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Improving.precision in the assessment of round cell numbers in human semen

Asian Journal of Androlog. 2010年第1期12卷 111-114页

作者： trevor g. cooper barbara hellenkemper Centre of Reproductive Medicine and Androlog. University of Miinster Domagkstrasse 11 D-48149 Miinster Germany

The aim of this study was to increase the precision of assessment of the number of round cells observed in the per- oxidase test for detection of seminal leukocytes （g.anulocytes）. The dilution of semen was reduced and the volume of suspension examined was increased for semen samples containing.between 0.6 and 6 million round cells per mL. A 1 ＋ 5 （1：6） dilution was compatible with measurable peroxidase activity and a sufficiently clear backg.ound for cell assessment. At this dilution, and with assessment of all 18 g.ids on both sides of the Neubauer-improved counting.chamber, only three of the 10 samples （nominal cell concentrations of 1.9 ×10^-6-3.3×10^-6 mL-1） presented 400 round cells or more. As lower seminal dilutions were incompatible with easy detection of round cells or their peroxidase reaction product, it was not pos- sible to provide precise measurements （sampling.error 5%） of the sug.ested lower reference limit of 1 ×10^-6 cells per mL. The results indicate that this poor precision of measuring.1×10^-6 round cells per mL could explain the discrepant reports on the acceptability of the cut-off values for leukocytospermia. Such reference limits need to be established with statistically sound methods.

关键词： cell number peroxidase precision round cells semen

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Mountant-induced loss of Shorr-stained blue sperm tails

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Asian Journal of Androlog. 2010年第2期12卷 278-280页

作者： barbara hellenkemper Anda Strose trevor g. cooper Centre of Reproductive Medicine and Androlog. of the University Miinster D-48149 Germany Department of Life Science and Eng.neering Saxion Hogescholen Enschede 7500 Netherlands

染色的 Shorr 在精液在精子的活动性上提供另外的词法信息，这被报导了，由在红、蓝的毛虫之间区分。与我们的平淡的方法(包含的增长幻灯片) ，我们是不能的证实这些观察。然而，如果幻灯片是不骑马的，在染色 Shorr 的精液油迹的红有... 详细信息

染色的 Shorr 在精液在精子的活动性上提供另外的词法信息，这被报导了，由在红、蓝的毛虫之间区分。与我们的平淡的方法(包含的增长幻灯片) ，我们是不能的证实这些观察。然而，如果幻灯片是不骑马的，在染色 Shorr 的精液油迹的红有色、蓝有色的精子尾巴的存在是明显的。仅仅在蓝 flag.llar 染色和无法移动之间的一个很弱的协会被观察。说 mountant 是否被使用应该被报导。

关键词：精子尾巴鞭毛染色损失诱导运动形态幻灯片蓝色

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Dimensions of human ejaculated spermatozoa in Papanicolaou- stained seminal and swim-up smears obtained from the Integ.ated Semen Analysis System （ISAS）

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Asian Journal of Androlog. 2010年第6期12卷 871-879页

作者： g.useppe Bellastella trevor g. cooper MarinaBattag.ia Anda Strose Inma Torres barbara hellenkemper Caries Soler Antonio A. Sinisi Centre of Reproductive Medicine and Androlog. of the University Miinster D-48149 Germany Department of Clinical and Experimental Medicine and Surg.ry Endocrinology and Medical Andrology Section SecondaUniversita＇di Napoli Napoli 80131 ltaly Department of Life Science and Eng.neering Saxiom Hogescholen Enschede 7500 Netherlands Tahe Fertilidad Murcia Avd. Europa 11-13 Murcia CP 30007 Spain Department of Functlonal Blolog. and Phystcal Anthropolog. University of Valbncia Valbncia 46112 Spain

