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‘京白梨’优良单株组织培养高效快繁体系构建

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广西林业科学 2019年第2期48卷 173-178页

作者：刘春高同雨苏本营李锦锦李晶武雅娟王巍沈应柏北京市门头沟区国家生态修复科技综合示范基地北京102300 淮北师范大学安徽淮北235000 北京林业大学生物科学与技术学院北京100083

试验以‘京白梨’优良单株‘孟3’为试验材料,旨在通过外植体筛选、消毒方式优化、增殖和生根培养基筛选,建立‘京白梨’高效快繁体系,为脱毒苗快速培育提供科学支撑。结果表明:选用老枝条为外植体进行接种后污染率达到93.3%,而采用嫩... 详细信息

试验以‘京白梨’优良单株‘孟3’为试验材料,旨在通过外植体筛选、消毒方式优化、增殖和生根培养基筛选,建立‘京白梨’高效快繁体系,为脱毒苗快速培育提供科学支撑。结果表明:选用老枝条为外植体进行接种后污染率达到93.3%,而采用嫩梢污染率为0,因此在外植体的选择上应尽量采用‘京白梨’植株幼嫩组织,如花芽或刚萌发的嫩梢。采用2%的次氯酸钠与0.1%的升汞消毒,污染率分别为33%、18%,虽然升汞比次氯酸钠处理效果好,但考虑到安全和环境保护,建议使用次氯酸钠进行外植体消毒。1/2MS培养基与1.5mg/L赤霉素浓度对‘京白梨’花芽愈伤组织诱导较好。对‘京白梨’优良单株的组培体系建立过程中发现,采用3mg/L6-BA+0.5mg/LNAA+1.5mg/LGA3对‘京白梨’‘孟3’外植体的愈伤组织形成、不定芽个数的形成、增殖率、芽长度、叶片生长情况有良好的促进作用,增殖率为94%。生根培养试验结果表明:在S3、S5、S7、S9培养基中,15d观察后发现‘京白梨’组培苗的基部有根原基形成,但在此期间未诱导形成根,45d后观察发现,S10、S11培养基中的‘京白梨’组培苗成功诱导生根。以植株幼嫩组织作为外植体、2%的次氯酸钠作为消毒剂、采用3mg/L6-BA+0.5mg/LNAA+1.5mg/LGA3作为增殖培养基、S10和S11作为生根培养基,可有效提高‘京白梨’优良单株组培快繁育苗效率。

关键词：京白梨优良单株组织培养高效快速繁育

北京市现代化都市型农业经营活动碳足迹研究──以门头沟区京白梨果园为例

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中国农学通报 2016年第3期32卷 128-134页

作者：杨自立付军利高同雨柴迪迪武雅娟李志雄王林峰北京市门头沟区科学技术委员会北京102300 北京市门头沟区国家生态修复科技综合示范基地北京102300 山东省农垦科技发展中心济南250013

本研究旨在通过科学评价京白梨果园生产经营过程所产生的碳排放,为构建农业低碳管理技术体系,实现北京市现代化都市型农业经营活动的节能减排奠定基础。笔者基于对10年生产经营活动数据的调查,通过运用农业碳足迹的基本理论和研究方法,... 详细信息

本研究旨在通过科学评价京白梨果园生产经营过程所产生的碳排放,为构建农业低碳管理技术体系,实现北京市现代化都市型农业经营活动的节能减排奠定基础。笔者基于对10年生产经营活动数据的调查,通过运用农业碳足迹的基本理论和研究方法,测算了门头沟区6个主要京白梨果园的碳足迹。结果表明：6个京白梨果园年均碳足迹在118.71-513.23 kg Ce/（hm^2·a）之间,生产1 kg京白梨鲜果的碳成本在0.12-0.27 kg Ce/kg之间。各个果园碳足迹的组成中,灌溉电能消耗量占总量的16.97%-83.20%,化肥占0.00%-63.12%,柴油占8.68%-50.18%,农药占2.09%-7.39%。另外,灌溉电能消耗量或N肥施用量与各个果园的年总碳足迹存在正相关性。因此,制定并实施科学合理的京白梨精准灌溉施肥田间管理制度,以及因地制宜,开展雨洪利用工程的建设,减少果园灌溉对地下水的依赖,是降低门头沟京白梨果园经营管理过程中碳足迹的有效途径。

