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Spontaneous mutations and mutational responses to penicillin treatment in the bacterial pathogen Streptococcus pneumoniae D39

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Marine Life Science & Technology 2024年第3期6卷 198-211页

作者： Wanyue Jiang tongtong lin Jiao Pan Caitlyn ERivera Clayton Tincher Yaohai Wang Yu Zhang Xiang Gao Yan Wang Ho‑Ching T.Tsui Malcolm E.Winkler Michael Lynch Hongan Long Institute of Evolution and Marine Biodiversity KLMMEOcean University of ChinaQingdao 266003China Laboratory for Marine Biology and Biotechnology Laoshan LaboratoryQingdao 266237China Department of Biology Indiana UniversityBloomingtonIN 47405USA School of Mathematics Science Ocean University of ChinaQingdao 266000China State Key Laboratory of Microbial Technology Microbial Technology InstituteSchool of Life ScienceShandong UniversityQingdao 266237China Biodesign Center for Mechanisms of Evolution Arizona State UniversityTempeAZ 85281USA

Bacteria with functional DNA repair systems are expected to have low mutation rates due to strong natural selection for genomic ***,our study of the wild-type Streptococcus pneumoniae D39,a pathogen responsible for many common diseases,revealed a high spontaneous mutation rate of 0.02 per genome per cell division in mutation-accumulation(MA)*** rate is orders of magnitude higher than that of other non-mutator bacteria and is characterized by a high mutation bias in the A/T *** high mutation rate may have resulted from a reduction in the overall efficiency of selection,conferred by the tiny effective population size in *** line with this,*** D39 also exhibited the lowest DNA mismatch-repair(MMR)efficiency among *** with the antibiotic penicillin did not elevate the mutation rate,as penicillin did not induce DNA damage and *** lacks a stress response *** findings suggested that the MA results are applicable to within-host scenarios and provide insights into pathogen evolution.

关键词： Neutral evolution Mutation spectrum Antibiotics DNA mismatch repair Oxidative damage repair

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大学生公益创新创业育人路径:基于案例分析

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科技创业月刊 2024年第3期37卷 61-65页

作者：郭炎华蓝永胜陈林林林彤彤桂林航天工业学院外语外贸学院桂林航天工业学院国有资产管理处广西桂林541004

基于多重螺旋式公益创业协同创新育人框架模型,利用案例分析法分析创业者在思维激活、模式构建和实施评估方面是如何进行社会认知与创新的。研究发现,《理解当代中国》教材的育人理念有助于大学生认识经济社会存在的问题并萌生社会创新... 详细信息

基于多重螺旋式公益创业协同创新育人框架模型,利用案例分析法分析创业者在思维激活、模式构建和实施评估方面是如何进行社会认知与创新的。研究发现,《理解当代中国》教材的育人理念有助于大学生认识经济社会存在的问题并萌生社会创新创业意识。通过分析该公益创业的协同模式构架、资金筹募和养老公益积分储蓄系统构建,发现其有着公益协同创新、“客制化”管理、蝴蝶效应创新和公益接力棒传承特色等创新点。对社会创新类公益创业朝着融合型、协同化的持续性发展有一定参考作用。

关键词：创业教育公益创业协同创新

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Contribution of the SOS response and the DNA repair systems to norfloxacin induced mutations in ***

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Marine Life Science & Technology 2023年第4期5卷 538-550页

作者： tongtong lin Jiao Pan Colin Gregory Yaohai Wang Clayton Tincher Caitlyn Rivera Michael Lynch Hongan Long Yu Zhang Institute of Evolution and Marine Biodiversity KLMMEOcean University of ChinaQingdao 266003China Laboratory for Marine Biology and Biotechnology Laoshan LaboratoryQingdao 266237China Department of Biology Indiana UniversityBloomington 47405USA Biodesign Center for Mechanisms of Evolution Arizona State UniversityTempe 85281USA School of Mathematics Science Ocean University of ChinaQingdao 266000China

