检索结果-南通市图书馆

VGLUT3 neurons in median raphe control the efficacy of spatial memory retrieval via ETV4 regulation of VGLUT3 transcription

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2022年第8期65卷 1590-1607页

作者： Aodi He Chen zhang Xiao Ke Yao Yi Quntao Yu tongmei zhang Hongyan Yu Huiyun Du Hao Li Qing Tian Ling-Qiang Zhu Youming Lu Department of Physiology School of Basic Medicine and Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Wuhan Center for Brain Science Huazhong University of Science and TechnologyWuhan 430030China Department of Pathophysiology School of Basic MedicineHuazhong University of Science and TechnologyWuhan 430030China

The raphe nucleus is critical for feeding, rewarding and memory. However, how the heterogenous raphe neurons are molecularly and structurally organized to engage their divergent functions remains unknown. Here, we genetically target a subset of neurons expressing VGLUT3. VGLUT3 neurons control the efficacy of spatial memory retrieval by synapsing directly with parvalbumin-expressing GABA interneurons(PGIs) in the dentate gyrus. In a mouse model of Alzheimer's disease(AD mice),VGLUT3→PGIs synaptic transmission is impaired by ETV4 inhibition of VGLUT3 transcription. ETV4 binds to a promoter region of VGLUT3 and activates VGLUT3 transcription in VGLUT3 neurons. Strengthening VGLUT3→PGIs synaptic transmission by ETV4 activation of VGLUT3 transcription upscales the efficacy of spatial memory retrieval in AD mice. This study reports a novel circuit and molecular mechanism underlying the efficacy of spatial memory retrieval via ETV4 inhibition of VGLUT3 transcription and hence provides a promising target for therapeutic intervention of the disease progression.

关键词： VGLUT3 median raphe nucleus parvalbumin-expressing GABA interneurons spatial memory retrieval ETV4 Alzheimer's disease

(Tn5-)FISH-based imaging in the era of 3D/spatial genomics

在线全文

学校读者我要写书评

暂无评论

Biophysics Reports 2023年第1期9卷 15-25页

作者： Liheng Yang Yan Yan Jun Lin Li Cheng Zhou Jinlan Jin tongmei zhang Haokaifeng Wu Xingang Li Wei Wang Li Yuan Xu zhang Juntao Gao Seaver College Pepperdine University Center for Synthetic & Systems Biology Tsinghua University 3. Bioinformatics Division BNRist Department of Automation MOE Key Laboratory of Bioinformatics Savaid Medical School University of Chinese Academy of Sciences Department of Radiation Oncology Nanfang Hospital Southern Medical University Department of Critical Care Medicine Shenzhen Hospital (Futian) of Guangzhou University of Chinese Medicine 8. Medical Oncology Beijing Chest Hospital Capital Medical University & Beijing Tuberculosis and Thoracic Tumor Research Institute Centre for Regenerative Medicine and Health Hong Kong Institute of Science & Innovation Chinese Academy of Sciences Key Laboratory of Biological Targeting Diagnosis Therapy and Rehabilitation of Guangdong Higher Education Institutes The Fifth Affiliated Hospital of Guangzhou Medical University Centre for Precision Health Edith Cowan University Beijing Institute of Collaborative Innovation Institute for TCM-X

3D genomics mainly focuses on the 3D position of single genes at the cell level, while spatial genomics focuses more on the tissue level. In this exciting new era of 3D/spatial genomics, half-century old FISH and its derivative methods, including Tn5-FISH, play important roles. In this review, we introduce the Tn5-FISH we developed recently, and present six different applications published by our collaborators and us, based on(Tn5-)FISH, which can be either general BAC clone-based FISH or Tn5-FISH. In these interesting cases,(Tn5-)FISH demonstrated its vigorous ability of targeting sub-chromosomal structures across different diseases and cell lines(leukemia, m ESCs(mouse embryonic stem cells), and differentiation cell lines). Serving as an effective tool to image genomic structures at the kilobase level,Tn5-FISH holds great potential to detect chromosomal structures in a high-throughput manner, thus bringing the dawn for new discoveries in the great era of 3D/spatial genomics.