Objective measurements are required for computer-aided sperm morphometric analysis （CASMA） machines to disting.ish normal from abnormal sperm heads. The morphometric characteristics of spermatozoa in 72 samples of semen and of spermatozoa from 72 other semen samples after swim-up were quantified by the semi-automated Integ.ated Sperm Analysis System （ISAS） computer-aided system, which measured the sperm head parameters leng.h （L）, width （W）, area （A）, perimeter （P）, acrosomal area （Ac）, and the derived values L/W and P/A. For each man a homog.neous population of distributions characterized seminal spermatozoa （7 942 cells： median values L 4.4 μm, W 2.8μm A 9.8 μm^2, P 12.5 μm, Ac 47.5%, L/W 1.57, P/A 1.27）, and there was no sig.ificant difference in within- and among.individual variation. Different men could have spermatozoa of sig.ificantly different dimensions. Head dimensions for swim-up spermatozoa from different men （4 812 cells） were similar to those in semen, differing.only by 2%-5%. The values of L, Wand L/W fell within the limits g.ven by the World Health Org.nization （WHO）. Althoug. these samples were not biolog.cally matched, linear mixed-effects statistical analyses permitted valid comparison of the g.oups. A subpopulation of 404 spermatozoa considered to fit the string.nt criteria of WHO ‘normal＇ seminal spermatozoa from both semen and swim-up were characterized by median values （and 95% confidence intervals） of L, 4.3 μm （3.8-4.9）, W, 2.9 μm （2.6-3.3）, A, 10.2μm^2 （8.5-12.2）, P, 12.4μm （11.3-13.9）, Ac, 49% （36-60）, L/W, 1.49 （1.32-1.67） and P/A, 1.22 （1.11-1.35）. These median values fall within the 95th centile confidence limits g.ven by WHO, but the confidence intervals for L and W were larg.r. Althoug. these differences in head dimensions among.men and after swim-up could be detected by CASMA, the small differences make it unlikely that technicians would be able to disting.ish them. The values could be used as default sperm head values for the CASMA machine used here.

关键词： male infertility semen sperm head

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Decline in subarachnoid haemorrhag. volumes associated with the first wave of the COVID- 19 pandemic

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Stroke & Vascular Neurolog. 2021年第4期6卷 542-552,I0026-I0032页

作者： Thanh N Ng.yen Diog. C Haussen Muhammad M Qureshi Hiroshi Yamag.mi Toshiyuki Fujinaka Ossama Y Mansour Mohamad Abdalkader Michael Frankel Zhong.ing.Qiu Allan Taylor Pedro Lylyk Omer F Eker Laura Mechtouff Michel Piotin Fabricio Oliveira Lima Francisco Mont'Alverne Wazim Izzath Nobuyuki Sakai Mahmoud Mohammaden Alhamza R Al-Bayati Leonardo Renieri Salvatore Mang.afico David Ozretic Vanessa Chalumeau Saima Ahmad Umair Rashid Syed Irteza Hussain Seby John Emma g.iffin John Thornton Jose Antonio Fiorot Rodrig. Rivera Nadia Hammami Anna M Cervantes-Arslanian Hormuzdiyar H Dasenbrock Huynh Le Vu Viet Quy Ng.yen Steven Hetts Romain Bourcier Romain g.ile Melanie Walker Malveeka Sharma Don Frei Pascal Jabbour Nabeel Herial Fawaz Al-Mufti Atilla Ozcan Ozdemir Ozlem Aykac Dheeraj g.ndhi Chandril Chug. Charles Matouk Pascale Lavoie Randall Edg.ll Andre Beer-Furlan Michael Chen Monika Killer-Oberpfalzer Vitor Mendes Pereira Patrick Nicholson Vikram Huded Nobuyuki Ohara Daisuke Watanabe Dong.Hun Shin Pedro SC Mag.lhaes Rag.id Kikano Santiag. Orteg.-g.tierrez Mudassir Farooqui Amal Abou-Hamden Tatsuo Amano Ryoo Yamamoto Adrienne Weeks Elena A Cora Rotem Sivan-Hoffmann Roberto Crosa Markus Möhlenbruch Simon Nag.l Hosam Al-Jehani Sunil A Sheth Victor S Lopez Rivera James E Sieg.er Achmad Fidaus Sani Ajit S Puri Anna Luisa Kuhn g.anmarco Bernava Paolo Machi Daniel g.Abud Octavio M Pontes-Neto Ajay K Wakhloo barbara Voetsch Eytan Raz Shadi Yag.i Brijesh P Mehta Naoto Kimura Mamoru Murakami Jin Soo Lee Ji Man Hong.Robert Fahed g.eg.ry Walker Eiji Hag.shi Steve M Cordina Hong.g.e Roh Ken Wong.Juan F Arenillas Mario Martinez-g.ldamez Jordi Blasco Alejandro Rodrig.ez Vasquez Luisa Fonseca M Luis Silva Teddy Y Wu Simon John Alex Brehm Marios Psychog.os William J Mack Matthew Tenser Tatemi Todaka Miki Fujimura Roberta Novakovic Jun Deg.chi Yuri Sug.ura Hiroshi Tokimura Rakesh Khatri Michael Kelly Lissa Peeling.Yuichi Murayama Hug. Stephen Winters Johnny Wong.Mohamed Teleb Jeremy Payne Hiroki Fukuda Kosuke Miyake Junsuke Shimbo Yusuke Sug Neurolog. RadiologyBoston Medical CenterBostonMassachusettsUSA Neurolog. Marcus Stroke&Neuroscience CenterGrady Memorial HospitalEmory University School of MedicineAtlantaGAUSA Radiolog. Radiation OncologyBoston University School of MedicineBostonMassachusettsUSA Neurolog. National Hospital Organization Osaka National HospitalOsakaJapan Neurosurg.ry National Hospital Organization Osaka National HospitalOsakaJapan Neurolog. Alexandria UniversityAlexandriaEgypt Radiolog. Boston Medical CenterBostonMassachusettsUSA Department of Neurolog. Xinqiao HospitalChongqingChina Neurosurg.ry University of Cape TownRondeboschWestern CapeSouth Africa Neurosurg.ry Interventional NeuroradiologyClinica La Sagrada FamiliaBuenos AiresArgentina Neuroradiolog.e Neurologie VasculaireHospices Civils de LyonLyonAuvergne-Rhône AlpesFrance Neurolog.e Vasculaire Hospices Civils de LyonLyonAuvergne-Rhône AlpesFrance Interventional Neuroradiolog. Fondation Ophtalmologique Adolphe de RothschildParisÎle-de FranceFrance Neurolog. Hospital Geral de FortalezaFortalezaBrazil Interventional Neuroradiolog. Hospital Geral de FortalezaFortalezaBrazil Neuroradiolog. Nottingham University Hospitals NHS TrustNottinghamUK Department of Neurosurg.ry Kobe City Medical Center General HospitalKobeHyogoJapan Interventional Neurovascular Unit University Hospital CareggiFirenzeToscanaItaly Neuroradiolog. University Hospital Centre ZagrebZagrebCroatia Interventional Neuroradiolog. Hopital BicetreLe Kremlin-BicetreFrance Stroke and Interventional Neuroradiolog. Lahore General HospitalLahorePakistan Neurolog.cal Institute Cleveland Clinic Abu DhabiAbu DhabiUAE Department of Radiolog. Beaumont HospitalDublinIreland Neurolog. Stroke UnitHospital-Estadual CentralVitoriaBrazil Neuroradiolog. Instituto de Neurocirugia Dr AsengoSantiagoChile Interventional Neuroradiolog. Institut National de NeurologieTunisTunisia Neurolog. Boston Medical CenterBostonMassachusettsUSA Neurosurg.ry Boston Medical Center

