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H_(2)O_(2) INDUCES APOPTOSIS OF RABBIT CHONDROCYTES VIA BOTH THE EXTRINSIC AND THE CASPASE-INDEP ENDENT INTRINSIC PATHW AYS

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Journal of Innovative Optical Health Sciences 2013年第3期6卷 66-75页

作者： CAIPING ZHUANG XIAOPING WANG Tongsheng Chen Department of Pain Management The First Affiliated Hospital of Jinan University Guangzhou 510632 P.R.China MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science South China Normal University Guangzhou 510631 P.R.China

Ostcoarthritis(OA),one of the most common joint discases with unknown etiology,is charac-terized by the progressive destruction of articular cartilage and the apoptosis of chondrocytes.The purpose of this study is to elucidate the molecular mechanisms of H_(2)O_(2)-mediated rabbit chon-drocytes apoptosis.CCK-8 assay showed that H_(2)O_(2) treatment induced a remarkable reduction of cell viability,which was further verified by the remarkable phosphatidylserine extemalization after H_(2)O_(2) treatment for 1 h,the typical characteristics of apoptosis.H_(2)O_(2) treatment induced a signifcant dysfunction of mitochondrial membrane potential(△ψm),but did not induce casapse-9 activation,indicating that H_(2)O_(2) treat ment induced caspase independent intrinsic apoptosis that was further verified by the fact that silencing of AIF but not inhibiting caspase-9 potently pre-vented H_(2)O_(2)-induced apoptosis.H_(2)O_(2) treatment induced a signifcant increase of caspase8 and-3 activation,and inhibition of caspase-8 or-3 significantly prevented H_(2)O_(2)-induced apoptosis,suggesting that the extrinsic pathway played an important role.Collectively,our findings demonstrate that H_(2)O_(2) induces apoptosis via both the casapse8-mediated extrinsic and the caspaseindependent intrinsic apoptosis pathways in ra bbit chondrocytes.

关键词： Chondrocytes apoptosis H_(2)O_(2) caspases AIF

An intravascular needle coated by ZnO nanoflowers for in vivo elimination of circulating tumor cells

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Nano Research 2023年第1期16卷 873-881页

作者： Jinqi Li Xinlei Li Yangfeng Zhang Kun Jin Ye Yuan Ruiqi Ming Yuhua Yang Tongsheng Chen MOE Key Laboratory of Laser Life Science&Institute of Laser Life Science College of BiophotonicsSouth China Normal UniversityGuangzhou 510631China Guangdong Provincial Key Laboratory of Laser Life Science College of BiophotonicsSouth China Normal UniversityGuangzhou 510631China SCNU Qingyuan Institute of Science and Technology Innovation Co. Ltd.South China Normal UniversityQingyuan 511500China Guangzhou Key Laboratory of Spectral Analysis and Functional Probes College of BiophotonicsSouth China Normal UniversityGuangzhou 510631China State Key Laboratory of Optoelectronic Materials and Technologies Nanotechnology Research CenterSchool of Materials Science&EngineeringSun Yat-sen UniversityGuangzhou 510275China

Circulating tumor cells(CTCs)are recognized as the main source of tumor recurrence and metastasis.Eliminating the CTCs in peripheral blood provides a new strategy to reduce the probability of recurrence or metastasis.Here,we proposed a concept to eliminate CTCs by inserting a needle in the superficial blood vessel.Using the property of ZnO and the structure of nanoflowers,we designed a medical needle coated with ZnO nanoflowers(ZNFs),which killed about 90%of captured CTCs in vitro and prevented the injecting CTCs from spreading to lung tissue in BABL/c mouse model.Results in vitro and in vivo demonstrated that the CTCs not only were captured and killed,but also detached from the needle surface after dead,enabling the ZNFs needle continually eliminate CTCs.Furthermore,a theoretical model was presented to explain the penetration mechanism of cells by nanostructures,which indicated that nanoflowers structure can puncture CTCs more easily than vertical nanowire structure.The concept of inserting an intravascular needle provides a potential strategy to lower the concentration of CTCs in blood and reduce the probability of tumor recurrence or metastasis.

