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Genetically modified pigs with CD163 point mutation are resistant to HP-PRRSV infection

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Zoological Research 2024年第4期45卷 833-844页

作者： Ying Liu Lin Yang Hong-Yong xiang Ming Niu Jia-Cheng Deng Xue-Yuan Li Wen-Jie Hao Hong-Sheng Ou-Yang tong-Yu Liu Xiao-Chun Tang Da-Xin Pang Hong-Ming Yuan State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases Animal Genome Editing Technology Innovation CenterCollege of Animal SciencesJilin UniversityChangchunJilin 130062China Chifeng Best Genetics Technology Co. Ltd.ChifengNei Mongol 024000China Chongqing Jitang Biotechnology Research Institute Co. Ltd.Chongqing 400015China

Porcine reproductive and respiratory syndrome(PRRS)is a globally prevalent contagious disease caused by the positive-strand RNA PRRS virus(PRRSV),resulting in substantial economic losses in the swine *** the CD163 SRCR5 domain,either through deletion or substitution,can eff1ectively confer resistance to PRRSV infection in ***,large fragment modifications in pigs inevitably raise concerns about potential adverse effects on growth *** the impact of genetic modifications on normal physiological functions is a promising direction for developing PRRSV-resistant *** the current study,we identified a specific functional amino acid in CD163 that influences PRRSV *** infection experiments conducted on Marc145 and PK-15CD163 cells illustrated that the mE535G or corresponding pE529G mutations markedly inhibited highly pathogenic PRRSV(HP-PRRSV)proliferation by preventing viral binding and ***,individual viral challenge tests revealed that pigs with the E529G mutation had viral loads two orders of magnitude lower than wild-type(WT)pigs,confirming effective resistance to *** of the physiological indicators and scavenger function of CD163 verified no significant differences between the WT and E529G *** findings suggest that E529G pigs can be used for breeding PRRSV-resistant pigs,providing novel insights into controlling future PRRSV outbreaks.

关键词： PRRSV CD163 Point mutation E529G Pigs

Novel PLCZ1 mutation caused polyspermy during in vitro fertilization

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Asian Journal of Andrology 2024年第4期26卷 389-395页

作者： Ke-Ya tong Wei-Wei Liu Li-Wei Sun Dong-Yun Liu Ye-Zhou xiang Chong Li Lu-Wei Chai Ke Chen Guo-Ning Huang Jing-Yu Li Chongqing Key Laboratory of Human Embryo Engineering Center for Reproductive MedicineWomen and Children’s Hospital of Chongqing Medical UniversityChongqing 400012China Chongqing Clinical Research Center for Reproductive Medicine Center for Reproductive MedicineChongqing Health Center for Women and ChildrenChongqing 400012China.

Failure of oocyte activation,including polyspermy and defects in pronuclear(PN)formation,triggers early embryonic developmental *** studies have shown that phospholipase C zeta 1(PLCZ1)mutations cause failure of PN formation following intracytoplasmic sperm injection(ICSI);however,whether PLCZ1 mutation is associated with polyspermy during in vitro fertilization(IVF)remains ***-exome sequencing(WES)was performed to identify candidate mutations in couples with primary *** sequencing was used to validate the *** PLCZ1-mutated sperm were injected into human and mouse oocytes to explore whether PN formation was *** oocyte activation(AOA)after ICSI was performed to overcome the failure of oocyte *** identified three PLCZ1 mutations in three patients who experienced polyspermy during IVF cycles,including a novel missense mutation c.1154C>T,*** formation failure was observed during the ICSI ***,injection of multiple PLCZ1-mutated sperm induced PN formation,suggesting that the Ca2+oscillations induced by the sperm exceeded the necessary threshold for PN *** after ICSI enabled normal fertilization,and all patients achieved successful *** findings expand the mutational spectrum of PLCZ1 and suggest an important role for PLCZ1 in terms of blocking ***,this study may benefit genetic diagnoses in cases of abnormal fertilization and provide potential appropriate therapeutic measures for these patients with sperm-derived polyspermy.

关键词： AOA PLCZ1 polyspermy pronuclear formation

融合加速度与计算机视觉实测车致响应的梁桥损伤识别方法

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中国公路学报 2024年第2期37卷 152-167页

作者：吴桐唐亮周丰力张玉丹周志祥重庆交通大学土木工程学院重庆400074 深圳大学土木与交通工程学院广东深圳518060

基于结构振型相关参数的损伤识别方法对振型的空间分辨率具有较高要求。为了改进计算机视觉技术在桥梁结构全场振型提取和损伤识别方面的不足,同时充分利用SHM系统各类传感器采集的丰富数据,提出了一种基于计算机视觉和有限数量加速度... 详细信息