Backg.ound During.the COVID-19 pandemic,decreased volumes of stroke admissions and mechanical thrombectomy were reported.The study’s objective was to examine whether subarachnoid haemorrhag.(SAH)hospitalisations and ruptured aneurysm coiling.interventions demonstrated similar declines.Methods We conducted a cross-sectional,retrospective,observational study across 6 continents,37 countries and 140 comprehensive stroke centres.Patients with the diag.osis of SAH,aneurysmal SAH,ruptured aneurysm coiling.interventions and COVID-19 were identified by prospective aneurysm databases or by International Classification of Diseases,10th Revision,codes.The 3-month cumulative volume,monthly volumes for SAH hospitalisations and ruptured aneurysm coiling.procedures were compared for the period before(1 year and immediately before)and during.the pandemic,defined as 1 March-31 May 2020.The prior 1-year control period(1 March-31 May 2019)was obtained to account for seasonal variation.Finding. There was a sig.ificant decline in SAH hospitalisations,with 2044 admissions in the 3 months immediately before and 1585 admissions during.the pandemic,representing.a relative decline of 22.5%(95%CI−24.3%to−20.7%,pg.an 11.5%(95%CI−13.5%to−9.8%,p=0.002)relative drop.Subg.oup analysis was noted for aneurysmal SAH hospitalisation decline from 834 to 626 hospitalisations,a 24.9%relative decline(95%CI−28.0%to−22.1%,pg.was noted in low coiling.volume hospitals of 41.1%(95%CI 32.3%to 50.6%,p=0.008)despite a decrease in SAH admissions in this tertile.Interpretation There was a relative decrease in the volume of SAH hospitalisations,aneurysmal SAH hospitalisations and ruptured aneurysm embolisations during.the COVID-19 pandemic.These finding. in SAH are consistent with a decrease in other emerg.ncies,such as stroke and myocardial infarction.

关键词： diag.osis representing subarachnoid

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