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Constitutive Overexpression of Bcl-2, Survivin and ER Stress Chaperone GRP-78 Confers Intrinsic Radioresistance in Human Hepatocellular Carcinoma Cells: Insight into the Mechanistic Pathways Involved

第九次全国生物物理大会学术会议

作者： tsan-zon liu School of Medical Technology and Graduate Institute of Medical Biotechnology Chang Gung UniversityKwei-ShanTaoyuanTaiwan

In the interest of developing a simple and rapid ultraweak chemiluminescence assay for assessing thesuperoxide (O)-scavenging activities of various aqueous extracts of food constituents, a specific and stableO-generating system was sought. Reported herein is the obtainment for the first time of a specific andstable O-generating system consisting of methylglyoxal (MG), a reactive 2- oxo- aldehyde and arginine,

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Journal of Cancer Therapy 2013年第2期4卷 399-407页

作者： Chih-Hsiung Wu Yih-Huei Uen Chun-Te Ho Ya-Ting Tseng tsan-zon liu Jeng-Fong Chiou Stephen Wan Leung Cancer Center and Department of Radiation Oncology Taipei Medical University and Hospital Taipei Chinese Taipei Department of General Surgery Chi-Mei Foundation Medical Center Tainan Chinese Taipei Department of Radiology and Nuclear Medicine Yuan’s General Hospital Kaohsiung Chinese Taipei Department of Surgery Taipei Medical University-Shuang Ho Hospital Taipei Chinese Taipei Graduate Institute of Medical Biotechnology Chang-Gung University Gueishan Chinese Taipei Graduate Institute of Medical Sciences Taipei Medical University Taipei Chinese Taipei

We present evidence here that abundantly expressed b-catenin-triggered NF-kB-dependent upregulation of inducible nitric oxide synthase(iNOS) found in hepatoma Mahlavu cells (RT-resistant variant designated as RR-Mal), but not in Hep 3B cells (RT-sensitive variant designated as RS-3B) is a key element contribrting to the radioresisitance through the activation of two prominent radioprotective pathways. First, high iNOS expression found in RR-Mal, but not in RS-3B cells was found to perturb calcium homeostasis that triggered ER stress response leading to the overproduction of ER chaperone GRP-78 via robust generation of cleaved ATF-6a (50 kDa) subunits and their nuclear translocation. Meanwhile, both abundantly expressed NF-κB and COX-2 found in RR-Mal cells could also provoke an increased production of PGE2 resulting in robust production of Bcl-2. Interestingly, when RR-Mal cells were treated with PDTC (a NF-κB inhibitor) or celecoxib (a COX-2 inhibitor), a concentration-dependent downregulation of Bcl-2 could be demonstrated implying that Bcl-2 overexpression was indeed mediated through NF-κB/Cox-2/PGE2 pathway. Importantly, we also unveiled that siRNA-mediated silencing of survivin in RR-Mal cells could result in a concomitant downregulation of GRP-78 due to a severe inhibition of ATF-6a (50 kDa) expression. Taken together, our data demonstrate that constitutively overexpressed b-catenin/NF-κB/iNOS and NF-κB/COX-2/PGE2 pathways that overproducing GRP-78, survivin and Bcl-2 expressions are responsible for radioresistance acquisition in RR-Mal cells. Thus, both pathways could be served as potential targets for overcoming radioresistance.

关键词： Radioresistance Hepatoma Cells Inducible Nitric Oxide Synthase Bcl-2 Survivin GRP-78

A Novel Probe-Based Ultraweak Chemiluminescence Technique for the Detection of A Set of Four Oxygen-Derived Free Radicals and Their Application in Assessing Radical-Scavenging Abilities of Antioxidant Mixtures or Purified Compounds

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A Novel Probe-Based Ultraweak Chemiluminescence Technique fo...

