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Non-destructive buffer enabling near-infrared-transparent inverted inorganic perovskite solar cells toward 1400 h light-soaking stable perovskite/Cu(In,Ga)Se_(2) tandem solar cells

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Journal of Energy Chemistry 2024年第10期97卷 622-629,I0013页

作者： Yu Zhang Zhaoheng Tang Zhongyang Zhang Jiahong Tang Minghua Li Siyuan Zhu Wenyan Tan Xi Jin tongsheng chen Jinsong Hu Chao Zhou Chunlei Yang Qijie Liang Xugang Guo Weimin Li Weiqiang chen Yan Jiang MOE Key Laboratory of Laser Life Science&Guangdong Provincial Key Laboratory of Laser Life Science Guangzhou Key Laboratory of Spectral Analysis and Functional ProbesCollege of BiophotonicsSouth China Normal UniversityGuangzhou 510631GuangdongChina school of Materials Science and Engineering Beijing Institute of TechnologyBeijing 100081China Songshan Lake Materials Laboratory Dongguan 523429GuangdongChina Department of Materials Science and Engineering Guangdong Provincial Key Laboratory of Functional Oxide Materials and DevicesSouthern University of Science and TechnologyShenzhen 518055GuangdongChina Beijing Advanced Innovation Center for Soft Matter Science and Engineering College of Chemical EngineeringCollege of Materials Science and EngineeringBeijing University of Chemical TechnologyBeijing 100029China Department of Physics Liaoning UniversityShenyang 110036LiaoningChina Beijing National Laboratory for Molecular Sciences(BNLMS) Institute of ChemistryChinese Academy of SciencesBeijing 100190China School of Physics and Materials Science Guangzhou UniversityGuangzhou 510006GuangdongChina Shenzhen Institute of Advanced Technology Chinese Academy of SciencesShenzhen 18055GuangdongChina

Near-infrared(NIR)transparent inverted all-inorganic perovskite solar cells(PSCs)are excellent top cell candidates in tandem *** essential challenge is the replacement of metal contacts with transparent conductive oxide(TCO)electrodes,which requires the introduction of a buffer layer to prevent sputtering *** this study,we show that the conventional buffers(i.e.,small organic molecules and atomic layer deposited metal oxides)used for organic-inorganic hybrid perovskites are not applicable to all-inorganic perovskites,due to non-uniform coverage of the vulnerable layers underneath,deterioration upon ion bombardment and moisture induced perovskite phase transition,A thin film of metal oxide nanoparticles by the spin-coating method serves as a non-destructive buffer layer for inorganic ***-inorganic inverted near-infrared-transparent PSCs deliver a PCE of 17.46%and an average transmittance of 73.7%between 780 and 1200 *** combination with an 18.56%Cu(In,Ga)Se_(2) bottom cell,we further demonstrate the first all-inorganic perovskite/CIGS 4-T tandem solar cell with a PCE of 24.75%,which exhibits excellent illumination stability by maintaining 86.7%of its initial efficiency after 1400 *** non-destructive buffer lays the foundation for efficient and stable NIR-transparent inverted inorganic perovskite solar cells and perovskite-based tandems.

关键词： CsPbI_(3)perovskite Inverted perovskite solar cells Tandem solar cells Buffer layer Stability

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H_(2)O_(2) INDUCES APOPTOSIS OF RABBIT CHONDROCYTES VIA BOTH THE EXTRINSIC AND THE CASPASE-INDEP ENDENT INTRINSIC PATHW AYS

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Journal of Innovative Optical Health Sciences 2013年第3期6卷 66-75页

作者： CAIPING ZHUANG XIAOPING WANG tongsheng chen Department of Pain Management The First Affiliated Hospital of Jinan University Guangzhou 510632 P.R.China MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science South China Normal University Guangzhou 510631 P.R.China

