检索结果-南通市图书馆

动脉粥样硬化细胞模型建立及细胞内铁代谢紊乱初步研究

在线全文

学校读者我要写书评

暂无评论

介入放射学杂志 2017年第12期26卷 1110-1113页

作者：徐强周瑜乔彤江苏大学附属武进医院介入血管科江苏常州213002 南京大学医学院附属鼓楼医院血管外科

目的建立动脉粥样硬化(AS)细胞模型,研究铁代谢与AS关系及AS斑块中巨噬细胞铁代谢紊乱的分子机制,为临床干预铁代谢及防治AS提供理论依据。方法采用RAW264.7细胞,以氧化型低密度脂蛋白(ox-LDL)诱导制备泡沫细胞,油红O染色并通过酶联免... 详细信息

目的建立动脉粥样硬化(AS)细胞模型,研究铁代谢与AS关系及AS斑块中巨噬细胞铁代谢紊乱的分子机制,为临床干预铁代谢及防治AS提供理论依据。方法采用RAW264.7细胞,以氧化型低密度脂蛋白(ox-LDL)诱导制备泡沫细胞,油红O染色并通过酶联免疫法检测胞内脂质(总胆固醇、游离胆固醇)验证AS泡沫细胞模型建立。免疫印迹法检测铁代谢相关铁蛋白(FT)、膜铁转运蛋白(FPN)1,免疫组化、免疫荧光法检测和定位巨噬细胞中FPN1表达。结果 ox-LDL诱导制备的细胞质内有大量红色脂质颗粒,较多脂质融合成大油滴状,符合泡沫细胞形态特征;泡沫细胞内有大量脂质堆积,胆固醇酯(CE)占比明显高于正常细胞(P<0.05);与正常对照组相比,泡沫细胞内FT含量明显高于正常细胞,FPN1含量增加且主要存在于细胞质内。结论泡沫细胞中铁代谢紊乱,细胞内大量聚集。FPN1非细胞膜定位阻止铁从巨噬细胞中有效排出并加重泡沫细胞中铁累积,可能加剧AS斑块形成和发展。

关键词：动脉粥样硬化泡沫细胞膜铁转运蛋白铁蛋白

Osteomodulin downregulation is associated with osteoarthritis development

在线全文

学校读者我要写书评

暂无评论

Bone Research 2023年第4期11卷 721-736页

作者： Jérémie Zappia qiao tong Renée Van der Cruyssen Frederique MFCornelis Cécile Lambert Tiago Pinto Coelho Juliane Grisart Erika Kague Rik JLories Marc Muller Dirk Elewaut Chrissy LHammond Christelle Sanchez Yves Henrotin MusculoSKeletal Innovative Research Lab Center for Interdisciplinary Research on MedicinesUniversitéde LiègeLiègeBelgium School of Physiology Pharmacologyand NeuroscienceUniversity of BristolBristolUK Department of Biomedical Molecular Biology Ghent UniversityGhentBelgium Laboratory for Molecular Immunology and Inflammation Department of RheumatologyGhent University HospitalGhentBelgium Laboratory of Tissue Homeostasis and Disease Skeletal Biology and Engineering Research CenterDepartment of Development and RegenerationKU LeuvenLeuvenBelgium Cardiovascular Sciences Groupe Interdisciplinaire de Génoprotéomique AppliquéeUniversitéde LiègeLiègeBelgium Division of Nephrology CHU of LiègeUniversitéde LiègeLiègeBelgium Artialis SA Tour GIGACHU Sart-TilmanLiègeBelgium Division of Rheumatology University Hospitals LeuvenLeuvenBelgium Laboratoire d’Organogenèse et Régénération Groupe Interdisciplinaire de Génoprotéomique AppliquéeUniversitéde LiègeLiègeBelgium Physical Therapy and Rehabilitation Department Princess Paola HospitalVivaliaMarche-en-FamenneBelgium

Abnormal subchondral bone remodeling leading to sclerosis is a main feature of osteoarthritis(OA), and osteomodulin(OMD), a proteoglycan involved in extracellular matrix mineralization, is associated with the sclerotic phenotype. However, the functions of OMD remain poorly understood, specifically in vivo. We used Omd knockout and overexpressing male mice and mutant zebrafish to study its roles in bone and cartilage metabolism and in the development of OA. The expression of Omd is deeply correlated with bone and cartilage microarchitectures affecting the bone volume and the onset of subchondral bone sclerosis and spontaneous cartilage lesions. Mechanistically, OMD binds to RANKL and inhibits osteoclastogenesis, thus controlling the balance of bone remodeling. In conclusion, OMD is a key factor in subchondral bone sclerosis associated with OA. It participates in bone and cartilage homeostasis by acting on the regulation of osteoclastogenesis. Targeting OMD may be a promising new and personalized approach for OA.