OVOL1 inhibits breast cancer cell invasion by enhancing the degradation of TGF-β type I receptor

第六届全国自由基生物学与自由基医学学术会议和海峡两岸自由基生物学与自由基医学学术会议

作者： Chin-Hung Tsai, Chih-Wei Chen and tsan-zon liu Department of Food Nutrition, Chung-Hwa Institute of Technology, Tainan Center for Gerontological Research and Graduate Institute of Medical Biotechnology, Chang Gung University, Kwei-Shan, Taoyuan

A probe-based ultraweak chemiluminescence (CL) technique capable of measuring the production of a set of four oxygen derived free radicals performed by same CL analyzer is described. First, the selected radical-generating system and their corresponding CL-probing emitters are: (1) methylglyoxal + arginine/lucigenin for superoxide (O2-);(2) H2O2 + Fe2+ (Fenton reagent)/indoxyl-β-glucuronide (IBG) for hydroxyl radical ( OH);(3) 2, 2’- A/o- bis (2-amidinopropane) (ABAP)/luminal for peroxyl radical (ROO ) and H2O2/luminal for hydrogen peroxide [a radical-producing precursor or reactive oxygen species (ROS)]. Second, this methodology has been demonstrated to be versatile for the assessment of radical-scavenging abilities of food extracts, herbal preparations as well as purified antioxidant compounds. Finally, a specific example for assessing radical-scavenging abilities of O2-, H2O2,·OH and ROO by aqueous extracts of Cordycepts sinensis is given to illustrate its practical applications.

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Signal Transduction and Targeted Therapy 2022年第5期7卷 1842-1859页

作者： Chuannan Fan Qian Wang Gerard van der zon Jiang Ren Cedrick Agaser Roderick C.Slieker Prasanna Vasudevan Iyengar Hailiang Mei Peter ten Dijke Department of Cell and Chemical Biology Leiden University Medical CenterLeiden 2300 RCThe Netherlands Oncode Institute Leiden University Medical CenterLeiden 2300 RCThe Netherlands Department of Biomedical Data Sciences Sequencing Analysis Support CoreLeiden University Medical CenterLeiden 2300 RCThe Netherlands Department of Epidemiology and Data Science Amsterdam Public Health InstituteAmsterdam Cardiovascular Sciences InstituteAmsterdam UMClocation VUmcAmsterdam 1081 HVThe Netherlands

Ovo-like transcriptional repressor 1(OVOL1)is a key mediator of epithelial lineage determination and mesenchymal–epithelial transition(MET).The cytokines transforming growth factor-β(TGF-β)and bone morphogenetic proteins(BMP)control the epithelial–mesenchymal plasticity(EMP)of cancer cells,but whether this occurs through interplay with OVOL1 is not ***,we show that OVOL1 is inversely correlated with the epithelial–mesenchymal transition(EMT)signature,and is an indicator of a favorable prognosis for breast cancer ***1 suppresses EMT.

关键词： invasion breast cancer

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应变软化对复合材料分层尖端场的影响

计算力学学报 2006年第3期23卷 357-362页

作者：刘军李英梅张凤鹏黄宝宗东北大学理学院辽宁沈阳110004

提出了适用于纤维增强复合材料应变软化分析的多标量连续损伤模型,并将其应用于含分层裂纹的复合材料层板的后屈曲损伤破坏有限元分析,研究了软化参数对损伤场和应力场的影响。计算结果表明:(1)应变软化使分层尖端的应力奇异性降低或消... 详细信息

提出了适用于纤维增强复合材料应变软化分析的多标量连续损伤模型,并将其应用于含分层裂纹的复合材料层板的后屈曲损伤破坏有限元分析,研究了软化参数对损伤场和应力场的影响。计算结果表明:(1)应变软化使分层尖端的应力奇异性降低或消失。(2)应变软化参数影响极限损伤区的大小及其扩展速度,应力跌落使极限损伤加剧。(3)由于损伤的影响,裂尖的能量释放率受软化参数的影响出现波动,已难反映分层特征,可以根据损伤程度来判断分层的扩展。(4)分层上下表面的纤维方向影响损伤形式及其扩展方向。