Ostcoarthritis(OA),one of the most common joint discases with unknown etiology,is charac-terized by the progressive destruction of articular cartilage and the apoptosis of *** purpose of this study is to elucidate the molecular mechanisms of H_(2)O_(2)-mediated rabbit chon-drocytes ***-8 assay showed that H_(2)O_(2) treatment induced a remarkable reduction of cell viability,which was further verified by the remarkable phosphatidylserine extemalization after H_(2)O_(2) treatment for 1 h,the typical characteristics of apoptosis.H_(2)O_(2) treatment induced a signifcant dysfunction of mitochondrial membrane potential(△ψm),but did not induce casapse-9 activation,indicating that H_(2)O_(2) treat ment induced caspase independent intrinsic apoptosis that was further verified by the fact that silencing of AIF but not inhibiting caspase-9 potently pre-vented H_(2)O_(2)-induced apoptosis.H_(2)O_(2) treatment induced a signifcant increase of caspase8 and-3 activation,and inhibition of caspase-8 or-3 significantly prevented H_(2)O_(2)-induced apoptosis,suggesting that the extrinsic pathway played an important ***,our findings demonstrate that H_(2)O_(2) induces apoptosis via both the casapse8-mediated extrinsic and the caspaseindependent intrinsic apoptosis pathways in ra bbit chondrocytes.

关键词： Chondrocytes apoptosis H_(2)O_(2) caspases AIF

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Interaction between Bax and Bcl-XL proteins confirmed by partial acceptor photobleaching-based FRET imaging

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Journal of Innovative Optical Health Sciences 2020年第3期3卷 81-91页

作者： Fangfang Yang Mengyan Du Xiaoping Wang tongsheng chen MOE Key Laboratory of Laser Life Science and College of Biophotonics South China Normal University Guangzhou 510631 P.R.China Department of Pain Management the First Affiliated Hospital of Jinan University Guangzhou 510630 P.R.China

Exact interaction mechanism between Bax and Bcl-XL,two key Bcl-2 family proteins,is an interesting and controversial *** acceptor photobleaching-based quantitative fluores-cence resonance energy transfer(FRET)measurement,PbFRET,is a widely used FRET quantification method in living *** this report,we implemented pixel-to-pixel PbFRET imaging on a wide-field microscope to map the FRET efficiency(E)images of single living HepG2 cells co-expressing CFP-Bax and *** E value between CFP-Bax and YFP-Bcl-XL was 4.59%in cytosol and 11.31%on mitochondria,conclusively indicating the direct interaction of the two proteins,and the interaction of the two proteins was strong on mitochondria and modest in cytosol.

关键词： Bax Bcl-XL protein-protein interaction FRET imaging living cells

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LIVE IMAGING OF XIAO-AI-PING-INDUCED CELL DEATH IN HUMAN LUNG ADENOCARCINOMA CELLS

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Journal of Innovative Optical Health Sciences 2008年第1期1卷 151-156页

作者： XIAO-PING WANG tongsheng chen LONGXIANG WANG LEI SUN MOE Key Libratory of Laser Life Science&Institute of Laser Life Science South China Normal UniversityGuangzhou 510631China Department of Anesthesiology The First Affiliated Hospital of Jinan UniversityGuangzhou 510632China

Xiao-Ai-Ping(XAP),a traditional oriental medicinal herb isolated from the stem of Marsdenia tenacissima(Roxb.)Wight et Arn.,has been shown to induce tumor cell *** this study,we used confocal fluorescence microscopy and fluorescence resonance energy transfer(FRET)techniques to study the molecular mechanism of XAPinduced apoptosis in single living human lung adenocarcinoma(ASTC-a-1)*** efficacious apoptosis was observed at 6 h after of 100μl XAP *** monitoring the dynamics of caspase-3 activation using FRET imaging in single living ASTC-a-1 cell expressing stably with SCAT3,a FRET probe,showed that XAP activated the caspase-3 at about 2 h after XAP *** data suggest that caspase-3 activation was involved in the XAP-induced apoptosis in ASTC-a-1 cells.