关键词：京白梨果园碳足迹碳成本

Smart nanocarriers as therapeutic platforms for bladder cancer

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Nano Research 2022年第3期15卷 2157-2176页

作者： tongyu Tong Yupeng Guan Yuanji gao Chengyuan Xing Shiqiang Zhang Donggen Jiang Xiangwei Yang Yang Kang Jun Pang Department of Urology Kidney and Urology Centerthe Seventh Affiliated HospitalSun Yat-sen UniversityShenzhen 518107China Scientific Research Center the Seventh Affiliated HospitalSun Yat-sen UniversityShenzhen 518107China College of Chemistry and Materials Science Sichuan Normal UniversityChengdu 610068China

Although patients benefit from surgical transurethral resection of bladder cancers,some niduses are missed or incompletely resected,and small malignant lesions may *** chemotherapy and immunotherapy are universally accepted as adjuvant treatments after surgery to avoid recurrence and ***,these treatments still have limitations,including an insufficient retention period,inefficient permeability of chemotherapeutic agents,and dilution of the agents by ***-based smart therapeutic platforms can be tailored to be responsive to internal or external stimuli to ameliorate this *** unparalleled capability empowers the precise aggregation of stimulus–responsive nanocarriers in the target regions,namely,the tumors,and subsequent release of the anticancer *** review summarizes the current nanostructure-based therapeutic platforms,especially stimulus–responsive nanocarriers,and highlights their benefits and limitations in bladder cancer *** innovations in nanotechnology have undoubtedly arrived at a new height and have become useful for practical *** will positively promote the development of anticancer agents not only for bladder cancer but also for other solid tumors.

关键词： nanocarriers stimulus-responsive therapeutic platform drug delivery bladder cancer

北京妙峰山玫瑰花渣中黄酮类成分的UPLC/Q-TOF-MS分析

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中国现代中药 2017年第11期19卷 1550-1554页

作者：王方方康莹张权齐梦蝶刘永刚高同雨武雅娟刘勇北京中医药大学北京100102 北京市门头沟区国家生态修复科技综合示范基地北京102300

目的:采用UPLC/Q-TOF-MS技术对玫瑰花渣中的黄酮类成分进行定性分析。方法:采用Waters BEH C18色谱柱(100 mm×2.1 mm,1.7μm),柱温:40℃,洗脱系统为乙腈-0.1%甲酸水梯度洗脱;采用ESI离子源并在负离子模式下进行采集数据。结果:通过UPL... 详细信息

目的:采用UPLC/Q-TOF-MS技术对玫瑰花渣中的黄酮类成分进行定性分析。方法:采用Waters BEH C18色谱柱(100 mm×2.1 mm,1.7μm),柱温:40℃,洗脱系统为乙腈-0.1%甲酸水梯度洗脱;采用ESI离子源并在负离子模式下进行采集数据。结果:通过UPLC的快速分离,根据质谱数据并结合文献分析推测,鉴定了11种黄酮类化合物。结论:采用UPLC/Q-TOF-MS技术,可有效地分析玫瑰花渣中黄酮类成分,为玫瑰花渣的回收利用提供了数据支持。

关键词： UPLC/Q-TOF-MS 玫瑰花渣黄酮类化合物北京妙峰山

Influence of Different Connection Types on Mechanical Behavior of Girder Trusses

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Journal of Bioresources and Bioproducts 2019年第2期4卷 89-98页

作者： Zeli QUE tongyu HOU Yifan gao Qicheng TENG Qiuyun CHEN Changju WANG Cheng CHANG Department of Wooden Structure Construction Engineering Nanjing Forestry UniversityNanjing 210037China

