检索结果-南通市图书馆

Protective effect of estradiol on hepatocytic oxidative damage

World Journal of Gastroenterology 2002年第2期8卷 363-366页

作者： Ichiro Shimizu toshihiro omoya Susumu Ito Second Department of Internal Medicine School of MedicineUniversity of TokushimaTokushima770-8503Japan

AIM: To examine the protective effect of estradiol on the cultured hepatocytes under oxidative stress. METHODS: Hepatocytes of rat were isolated by using perfusion method, and oxidative stress was induced by a serum-free medium and FeNTA. MDA level was determined with TBA method. Cell damage was assessed by LDH assay. Apoptosis of hepatocytes was assessed with cytoflowmetric analysis. Expression of Bcl-xl in cultured hepatocytes was detected by Western blot. The radical-scavenging activity of estradiol was valued by its ability to scavenge the stable free radical of DDPH. RESULTS: Oxidative stress increased LDH from 168 +/- 25 x 10(-6)***(-1) to 780 +/- 62 x 10(-6)***(-1) and MDA(from 0.28 +/- 0.07 x 10(-6)***(-1) to 1.35 +/- 0.12 x 10(-6)***(-1)) levels in cultured hepatocyte, and estradiol inhibited both LDH and MDA production in a dose dependent manner. In the presence of estradiol 10(-6)mol.L(-1), 10( -7 )mol.L(-1) and 10(-8)mol.L(-1),the LDH levels are 410 +/- 53 x 10(-6)***(-1) (P<0.01 vs oxidative group), 530 +/- 37 X 10(-6)***(-1 ) (P<0.01 vs oxidative group), 687+/-42 x 10(-6)***(-1) (P<0.05 vs oxidative group) respectively, and the MDA level are 0.71+/-0.12 x 10(-6)***(-1) (P<0.01 vs oxidative group),0.97+/-0.11 x 10(-6)***(-1 )(P<0.01 vs oxidative group) and 1.27+/-0.19 x 10(-6)***(-1) respectively. Estradiol suppressed apoptosis of hepatocytes induced by oxidative stress, administration of estradiol(10(-6)mol/L)decreased the apoptotic rate of hepatocytes under oxidative stress from 18.6 +/- 1.2% to 6.5 +/-2.5%, P<0.01. Bcl-xl expression was related to the degree of liver cell damage due to oxidative stress, and estradiol showed a protective action. CONCLUSION: Estradiol protects hepatocytes from oxidative damage by means of its antioxidant activity.

关键词： Oxidative Stress Animals Apoptosis Cells, Cultured Estradiol Female Flow Cytometry Hepatocytes L-Lactate Dehydrogenase Lipid Peroxidation Male Proto-Oncogene Proteins c-bcl-2 Rats Rats, Wistar Thiobarbituric Acid Reactive Substances bcl-X Protein

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：