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Potential bioremediation of mercury-contaminated substrate using filamentous fungi isolated from forest soil

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Journal of Environmental Sciences 2014年第6期26卷 1223-1231页

作者： Evi Kurniati Novi Arfarita tsuyoshi imai Takaya Higuchi Ariyo Kanno Koichi Yamamoto Masahiko Sekine Department of Agricultural Engineering Brawijaya University Division of Environmental Science and Sustainable Engineering Graduate School of Science and Engineering Yamaguchi University Department of Agrotechnology Malang Islamic University

The use of filamentous fungi in bioremediation of heavy metal contamination has been developed recently. This research aims to observe the capability of filamentous fungi isolated from forest soil for bioremediation of mercury contamination in a substrate. Six fungal strains were selected based on their capability to grow in 25 mg/L Hg2＋-contaminated potato dextrose agar plates. Fungal strain KRP1 showed the highest ratio of growth diameter, 0.831, thus was chosen for further observation. Identification based on colony and cell morphology carried out by 18S rRNA analysis gave a 98% match to Aspergillusflavus strain KRP1. The fungal characteristics in mercury（II） contamination such as range of optimum pH, optimum temperature and tolerance level were 5.5-7 and 25-35℃ and 100 mg/L respectively. The concentration of mercury in the media affected fungal growth during lag phases. The capability of the fungal strain to remove the mercury（II） contaminant was evaluated in 100 mL sterile 10 mg/L Hg2＋-contaminated potato dextrose broth media in 250 mL Erlenmeyer flasks inoculated with 108 spore/mL fungal spore suspension and incubation at 30℃ for 7 days. The mercury（II） utilization was observed for flasks shaken in a 130 r/min orbital shaker （shaken） and non- shaken flasks （static） treatments. Flasks containing contaminated media with no fungal spores were also provided as control. All treatments were done in triplicate. The strain was able to remove 97.50% and 98.73% mercury from shaken and static systems respectively. A.flavus strain KRPI seems to have potential use in bioremediation of aqueous substrates containing mercury（II） through a biosorption mechanism.

关键词： Aspergillusflavus strain KRP1 biosorption mercury contaminant

Potential application of high pressure carbon dioxide in treated wastewater and water disinfection:Recent overview and further trends

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Journal of Environmental Sciences 2015年第10期27卷 38-47页

作者： Huy Thanh Vo tsuyoshi imai Truc Thanh Ho Thanh-Loc Thi Dang Son Anh Hoang Division of Urban Infrastructural Engineering Mien trung University of Civil Engineering Division of Environmental Science and Engineering Yamaguchi University

Recently emerging disadvantages in conventional disinfection have heightened the need for finding a new solution. Developments in the use of high pressure carbon dioxide for food preservation and sterilization have led to a renewed interest in its applicability in wastewater treatment and water disinfection. Pressurized CO2 is one of the most investigated methods of antibacterial treatment and has been used extensively for decades to inhibit pathogens in dried food and liquid products. This study reviews the literature concerning the utility of CO2 as a disinfecting agent, and the pathogen inactivation mechanism of CO2 treatment is evaluated based on all available research. In this paper, it will be argued that the successful application and high effectiveness of CO2 treatment in liquid foods open a potential opportunity for its use in wastewater treatment and water disinfection. The findings from models with different operating conditions（pressure, temperature, microorganism, water content, media …） suggest that most microorganisms are successfully inhibited under CO2 treatment. It will also be shown that the bacterial deaths under CO2 treatment can be explained by many different mechanisms.Moreover, the findings in this study can help to address the recently emerging problems in water disinfection, such as disinfection by-products（resulting from chlorination or ozone treatment）.

关键词： High pressure CO2 Inactivation effect Inactivation mechanism

Delignification of disposable wooden chopsticks waste for fermentative hydrogen production by an enriched culture from a hot spring

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Journal of Environmental Sciences 2014年第6期26卷 1361-1368页

作者： Kanthima Phummala tsuyoshi imai Alissara Reungsang Prapaipid Chairattanamanokorn Masahiko Sekine Takaya Higuchi Koichi Yamamoto Ariyo Kanno Division of Environmental Science and Engineering Graduate School of Science and Engineering Yamaguchi University Yamaguchi 7558611Japan Department of Biotechnology Faculty of Technology Khon Kaen University Khon Kaen 40000 Thailand Environmental Technology Unit Department of Environmental Science Kasetsart University Bangkok 10903 Thailand Division of Civil and Environmental Engineering Graduate School of Science and Engineering Yamaguchi University Yamaguchi 7558611 Japan