关键词：应变软化损伤裂尖奇异性复合材料层板能量释放率

论马克思的异化理论研究与唯物史观确立的关系

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凯里学院学报 2009年第2期27卷 1-8页

作者：刘宗碧凯里学院价值学经济哲学研究所贵州凯里556011

关于马克思异化理论与唯物史观的关系,目前国外学界主要形成了两种不同的观点:一是"顶峰论",二是"最远论",都否认了异化理论对唯物史观确立的作用。我国学界把马克思异化理论当作仍未脱胎于黑格尔、费尔巴哈框架的不成熟作品,从而不太... 详细信息

关于马克思异化理论与唯物史观的关系,目前国外学界主要形成了两种不同的观点:一是"顶峰论",二是"最远论",都否认了异化理论对唯物史观确立的作用。我国学界把马克思异化理论当作仍未脱胎于黑格尔、费尔巴哈框架的不成熟作品,从而不太注重马克思异化理论与黑格尔、费尔巴哈的区别,相反地倾向于注重他们的联系,从而未能对异化理论与唯物史观学理关联的深入揭示。其实,在理论过渡上,马克思的异化理论与唯物史观是有深刻联系的,并且对唯物史观的确立有着重大的作用。这个作用主要是在变革近代哲学主题与范式上,通过"主体"的科学重构,克服西方传统认识论上"主客观同一性"论证的局限,使之转换和上升为本体论与认识论相统一的"主客体同一性"证明,建立了以融合了主客观的价值规律为内容的理论对象,最终完成真理观与价值观的统一,为进一步与历史观的统一和通向唯物史观奠定了基础。马克思异化理论研究是唯物史观学理建构的必要环节,是唯物史观确立不可或缺的重要组成部分。

关键词：马克思异化理论唯物史观真理观与价值观的统一

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现代工程训练中心建设及训练模式的探索

实验技术与管理 2009年第3期26卷 115-118页

作者：惠记庄刘海明邹亚科长安大学实验室管理处

如何建设现代工程训练中心,以及制定人才培养方案,充分发挥现代工程训练中心在21世纪高级工程人才培养中的作用,己成为高等工科教育研究的热点问题之一。结合作者在工程训练中心多年的管理心得,分析了基于大工程、大制造背景下的现代工... 详细信息

如何建设现代工程训练中心,以及制定人才培养方案,充分发挥现代工程训练中心在21世纪高级工程人才培养中的作用,己成为高等工科教育研究的热点问题之一。结合作者在工程训练中心多年的管理心得,分析了基于大工程、大制造背景下的现代工程训练中心的建设模式以及训练模式。在工程训练中心建设方面,围绕大工程、大制造工业环境的建立,教学模式改革,教师资源配置方案以及相关的人才培养机制,创新平台的建立,提出了一整套方案。在训练模式方面,以现代工程能力培养为目标,提出了4个层次金字塔型结构的工程训练体系。

关键词：工程能力训练中心建设训练模式

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激光相变硬化后的残余奥氏体分析

机械工程材料 1989年第2期13卷 35-38页

作者：刘江龙邹至荣重庆大学

本文对GCr15轴承钢在激光超快速加热淬火条件下得到的残余奥氏体特征进行了探讨。实验结果表明:在激光超快速加热淬火条件下,其残余奥氏体常常分布在未溶碳化物周围和马氏体组织之间,并呈薄膜盘状分布。这个常规热处理中的韧性相在激光... 详细信息

本文对GCr15轴承钢在激光超快速加热淬火条件下得到的残余奥氏体特征进行了探讨。实验结果表明:在激光超快速加热淬火条件下,其残余奥氏体常常分布在未溶碳化物周围和马氏体组织之间,并呈薄膜盘状分布。这个常规热处理中的韧性相在激光相变硬化中,被一定程度强化。这种形态和被超细化了的残余奥氏体对提高整个硬化效果和延迟马氏体之间和碳化物周圆界面处的显微裂纹的形核及其扩展极为有利。它对于强化层的韧化有极大贡献,为马氏体和碳化物组织的超高强韧性提供了又一可能的途径。