关键词： Xiao-Ai-Ping(XAP) apoptosis caspase-3 FRET

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An intravascular needle coated by ZnO nanoflowers for in vivo elimination of circulating tumor cells

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Nano Research 2023年第1期16卷 873-881页

作者： Jinqi Li Xinlei Li Yangfeng Zhang Kun Jin Ye Yuan Ruiqi Ming Yuhua Yang tongsheng chen MOE Key Laboratory of Laser Life Science&Institute of Laser Life Science College of BiophotonicsSouth China Normal UniversityGuangzhou 510631China Guangdong Provincial Key Laboratory of Laser Life Science College of BiophotonicsSouth China Normal UniversityGuangzhou 510631China SCNU Qingyuan Institute of Science and Technology Innovation Co. Ltd.South China Normal UniversityQingyuan 511500China Guangzhou Key Laboratory of Spectral Analysis and Functional Probes College of BiophotonicsSouth China Normal UniversityGuangzhou 510631China State Key Laboratory of Optoelectronic Materials and Technologies Nanotechnology Research CenterSchool of Materials Science&EngineeringSun Yat-sen UniversityGuangzhou 510275China

Circulating tumor cells(CTCs)are recognized as the main source of tumor recurrence and *** the CTCs in peripheral blood provides a new strategy to reduce the probability of recurrence or ***,we proposed a concept to eliminate CTCs by inserting a needle in the superficial blood *** the property of ZnO and the structure of nanoflowers,we designed a medical needle coated with ZnO nanoflowers(ZNFs),which killed about 90%of captured CTCs in vitro and prevented the injecting CTCs from spreading to lung tissue in BABL/c mouse *** in vitro and in vivo demonstrated that the CTCs not only were captured and killed,but also detached from the needle surface after dead,enabling the ZNFs needle continually eliminate ***,a theoretical model was presented to explain the penetration mechanism of cells by nanostructures,which indicated that nanoflowers structure can puncture CTCs more easily than vertical nanowire *** concept of inserting an intravascular needle provides a potential strategy to lower the concentration of CTCs in blood and reduce the probability of tumor recurrence or metastasis.

关键词： ZnO nanoflowers circulating tumor cells elimination cancer treatment theoretical model

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Live-cell super-resolution imaging unconventional dynamics and assemblies of nuclear pore complexes

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Biophysics Reports 2023年第4期9卷 206-214页

作者： Xianxin Ye Minzhu Guan Yaorong Guo Xiang Liu Kunhao Wang tongsheng chen Shiqun Zhao Liangyi chen National Biomedical Imaging Center State Key Laboratory of Membrane Biology Beijing Key Laboratory of Cardiometabolic Molecular Medicine Institute of Molecular Medicine College of Future Technology Peking University Key Laboratory of Laser Life Science Ministry of Education College of Biophotonics South China Normal University PKU-IDG/Mc Govern Institute for Brain Research

Super-resolution microscopy has promoted the development of cell biology, but imaging proteins with low copy numbers in cellular structures remains challenging. The limited number of designated proteins within nuclear pore complexes(NPCs) impedes continuous observation in live cells, although they are often used as a standard for evaluating various SR methods. To address this issue, we tagged POM121 with Halo-SiR and imaged it using structured illumination microscopy with sparse deconvolution(Sparse-SIM). Remarkably, POM121-SiR exhibited more than six-fold fluorescence intensity and four-fold enhanced contrast compared to the same protein labeled with tandem-linked m Cherry, while showing negligible photo-bleaching during SR imaging for 200 frames. Using this technique, we discovered various types of NPCs, including ring-like and cluster-like structures, and observed dynamic remodeling along with the sequential appearance of different Nup compositions. Overall, Halo-Si R with Sparse-SIM is a potent tool for extended SR imaging of dynamic structures of NPCs in live cells, and it may also help visualize proteins with limited numbers in general.