基于结构振型相关参数的损伤识别方法对振型的空间分辨率具有较高要求。为了改进计算机视觉技术在桥梁结构全场振型提取和损伤识别方面的不足,同时充分利用SHM系统各类传感器采集的丰富数据,提出了一种基于计算机视觉和有限数量加速度传感器实测桥梁在移动荷载作用下响应的损伤识别方法。首先,通过理论推导研究了损伤前后任意边界条件单跨梁在移动集中力作用下的位移响应,并从理论角度分析了局部刚度损伤引发的结构位移响应和振型改变。在此基础上,提出了一种通过测量少量测点的计算机视觉位移和加速度响应,提取可表征结构真实状态的多阶高空间分辨率振型的方法,所得振型的空间分辨率取决于移动荷载速度和位移采样频率。根据所提取振型的特点,提出了一种多阶全场模态曲率面积差加权融合的结构损伤识别参数,该参数可以直接利用损伤前后该单元前后节点的模态转角求得。为了验证所提出的方法,开展了实验室简支梁模型在不同速度和大小的移动荷载作用下的多工况损伤识别试验。试验结果表明,所提出的方法可以通过计算机视觉和有限数量的加速度传感器实现模型梁全场振型提取,且该振型与测点加速度模态分析得出的结果相符,空间分辨率可达模型梁跨径的1/6 000~1/3 000。此外,所提出的损伤识别指标在不同工况下能够准确地识别损伤位置,即使损伤前后的移动荷载大小和速度存在差异,该指标也能较准确地定位损伤。

关键词：桥梁工程损伤识别计算机视觉模态曲率全场振型

基于RCH-UNet的新疆密植棉花图像快速分割及产量预测

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农业工程学报 2024年第7期40卷 230-239页

作者：刘祥田敏梁金艳石河子大学机械电气工程学院石河子832003

针对复杂棉田环境下传统图像分割技术存在分割精度低、实时性弱和鲁棒性差等问题,该研究以脱叶期新疆密植棉花为对象,结合低空无人机遥感平台,提出一种RCH-UNet(resnet coordinate hardswish UNet)棉花产量快速预测模型。将UNet中传统的... 详细信息

针对复杂棉田环境下传统图像分割技术存在分割精度低、实时性弱和鲁棒性差等问题,该研究以脱叶期新疆密植棉花为对象,结合低空无人机遥感平台,提出一种RCH-UNet(resnet coordinate hardswish UNet)棉花产量快速预测模型。将UNet中传统的CBR(convolution batch normalization ReLU)下采样模块替换为ResNet50,同时将CA(coordinate attention)注意力机制和hardswish激活函数引入UNet,以提高图像特征的提取能力,增强图像分割效果。基于无人机采集的棉花图像数据集评估RCH-UNet模型性能。试验结果表明,在该研究构建的棉花图像数据集上,RCH-UNet模型的棉花分割交并比达到92.79%,像素准确率达到96.22%,精确率为96.30%,与原始U-Net、PSPNet和DeepLabv3相比,像素准确率分别提高了9.85、17.67、6.31个百分点。通过RCH-UNet提取棉花像素比和灰度共生矩阵提取纹理特征,结合岭回归分析构建多因素棉花产量预测模型,模型的R^(2)为0.92,预测产量与实际产量平均绝对百分比误差为9.254%。研究结果可为新疆密植棉花产量预测提供技术支持。

关键词：深度学习图像处理语义分割 UNet 自注意力机制密植棉花产量预测

Data-driven polarimetric imaging: a review

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Opto-Electronic Science 2024年第2期3卷 1-44页

作者： Kui Yang Fei Liu Shiyang liang Meng xiang Pingli Han Jinpeng Liu Xue Dong Yi Wei Bingjian Wang Koichi Shimizu Xiaopeng Shao School of Optoelectronic Engineering Xidian UniversityXi’an 710071China School of Physics Xidian UniversityXi’an 710071China Graduate School of Information Production and SystemsWaseda UniversityKitakyushu 808-0135Japan Department of Mechanical Engineering Massachusetts Institute of TechnologyCambridgeMA 02139USA Hangzhou Institute of Technology Xidian UniversityHangzhou 311200China

This study reviews the recent advances in data-driven polarimetric imaging technologies based on a wide range of practical *** widespread international research and activity in polarimetric imaging techniques demonstrate their broad applications and *** information is increasingly incorporated into convolutional neural networks(CNN)as a supplemental feature of objects to improve performance in computer vision task *** imaging and deep learning can extract abundant information to address various ***,this article briefly reviews recent developments in data-driven polarimetric imaging,including polarimetric descattering,3D imaging,reflection removal,target detection,and biomedical ***,we synthetically analyze the input,datasets,and loss functions and list the existing datasets and loss functions with an evaluation of their advantages and *** also highlight the significance of data-driven polarimetric imaging in future research and development.

