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Leukemogenic Fusion Gene (p190 BCR-ABL) Transduction into Hematopoietic Stem/Progenitor Cells in the Common Marmoset

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Open Journal of Blood Diseases 2012年第1期2卷 1-10页

作者： Yan Dong Seiichiro Kobayashi Yamin Tian Manabu Ozawa Takafumi Hiramoto Kiyoko Izawa Yuansong Bai Yasushi Soda Erika Sasaki toshio itoh Yoshiro Maru Satoshi Takahashi Kaoru Uchimaru Naoki Oyaizu Arinobu Tojo Chieko Kai Kenzaburo Tani Central Institute for Experimental Animals Kawasaki Japan Department of Laboratory Medicine Research Hospital Institute of Medical Science the University of Tokyo Tokyo Japan Department of Molecular Genetics Medical Institute of Bioregulation Kyushu University Fukuoka Japan Department of Pharmacology Tokyo Woman’s Medical University Japan Division of Molecular Therapy Institute of Medical Science the University of Tokyo Tokyo Japan Laboratory Animal Research Center Institute of Medical Science the University of Tokyo Tokyo Japan.

Patients with Philadelphia chromosome (p190 BCR-ABL fusion gene)-positive acute lymphoblastic leukemia have a poor prognosis despite intensive therapeutic intervention.In this study, we attempted to develop a leukemia nonhuman primate model that mimics various human systems. Hematopoietic stem/progenitor cells in the common marmoset were transduced with a lentiviral vector containing the p190 BCR-ABL fusion gene by ex vivo transduction or in vivo direct bone marrow injection. In the latter model, BCR-ABL gene expression was maintained for more than one and a half years. One marmoset unexpectedly developed myelofibrosis-like disease. However, none of the marmosets have developed leukemia to date. In conclusion, we successfully achieved sustained p190 BCR-ABL gene expression in vivo. However, a genetic mutation in addition to p190 BCR-ABL may be required for the malignant transformation of hematopoietic stem/progenitor cells in the common marmoset during the short observation period. This novel in vivo approach will help develop a marmoset leukemia model in the future.

关键词： Leukemia Lentiviral Vector Myelofibrosis Common Marmoset

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Near-infrared imaging survey of faint companions around young dwarfs in the Pleiades cluster

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Research in Astronomy and Astrophysics 2011年第3期11卷 335-344页

作者： Yoichi itoh Yumiko Oasa Hitoshi Funayama Masahiko Hayashi Misato Fukagawa toshio Hashiguchi Thayne Currie Graduate School of Science Kobe University1-1 RokkodaiNada-kuKobe 657-8501Japan Faculty of Education Saitama University255 Shimo-OkuboSakura-kuSaitamaSaitama 338-8570Japan Subaru Telescope 650 North A'ohoku PlaceHiloHawaii 96720USA School of Mathematical and Physical Science Graduate University for Advanced Studies(Sokendai)2-21-1 OsawaMitakaTokyo 181-8588Japan Graduate School of Science Osaka UniversityMachikaneyamaToyonakaOsaka 560-0043Japan NASA-Goddard Space Flight Center

We conducted a near-infrared imaging survey of 11 young dwarfs in the Pleiades cluster using the Subaru Telescope and the near-infrared coronagraph imager. We found ten faint point sources,with magnitudes as faint as 20 mag in the K-band,with around seven dwarfs.Comparison with the Spitzer archive images revealed that a pair of the faint sources around V 1171 Tau is very red in infrared wavelengths,which indicates very low-mass young stellar objects.However,the results of our follow-up proper motion measurements implied that the central star and the faint sources do not share common proper motions,suggesting that they are not physically associated.

关键词： planetary systems：formation—techniques：high angular resolution

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Endoscopic enucleation of gastrointestinal stromal tumors of the stomach: Report of five cases

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World Journal of Gastroenterology 2008年第16期14卷 2609-2611页

作者： toshio Katoh Yoshiyuki itoh Tomomi Mohri Hiroshi Suzuki Division of Surgery Toyama Hospital

Gastrointestinal stromal tumor (GIST) of the stomach was treated by endoscopic enucleation in five patients. They were three men and two woman, aged 36-56 years. Tumors located in the cardia were completely enucleated endoscopically without any serious complication. The largest diameter of removed tumors ranged from 1.2 to 2.5 cm. Histopathological diagnosis was GIST with low risk of malignancy (mitotic index < 5/50 high power field) in all cases. The patients were disease-free for 10.5-42.2 mo after endoscopic enucleation.

关键词：胃癌病例症状治疗方法

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Cytosolic chaperonin CCT possesses GTPase activity

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American Journal of Molecular Biology 2011年第3期1卷 123-130页

作者： Susumu Noguchi Kazuyoshi Toyoshima Soh Yamamoto toshio Miyazaki Michiro Otaka Sumio Watanabe Katsunori Imai Haruki Senoo Ryoji Kobayashi Mitsutoshi Jikei Yasushi Kawata Hiroshi Kubota Hideaki itoh 不详

Cytosolic chaperonin CCT (also known as TRiC) is a hetero-oligomeric cage-like molecular chaperone that assists in protein folding by ATPase cycle-dependent conformational changes. However, role of the nucleo-tide binding and hydrolysis in CCT-assisted protein folding is still poorly understood. We purified CCT by using ATP-Sepharose and other columns, and found that CCT possesses ability to hydrolyze GTP, with an activity level very similar to the ATPase activity. CCT was more resistant to proteinase K treatment in the presence of GTP or ATP. These results suggest that the GTPase activity of CCT may play a role in chaperone-assisted protein folding.

关键词： Chaperonin Molecular Chaperone Protein Folding Gtp

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