关键词： Tn5-FISH Molecular imaging Chromatin interaction Regulatory elements

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

早产儿视网膜病变的相关信号通路

中华实验眼科杂志 2015年第7期33卷 664-668页

作者：张桐梅韩梅天津市眼科医院天津医科大学眼科临床学院天津市眼科研究所天津市眼科学与视觉科学重点实验室 300020

早产儿视网膜病变（ROP）是发生在早产儿的一种血管增生性的玻璃体视网膜疾病,其主要病理性改变是视网膜血管闭塞、缺血缺氧诱发病理性新生血管形成,进而导致视网膜脱离,甚至失明.这类增生性血管病变涉及多条信号通路,对于分子机制的深... 详细信息

早产儿视网膜病变（ROP）是发生在早产儿的一种血管增生性的玻璃体视网膜疾病,其主要病理性改变是视网膜血管闭塞、缺血缺氧诱发病理性新生血管形成,进而导致视网膜脱离,甚至失明.这类增生性血管病变涉及多条信号通路,对于分子机制的深入理解更有利于靶向治疗病理性新生血管.就ROP发病机制中的有关信号通路进行综述,主要包括Wnt-信号通路成员在病理性血管发展中的作用,CCN1蛋白与富含半胱氨酸蛋白61（CCN1/Cry61）在提高视网膜血管的生理性适应和减少病理性新生血管生成方面的重要作用,Janus激酶/信号转导子与转录激活子（JAK/STAT）信号通路诱导视网膜和玻璃体内新生血管的形成,Apelin蛋白与血管紧张素1型受体相关蛋白（Apelin/A PJ）信号通路对于视网膜血管发生、血管塑形的影响以及在低氧条件下引发新生血管形成.

关键词：视网膜病变,早产儿高氧视网膜新生血管化信号通路

Disseminated tumor cells in bone marrow as predictive classifiers for small cell lung cancer patients

在线全文

学校读者我要写书评

暂无评论

Journal of the National Cancer Center

Journal of the National Cancer Center 2024年第4期4卷 335-345页

作者： Ying Wang Jingying Nong Baohua Lu Yuan Gao Mingming Hu Cen Chen Lina zhang Jinjing Tan Xiaomei Yang Peter Ping Lin Xingsheng Hu tongmei zhang Department of Medical Oncology Beijing Chest HospitalCapital Medical UniversityBeijing Tuberculosis and Thoracic Tumor Research InstituteBeijingChina Department of Thoracic Surgery Xuanwu HospitalCapital Medical UniversityBeijingChina The First School of Clinical Medicine Shaanxi University of Chinese MedicineXianyangChina Department of Cancer Research Center Beijing Chest HospitalCapital Medical UniversityBeijing Tuberculosis and Thoracic Tumor Research InstituteBeijingChina Beijing Key Laboratory for Tumor Invasion and Metastasis Department of Biochemistry and Molecular BiologyCapital Medical UniversityBeijingChina Joint Laboratory for Precision Diagnosis and Treatment Translational Research in Malignant Tumors Gynecologic Oncology Basic and Clinical Research LaboratoryCapital Medical UniversityBeijingChina Cytelligen San DiegoUSA Department of Medical Oncology National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijingChina Laboratory for Clinical Medicine Capital Medical UniversityBeijingChina