关键词：轴承钢激光相变硬化残余奥氏体

Targeting TGFβ signal transduction for cancer therapy

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Signal Transduction and Targeted Therapy 2021年第2期6卷 277-296页

作者： Sijia liu Jiang Ren Peter ten Dijke Oncode Institute and Department of Cell and Chemical Biology Leiden University Medical CenterEinthovenweg 202300 RC LeidenThe Netherlands

Transforming growth factor-β(TGFβ) family members are structurally and functionally related cytokines that have diverse effects on the regulation of cell fate during embryonic development and in the maintenance of adult tissue *** of TGFβ family signaling can lead to a plethora of developmental disorders and diseases,including cancer,immune dysfunction,and *** this review,we focus on TGFβ,a well-characterized family member that has a dichotomous role in cancer progression,acting in early stages as a tumor suppressor and in late stages as a tumor *** functions of TGFβ are not limited to the regulation of proliferation,differentiation,apoptosis,epithelial-mesenchymal transition,and metastasis of cancer *** reports have related TGFβ to effects on cells that are present in the tumor microenvironment through the stimulation of extracellular matrix deposition,promotion of angiogenesis,and suppression of the ant-tumor immune *** pro-oncogenic roles of TGFβ have attracted considerable attention because their intervention provides a therapeutic approach for cancer ***,the critical function of TGFβin maintaining tissue homeostasis makes targeting TGFβa ***,we review the pleiotropic functions of TGFβin cancer initiation and progression,summarize the recent clinical advancements regarding TGFβ signaling interventions for cancer treatment,and discuss the remaining challenges and opportunities related to targeting this *** provide a perspective on synergistic therapies that combine anti-TGFβ therapy with cytotoxic chemotherapy,targeted therapy,radiotherapy,or immunotherapy.

关键词： TGFβ homeostasis stimulation

南方稀土矿区马尾松树木年轮稀土元素含量年代变化

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稀土 2019年第6期40卷 66-73页

作者：张巧玲陈志强陈志彪潘宗涛冯柳俊湿润亚热带山地生态国家重点实验室培育基地福建福州350007 福建师范大学地理科学学院福建福州350007

为探讨稀土元素在马尾松树木年轮中的含量变化,以南方稀土矿区马尾松树木年轮为样本,通过ICPMS测定1997-2016年中La-Y共计15种稀土元素含量。结果表明,1997-2016年,单个稀土元素含量与稀土元素总量(ΣREE)除个别年份略有下降外,总体上... 详细信息

为探讨稀土元素在马尾松树木年轮中的含量变化,以南方稀土矿区马尾松树木年轮为样本,通过ICPMS测定1997-2016年中La-Y共计15种稀土元素含量。结果表明,1997-2016年,单个稀土元素含量与稀土元素总量(ΣREE)除个别年份略有下降外,总体上均波动上升,这可能是由于长汀在治理水土流失过程中,生长条件得到改善,促进了马尾松树木年轮对稀土元素的吸收;轻稀土元素总量(ΣLREE)与重稀土元素总量(ΣHREE)出现分布上的分异情况,且Eu元素明显亏损,Ce元素无明显异常,这与该地土壤中轻、重稀土元素的分异以及Eu元素的异常情况保持一致,并与该地区处于亚热带湿润气候区及淋溶作用强密不可分。而Ce元素无明显异常,有待进一步研究。同一树木年轮不同宽度下,稀土元素含量呈波动上升趋势,且同一树木年轮在不同宽度下稀土元素含量差距逐渐扩大,表明随着治理成效的逐渐显现,气候因素也对马尾松吸收稀土元素产生一定影响。

关键词：马尾松树木年轮稀土元素分布特征