关键词： metabolism conclusion homeostasis

Graphene binding on black phosphorus enables high on/off ratios and mobility

在线全文

学校读者我要写书评

暂无评论

National Science Review 2024年第2期11卷 188-195页

作者： Fanrong Lin Zhonghan Cao Feiping Xiao Jiawei Liu Jiabin qiao Minmin Xue Zhili Hu Ying Liu Huan Lu Zhuhua Zhang Jens Martin Qingjun tong Wanlin Guo Yanpeng Liu Key Laboratory for Intelligent Nano Materials and Devices of Ministry of Education State Key Laboratory of Mechanics and Control of Mechanical Structures and Institute for Frontier Science Nanjing University of Aeronautics and Astronautics Centre for Advanced 2D Materials National University of Singapore School of Physics and Electronics Hunan University Centre for Quantum Physics Key Laboratory of Advanced Optoelectronic Quantum Architecture and MeasurementSchool of PhysicsBeijing Institute of Technology Leibniz Institute für Kristallzüchtung

Graphene is one of the most promising candidates for integrated circuits due to its robustness against short-channel effects, inherent high carrier mobility and desired gapless nature for Ohmic contact, but it is difficult to achieve satisfactory on/off ratios even at the expense of its carrier mobility, limiting its device applications. Here, we present a strategy to realize high back-gate switching ratios in a graphene monolayer with well-maintained high mobility by forming a vertical heterostructure with a black phosphorus multi-layer. By local current annealing, strain is introduced within an established area of the graphene, which forms a reflective interface with the rest of the strain-free area and thus generates a robust off-state via local current depletion. Applying a positive back-gate voltage to the heterostructure can keep the black phosphorus insulating, while a negative back-gate voltage changes the black phosphorus to be conductive because of hole accumulation. Then, a parallel channel is activated within the strain-free graphene area by edge-contacted electrodes, thereby largely inheriting the intrinsic carrier mobility of graphene in the on-state. As a result, the device can provide an on/off voltage ratio of >10～3 as well as a mobility of ～8000 cm2V-1s-1at room temperature, meeting the low-power criterion suggested by the International Roadmap for Devices and Systems.

关键词： graphene on/off ratio mobility black phosphorus reflective interface

The spectrum and stability of travelling pulses in a coupled FitzHugh-Nagumo equation

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Science China Mathematics 2024年第5期67卷 975-1010页

作者： Qi qiao Xiang Zhang School of Mathematical Sciences Shanghai Jiao Tong UniversityShanghai 200240China Key Laboratory of Scientific and Engineering Computing(Ministry of Education) and Shanghai Frontier Science Center of Modern Analysis(CMA-Shanghai)Shanghai Jiao Tong UniversityShanghai 200240China

For a coupled slow-fast FitzHugh-Nagumo(FHN)equation derived from a reaction-diffusionmechanics(RDM)model,Holzer et al.(2013)studied the existence and stability of the travelling pulse,which consists of two fast orbit arcs and two slow ones,where one fast segment passes the unique fold point with algebraic decreasing and two slow ones follow normally hyperbolic critical curve *** and Zhang(2021)obtained the existence of the travelling pulse,whose two fast orbit arcs both exponentially decrease,and one of the slow orbit arcs could be normally hyperbolic or not at the ***,we characterize both the nonlinear and spectral stability of this travelling pulse.

关键词： coupled FitzHugh-Nagumo equation singular perturbation travelling pulse spectrum nonlinear stability spectral stability

在线全文

学校读者我要写书评

暂无评论

主动脉夹层实验模型的研究进展

中华心血管病杂志 2022年第8期50卷 839-844页

作者：季润乔彤赵晓棋南京中医药大学鼓楼临床医学院血管外科南京210008 南京大学医学院附属鼓楼医院血管外科南京210008

主动脉夹层(AD)是一种发病突然、进展迅速且致死率高的大血管疾病,目前对AD的发病机制与治疗手段的认识仍有待进一步提高。AD实验模型主要分为在体模型和离体模型两大类:在体模型主要通过化学物质或药物诱导、转基因方式建模和手术(开... 详细信息

主动脉夹层(AD)是一种发病突然、进展迅速且致死率高的大血管疾病,目前对AD的发病机制与治疗手段的认识仍有待进一步提高。AD实验模型主要分为在体模型和离体模型两大类:在体模型主要通过化学物质或药物诱导、转基因方式建模和手术(开放手术或腔内手术)诱导,离体模型是指在体外模拟AD的形成。本文就目前报道的AD实验模型的各种建模方式作简要综述。

关键词：主动脉动脉瘤,夹层实验模型

Integrative analysis of the transcriptome and metabolome reveals the importance of hepatokine FGF21 in liver aging