关键词： ZnO nanoflowers circulating tumor cells elimination cancer treatment theoretical model

Superresolution live-cell imaging reveals that the localization of TMEM106B to filopodia in oligodendrocytes is compromised by the hypomyelination-related D252N mutation

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Science China(Life Sciences) 2023年第8期66卷 1858-1868页

作者： Shijia Xing Xiaolu Zheng Huifang Yan Yanquan Mo Ruoyu Duan Zhixing chen Kunhao Wang Kai Gao Tongsheng Chen Shiqun Zhao Jingmin Wang Liangyi chen National Biomedical Imaging Center State Key Laboratory of Membrane BiologyBeijing Key Laboratory of Cardiometabolic Molecular MedicineInstitute of Molecular MedicineCollege of Future TechnologyPeking UniversityBeijing 100871China Department of Pediatrics Peking University First HospitalBeijing 100034China PKU-IDG/McGovern Institute for Brain Research Beijing 100871China National Biomedical Imaging Center Institute of Molecular MedicineCollege of Future TechnologyPeking UniversityBeijing 100871China Key Laboratory of Laser Life Science Ministry of EducationCollege of BiophotonicsSouth China Normal UniversityGuangzhou 510631China National Center for Children’s Health Department of NeurologyBeijing Children’s HospitalCapital Medical UniversityBeijing 100045China

Hypomyelination leukodystrophies constitute a group of heritable white matter disorders exhibiting defective myelin development.Initially identified as a lysosomal protein,the TMEM106B D252N mutant has recently been associated with hypomyelination.However,how lysosomal TMEM106B facilitates myelination and how the D252N mutation disrupts that process are poorly understood.We used superresolution Hessian structured illumination microscopy(Hessian-SIM)and spinning discconfocal structured illumination microscopy(SD-SIM)to find that the wild-type TMEM106B protein is targeted to the plasma membrane,filopodia,and lysosomes in human oligodendrocytes.The D252N mutation reduces the size of lysosomes in oligodendrocytes and compromises lysosome changes upon starvation stress.Most importantly,we detected reductions in the length and number of filopodia in cells expressing the D252N mutant.PLP1 is the most abundant myelin protein that almost entirely colocalizes with TMEM106B,and coexpressing PLP1 with the D252N mutant readily rescues the lysosome and filopodia phenotypes of cells.Therefore,interactions between TMEM106B and PLP1 on the plasma membrane are essential for filopodia formation and myelination in oligodendrocytes,which may be sustained by the delivery of these proteins from lysosomes via exocytosis.

关键词： superresolution imaging oligodendrocyte TMEM106B lysosome filopodia

High-Order Characteristic Modes Suppression for Cross-Band Decoupling in Shared-Aperture Antenna Array

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Chinese Journal of Electronics 2022年第6期31卷 1121-1128页

作者： LU Xiaochi chen Yikai SHEN tongsheng GUO Shaojun ZOU Chunrong YANG Shiwen School of Electronic Science and Engineering University of Electronic Science and Technology of China National Innovation Institute of Defense Technology Academy of Military Sciences PLA China

In dual-band shared-aperture base station antennas, the secondary radiation(SR) field caused by cross-band couplings always leads to radiation pattern distortion. In this work, the SR field caused by the coupling is attributed to the excitation of the target highorder modes from the perspective of the characteristic mode. To effectively suppress the target modes, L-shaped slots are proposed to load the arms of the radiator and altering the current paths. As compared with conventional dipoles without L-shaped slots, the proposed antenna generates less SR field. Therefore, the radiation pattern of the high-band antennas keeps stable in a dual-band shared-aperture antenna array environment. Prototype of a dual-band antenna array consisting of the proposed lowband(LB) antenna and a 4 × 4 high-band(HB) antenna array is fabricated. Simulated and measured results show that the designed dual-band array operates at both 0.74–0.82 GHz and 3.30–3.80 GHz. The experimental results verify that the cross-band decoupling has been achieved with the high-order mode suppression.