Hydrogen （H2） production from lignocellulosic materials may be enhanced by removing lignin and increasing the porosity of the material prior to enzymatic hydrolysis. Alkaline pretreatment conditions, used to delignify disposable wooden chopsticks （DWC） waste, were investigated. The effects of NaOH concentration, temperature and retention time were examined and it was found that retention time had no effect on lignin removal or carbohydrate released in enzymatic hydrolysate. The highest percentage of lignin removal （41%） was obtained with 2% NaOH at 100℃, correlated with the highest carbohydrate released （67 mg/gpretreated DWC） in the hydrolysate. An enriched culture from a hot spring was used as inoculum for fermentative H2 production, and its optimum initial pH and temperature were determined to be 7.0 and 50℃, respectively. Furthermore, enzymatic hydrolysate from pretreated DWC was successfully demonstrated as a substrate for fermentative H2 production by the enriched culture. The maximum H2 yield and production rate were achieved at 195 mL H2/g total sugarsconsumed and 1 16 mL Hz/（L.day）, respectively.

关键词： alkaline pretreatment disposable wooden chopsticks waste fermentative hydrogen production hot spring enriched culture

Response surface method for modeling the removal of carbon dioxide from a simulated gas using water absorption enhanced with a liquid-film-forming device

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Journal of Environmental Sciences 2018年第3期30卷 116-126页

作者： Diem-Mai Kim Nguyen tsuyoshi imai Thanh-Loc Thi Dang Ariyo Kanno Takaya Higuchi Koichi Yamamoto Masahiko Sekine Graduate School of Environmental Sciences and Engineering Yamaguchi University Graduate School of Sciences and Technology for Innovation Yamaguchi University Department of Environmental Science College of Sciences Hue University

This paper presents the results from using a physical absorption process to absorb gaseous CO2mixed with N2using water by producing tiny bubbles via a liquid-film-forming device（LFFD）that improves the solubility of CO2in water.The influence of various parameters—pressure,initial CO2concentration,gas-to-liquid ratios,and temperature—on the CO2removal efficiency and its absorption rate in water were investigated and estimated thoroughly by statistical polynomial models obtained by the utilization of the response surface method（RSM）with a central composite design（CCD）.Based on the analysis,a high efficiency of CO2capture can be reached in conditions such as low pressure,high CO2concentration at the inlet,low gas/liquid ratio,and low temperature.For instance,the highest removal efficiency in the RSM–CCD experimental matrix of nearly 80%occurred for run number 20,which was conducted at 0.30 MPa,CO2concentration of 35%,gas/liquid ratio of 0.71,and temperature of 15℃.Furthermore,the coefficients of determination,R^2,were 0.996 for the removal rate and 0.982 for the absorption rate,implying that the predicted values computed by the constructed models correlate strongly and fit well with the experimental values.The results obtained provide essential information for implementing this method properly and effectively and contribute a promising approach to the problem of CO2capture in air pollution treatment.

关键词： Carbon dioxide Water dissolution Liquid-film Response surface method （RSM）

Inactivation effect of pressurized carbon dioxide on bacteriophage Qβ and ΦX174 as a novel disinfectant for water treatment

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Journal of Environmental Sciences 2014年第6期26卷 1301-1306页

作者： Huy Thanh Vo tsuyoshi imai Truc Thanh Ho Masahiko Sekine Ariyo Kanno Takaya Higuchi Koichi Yamamoto Hidenori Yamamoto Faculty of Engineering Yamaguchi University 2-16-1 TokiwadaiYamaguchi 755-8611 Japan Faculty of Urban Engineering Central University of ConstructionTuy Hoa Phu Yen 56000 Vietnam

The inactivation effects of pressurized CO2 against bacteriophage Qβ and ФX 174 were investigated under the pressure of 0.3-0.9 MPa, initial concentration of 107-109 PFU/mL, and temperature of 17.8℃-27.2℃. The optimum conditions were found to be 0.7 MPa and an exposure time of 25 min. Under identical treatment conditions, a greater than 3.3-log reduction in bacteriophage Qβ was achieved by CO2, while a nearly 3.0 log reduction was observed for phage ФX174. The viricidal effects of N2O （an inactivation gas with similar characteristics to CO2）, normal acid （HC1）, and CO2 treatment with phosphate buffered saline affirmed the chemical nature of CO2 treatment. The pumping cycle, depressurization rate, and release of intracellular substances caused by CO2 were its viricidal mechanisms. The results indicate that CO2 has the potential for use as a disinfectant without forming disinfection by-products.