Background: Small cell lung cancer (SCLC) is a highly aggressive disease characterized by early metastasis. Ane- uploid CD31- disseminated tumor cells (DTCs) and CD31+ disseminated tumor endothelial cells (DTECs) residing in the bone marrow are generally considered as the initiators of metastatic process. However, the clinical signifi- cance of DTCs and DTECs in SCLC remains poorly understood. The aim of this study is to investigate the clinical implications of diverse subtypes of highly heterogeneous DTCs and DTECs in SCLC patients. Methods: Subtraction enrichment and immunostaining-fluorescence in situ hybridization (SE-iFISH) was applied to enrich and perform comprehensive morphologic, karyotypic, and phenotypic characterization of aneuploid DTCs and DTECs in 30 patients. Additionally, co-detection of circulating tumor cells (CTCs) and circulating tumor endothelial cells (CTECs) was conducted on 24 of the enrolled patients. Proof-of-concept of the whole exon sequencings (WES) on precisely selected different subtypes of CTCs or DTCs, longitudinally detected from a representative case with pathologically confirmed bone marrow metastasis, was validated to feasibly reveal genetic mutations in these cells. Results: DTCs, DTECs and their subtypes were readily detectable in SCLC patients. Comparative analysis re- vealed that the number of DTCs and DTECs was significantly higher than that of their corresponding CTCs and CTECs ( P < 0.001 for both). Positive detection of disseminated tumor microemboli (DTM) or disseminated tumor endothelial microemboli (DTEM) was associated with inferior survival outcomes ( P = 0.046 and P = 0.048). Pa- tients with EpCAM+ DTCs detectable displayed significantly lower disease control rate (DCR) (16.67% vs 73.33%, P = 0.019), reduced median progression-free survival (mPFS) and median overall survival (mOS) compared with those with EpCAM- DTCs ( P = 0.028 and P = 0.002, respectively). WES analysis indicated that post-treatment DTCs isolated

关键词： Bone marrow Aneuploid DTCs and DTECs SE-iFISH Prognosis SCLC

PINK1-mediated Drp1^(S616) phosphorylation modulates synaptic development and plasticity via promoting mitochondrial fission

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Signal Transduction and Targeted Therapy 2022年第5期7卷 1719-1734页

作者： Qingtao Gao Runyi Tian Hailong Han Jesse Slone Caifang Wang Xiao Ke tongmei zhang Xiangyu Li Yuhong He Panlin Liao Fang Wang Ye Chen Shiqing Fu Kexuan zhang Fangfang Zeng Yingxuan Yang Zhuo Li Jieqiong Tan Jiada Li Youming Lu Taosheng Huang Zhonghua Hu Zhuohua zhang Institute of Molecular Precision Medicine Xiangya HospitalKey Laboratory of Molecular Precision Medicine of Hunan Province and Center for Medical GeneticsHunan Key Laboratory of Medical GeneticsCentral South UniversityChangshaHunan 410008China Department of Pediatrics Jacobs School of Medicine and Biomedical SciencesUniversity at BuffaloBuffaloNew York 14203USA Department of Physiology School of Basic Medicine and Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei 430030China The Institute for Brain Research Collaborative Innovation Center for Brain ScienceHuazhong University of Science and TechnologyWuhanHubei 430030China Hunan Key Laboratory of Animal Models for Human Diseases School of Life SciencesCentral South UniversityChangshaHunan 410008China Department of Neurosciences University of South China Medical SchoolHengyangHunan 421001China National Clinical Research Center for Geriatric Disorders Xiangya HospitalCentral South UniversityChangshaHunan 410008China Department of Critical Care Medicine Hunan Provincial Clinical Research Center for Critical Care MedicineXiangya HospitalCentral South UniversityChangshaHunan 410008China

Dynamic change of mitochondrial morphology and distribution along neuronal branches are essential for neural circuitry formation and synaptic ***,the underlying mechanism remains *** show here that Pink1 knockout(KO)mice display defective dendritic spine maturation,reduced axonal synaptic vesicles,abnormal synaptic connection,and attenuated long-term synaptic potentiation(LTP).Drp1 activation via ^(S616) phosphorylation rescues deficits of spine maturation in Pink1 KO neurons.

