves under hypoxic stress,which might be helpful for the analysis of other molluscs too.

关键词： clam reference gene hypoxia quantitative real-time PCR

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基于可见光-近红外高光谱信息与数据融合的木质化鸡胸肉的判别模型构建

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食品工业科技 2024年第7期45卷 286-293页

作者：张娜李震兰维杰屠康武杰王兆山赵干潘磊庆南京农业大学食品科技学院江苏南京210095 蚌埠学院食品与生物工程学院安徽蚌埠233030 江苏益客食品集团股份有限公司江苏宿迁223800

木质化鸡胸肉(wooden breast,WB)制约肉鸡行业发展,传统触诊检测方法耗时且效率低,为提升高光谱成像(hyperspectral imaging,HSI)技术检测鸡胸肉木质化程度的效果,本论文以白羽鸡鸡胸肉为研究对象,将其划分4个木质化等级,采集其在400~1... 详细信息

木质化鸡胸肉(wooden breast,WB)制约肉鸡行业发展,传统触诊检测方法耗时且效率低,为提升高光谱成像(hyperspectral imaging,HSI)技术检测鸡胸肉木质化程度的效果,本论文以白羽鸡鸡胸肉为研究对象,将其划分4个木质化等级,采集其在400~1000和1000~2000 nm内的HSI信息,通过不同光谱预处理算法和特征波段筛选方法,建立基于全波段、特征波段和HSI数据融合的偏最小二乘判别分析(Partial least squares-discriminant analysis,PLS-DA)模型和支持向量机(Support vector machine,SVM)模型。结果显示,SVM模型比PLSDA模型更适于判别鸡胸肉木质化程度,基于1000~2000 nm内全波段和特征波段的最佳模型预测集总体正确率均高于400~1000 nm内的模型,基于两波段HSI数据融合的木质化判别模型优于基于单一波段(包括全波段和特征波段)的模型,最佳模型预测集总体正确率为96.7%,能较好地区分出4个木质化等级,且对4个等级的判别准确率均可达90%以上。研究结果为HSI实现木质化鸡胸肉的准确无损检测提供技术支持。

关键词：木质化鸡胸肉可见-近红外高光谱短波红外高光谱光谱数据融合判别模型

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基于多成熟度光谱信息融合的阿森泰克苹果品质预测模型研究

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食品工业科技 2024年第7期45卷 294-305页

作者：吴莎莎王振杰江梦薇兰维杰屠康李晏袁栋栋潘磊庆南京农业大学食品科技学院江苏南京210095 江苏福瑞斯农业有限公司江苏宿迁223725

不同成熟度的阿森泰克苹果品质变化大,会显著影响采后贮藏与销售效益。本研究以江苏宿迁四个成熟阶段的阿森泰克苹果为研究对象,首先利用主成分分析(principal component analysis,PCA)和线性判别分析(linear discriminant analysis,LDA... 详细信息

不同成熟度的阿森泰克苹果品质变化大,会显著影响采后贮藏与销售效益。本研究以江苏宿迁四个成熟阶段的阿森泰克苹果为研究对象,首先利用主成分分析(principal component analysis,PCA)和线性判别分析(linear discriminant analysis,LDA)分析其色泽(L^(*)、a^(*)、b^(*)值)、硬度(firmness,FI)、可溶性固形物(soluble solid content,SSC)、可滴定酸(titratable acidity,TA)、水分含量(moisture content,MC)、干物质(dry matter content,DMC)的变化规律;同时,基于可见-近红外(visible and near-infrared,Vis-NIR)与近红外(nearinfrared,NIR)光谱技术结合连续投影(successiveprojectionsalgorithm,SPA)、竞争性自适应重加权(competitive adaptive reweighted sampling,CARS)、无信息变量消除(uninformative variable elimination,UVE)算法进行相关特征变量筛选,基于偏最小二乘(partial least squares,PLS)与支持向量机(support vector machine,SVM)建立阿森泰克苹果品质预测模型。结果表明,SSC、a^(*)、L^(*)、b^(*)对不同成熟度阿森泰克苹果的聚类贡献率较高,510~680、1170~1270、2300 nm为高相关度特征波段。SPA-PLS、SPA-SVM模型能很好地预测不同成熟度阿森泰克的L^(*)、b^(*)、a^(*)值,相对预测偏差(relative percent deviation,RPD)均高于3.00,CARS-PLS模型可以很好地预测SSC,RPD为3.19,但FI、TA、MC、DMC的SPA-PLS模型预测精度相对较低,RPD分别为2.27、2.21、2.32、2.42。研究结果证明Vis-NIR和NIR光谱方法能够预测不同成熟度阿森泰克苹果品质,为阿森泰克苹果采收管理与质量安全控制提供技术参考。

关键词：阿森泰克苹果可见-近红外光谱近红外光谱成熟度品质

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温度、盐度和密度对菲律宾蛤仔稚贝生长和存活的影响

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中国海洋大学学报（自然科学版） 2024年第1期54卷 58-66页