关键词： Super-resolution microscopy Sparse deconvolution Nuclear pore complexes Halo-SiR Dual-color imaging

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Superresolution live-cell imaging reveals that the localization of TMEM106B to filopodia in oligodendrocytes is compromised by the hypomyelination-related D252N mutation

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Science China(Life Sciences) 2023年第8期66卷 1858-1868页

作者： Shijia Xing Xiaolu Zheng Huifang Yan Yanquan Mo Ruoyu Duan Zhixing chen Kunhao Wang Kai Gao tongsheng chen Shiqun Zhao Jingmin Wang Liangyi chen National Biomedical Imaging Center State Key Laboratory of Membrane BiologyBeijing Key Laboratory of Cardiometabolic Molecular MedicineInstitute of Molecular MedicineCollege of Future TechnologyPeking UniversityBeijing 100871China Department of Pediatrics Peking University First HospitalBeijing 100034China PKU-IDG/McGovern Institute for Brain Research Beijing 100871China National Biomedical Imaging Center Institute of Molecular MedicineCollege of Future TechnologyPeking UniversityBeijing 100871China Key Laboratory of Laser Life Science Ministry of EducationCollege of BiophotonicsSouth China Normal UniversityGuangzhou 510631China National Center for Children’s Health Department of NeurologyBeijing Children’s HospitalCapital Medical UniversityBeijing 100045China

Hypomyelination leukodystrophies constitute a group of heritable white matter disorders exhibiting defective myelin *** identified as a lysosomal protein,the TMEM106B D252N mutant has recently been associated with ***,how lysosomal TMEM106B facilitates myelination and how the D252N mutation disrupts that process are poorly *** used superresolution Hessian structured illumination microscopy(Hessian-SIM)and spinning discconfocal structured illumination microscopy(SD-SIM)to find that the wild-type TMEM106B protein is targeted to the plasma membrane,filopodia,and lysosomes in human *** D252N mutation reduces the size of lysosomes in oligodendrocytes and compromises lysosome changes upon starvation *** importantly,we detected reductions in the length and number of filopodia in cells expressing the D252N ***1 is the most abundant myelin protein that almost entirely colocalizes with TMEM106B,and coexpressing PLP1 with the D252N mutant readily rescues the lysosome and filopodia phenotypes of ***,interactions between TMEM106B and PLP1 on the plasma membrane are essential for filopodia formation and myelination in oligodendrocytes,which may be sustained by the delivery of these proteins from lysosomes via exocytosis.

关键词： superresolution imaging oligodendrocyte TMEM106B lysosome filopodia

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Fluorescence Resonance Energy Transfer Analysis of Bid Activation in Living Cells during Ultraviolet-induced Apoptosis

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Acta Biochimica et Biophysica Sinica 2007年第1期39卷 37-45页

作者： Yinyuan WU Da XING Lei LIU tongsheng chen and Wei R.chen MOE Key Laboratory of Laser Life Science &Institute of Life Science,South China Normal University,Guangzhou 510631,China MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science South China Normal UniversityGuangzhou 510631 China

Ultraviolet (UV) irradiation is a DNA-damaging agent that triggers apoptosis through both themembrane death receptor and mitochondrial apoptotic signaling ***,a pro-apoptotic Bcl-2family member,is important in most cell types to apoptosis in response to DNA *** this study,arecombinant plasmid,YFP-Bid-CFP,comprised of yellow and cyan fluorescent protein and a full length Bid,was used as a fluorescence resonance energy transfer analysis (FRET) *** the FRET techniquebased on YFP-Bid-CFP,we found that Bid activation was initiated at 9±1 h after UV irradiation,and theaverage duration of the activation was 75±10 *** activation coincided with a collapse of the mitochondrialmembrane potential with an average duration of 50±10 min. When cells were pretreated with Z-IETD-fmk(caspase-8 specific inhibitor) the process of Bid activation was completely inhibited,but the apoptosis wasonly partially affected.Z-DEVD-fmk (caspase-3 inhibitor) and Z-FA-fmk (non asp specific inhibitor) didnot block Bid ***,the endogenous Bid activation with or without Z-IETD-fmk in responseto UV irradiation was confirmed by Western *** summary, using the FRET technique,we observedthe dynamics of Bid activation during UV-induced apoptosis and found that it was a caspase-8 dependentevent.