关键词： bacteriophage Qβ bacteriophage ФX174 pressurized CO2 viricidal effect microbubbles water disinfection

γ-Butyrolactone Synthesis from Allylic Alcohols Using the CO_(2)Radical Anion

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Precision Chemistry

Precision Chemistry 2024年第3期2卷 88-95页

作者： Saeesh R.Mangaonkar Hiroki Hayashi Wataru Kanna Suvankar Debbarma Yu Harabuchi Satoshi Maeda tsuyoshi Mita Institute for Chemical Reaction Design and Discovery(WPI-ICReDD) Hokkaido UniversitySapporoHokkaido 001-0021Japan JST ERATO Maeda Artificial Intelligence in Chemical Reaction Design and Discovery ProjectSapporoHokkaido 060-0810Japan Department of Chemistry Faculty of ScienceHokkaido UniversitySapporoHokkaido 060-0810Japan Research and Services Division of Materials Data and Integrated System(MaDIS) National Institute for Materials Science(NIMS)TsukubaIbaraki 305-0044Japan

γ-Butyrolactone structures are commonly found in various natural products and serve as crucial building blocks in organic synthesis.Consequently,the development of methods for synthesizingγ-butyrolactones has garnered significant interest within the organic synthesis community.In this study,we present a direct and highly efficient approach for the synthesis ofγ-butyrolactones from allylic alcohols.Notably,this study represents the first instance ofγ-butyrolactone synthesis initiated by radical hydrocarboxylation using CO_(2)^(•–),generated from metal formates,followed by cyclization.This two-step process is achieved through the synergistic interaction of photoredox and hydrogen atom transfer(HAT)catalysis,resulting in the production ofγ-butyrolactones with exceptional efficiency.Additionally,when employingα,α-diaryl allylic alcohol derivatives as substrates,the reaction involves 1,2-aryl migration,which occurs concomitantly with CO_(2)^(•–)addition,leading to the formation of 4,5-substituted lactones in a good yield.The artificial force induced reaction(AFIR)method identified the preferred 1,2-aryl migration pathway along with potential byproduct pathways,in which the targeted 1,2-migration was found to be the most plausible pathway.

关键词： CO_(2) lactone radical photoredox visible light carboxylation calculation DFT

Preoperative albumin-bilirubin score and liver resection percentage determine postoperative liver regeneration after partial hepatectomy

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World Journal of Gastroenterology 2024年第14期30卷 2006-2017页

作者： Kazuhiro Takahashi Masahiko Gosho Yoshihiro Miyazaki Hiromitsu Nakahashi Osamu Shimomura Kinji Furuya Manami Doi Yohei Owada Koichi Ogawa Yusuke Ohara Yoshimasa Akashi tsuyoshi Enomoto Shinji Hashimoto Tatsuya Oda Department of Gastrointestinal and Hepatobiliary-Pancreatic Surgery University of TsukubaTsukuba 3058-575IbarakiJapan Department of Biostatistics University of TsukubaTsukuba 3058-575IbarakiJapan

BACKGROUND The success of liver resection relies on the ability of the remnant liver to regenerate.Most of the knowledge regarding the pathophysiological basis of liver regeneration comes from rodent studies,and data on humans are scarce.Additionally,there is limited knowledge about the preoperative factors that influence postoperative regeneration.AIM To quantify postoperative remnant liver volume by the latest volumetric software and investigate perioperative factors that affect posthepatectomy liver regenera-tion.METHODS A total of 268 patients who received partial hepatectomy were enrolled.Patients were grouped into right hepatectomy/trisegmentectomy(RH/Tri),left hepa-tectomy(LH),segmentectomy(Seg),and subsegmentectomy/nonanatomical hepatectomy(Sub/Non)groups.The regeneration index(RI)and late rege-neration rate were defined as(postoperative liver volume)/[total functional liver volume(TFLV)]×100 and(RI at 6-months-RI at 3-months)/RI at 6-months,respectively.The lower 25th percentile of RI and the higher 25th percentile of late regeneration rate in each group were defined as“low regeneration”and“delayed regeneration”.“Restoration to the original size”was defined as regeneration of the liver volume by more than 90%of the TFLV at 12 months postsurgery.RESULTS The numbers of patients in the RH/Tri,LH,Seg,and Sub/Non groups were 41,53,99 and 75,respectively.The RI plateaued at 3 months in the LH,Seg,and Sub/Non groups,whereas the RI increased until 12 months in the RH/Tri group.According to our multivariate analysis,the preoperative albumin-bilirubin(ALBI)score was an independent factor for low regeneration at 3 months[odds ratio(OR)95%CI=2.80(1.17-6.69),P=0.02;per 1.0 up]and 12 months[OR=2.27(1.01-5.09),P=0.04;per 1.0 up].Multivariate analysis revealed that only liver resection percentage[OR=1.03(1.00-1.05),P=0.04]was associated with delayed regeneration.Furthermore,multivariate analysis demonstrated that the preoperative ALBI score[OR=2.63(1.00-1.05),P=0.02;per 1.0 up]and liver resection percentage[OR=1.02(1.00

