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Anatomic variability of the vascularized composite osteomyocutaneous fl ap from the medial femoral condyle: an anatomical study

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Plastic and Aesthetic Research 2014年第1期1卷 85-88页

作者： trung-hau lêthua Duc-Phu Bui Dang-Nhat Pham Vu-Bao lê Albert De Mey Willy Boeckx Department of Plastic Reconstructive and Hand SurgeryHue Central HospitalHue City 47000Thừa Thiên-HuếVietnam Departments of Surgery Hue University of Medicine and PharmacyHue City 47000Thừa Thiên-HuếVietnam Department of Surgery An Sinh HospitalHo Chi Minh City 70000Vietnam Department of Plastic Surgery Brugmann University HospitalFree University of BrusselsBrussels 1020Belgium

Aim:The anatomical study and clinical application for the vascularized corticoperiosteal fl ap from the medial femoral condyle have been performed and described *** prior studies have described the composite osteomyocutaneous fl ap from the medial femoral condyle,a detailed analysis of the vascularity of this region has not yet been fully ***:This anatomical study described the variability of the arteries from the medial femoral condyle in 40 cadaveric ***:The descending genicular artery(DGA)was found in 33 of 40 cases(82.5%).The superomedial genicular artery(SGA)was present in 10 cases(25%).All 33 cases(100%)of the DGA had articular branches to the periosteum of the medial femoral *** branches and saphenous branches of the DGA were present in 25 cases(62.5%)and 26 cases(70.3%),***:The current study demonstrates that the size and length of the vessels to the medial femoral condyle are suffi cient for a vascularized bone fl ap.A careful preoperative vascular assessment is essential prior to use of the vascularized composite osteomyocutaneous fl ap from the medial femoral condyle,because of the considerable anatomical variations in different branches of the DGA.

关键词： Descending genicular artery medial femoral condyle osteomyocutaneousﬂap superomedial genicular artery

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Tissue engineering using mesenchymal stem cells with periosteal wrap for bone defect repair in rabbits

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Plastic and Aesthetic Research 2015年第1期2卷 340-345页

作者： trung-hau lêthua Dang-Nhat Pham Khanh-linh lê Minh-Tuan lê Quang-Ton-Quyen Nguyen Phan-Huy Nguyen Ngoc-Vu Tran Ngoc-luong Nguyen Willy Boeckx Albert Demey Department of Plastic Reconstructive and Hand SurgeryCenter of Orthopaedic and Plastic SurgeryHue Central HospitalHue City 531120Vietnam Department of Hematology Hue Central HospitalHue City 531120Vietnam Department of Biology College of SciencesHue UniversityHue City 521120Vietnam Department of Plastic Surgery Brugmann University HospitalUniversitélibre de Bruxelles1020 BrusselsBelgium

Aim:Mesenchymal stem cells(MSCs)are an excellent potential source of cells for bone tissue engineering due to their excellent renewal ability and osteogenic differentiation *** study was designed to evaluate the bone formation properties of a demineralized cancellous bone scaffold seeded with MSCs,with or without periosteum,in a critical size bone defect model in ***:Rabbit culture-expanded bone marrow(BM)-MSCs were seeded onto a human demineralized cancellous bone(HDCB)*** defects measuring 15 mm in length were created in each radius.A total of 56 bone defects in 28 rabbits were randomly assigned to one of the 4 groups for scaffold implantation:Group 1:HDCB graft only;Group 2:periosteum-wrapped HDCB graft;Group 3:HDCB graft seeded with BM-MSCs and Group 4:periosteum-wrapped HDCB graft seeded with *** rabbits were sacrificed 12 weeks after surgery for gross observation,radiological assessment,histological analyses and biomechanical ***:New bone(NB)formation and bone healing were successfully achieved,both radiologically and histologically,on demineralized cancellous bone graft seeded with *** were improved when BM-MSCs were associated with ***:This study demonstrates that repair of bone defects in a rabbit model can be achieved through bone grafting using BM-MSCs,implanted on a demineralized cancellous bone *** formation of NB was optimized when combined with the preservation of periosteum at the site of injury.

关键词： Bone defects bone marrow bone tissue engineering mesenchymal stem cells periosteum

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Research to Produce Ethanol from Seaweed Biomass Cladophora sp

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材料科学与工程（中英文B版） 2013年第10期3卷 670-676页

作者： Vo Thanh trung Bui Minh ly le Nhu hau Nguyen Thanh Hang Nhatrang Institute of Technology Reasearch and Application Nha Trang Viet Nam Ha Noi University of Science and Technology Ha Noi Viet Nam

Biomass seaweed Cladophora sp. (BSC) included Cladphora socialis, Cladophora prolifera, Cladophora crupila, which was hydrolysised in dilute sulfuric acid and enzymatic hydrolysis of cellulase. BSC was treated by dilute sulfuric acid at concentration of 0, 0.5%, 1%, 2%, 3%, 4%, 5%, 6% (v/v) (v volume) in autoclave respectively at 121 °C at time 20, 40 and 60 minute, with the rate between seaweed and solution acid of 1/10(w/v) (w weight). Beside, BSC was pretreated in dilute sulfuric acid at 0.5%(v/v) at 121 °C in 20 minute, after it was treat at concentrations 0, 0.2, 0.4, 0.6, 0.8, 1 ml/g with the enzymatic hydrolysis consisted of cellulase in control (50 °C, pH 5.0, 24 h). The results showed that, total sugar contents of two methods are the same, but sugar content was varied by treatment time of heat. Rates of acid 4%; 5%, 6% (v/v) and 0.8; 1 ml/g have high sugar content within the range of 42.38-48.51 mg/ml. 400 ml of hydrolysis solution was fermented by Saccharomyces cerevisiae at 30 °C for 72 h. After fermentation, 5% ethanol solution was identified for the first 100 ml evaporate solution. BSC is easily hydrolyzed and fermented to ethanol, this shows excellent prospects as a potential feedstock for the production of bioethanol.

关键词：乙醇溶液刚毛藻生物质 Alcalase碱性蛋白酶海藻生产水解纤维素总糖含量

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