The static test of two single light wood trusses and two girder trusses were carried out to explore theperformance enhancement effect of girder trusses and the influence of different connection types on the mechanicalbehavior, which was studied through the comparison of the test results of four trusses. It is found that the girder trussescan show better load-carrying capacity and anti-deforming ability compared with which simply increases the cross-sectionsize of the members. Because of the advantage of the connection materials and the non-fastening connection mechanism,the wood pin-connected truss has more ultimate bearing capacity which is 2.6 times of single truss, and better deformationresistance which is only 66.2% of the nailed one’s creep value.

关键词： girder truss multiple combination connection node design static load test wood pin

Thiourea-assisted coating of dispersed copper electrocatalysts on Si photocathodes for solar hydrogen production

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Journal of Energy Chemistry 2020年第1期29卷 75-80,I0004页

作者： Pin Wang Ziyu Yin Linfeng gao Hui Li tongyu Zhang Qing Shen Jun Lv Yingfang Yao Wenjun Luo Zhigang Zou Ecomaterials and Renewable Energy Research Center(ERERC) Department of PhysicsNanjing UniversityNanjing 210093JiangsuChina Jiangsu Key Laboratory for Nano Technology Nanjing National Laboratory of Solid State MicrostructuresCollege of Engineering and Applied SciencesNanjing UniversityNanjing 2J0093JiangsuChina Key Laboratory of Flexible Electronics(KLOFE)&Institute of Advanced Materials(IAM) Jiangsu National Synergetic Innovation Center for Advanced Materials(SICAM)Nanjing Tech UniversityNanjing 211816JiangsuChina Department of Engineering Science The University of Electro Communications1-5-1 ChofugaokaChofiiTokyo 182-8585Japan Electronic Information Engineering College of Sanjiang University Nanjing 210012JiangsuChina Jiangsu Key Laboratory for Nano Technology Nanjing National Laboratory of Solid State Microstructures.College of Engineering and Applied SciencesNanjing UniversityNanjing 210093JiangsuChina

Photoelectrochemical water splitting can convert solar energy into clean hydrogen energy for *** is desirable to explore non-precious electrocatalysts for practical applications of a photoelectrode in a large ***,we developed a facile spin-coating and in-situ photoelectrochemical reduction method to prepare a dispersed Cu electrocatalyst on a Si photocathode,which improves the performance *** find that thiourea in the precursor solution for spin-coating plays an important role in obtaining dispersed Cu particles on the surface of a Si *** thiourea in the precursor,the Cu/Si photocathode shows higher performance than the one without ***,the Cu/Si photocathode also indicates good stability after 16 h illumination.

关键词： Thiourea Hydrogen evolution reaction In-situ photoelectrochemical reduction Cu electrocatalyst

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基于QAR数据的飞行控制系统故障研究综述

中国安全科学学报 2024年第4期34卷 1-9页

作者：王岩韬高艺时统宇中国民航大学国家空管运行安全技术重点实验室天津300300

为系统梳理国内外对民用飞机飞行控制系统故障分析的研究历程和现状,针对基于快速存取记录器(QAR)数据分析的飞行控制系统典型故障类型,首先,总结QAR数据预处理、特征提取等使用过程;然后,根据故障分析可达到的性能指标,提出4个故障研... 详细信息

为系统梳理国内外对民用飞机飞行控制系统故障分析的研究历程和现状,针对基于快速存取记录器(QAR)数据分析的飞行控制系统典型故障类型,首先,总结QAR数据预处理、特征提取等使用过程;然后,根据故障分析可达到的性能指标,提出4个故障研究阶段,分别为故障监测、故障识别、故障诊断和故障预测;最后,综合国内外研究进度与深度,得出飞行控制系统典型故障类型,包括方向舵液压泄漏、升降舵指示不一致、襟翼动作耗时等,建模常用QAR数据项包括飞机主舵面位置、飞行姿态、飞机性能、左右襟翼角度、襟翼位置等,计算方法包括物理模型、多变量统计、逻辑推理、机器学习等。结果表明:系统分析方向舵、升降舵、襟翼等子系统最新研究进展,发现在故障类型、参数选择和计算方法的改进等方面取得了一定的成果,故障研究阶段基本处于故障诊断或非实时预测水平,但仍需加强面向安全保障与实际维修方面的需求,以实现故障实时预测技术。