关键词： Liver regeneration Albumin-bilirubin score Liver resection percentage Partial hepatectomy Human Regeneration index

Malnutrition and Its Association with the Mortality of Patients with Femoral Intertrochanteric Fractures: A Retrospective Analysis of Japanese Patients

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Open Journal of Orthopedics 2024年第1期14卷 22-31页

作者： Taizo Kaneko Kyoko Matsudaira Kentaro Hayakawa Fumiaki Tokimura tsuyoshi Miyazaki Department of Orthopaedic Surgery Tokyo Metropolitan Institute for Geriatrics and Gerontology Tokyo Japan

Background: The incidence of femoral intertrochanteric fractures in older adults is higher than that of femoral neck fractures;however, both conditions are often analyzed together as proximal femoral fractures. Considering the difference in treatment, postoperative complication, and mortality risk, these two fractures should be analyzed separately. This study aimed to analyze 1-year mortality and its risk factors in patients with surgically treated femoral intertrochanteric fractures. Methods: Consecutive patients with intertrochanteric fractures who underwent surgical interventions at our institution between January 2017 and December 2021 were retrospectively reviewed. A total of 238 patients were eligible for inclusion in this study. Patients’ demographic and clinical information were retrospectively collected. Patients were divided into the 1-year mortality (n = 16) and survival (n = 222) groups. The incidence of 1-year mortality and its independent risk factors were investigated using univariate and multivariate logistic regression analyses. Results: The mean age of patients was 85.6 ± 8.5 years. The 1-year mortality rate was 6.7% (16/238). Preoperative albumin level, the Geriatric Nutritional Risk Index (GNRI), and malnutrition status (GNRI p = 0.02, p = 0.02, and p = 0.0011, respectively). Multivariate analysis showed that malnutrition status (GNRI p = 0.035) was an independent risk factor for 1-year mortality. Conclusion: Malnutrition status assessed using GNRI (GNRI < 92) was an independent risk factor for 1-year mortality. Our findings suggest that GNRI may be an effective screening tool for predicting postoperative 1-year mortality of patients with surgically treated femoral intertrochanteric fractures.

关键词： Intertrochanteric Fracture Mortality Geriatric Nutritional Risk Index Malnutrition Risk Factor

Biosynthesis of Polyhydroxyalkanoate （PHA） by Hydrogenophaga sp. Isolated from Soil Environment during Batch Fermentation

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Journal of Life Sciences 2011年第12期5卷 1003-1012页

作者： Varavut Tanamool tsuyoshi imai Paiboon Danvirutai Pakawadee Kaewkannetra Department of Bioteehnology Graduate School of Khon Kaen University Khon Kaen 40002 Thailand Division of Environmental Science and Engineering Graduate School of Science and Engineering Yamaguchi UniversityTokiwadai Ube Yamaguchi 755-8611 Japan Fermentation Research Center for Value Added Agricultural Products （FerVAAP） Faculty of Technology Khon Kaen UniversityKhon Kaen 40002 Thailand Department of Bioteehnology Faculty of Technology Khon Kaen University Khon Kaen 40002 Thailand

In this work, sucrose utilizing microbes from soil were screened to evaluate their ability for accumulation of biopolymer of polyhydroxyalkanoate (PHA). Among 72 isolates were transferred to mineral salt medium (MSM), 33 strains can be grown on sucrose agar medium. However, only one strain showed a strong black color for Sudan Black and gave positive result for Nile blue A. Identification by 16S rDNA nucleotide sequence homology of the isolate showed very closely to Hydrogenophaga sp. (99% identify). To consider PHA production, the isolate was grown in the medium containing sucrose as a sole carbon under controlled conditions of 35 °C and at pH 7. Maximum dry cell weight (DCW) and PHA production were obtained at 3.61 g/L and 2.41 g/L after 36 and 42 h batch fermentation. Sucrose uptake measured in term of total organic carbon (TOC) showed at 14.73 g within 48 h. The highest PHA was 68.15% (gPHA/gDCW) giving maximum PHA yield (YP/S) of 0.17 (gPHA/gSucrose) and a productivity of 0.057 gPHA/L·h. This highlights the potential of microbial resources in soil environment and may be an exploitable application for the industrial production of PHA.

关键词：聚羟基脂肪酸酯分批发酵过程土壤环境分离鉴定 PHA 生物合成 SP 琼脂培养基