关键词： plasticity activation synaptic

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

银屑病313例临床与组织病理分析

中华皮肤科杂志 2018年第10期51卷 741-744页

作者：张同梅王刚第四军医大学西京皮肤医院西安710032

目的分析以皮肤表现为主的6型银屑病的临床表现和组织病理学特征。方法回顾分析2013--2017年西京皮肤医院收治的313例银屑病患者的临床和组织病理资料。结果滴状银屑病31例，主要诱因为上呼吸道感染。斑块状银屑病60例，主要诱因有上... 详细信息

目的分析以皮肤表现为主的6型银屑病的临床表现和组织病理学特征。方法回顾分析2013--2017年西京皮肤医院收治的313例银屑病患者的临床和组织病理资料。结果滴状银屑病31例，主要诱因为上呼吸道感染。斑块状银屑病60例，主要诱因有上呼吸道感染、劳累、寒冷等。红皮病性银屑病42例，主要诱因为系统使用糖皮质激素的撤药。泛发性脓疱性银屑病60例，诱因有上呼吸道感染、妊娠、药物等。掌跖脓疱病60例，大部分无明确诱因。连续性肢端皮炎60例，大部分无明确诱因。6型银屑病具有相似的典型病理表现，包括角化不全、Munro微脓疡和／或Kogoj海绵状脓疡、颗粒层变薄或消失、表皮银屑病样增生、真皮乳头血管扩张、淋巴细胞浸润等。所有病例中98例（31％）出现不典型病理表现，其中滴状4例（13％），斑块状3例（5％），红皮病性12例（29％），泛发性脓疱性41例（68％），掌跖脓疱病12例（20％），连续性肢端皮炎26例（43％），包括浆液渗出、表皮不规则增生、非中性粒细胞性海绵水肿、角质形成细胞坏死、真皮中性粒细胞浸润、真皮嗜酸性粒细胞浸润等。结论各型银屑病具有不同的临床表现和相似的典型组织病理学表现，不典型病理表现的出现可干扰疾病诊断，需要在鉴别诊断时予以重视。

关键词：银屑病回顾性研究临床表现组织病理

Investigation of therapeutic modalities of G719X, an uncommon mutation in the EGFR gene in non-small cell lung cancer

在线全文

学校读者我要写书评

暂无评论

Oncology and Translational Medicine 2019年第2期5卷 91-97页

作者： Hua Zheng Yuan Gao Zan Liu Zhe Qian tongmei zhang Jie Li Hongmei zhang Qunhui Wang Fanbin Hu Baolan Li Department of Medical Oncology Beijing Chest Hospital Capital Medical University

Objective G719 X is the most frequently seen uncommon mutation of the epidermal growth factor receptor(EGFR) gene, which is a point mutation at exon 18 with three common subtypes, G719 A/G719 C/G719 S. This study explored the clinicopathological characteristics of the G719 X mutation and investigated the efficacy of EGFR-tyrosine kinase inhibitor(TKI) treatment and chemotherapy in patients with the G719 X mutation; the survival rate after these different treatment modalities were then analyzed in order to provide evidence for clinical *** Clinical data of 41 patients with the G719 X mutation admitted in the Beijing Chest Hospital, Capital Medical University from September 2014 to July 2018, were collected and the EGFR mutations were detected by amplification refractory mutation system-polymerase chain reaction(ARMS-PCR). The clinicopathological characteristics of the G719 X mutation were analyzed, and the relationship among the G719 X mutation, the efficacy of different treatment modalities, and the progression-free survival(PFS) was analyzed. Results Of the 41 cases, 24(58.5%) were G719 X single mutations and 17(41.5%) were compound mutations, including G719 X/S768 I, G719 X/L861 Q, G719 X/19 del, and G719 X/c-Met compound mutation. The objective response rate(ORR) of first-line EGFR-TKI therapy was 50%(6/12), the disease control rate(DCR) was 83.3%(10/12), and the median PFS(mPFS) was 9 months. After resistance to EGFR-TKI in the previous treatment, the ORR(71.4%, 5/7) and DCR(100%, 7/7) were still high following EGFR-TKIs, by an mPFS of 8 months. The ORR of chemotherapy was 33.3%(2/6), the DCR was 100%(6/6), and the mPFS was 6 months. Conclusion G719 X is an uncommon mutation of the EGFR gene and is sensitive to many EGFR-TKIs. It can be treated with the second-or third-generation EGFR-TKIs after resistance to the first-generation EGFR-TKIs. G719 X mutation also showed favorable effect to chemotherapy.