作者：吴磊涂康周丽青刘志鸿吴彪孙秀俊中国水产科学研究院黄海水产研究所山东青岛266071 青岛海洋科学与技术试点国家实验室海洋渔业科学与食物产出过程功能实验室山东青岛266237 江苏海洋大学海洋科学与水产学院江苏连云港222005 莆田市水产研究所福建莆田351100

为查明温度、盐度和密度对菲律宾蛤仔(Ruditapes philippinarum)稚贝生长和存活的影响,本研究通过响应面法构建了不同环境条件下蛤仔生长存活模型并进行分析。实验设计了3因素(温度、盐度、密度)和3水平(-1、0、1),以稚贝壳长为响应值,... 详细信息

为查明温度、盐度和密度对菲律宾蛤仔(Ruditapes philippinarum)稚贝生长和存活的影响,本研究通过响应面法构建了不同环境条件下蛤仔生长存活模型并进行分析。实验设计了3因素(温度、盐度、密度)和3水平(-1、0、1),以稚贝壳长为响应值,探究温度、盐度、密度三因素及其相互作用对蛤仔稚贝生长和存活的影响。研究表明,培育73 d后,温度、盐度、密度的二次效应对蛤仔稚贝的壳长生长影响极显著(P0.05)。壳长预测值Y对应自变量温度(T)、盐度(S)和密度(D)的二次多项回归方程:Y=-6.33105+0.2765 T+0.573 S+0.689 D-0.00566 T^(2)-0.0137 S^(2)-0.1159 D^(2)。本研究通过二次响应面回归方程构建了不同环境下蛤仔苗种的生长模型,得出稚贝最适生长环境为温度24.32℃、盐度21.13、密度2.88×10^(4)粒/m^(2),在此条件下蛤仔稚贝平均壳长可达到最大值(4.07 mm)。

关键词：菲律宾蛤仔温度盐度密度生长

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Drift and deposition of ultra-low altitude and low volume application in paddy field

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International Journal of Agricultural and Biological Engineering 2014年第4期7卷 23-28页

作者： Xue Xinyu tu kang Qin Weicai Yubin Lan Huihui Zhang Nanjing Agricultural University Nanjing 210014China Nanjing Research Institute for Agricultural Mechanization Ministry of AgricultureNanjing 210014China South China Agricultural University Guangzhou 510642China USDA-ARS Fort CollinsCA 80526USA

Field trials were performed to evaluate various techniques for measuring spray deposition and aerial drift during spray application to paddy *** application of a spraying agent containing the fluorescent dye Rhodamine-B was applied by an unmanned aerial vehicle(UAV)which flew at a height of 5 m,a speed of 3 m/s,and the wind speed of 3 m/*** results showed that because the downdraft produced by a helicopter rotor increased the penetrability of crops,there is a higher deposition on the upper layer and the under layer than the traditional *** average deposition on the upper layer accounts for 28% of the total spraying,the deposition on the under layer accounts for 26% of the total *** deposition on the under layer takes up 92.8% of the deposition on the upper *** drift data showed that the drift of non-target area took up 12.9% of the total liquid *** 90% drifting droplets were located within a range of 8 m of the target area;the drift quantity was almost zero at a distance of 50 m away from the treated area.

关键词： paddy field ultra-low altitude low volume UAV droplet drift deposition

Phage displayed domain antibody mimics for pyrethroid and its application in immunoassay

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International Journal of Agricultural and Biological Engineering 2020年第2期13卷 235-240页

作者： Yanyan Zhao Ying Liang Yuan Liu Sicong tu Shengming Zhao Xianjin Liu kang tu School of Food Science and technology Henan Institute of Science and TechnologyXinxiang 453003China Key Laboratory of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base Key Laboratory of Control Technology and Standard for Agri-product Safety and QualityMinistry of Agriculture and Rural AffairsInstitute of Agricultural Product Quality Safety and Nutrition ResearchJiangsu Academy of Agricultural SciencesNanjing 210014China College of Food Science and Technology Nanjing Agricultural UniversityNanjing 210095China School of Medical Sciences University of New South WalesSydneyNSW 2052Australia

Anti-idiotypic antibodies which can mimic antigens have many potential applications in the *** research used the monoclonal antibody of the conserved chemical region of pyrethroids,3-phenoxybenzoic acid(PBA),and the domain antibody library to develop an environmentally-friendly immunoassay for the detection of *** domain antibodies(A8,B8,and C6)which bound to anti-PBA monoclonal antibody(MAb)were isolated from a naive phage display human domain antibody *** domain antibody is cloneable,pyrethroid-free,and applicable as a competitive mimetic antigen in the *** best immunoassay was achieved using A8,resulting in IC50 of 0.714μg/mL for PBA,1.775μg/mL for Cypermethrin,1.624μg/mL forβ-cypermethrin,3.675μg/mL for Fenvalerate,and 4.895μg/mL for *** way of selecting anti-idiotypic antibodies to detect pyrethroids could provide potential applications in developing immunoassays for identifying various chemical contaminants in food.

关键词： domain antibody enzyme-linked immunosorbent assay phenoxybenzoic acid pyrethroid