关键词： ultraviolet irradiation Bid activation apoptosis caspase-8 fluorescence resonance energy transfer analysis

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Structured illumination-based super-resolution live-cell quantitative FRET imaging

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Photonics Research 2023年第5期11卷 887-896页

作者： ZEWEI LUO GE WU MENGTING KONG ZHI chen ZHENGFEI ZHUANG JUNCHAO FAN tongsheng chen Key Laboratory of Laser Life Science Ministry of EducationCollege of BiophotonicsSouth China Normal UniversityGuangzhou 510631China Guangdong Key Laboratory of Laser Life Science College of BiophotonicsSouth China Normal UniversityGuangzhou 510631China SCNU Qingyuan Institute of Science and Technology Innovation South China Normal UniversityQingyuan 511520China Chongqing Key Laboratory of Image Cognition College of Computer Science and TechnologyChongqing University of Posts and TelecommunicationsChongqing 400065China

Forster resonance energy transfer(FRET)microscopy provides unique insight into the functionality of biological systems via imaging the spatiotemporal interactions and functional state of *** FRET signals from sub-diffraction regions requires super-resolution(SR)FRET imaging,yet is challenging to achieve from living ***,we present an SR FRET method named SIM-FRET that combines SR structured illumination microscopy(SIM)imaging and acceptor sensitized emission FRET imaging for live-cell quantitative SR FRET *** the robust co-localization prior of donor and accepter during FRET,we devised a mask filtering approach to mitigate the impact of SIM reconstruction artifacts on quantitative FRET *** to wide-field FRET imaging,SIM-FRET provides nearly twofold spatial resolution enhancement of FRET imaging at sub-second timescales and maintains the advantages of quantitative FRET analysis in *** validate the resolution enhancement and quantitative analysis fidelity of SIM-FRET signals in both simulated FRET models and live-cell FRET-standard construct *** method reveals the intricate structure of FRET signals,which are commonly distorted in conventional wide-field FRET imaging.

关键词： illumination resolution donor

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Automated apoptosis identification in fluorescence imaging of nucleus based on histogram of oriented gradients of high-frequency wavelet coefficients

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Journal of Innovative Optical Health Sciences 2023年第2期16卷 28-38页

作者： Shutong Liu Limei Su Han Sun tongsheng chen Min Hu Zhengfei Zhuang MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science College of Biophotonics South China Normal University Guangzhou 510631P.R.China Guangdong Provincial Key Laboratory of Laser Life Science College of Biophotonics South China Normal University Guangzhou 510631P.R.China Guangdong Provincial Key Laboratory of Nanophotonic Functional Materials and Devices School of Information and Optoelectronic Science and Engineering South China Normal University Guangzhou 510631 P.R.China SCNU Qingyuan Institute of Science and Technology Innovation Co. Ltd.Qingyuan 511500P.R.China

The automatic and accurate identification of apoptosis facilitates large-scale cell *** identification approaches using nucleus fluorescence imaging are based on specific morphological ***,these parameters cannot completely describe nuclear morphology,thus limiting the identification accuracy of *** paper proposes a new feature extraction method to improve the performance of the model for apoptosis *** proposed method uses a histogram of oriented gradient(HOG)of high-frequency wavelet coefficients to extract internal and edge texture *** HOG vectors are classified using support vector *** experimental results demonstrate that the proposed feature extraction method well performs apoptosis identification,attaining 95:7% accuracy with low cost in terms of *** confirmed that our method has potential applications to cell biology research.

关键词： Apoptosis nucleus fluorescence imaging HOG wavelet decomposition

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