关键词： Characteristic modes (CMs) Base station antenna Shared-aperture antenna High-order modes suppression

A method for identifying coalbed methane co-production interference based on production characteristic curves: A case study of the Zhijin block, western Guizhou, China

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Petroleum Exploration and Development 2022年第5期49卷 1126-1137页

作者： GUO chen QIN Yong YI tongsheng chen Zhenlong YUAN Hang GAO Junzhe GOU Jiang College of Geology and Environment Xi'an University of Science and TechnologyXi'an 710054China Shaanxi Provincial Key Laboratory of Geological Support for Coal Green Exploitation Xi'an 710054China Geological Research Institute for Coal Green Mining Xi'an University of Science and TechnologyXi'an 710054China Key Laboratory of CBM Resources and Reservoir Formation Process Ministry of EducationChina University of Mining and TechnologyXuzhou 221116China Guizhou Bureau of Coal Geological Exploration Guiyang 550008China East China Oil&Gas Company SinopecNanjing 210011China

Efficient detection of coalbed methane(CBM) co-production interference is the key to timely adjusting the development plan and improving the co-production efficiency. Based on production data of six typical CBM co-production wells in the Zhijin block of western Guizhou Province, China, the production characteristic curves, including production indication curve, curve of daily water production per unit drawdown of producing fluid level with time, and curve of water production per unit differential pressure with time have been analyzed to explore the response characteristics of co-production interference on the production characteristic curves. Based on the unit water inflow data of pumping test in coal measures, the critical value of in-situ water production of the CBM wells is 2 m^(3)/(d·m). The form and the slope of the initial linear section of the production indication curves have clear responses to the interference, which can be used to discriminate internal water source from external water source based on the critical slope value of 200 m^(3)/MPa in the initial linear section of the production indication curve. The time variation curves of water production per unit differential pressure can be divided into two morphological types: up-concave curve and down-concave curve. The former is represented by producing internal water with average daily gas production greater than 800 m^(3)/d, and the latter produces external water with average daily gas production smaller than 400 m^(3)/d. The method and critical indexes for recognition of CBM co-production interference based on the production characteristic curve are constructed. A template for discriminating interference of CBM co-production was constructed combined with the gas production efficiency analysis, which can provide reference for optimizing co-production engineering design and exploring economic and efficient co-production mode.

关键词： coalbed methane multi-seam co-production interlayer interference production indication curve external water internal water discrimination template

Structured illumination-based super-resolution live-cell quantitative FRET imaging

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Photonics Research 2023年第5期11卷 887-896页

作者： ZEWEI LUO GE WU MENGTING KONG ZHI chen ZHENGFEI ZHUANG JUNCHAO FAN Tongsheng Chen Key Laboratory of Laser Life Science Ministry of EducationCollege of BiophotonicsSouth China Normal UniversityGuangzhou 510631China Guangdong Key Laboratory of Laser Life Science College of BiophotonicsSouth China Normal UniversityGuangzhou 510631China SCNU Qingyuan Institute of Science and Technology Innovation South China Normal UniversityQingyuan 511520China Chongqing Key Laboratory of Image Cognition College of Computer Science and TechnologyChongqing University of Posts and TelecommunicationsChongqing 400065China

Forster resonance energy transfer(FRET)microscopy provides unique insight into the functionality of biological systems via imaging the spatiotemporal interactions and functional state of proteins.Distinguishing FRET signals from sub-diffraction regions requires super-resolution(SR)FRET imaging,yet is challenging to achieve from living cells.Here,we present an SR FRET method named SIM-FRET that combines SR structured illumination microscopy(SIM)imaging and acceptor sensitized emission FRET imaging for live-cell quantitative SR FRET imaging.Leveraging the robust co-localization prior of donor and accepter during FRET,we devised a mask filtering approach to mitigate the impact of SIM reconstruction artifacts on quantitative FRET analysis.Compared to wide-field FRET imaging,SIM-FRET provides nearly twofold spatial resolution enhancement of FRET imaging at sub-second timescales and maintains the advantages of quantitative FRET analysis in vivo.We validate the resolution enhancement and quantitative analysis fidelity of SIM-FRET signals in both simulated FRET models and live-cell FRET-standard construct samples.Our method reveals the intricate structure of FRET signals,which are commonly distorted in conventional wide-field FRET imaging.

