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机械分割的小鼠桑椹胚和囊胚的发育能力

东北农学院学报 1992年第3期23卷 293-299页

作者：王周吉 Alan trounson 东北农学院哈尔滨150030 澳大利亚莫那什大学

利用机械方法二等份分割小鼠的桑椹胚、早期囊胚和晚期囊胚,并在体外培养分割的半胚到晚期囊胚。将这些囊胚移植到假孕受体鼠子宫内以评价他们附植后发育的能力。在体外培养中发现,囊胚期分割的胚胎比桑椹胚期分割的胚胎有较高的成活率... 详细信息

利用机械方法二等份分割小鼠的桑椹胚、早期囊胚和晚期囊胚,并在体外培养分割的半胚到晚期囊胚。将这些囊胚移植到假孕受体鼠子宫内以评价他们附植后发育的能力。在体外培养中发现,囊胚期分割的胚胎比桑椹胚期分割的胚胎有较高的成活率。通过附植前胚胎细胞数量分析得知,囊胚期半胚的细胞数量大约是对照组的一半,但内细胞团细胞与整个胚胎细胞的数量比不变。虽然从半胚发育到囊胚期的胚胎附植率仅略低于对照组,但半胚形成胎儿的能力却显著低于全胚。组织学分析表明,附植后的这种低成活率现象是由于缺乏卵柱发育造成的,而此卵柱则与半胚中的内细胞团的细胞数量减少有关。移植到第3天假孕受体鼠中的半胚,附植后的发育情况明显地比移植到第4天受体中的好。

关键词：小鼠桑椹胚囊胚机械分割发育

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牛胚胎的非手术移植

中国畜牧兽医 1979年第1期 22-23页

作者：陈俊英 trounson Rowson

用手术法移植牛胚胎,其胚胎成活率超过60％,而通过阴道和子宫颈用非手术法移植的胚胎成活率则较低,大约在25％左右。其原因是由于使用非手术法引起子宫感染和胚胎从子宫排出。但是,Lawson等(1975)报告,在间情期(发情后6—9天)移植胚胎... 详细信息

用手术法移植牛胚胎,其胚胎成活率超过60％,而通过阴道和子宫颈用非手术法移植的胚胎成活率则较低,大约在25％左右。其原因是由于使用非手术法引起子宫感染和胚胎从子宫排出。但是,Lawson等(1975)报告,在间情期(发情后6—9天)移植胚胎可提高其成活率,此时牛的子宫肌肉活动消失,即使在

关键词：非手术移植日龄妊娠率受体塑料套牛胚胎胚胎成活率子宫感染手术法输精器

The Potential of Rat Inner Cell Mass and Fetal Neural Stem Cells to Generate Chimeras

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Zoological Research 2009年第2期30卷 158-164页

作者：郭继彤李雪峰 Shahnaz Fida 苟克勉 Nakisa Malakooti ZHANG Chun-fang John R Morrison Alan O trounson DU Zhong-tao CopyRat Pty Ltd Clayton Victoria 3168 Australia Monash Immunology and Stem Cell Laboratories Monash University Clayton Victoria 3800 Australia 华南师范大学生命科学学院广东广州510631 Monash Animal Service Building 41 Monash University Wellington Road Clayton Victoria 3800 Australia

The rat chimera is an important animal model for the study of complex human diseases. In the present study we evaluated the chimeric potential of rat inner cell masses （ICMs） and fetal neural stem （FNS） cells. In result, three rat chimeras were produced by day 5 （D5） Sprague-Dawley （SD） blastocysts injected with ICMs derived from day 6 （D6） and D5 Dark Agouti （DA） blastocysts; four rat chimeras had been generated by D5 DA blastocyst injected with D5 SD ICMs. For the requirement of gene modification, cultured rat inner cell mass cells were assessed to produce chimeras, but no chimeras were generated from injected embryos. The potential to generate chimeras from rFNS and transfected rFNS cells were tested, but no chimeric pups were produced. Only 2 of 41 fetuses derived from D5 DA blastocyst injection with SD LacZ transfected rFNS cells showed very low number of LacZ positive cells in the section. These results indicate that DA and SD rat ICMs arc able to contribute to chimeras, but their potential decreases significantly after culture in vitro （P〈0.05）, and rFNS cells only have the potential to contribute to early fetal development.

关键词： Rat chimeras Inner cell mass Rat fetal neural stem cells Blastocyst injection

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植入前胚胎21号染色体嵌合现象主要由二倍体受孕引起

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世界核心医学期刊文摘（妇产科学分册） 2006年第1期2卷 34-34页

作者： Katz-Jaffe M.G. trounson A.O. Cram D.S. 张剑萍 Colorado Center for Reproductive Medicine 799 E. Hampden Ave Englewood CO 80113 United StatesDr.

High rates of chromosomal mosaicism in human IVF embryos question the accuracy of preimplantation genetic diagnosis, and, with the majority of embryo transfers still resulting in no pregnancy, chromosomal mosaicism is likely to be a contributing factor to human IVF failure. The aim of this study was to investigate the origin and nature of chromosome 21 (Ch21) cell division errors in human IVF embryos. Design: Perform single cell Ch21 allelic profiling on human IVF embryos. Setting: Academic research environment. Patient(s): Women of advanced maternal age (>35 yrs) (n = 65) undergoing infertility treatment; and amniocytes/chorionic cells from trisomy 21 pregnancies (n = 28). Intervention(s): Cells were analyzed by single cell allelic profiling, Main Outcome Measure(s): The origin and nature of cell division errors. Result(s): The vast majority of Ch21 mosaic embryos (～ 80% ) originated from diploid conceptions. In contrast, all fetal trisomy 21 originated from aneuploid conceptions. Increasing maternal age was significantly associated with aneuploid conceptions, meiotic cell division error, and adverse pregnancy outcome (P < .05). The mean daily FSH dose that produced embryos with normal Ch21 cell division was significantly lower than the mean daily FSH dose that produced embryos with mitotic Ch21 cell division errors (P < .01) and embryos with meiotic cell division errors (P < .05). Conclusion(s): Chromosomal mosaicism of Ch21 in human IVF embryos predominantly originate from diploid conceptions. Further understanding of chromosomal mosaicism with respect to IVF parameters, such as daily FSH dose, may eventually lead to improvements in IVF outcomes.

关键词： 21号染色体植入前胚胎嵌合现象二倍体受孕染色体嵌合型胚胎遗传学诊断 21-三体绒毛膜细胞细胞分裂

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