Antibiotic-resistant bacteria severely threaten human *** spontaneous mutations generated by endogenous factors,the resistance might also originate from mutations induced by certain antibiotics,such as the *** antibiotics increase the genome-wide mutation rate by introducing replication errors from the SOS response pathway or decreasing the efficiency of the DNA repair ***,the relative contributions of these molecular mechanisms remain unclear,hindering understanding of the generation of resistant ***,using newly-accumulated mutations of wild-type and SOS-uninducible Escherichia coli strains,as well as those of the strains deficient for the mismatch repair(MMR)and the oxidative damage repair pathways,we find that the SOS response is the major mutagenesis contributor in mutation elevation,responsible for~30–50%of the total base-pair substitution(BPS)mutation-rate elevation upon treatment with sublethal levels of norfloxacin(0~50 ng/mL).We further estimate the significance of the effects on other mutational features of these mechanisms(i.e.,transversions,structural variations,and mutation spectrum)in *** using linear *** SOS response plays a positive role in all three mutational features(mutation rates of BPSs,transversions,structural variations)and affects the mutational *** repair systems significantly reduce the BPS mutation rate and the transversion rate,regardless of whether antibiotics are present,while significantly increasing the structural variation rate in *** results quantitatively disentangle the contributions of the SOS response and DNA repair systems in antibiotic-induced mutagenesis.

关键词： Stress response Antibiotic-induced mutagenesis Mutation spectrum linear model

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An integrative protocol for one‑step PCR amplicon library construction and accurate demultiplexing of pooled sequencing data

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Marine Life Science & Technology 2023年第4期5卷 564-572页

作者： Jiahao Ni Jiao Pan Yaohai Wang Tianhao Chen Xinshi Feng Yichen Li tongtong lin Michael Lynch Hongan Long Weiyi Li Institute of Evolution and Marine Biodiversity KLMMEOcean University of ChinaQingdao 266003China Laboratory for Marine Biology and Biotechnology Laoshan LaboratoryQingdao 266237China Biodesign Center for Mechanisms of Evolution Arizona State UniversityTempeAZ 85281USA Department of Biology Indiana UniversityBloomingtonIN 47401USA Present Address:SLAC National Accelerator Laboratory Stanford UniversityStanford 94305USA

High-throughput sequencing of amplicons has been widely used to precisely and efficiently identify species compositions and analyze community structures,greatly promoting biological studies involving large amounts of complex samples,especially those involving environmental and pathogen-monitoring *** library preparation kits for amplicon sequencing,which generally require multiple steps,including adapter ligation and indexing,are expensive and time-consuming,especially for applications at a large *** overcome these limitations,a“one-step PCR approach”has been previously proposed for constructions of amplicon libraries using long fusion ***,efficient amplifications of target genes and accurate demultiplexing of pooled sequencing data remain to be *** tackle these,we present an integrative protocol for one-step PCR amplicon library construction(OSPALC).High-quality reads have been generated by this approach to reliably identify species compositions of mock bacterial communities and environmental *** this protocol,the amplicon library is constructed through one regular PCR with long primers,and the total cost per DNA/cDNA sample decreases to just 7%of the typical cost via the multi-step PCR *** tested primers and optimized PCR conditions to construct OSPALC libraries for 16S rDNA V4 regions are demonstrated as a case *** to design primers targeting at any genomic regions are also *** principle,OSPALC can be readily applied to construct amplicon libraries of any target genes using DNA or RNA samples,and will facilitate research in numerous fields.

关键词： Amplicon library preparation Lab-made protocol Long-primer PCR Cost efficiency

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Luteolin promotes neuronogenesis in hippocampus of chronic unpredictable mild stress rats and primary hippocampus of fetal rats

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Journal of Traditional Chinese Medicine 2024年第4期44卷 670-679页

作者： LIU tongtong ZHANG Xi YANG Hui lin Xiaoyuan LIU Jian ZHANG Xiuli GUO Dongwei ZHAO Hongqing ZOU Manshu LEI Chang LONG Hongping LUO Yan XIANG Yun GE Jinwen WANG Yuhong MENG Pan Institute of Innovation and Applied Research in Chinese Medicine Hunan University of Chinese MedicineChangsha 410208China the Second People's Hospital of Hunan Province Changsha 410007China The First Affiliated Hospital Hunan University of Chinese MedicineChangsha 410007China Hunan Academy of Chinese Medicine Changsha 410013China