关键词： illumination resolution donor

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论商周文献中祖宗神形象的演变

世界宗教文化 2022年第3期 13-20页

作者：陈桐生广东外语外贸大学中文学院

商周人根据自己对神灵的理解在各类文献中构建祖宗神形象。殷商文献中的祖宗神形象具有两面性,一方面会保佑、赐福子孙,另一方面又会惩诫、加害后人。西周初,周武王重病,周公祷告太王、王季、文王在天之灵,祈求祖灵护佑武王康复,其祷辞... 详细信息

商周人根据自己对神灵的理解在各类文献中构建祖宗神形象。殷商文献中的祖宗神形象具有两面性,一方面会保佑、赐福子孙,另一方面又会惩诫、加害后人。西周初,周武王重病,周公祷告太王、王季、文王在天之灵,祈求祖灵护佑武王康复,其祷辞载于《尚书·金縢》。此文扭转了周人心目中的祖宗神形象,在商周人对祖宗神的认识史上具有重要意义。《金縢》以后,西周各类文献中的祖宗神多具护佑、赐福功能,鲜有贻害子孙的情形。

关键词：甲骨卜辞祖宗神形象《金縢》赐福祟害

HSPOG:An Optimized Target Recognition Method Based on Histogram of Spatial Pyramid Oriented Gradients

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Tsinghua Science and Technology 2021年第4期26卷 475-483页

作者： Shaojun Guo Feng Liu Xiaohu Yuan Chunrong Zou Li chen tongsheng Shen National Innovation of Defense Technology Academy of Military Sciences PLA ChinaBeijing 100071China Department of Automation Tsinghua UniversityBeijing 100084China

The Histograms of Oriented Gradients(HOG)can produce good results in an image target recognition mission,but it requires the same size of the target images for classification of inputs.In response to this shortcoming,this paper performs spatial pyramid segmentation on target images of any size,gets the pixel size of each image block dynamically,and further calculates and normalizes the gradient of the oriented feature of each block region in each image layer.The new feature is called the Histogram of Spatial Pyramid Oriented Gradients(HSPOG).This approach can obtain stable vectors for images of any size,and increase the target detection rate in the image recognition process significantly.Finally,the article verifies the algorithm using VOC2012 image data and compares the effect of HOG.

关键词： Histograms of Oriented Gradients(HOG) Histogram of Spatial Pyramid Oriented Gradients(HSPOG) object recognition spatial pyramid segmentation

Automated apoptosis identification in fluorescence imaging of nucleus based on histogram of oriented gradients of high-frequency wavelet coefficients

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Journal of Innovative Optical Health Sciences 2023年第2期16卷 28-38页

作者： Shutong Liu Limei Su Han Sun Tongsheng Chen Min Hu Zhengfei Zhuang MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science College of Biophotonics South China Normal University Guangzhou 510631P.R.China Guangdong Provincial Key Laboratory of Laser Life Science College of Biophotonics South China Normal University Guangzhou 510631P.R.China Guangdong Provincial Key Laboratory of Nanophotonic Functional Materials and Devices School of Information and Optoelectronic Science and Engineering South China Normal University Guangzhou 510631 P.R.China SCNU Qingyuan Institute of Science and Technology Innovation Co. Ltd.Qingyuan 511500P.R.China

The automatic and accurate identification of apoptosis facilitates large-scale cell analysis.Most identification approaches using nucleus fluorescence imaging are based on specific morphological parameters.However,these parameters cannot completely describe nuclear morphology,thus limiting the identification accuracy of models.This paper proposes a new feature extraction method to improve the performance of the model for apoptosis identification.The proposed method uses a histogram of oriented gradient(HOG)of high-frequency wavelet coefficients to extract internal and edge texture information.The HOG vectors are classified using support vector machine.The experimental results demonstrate that the proposed feature extraction method well performs apoptosis identification,attaining 95:7% accuracy with low cost in terms of time.We confirmed that our method has potential applications to cell biology research.

关键词： Apoptosis nucleus fluorescence imaging HOG wavelet decomposition