关键词：快速存取记录器(QAR) 飞行控制系统故障监测故障识别故障诊断故障预测

A Discussion on Hygiene Supervising and Control Over Ocean—going Ships Provision

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旅行医学科学 1997年第2期3卷 95-96页

作者： gao Hongxi Wang tongyu Li Juncheng Qinhuangdao Health and Quarantine Bureau, P. R. China

In order to keep crew healthy during their journey and preventing diseases which cause by eating unqualified food. An investigation on sanitary condition of provision over ships have implemented from January to October of 1995. 109 out of 951 units provision were unqualfled (11.48%). Over time and improper storage and unqualified sanitary environment of store rooms were the main reasons for this case. The authors presented the discussion on supervision and control over ships’ provision in this article.

关键词： ships provision supervision and control travel health

Notoginsenoside R1 Protects against Diabetic Nephropathy through TXNIP-NLRP3 Signaling Pathway

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Clinical Complementary Medicine and Pharmacology 2023年第4期3卷 34-48页

作者： Chunting Zhang Renyikun Yuan Siyuan Li Guodong Huang Kaili Sun Jiaping Pan Qiuxia Liu Xiang gao Zhijing Wang tongyu Li Shilong Lu Jianzhen Lv Liting Huang Hongwei gao College of Pharmacy Guangxi University of Chinese MedicineNanning 530200GuangxiChina Guangxi Engineering Technology Research Center of Advantage Chinese Development Nanning 530200GuangxiChina Guangxi International Zhuang Medicine Hospital Nanning 530200GuangxiChina The First Affiliated Hospital of Guangxi University of Chinese Medicine Nanning 530200GuangxiChina Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine Nanning 530001GuangxiChina

Background:Diabetic nephropathy(DN)is a microvascular complication of diabetes mellitus(DM).DN results from many factors,including changes in glomerular hemodynamics,oxidative stress and inflammation,and in-terstitial fibrosis and tubular *** notoginseng,a commonly used Chinese medicine,has been used in the treatment of kidney *** R1(NGR1),the main compound isolated from ***,has been reported to have a renoprotective role in ***,the therapeutic effect and mechanism of NGR1 in DN remain ***:The present study aimed to investigate the therapeutic effect and mechanism of NGR1 in ***:In this study,mouse podocytes(MPC-5 cells)and db/db mice were used to investigate the effect of NGR1 on DN in vitro and in vivo,*** glucose,renal function,inflammatory factors,and PI3K/AKT-Nrf2-NLRP3 signaling pathway proteins were ***:The study results indicated that NGR1 reversed cell viability induced by high glucose(HG,30 mM).The related mechanism results showed that NGR1 decreased oxidative stress by inhibiting reactive oxygen species(ROS)level and upregulating the expression of Nrf2,NQO1,and HO-1 via TXNIP *** addition,NLRP3 inflammasome and PI3K/AKT were engaged in NGR1-based protection against HG-stimulated *** db/db mice,NGR1 significantly decreased blood glucose,urine protein,urine output,blood urea nitrogen,and other parameters as well as reversed kidney injury by inhibiting oxidative stress and proinflammatory ***:Taken together,this study revealed that NGR1 exerted a significant therapeutic effect on DN both in vitro and in vivo via a mechanism related to the TXNIP-Nrf2 pathway and NLRP3 inflammasome,suggesting that NGR1 is a potential therapeutic option for DN.

关键词： Diabetic nephropathy NGR1 PI3K/AKT NRF2 TXNIP NLRP3 inflammasome