OBJECTIVE:To investigate the effects of luteolin on chronic unpredictable mild stress(CUMS)-induced depressive rats and corticosterone(CORT)-induced depressive primary hippocampal neurons,and to elucidate the mechanism behind the ***:The antidepressant mechanism of luteolin was studied by using CUMS rat model and primary hippocampal neurons in fetal *** vivo,novelty suppressed feeding,open-field and sucrose preference tests as well as Morris water maze were *** content of brain derived neurotrophic factor(BDNF),5-hydroxytryptamine(5-HT),norepinephrine(NE),and dopamine(DA)in serum were detected by enzyme-linked immunosorbent *** mechanisms of luteolin were explored based on neurotrophin and hippocampal neurogenesis,and *** of the septotemporal axis in hippocampus was assayed using the 5-bromo-2-deoxyuridine(BrdU),the expression of BDNF,neurotrophin-3(NT-3),and nerve growth factor(NGF)in hippocampus dentate gyrus region were measured by *** vitro,BDNF,NT-3,tropomyosin receptor kinase B(TrkB),and phosphorylated cyclic adenosine monophosphate responsive element binding protein(p-CREB)were detected through the high content analysis(HCA)to investigate neurotrophin and ***:Induction of CUMS in rats induced depressive symptoms,while luteolin significantly enhanced sucrose consumption,decreased feeding latency,increased locomotor activity,escape latency,distance of target quadrant and regulated the content of depressive-like *** analysis revealed that luteolin increased the abundance of new born neurons that had been labeled with BrdU,BrdU+neuronal nuclear antigen,and BrdU+doublecortin in septo-temporal axis of S2(mid-septal)and T3(mid-temporal).Moreover,expression of BDNF,NT-3,and NGF increased significantly in the septo-temporal axis of S2 and *** showed increased expression of BDNF,NT-3,TrkB and p-CREB in primary hippocampal ***:The results provided direct ev

关键词： fetal rats Survival

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民办高校行政管理人员职业危机防控研究

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科教文汇 2019年第1期 12-14页

作者：林彤彤中山大学新华学院广东广州510520

系统的高校行政工作体系是保证高校教学活动顺利开展的前提,也是高校发展重要的组成部分。民办高校作为教育事业发展的重要增长点和促进教育改革的重要力量,其行政体系具有较强的研究意义。随着我国高等教育全面普及化工作的进一步推动... 详细信息

系统的高校行政工作体系是保证高校教学活动顺利开展的前提,也是高校发展重要的组成部分。民办高校作为教育事业发展的重要增长点和促进教育改革的重要力量,其行政体系具有较强的研究意义。随着我国高等教育全面普及化工作的进一步推动,高校行政管理人员队伍日益壮大。如何打造高质量的高校行政队伍,更好地为教学活动和科研活动提供有力支持,是民办高校亟待思考的问题。本文从民办高校行政管理人员职业危机防控角度出发,分析其现状及存在问题,提出建议和策略,为民办高校行政管理创新及改革提供新的思路。

关键词：高校行政管理人员职业危机防控策略

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抗糖尿病药物的药理研究进展

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基层医学论坛 2011年第4期15卷 167-169页

作者：林彤彤揭阳市人民医院广东揭阳522000

糖尿病（diabotes mellitus,DM）是一组以长期血葡萄糖（简称血糖）水平增高为特征的代谢疾病群,是由遗传因素与环境因素共同作用引起的一种慢性高血糖状态。引起血糖增高的病理生理机制主要是胰岛素分泌缺陷和（或）胰岛素作用缺陷。... 详细信息

糖尿病（diabotes mellitus,DM）是一组以长期血葡萄糖（简称血糖）水平增高为特征的代谢疾病群,是由遗传因素与环境因素共同作用引起的一种慢性高血糖状态。引起血糖增高的病理生理机制主要是胰岛素分泌缺陷和（或）胰岛素作用缺陷。慢性高血糖将导致多种组织,特别是眼、肾脏、神经、心血管的长期损伤、功能缺陷和衰竭。

关键词：抗糖尿病药物慢性高血糖状态胰岛素分泌缺陷药理病理生理机制胰岛素作用血葡萄糖环境因素

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线间距对600 km/h高速磁浮列车明线交会气动性能的影响

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中南大学学报（自然科学版） 2023年第7期54卷 2952-2963页

作者：杨鹏杨明智张雷赵凡蔺童童马江川中南大学交通运输工程学院轨道交通安全教育部重点实验室湖南长沙410075 中国空气动力研究与发展中心计算空气动力研究所四川绵阳621000

为探明不同线间距下600 km/h高速磁浮列车明线交会时的气动特性,基于三维、非定常、可压缩的N-S方程和SST k-ω湍流模型,采用重叠网格技术,分析列车明线交会时的车身周围流场结构、列车交会压力波和列车侧向力,通过动模型试验来验证数... 详细信息