关键词： deep learning polarimetric imaging image processing

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四桨船舶尾流场特性数值预报研究

船舶力学 2024年第8期28卷 1187-1199页

作者：李想贾伟梁宁海南大学海洋科学与工程学院海口570228 哈尔滨工程大学船舶工程学院哈尔滨150001

四桨船尾部附体的复杂性及内外桨空间布置位置的差异性会导致内外桨载荷分配不均衡。针对上述问题,本文采用CFD方法对四桨船舶的粘性尾流场进行数值模拟研究,从船体周围流场分布、湍流特性等方面详细分析内外桨盘面伴流场差异产生的原因... 详细信息

四桨船尾部附体的复杂性及内外桨空间布置位置的差异性会导致内外桨载荷分配不均衡。针对上述问题,本文采用CFD方法对四桨船舶的粘性尾流场进行数值模拟研究,从船体周围流场分布、湍流特性等方面详细分析内外桨盘面伴流场差异产生的原因,并与试验结果进行对比分析。研究结果表明,内桨处于船尾湍流区中,受船体边界层影响更甚,这使得内桨桨盘面轴向速度小于外桨盘面,进而导致内外桨负荷分配不均匀;外桨盘面流动为船体边界层流动及湍流尾流区的混合流动,这使得外桨伴流场更不均匀。上述研究可为四桨船舶设计及尾部布局优化提供基础和支撑。

关键词：四桨船舶尾流场边界层流动流场均匀度数值模拟

GRB 210610B:The Internal and External Plateau as Evidence for the Delayed Outflow of Magnetar

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Research in Astronomy and Astrophysics 2024年第7期24卷 170-180页

作者： Yi-Ning Wei xiang-Gao Wang Da-Bin Lin Wei-Kang Zheng liang-Jun Chen Sheng-Yu Yan Shuang-Xi Yi Qi Wang Zi-Min Zhou Hui-Ya Liu En-Wei liang Guangxi Key Laboratory for Relativistic Astrophysics School of Physical Science and TechnologyGuangxi UniversityNanning 530004China GXU-NAOC Center for Astrophysics and Space Sciences Nanning 530004China Department of Astronomy University of CaliforniaBerkeleyCA 94720-3411USA Department of Physics and Tsinghua Center for Astrophysics(THCA) Tsinghua UniversityBeijing 100084China School of Physics and Physical Engineering Qufu Normal UniversityQufu 273165China

After launching a jet,outflows of magnetar were used to account for the achromatic plateau of afterglow and the early X-ray flux plateau known as“internal plateau”.The lack of detecting magnetic dipole emission together with the energy injection feature in a single observation poses confusion until the long gamma-ray burst(GRB)210610B is *** 210610B is presented with an optical bump following an early X-ray plateau during the afterglow *** plateau followed by a steep decline flux overlays in the steadily decaying X-ray flux with indexα_(X,1)~2.06,indicating an internal origin and that can be fitted by the spin-down luminosity law with the initial plateau luminosity log_(10)L_(X)~48.29 erg s~(-1)and the characteristic spin-down timescale T~2818 s.A subsequent bump begins at~4000 s in the R band with a rising indexα_(R,1)~-0.30 and peaks at~14125 s,after which a decay indexα_(R,2)~0.87 and finally transiting to a steep decay withα_(R,3)~1.77 achieve the closure relation of the external shock for the normal decay phase as well as the magnetar spin-down energy injection phase,provided that the average value of the photon indexΓ_γ=1.80 derived from the spectral energy distributions(SEDs)between the X-ray and optical *** closure relation also works for the late X-ray *** to the traditional picture of GRB,the outflow powers the early X-ray plateau by dissipating energy internally and collides with the leading decelerating blast burst as time goes on,which could interpret the exotic feature of GRB *** carry out a Markov Chain Monte Carlo simulation and obtain a set of best parameters:■.The artificial light curve can fit the afterglow data *** that,we estimated the average Lorentz factor and the X-ray radiation efficiency of the later ejecta are 35%and 0.13%,respectively.

关键词： (stars)gamma-ray burst individual(GRB 210610B)-(stars)gamma-ray burst general-stars jets

Advancements and challenges in bionic joint lubrication biomaterials for sports medicine

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BIOSURFACE AND BIOTRIBOLOGY 2024年第3期10卷 89-105页

作者： xiang, Lei Wang, Zhen Cui, Wenguo Shanghai Jiao tong Univ Shanghai Inst Traumatol & Orthopaed Shanghai Key Lab Prevent & Treatment Bone & Joint Dept OrthopaedRuijin HospSch Med Shanghai Peoples R China

Bionic lubricant materials are a class of materials inspired by natural organisms and offer excellent lubrication properties and biocompatibility. In the field of sports medicine, their application opens up new possibilities for the prevention and treatment of sports-related diseases. The authors will introduce the existing theoretical models of friction in the locomotor system, the characteristics and advantages of biomimetic lubrication materials and discuss in depth their applications in the field of sports medicine. The development of bionic lubrication materials opens up unprecedented opportunities for sports medicine to provide more effective and long-lasting treatment options for patients.

