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Activation of protein kinase A alters subnuclear distribution pattern of human steroidogenic factor 1 in living cells

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Chinese Medical Journal 2004年第7期117卷 1017-1022页

作者：刘伟范吴强 toshihiko yanase Masayuki Saitoh 吴茵 Department of Medicine and Endocrinology Department of Medicine and Bioregulatory Science Department of Geriatric Medicine

Background The aim of this study was to identify the subnuclear distribution pattern of human orphan nuclear receptor steroidogenic factor 1 (SF-1) in living cells with and without the activation of protein kinase A (PKA) signal pathway, and thus try to explain the unknown mechanism by which PKA potentiates SF-1 transactivation. Methods Full-length cDNAs of wild type and a naturally occurring mutant (G35E) human SF-1 were cloned and fused with green fluorescent protein (GFP). Subcellular distribution pattern of human SF-1 in living cells, whose PKA signaling was either activated or not, was studied by laser confocal microscopy after the validity of the gene sequence was *** The transactivation ability of the GFP-SF-1 chimeric protein was highly conserved. Wild type human SF-1 diffused homogeneously within the nuclei of cells when PKA was not active, and converged to clear foci when PKA was activated. Mutant SF-1 diffused within the nuclei even in the presence of PKA activation, surprisingly aggregating as fluorescent dots inside the nucleoli, a phenomenon not altered by *** Activation of PKA causes wild type, but not mutant SF-1 to alter its subnuclear distribution pattern to a transactivationally active form (foci formation). This finding may throw new light on the mechanism by which PKA activates the orphan nuclear receptor.

关键词： steroidogenic factor 1· protein kinase A· green fluorescent protein

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Androgen and metabolic syndrome

Androgen and metabolic syndrome

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中华医学会第十次全国内分泌学学术会议

作者： toshihiko yanase Department of Endocrinology and Diabetes Mellitus School of MedicineFukuoka UniversityNanakuma 7-45-1Jonan-kuFukuoka814-0180 Japan

Androgen is an important factor for determining body composition in *** obesity is inversely correlated with serum androgen levels in *** receptor(AR) null male mice revealed late-onset visceral obesity,accompanying energy balance abnormality. We identified a novel synthetic steroid,S42 as a promising candidate of a selective androgen receptor modulator(SARM).Results of the whole-cell binding assay using COS-7 cells exogenously expressing various steroid receptors indicated that S42 specifically

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A Micromixer Using the Taylor-Dean Flow: Effects of Aspect Ratio and Inflow Condition on the Mixing

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Open Journal of Fluid Dynamics 2015年第3期5卷 256-264页

作者： Yasutaka Hayamizu toshihiko Kawabe Shinichiro yanase Takeshi Gonda Shinichi Morita Shigeru Ohtsuka Kyoji Yamamoto Department of Mechanical Engineering Yonago National College of Technology Tottori Japan Technical Division Tsurumi Manufacturing Co. Ltd. Tottori Japan Graduate School of Natural Science and Technology Okayama University Okayama Japan

Chaotic mixing in three different types of curved-rectangular channels flow has been studied experimentally and numerically. Two walls of the channel (inner and top walls) rotate around the center of curvature and a pressure gradient are imposed in the direction toward the exit of the channel. This flow is a kind of Taylor-Dean flow. There are two parameters dominating the flow, the Dean number De (∝ the pressure gradient or the Reynolds number) and the Taylor number Tr (∝ the angular velocity of the wall rotation). In this paper, we analyze the physical mechanism of chaotic mixing in the Taylor-Dean flow by comparing experimental results and numerical ones. We produced three micromixer models of the curved channel, several centimeters long, with rectangular cross-section of a few millimeters side. The secondary flow is measured using laser induced fluorescence (LIF) method to examine secondary flow characteristics. Also we performed three-dimensional numerical simulations with the open source CFD solver, OpenFOAM, for the same configuration as the experimental system to study the mechanism of chaotic mixing. It is found that good mixing performance is obtained in the case of De ≤ 0.1 Tr, and it becomes more remarkable when the aspect ratio tends to large. And it is found that the mixing efficiency changes according to the aspect ratio and inflow condition.

关键词： Component Taylor-Dean Flow Chaotic Mixing Secondary Flow LIF CFD

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A Micromixer Using the Taylor-Dean Flow: Effect of Inflow Conditions on the Mixing

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Open Journal of Fluid Dynamics 2014年第5期4卷 463-471页

作者： toshihiko Kawabe Yasutaka Hayamizu Shinichiro yanase Takeshi Gonda Shinichi Morita Shigeru Ohtsuka Kyoji Yamamoto Technical Division Tsurumi Manufacturing Co. Ltd. Tottori Japan Department of Mechanical Engineering Yonago National College of Technology Tottori Japan Graduate School of Natural Science and Technology Okayama University Okayama Japan

Chaotic mixing in a curved-square channel flow is studied experimentally and numerically. Two walls of the channel (inner and top walls) rotate around the center of curvature and a pressure gradient is imposed in the direction toward the exit of the channel. This flow is a kind of Taylor-Dean flows. There are two parameters dominating the flow, the Dean number De (∝ the pressure gradient or the Reynolds number) and the Taylor number Tr (∝ the angular velocity of the wall rotation). In the present paper, we analyze the physical mechanism of chaotic mixing in the Taylor-Dean flow by comparing experimental and numerical results. We produced a micromixer model of the curved channel several centimeters long with square cross section of a few millimeters side. The secondary flow was measured using laser induced fluorescence (LIF) method to examine secondary flow characteristics. We also performed three-dimensional numerical simulations for the exactly same configuration as the experimental system to study the mechanism of chaotic mixing. It is found that good mixing performance is achieved for the case of De ≤ 0.1Tr, and that mixing efficiency changes according to the difference in inflow conditions. The flow is studied both experimentally and numerically, and both results agree with each other very well.

关键词： Taylor-Dean Flow Chaotic Mixing Secondary Flow LIF CFD

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