关键词： lung neoplasms EGFR uncommon mutation G719X target therapy

The expression of vascular endothelial growth factor(VEGF)/endostatin(ES)and VEGF receptor 2(VEGFR2)/ES is associated with NSCLC prognosis

在线全文

学校读者我要写书评

暂无评论

Oncology and Translational Medicine 2021年第4期7卷 149-154页

作者： Yuan Yang Baohua Lu Baolan Li Weiying Li Mei Jiang Wentao Yue Qunhui Wang tongmei zhang General Department of Medicine Beijing Chest HospitalCapital Medical UniversityBeijing 101149China Laboratory of Cell Biotechnology Beijing Tuberculosis and Thoracic Tumor Research InstituteBeijing 101149China

Objective The aim of our study was to detect the expression of angiogenesis inhibitory proteins and angiogenesis promotive proteins in the postoperative tumor tissue of non-small cell lung cancer(NSCLC)*** also investigated the relationship of protein expression with clinical characteristics and *** We examined the expression of vascular endothelial growth factor(VEGF),VEGF receptor 2(VEGFR2),and endostatin(ES)proteins in 255 specimens resected from NSCLC patients,using immune histochemistry(IHC).We then evaluated the relationships between the expression of the three proteins and clinical characteristics such as stage,histological type,differentiation,gender,tobacco use,and *** to the value of VEGF/ES,we divided the cohort into angiogenesis-promoting group A,angiogenesis-inhibiting group A,and balance group *** survival differences in the three groups were evaluated to determine the prognostic value of VEGF/***,we tested the prognostic value of VEGFR2/*** VEGF-positive expression was observed in 93 patients(36.4%).VEGF expression was not correlated with the clinical ***2-positive expression was observed in 103 patients(40.4%).The expression of VEGFR2 was correlated with the clinical stage(χ^(2)=21.414,P=0.045)and histological type(χ^(2)=26.911,P=0.008).ES-positive expression was observed in 140 patients(54.9%).The expression of ES was correlated with the clinical stage(χ^(2)=26.504,P=0.009).When evaluating the prognostic values of VEGF/ES and VEGFR2/ES,the prognosis of the angiogenesis balance group was similar to that of the angiogenesis-inhibiting *** minimum survival time was observed in the angiogenesis-promoting *** VEGF/ES and VEGFR2/ES in resected tumors have prognostic value in postoperative NSCLC *** survival time of the population with predominant angiogenic factors was short.

关键词： non-small cell lung cancer(NSCLC) angiogenesis clinical characteristics prognosis

免疫检查点抑制剂在驱动基因阳性晚期非小细胞肺癌中的应用进展

在线全文

学校读者我要写书评

暂无评论

中国肺癌杂志 2021年第3期24卷 188-195页

作者：张同梅(综述) 李宝兰(审校) 首都医科大学附属北京胸科医院综合科北京市结核病胸部肿瘤研究所北京101149

随着肿瘤精准医学的发展,驱动基因阳性非小细胞肺癌(non-small cell lung cancer,NSCLC)患者接受靶向治疗极大地改善了生存和预后,但不管是哪代靶向药物均不可避免的会经历耐药,患者会面临无靶可用的局面。免疫检查点抑制剂因其特有的... 详细信息

随着肿瘤精准医学的发展,驱动基因阳性非小细胞肺癌(non-small cell lung cancer,NSCLC)患者接受靶向治疗极大地改善了生存和预后,但不管是哪代靶向药物均不可避免的会经历耐药,患者会面临无靶可用的局面。免疫检查点抑制剂因其特有的长拖尾效应,能给部分晚期NSCLC患者带来长生存。越来越多的研究显示免疫治疗同样可为部分驱动基因阳性NSCLC患者带来获益,但免疫治疗介入的时机、治疗方案的选择以及预测生物标记物等问题仍不十分明确,值得进一步探讨。

关键词：肺肿瘤驱动基因免疫检查点抑制剂预测