关键词： biointerface bio-lubricant biomaterials biomedical application medicine FLEXOR TENDON REPAIR SYNOVIAL-FLUID BOUNDARY LUBRICATION SURFACE MODIFICATION ARTICULAR-CARTILAGE MOLECULAR-STRUCTURE GLIDING RESISTANCE OSTEOARTHRITIS LUBRICIN PREVENTION

XELOX(capecitabine plus oxaliplatin)plus bevacizumab(anti-VEGF-A antibody)with or without adoptive cell immunotherapy in the treatment of patients with previously untreated metastatic colorectal cancer:a multicenter,open-label,randomized,controlled,phase 3 trial

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Signal Transduction and Targeted Therapy 2024年第5期9卷 2104-2113页

作者： Qiu-Zhong Pan Jing-Jing Zhao liang Liu Dong-Sheng Zhang Li-Ping Wang Wen-Wei Hu De-Sheng Weng xiang Xu Yi-Zhuo Li Yan Tang Wei-Hong Zhang Jie-Yao Li Xiao Zheng Qi-Jing Wang Yong-Qiang Li tong xiang Li Zhou Shuang-Ning Yang Chen Wu Rong-Xing Huang Jia He Wei-Jiao Du Lu-Jun Chen Yue-Na Wu Bin Xu Qiong Shen Yi Zhang Jing-Ting Jiang Xiu-Bao Ren Jian-Chuan Xia State Key Laboratory of Oncology in South China Guangdong Provincial Clinical Research Center for CancerCollaborative Innovation Center for Cancer MedicineSun Yat-Sen University Cancer CenterGuangzhouGuangdong 510060PR China Department of Biotherapy Sun Yat-Sen University Cancer CenterGuangzhouGuangdong 510060PR China Tianjin Medical University Cancer Institute and Hospital National Clinical Research Center for CancerTianjin 300060PR China Key Laboratory of Cancer Immunology and Biotherapy Tianjin 300060PR China Department of Biotherapy/Immunology Tianjin Medical University Cancer Institute and HospitalTianjin 300060PR China Department of Medical Oncology Sun Yat-sen University Cancer CenterGuangzhou 510060PR China Department of Oncology the First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052PR China Department of Tumor Biological Treatment the Third Affiliated Hospital of Soochow UniversityChangzhouJiangsu 213003PR China Jiangsu Engineering Research Center for Tumor Immunotherapy ChangzhouJiangsu 213003PR China Institute for Cell Therapy of Soochow University ChangzhouJiangsu 213003PR China Department of Stem Cell&Regenerative Medicine State Key Laboratory of TraumaBurn and Combined InjuryDaping HospitalArmy Medical UniversityChongqing 400042PR China Imaging Diagnosis and Interventional Center Sun Yat-sen University Cancer CenterGuangzhouGuangdong 510060PR China

Fluoropyrimidine-based combination chemotherapy plus targeted therapy is the standard initial treatment for unresectable metastatic colorectal cancer(mCRC),but the prognosis remains *** phase 3 trial(***:NCT03950154)assessed the efficacy and adverse events(AEs)of the combination of PD-1 blockade-activated DC-CIK(PD1-T)cells with XELOX plus bevacizumab as a first-line therapy in patients with mCRC.A total of 202 participants were enrolled and randomly assigned in a 1:1 ratio to receive either first-line XELOX plus bevacizumab(the control group,n=102)or the same regimen plus autologous PD1-T cell immunotherapy(the immunotherapy group,n=100)every 21 days for up to 6 cycles,followed by maintenance treatment with capecitabine and *** main endpoint of the trial was progression-free survival(PFS).The median follow-up was 19.5 *** PFS was 14.8 months(95%CI,11.6-18.0)for the immunotherapy group compared with 9.9 months(8.0-11.8)for the control group(hazard ratio[HR],0.60[95%CI,0.40-0.88];p=0.009).Median overall survival(OS)was not reached for the immunotherapy group and 25.6 months(95%CI,18.3-32.8)for the control group(HR,0.57[95%CI,0.33-0.98];p=0.043).Grade 3 or higher AEs occurred in 20.0%of patients in the immunotherapy group and 23.5%in the control groups,with no toxicity-associated deaths *** addition of PD1-T cells to first-line XELOX plus bevacizumab demonstrates significant clinical improvement of PFS and OS with well tolerability in patients with previously untreated mCRC.

关键词： PD1 immunotherapy bevacizumab