为探明不同线间距下600 km/h高速磁浮列车明线交会时的气动特性,基于三维、非定常、可压缩的N-S方程和SST k-ω湍流模型,采用重叠网格技术,分析列车明线交会时的车身周围流场结构、列车交会压力波和列车侧向力,通过动模型试验来验证数值模拟方法的准确性。研究结果表明:在不同线间距下,列车交会时的车身周围流场分布特征相似,随线间距增大,列车尾涡展向角逐渐增大,两交会侧车身之间流场的速度和压力不断减小;不同线间距下的列车压力波变化规律一致,压力波幅值与列车运行速度的二次方近似呈正比,当线间距由5.1 m分别增大至5.6 m和6.1 m时,压力波幅值分别减小28.2%和42.4%,且增大线间距对列车压力波正波缓解作用比负波的大,头波的缓解作用比尾波的大;列车交会过程中头车侧向力幅值比尾车和中间车的幅值大,增大线间距对尾车侧向力的缓解作用比头车和中间车的大,当线间距由5.1 m增大至6.1 m时,头车、中间车和尾车的侧向力幅值分别减小33.8%、34.1%和35.7%。

关键词：高速磁浮列车线间距明线交会流场结构气动特性

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STAT3-Dependent Effects of Polymeric Immunoglobulin Receptor in Regulating Interleukin-17 Signaling and Preventing Autoimmune Hepatitis

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Engineering 2024年第5期36卷 209-222页

作者： Ting Li tongtong Pan Nannan Zheng Xiong Ma Xiaodong Wang Fang Yan Huimian Jiang Yuxin Wang Hongwei lin Jing lin Huadong Zhang Jia Huang lingming Kong Anmin Huang Qingxiu Liu Yongping Chen Dazhi Chen Hepatology Diagnosis and Treatment Center The First Affiliated Hospital of Wenzhou Medical University&Zhejiang Provincial Key Laboratory for Accurate Diagnosis and Treatment of Chronic Liver DiseasesWenzhou 325035China Department of Clinical Medicine Hangzhou Medical CollegeHangzhou 310053China State Key Laboratory for Oncogenes and Related Genes Renji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200001China Wenzhou Medical University Wenzhou 325035China Division of Infectious Diseases Lishui People’s HospitalLishui 323000China

One-third of patients with autoimmune hepatitis(AIH)have cirrhosis at the time of *** relevance of these variables,although unknown,is believed to be critical in AIH because of suspected interactions between the gut microbiome and genetic *** of the gut flora and elevated polymeric immunoglobulin receptor(pIgR)levels have been observed in both patients and mouse ***,there is a direct relationship between pIgR expression and transaminase levels in patients with *** this study,we aimed to explore how pIgR influences the secretion of regenerating islet-derived 3 beta(Reg3b)and the flora composition in AIH using in vivo experiments involving patients with AIH and a concanavalin A-induced mouse model of ***3b expression was reduced in pIgR gene(Pigr)-knockout mice compared to that in wild-type mice,leading to increased microbiota ***,exogenous pIgR supplementation increased Reg3b expression and maintained microbiota *** sequencing revealed the participation of the interleukin(IL)-17 signaling pathway in the regulation of Reg3b through ***,the introduction of external pIgR could not restore the imbalance in gut microbiota in AIH,and the decrease in Reg3b expression was not apparent following the inhibition of signal transducer and activator of transcription 3(STAT3).In this study,pIgR facilitated the upregulation of Reg3b via the STAT3 pathway,which plays a crucial role in preserving the balance of the intestinal microbiota in *** this research,we discovered new molecular targets that can be used for the diagnosis and treatment of AIH.

关键词： Autoimmune hepatitis Polymeric immunoglobulin receptor Regenerating islet-derived 3 beta Intestinal microbiota Signal transducer and activator of transcription 3

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Millimeter waves in medical applications:status and prospects

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Intelligent Medicine 2024年第1期4卷 16-21页

作者： Honglin Wang lin Lu Pengran Liu Jiayao Zhang Songxiang Liu Yi Xie tongtong Huo Hong Zhou Mingdi Xue Ying Fang Jiaming Yang Zhewei Ye Department of Orthopedics Surgery Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei 430022China Departments of Orthopedics Renmin Hospital of Wuhan UniversityWuhanHubei 430060China

Millimeter waves are electromagnetic waves with wavelengths of 1–10 mm,which have characteristics of high frequency and short *** have gradually and widely been used in engineering and medical *** have identified studies related to millimeter waves in the biomedical field and summarized the biological effects of millimeter waves and their current status in medical ***,the shortcomings of existing studies and future developments were analyzed and discussed,with the aim of providing a reference for further research and development of millimeter waves in the medical field.

关键词： Millimeter wave Biological effects Medical applications Vital signs monitoring

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