在线全文

学校读者我要写书评

暂无评论

Genes & Diseases 2024年第5期11卷 407-427页

作者： Wenchao Wang Junjie Qian Mingge Shang Yiting qiao Jiacheng Huang Xinxin Gao Zhou Ye Xinyu tong Kangdi Xu Xiang Li Zhengtao Liu Lin Zhou Shusen Zheng Division of Hepatobiliary and Pancreatic Surgery Department of SurgeryFirst Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhouZhejiang 310003China NHC Key Laboratory of Combined Multi-organ Transplantation HangzhouZhejiang 310003China Key Laboratory of the Diagnosis and Treatment of Organ Transplantation Research Unit of Collaborative Diagnosis and Treatment for Hepatobiliary and Pancreatic CancerChinese Academy of Medical Sciences(2019RU019)HangzhouZhejiang 310003China Key Laboratory of Organ Transplantation Research Center for Diagnosis and Treatment of Hepatobiliary DiseasesHangzhouZhejiang 310003China Shulan International Medical College Zhejiang Shuren UniversityHangzhouZhejiang 310015China Shulan(Hangzhou)Hospital Affiliated to Zhejiang Shuren University Shulan International Medical College HangzhouZhejiang 310000China

Aging is a contributor to liver ***,the concept of liver aging has become prominent and has attracted considerable interest,but its underlying mechanism remains poorly *** our study,the internal mechanism of liver aging was explored via multi-omics analysis and molecular experiments to support future targeted *** aged rat liver model was established with D-galactose,and two other senescent hepatocyte models were established by treating HepG2 cells with D-galactose and *** then performed transcriptomic and metabolomic assays of the aged liver model and transcriptome analyses of the senescent hepatocyte *** livers,genes related to peroxisomes,fatty acid elongation,and fatty acid degradation exhibited down-regulated expression with aging,and the hepatokine Fgf21 expression was positively correlated with the down-regulation of these *** senescent hepatocytes,similar to the results found in aged livers,FGF21 expression was also ***,the expressions of cell cycle-related genes were significantly down-regulated,and the down-regulated gene E2F8 was the key cell cycle-regulating transcription *** then validated that FGF21 overexpression can protect against liver aging and that FGF21 can attenuate the declines in the antioxidant and regenerative capacities in the aging *** successfully validated the results from cellular and animal experiments using human liver and blood *** study indicated that FGF21 is an important target for inhibiting liver aging and suggested that pharmacological prevention of the reduction in FGF21 expression due to aging may be used to treat liver aging-related diseases.

关键词： Antioxidant capability FGF21 Liver aging Liver regeneration Multiomics analysis

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

曲张病变大隐静脉第一对瓣膜的病理改变

江苏医药 2011年第13期37卷 1546-1548,F0002页

作者：王鑫乔彤南京医科大学鼓楼临床医学院血管外科 210008

目的探讨曲张病变大隐静脉第一对瓣膜的病理改变。方法大隐静脉曲张(A)组24例,正常对照(C)组3例,分别采用HE、Sirius Red染色及CD31、-αSMA免疫组织化学法检测大隐静脉第一对静脉瓣在曲张病变中的病理改变。结果与C组比较,A组静脉瓣膜... 详细信息

目的探讨曲张病变大隐静脉第一对瓣膜的病理改变。方法大隐静脉曲张(A)组24例,正常对照(C)组3例,分别采用HE、Sirius Red染色及CD31、-αSMA免疫组织化学法检测大隐静脉第一对静脉瓣在曲张病变中的病理改变。结果与C组比较,A组静脉瓣膜延伸变长、扭曲变形,伴胶原纤维增生,瓣膜两侧内皮细胞表达CD31不一致(P<0.05),并异常高表达-αSMA(P<0.05)。结论曲张大隐静脉中瓣膜的形态、结构、内皮均发生了明显病理改变,提示静脉瓣膜的这些改变在下肢静脉曲张的发病机制中起重要作用。

关键词：大隐静脉曲张

A contradictory phenomenon of thicken pericardium and cardiac compression without inferior vena cava dilation:sign of IVC escape

在线全文

学校读者我要写书评

暂无评论

Journal of Geriatric Cardiology 2024年第5期21卷 583-587页

作者： Tie-Nan CHEN Shuang ZHAO Shuai qiao Yong-Yong HAN Qing LIU Chang-Le LIU Guang-Ping LI tong LIU Hua-Ying FU Department of Cardiovascular Surgery Second Hospital of Tianjin Medical UniversityTianjinChina Department of Cardiology Tianjin Key Laboratory of Ionic-Molecular Function of Cardiovascular DiseaseTianjin Institute of Cardi-ologyThe Second Hospital of Tianjin Medical UniversityTianjinChina Department of Oncology Second Hospital of Tianjin Medical UniversityTianjinPeople's Republic of China

A 77-year-old female presented with shortness of breath and tightness of chest was *** past medical history included hypertension and she has been taking nifedipine *** years before,she was diagnosed with pericardial effusion(Figure 1)and had pericardiocentesis *** physical examination,her blood pressure was 151/100 mm-Hg and her pulse rate was 91 beats/min.

关键词： admitted cardiac thick

对高强度功能训练商业案例的科学审视——CrossFit体系介绍与研究综述

在线全文

学校读者我要写书评

暂无评论

中国体育科技 2019年第5期55卷 72-80页

作者：乔通黎涌明上海体育学院

高强度功能训练(high-intensity functional training,HIFT)是近年来兴起的一种热门健身方式,CrossFit(CF)是世界上发展最快的HIFT的商业载体之一。本文回顾了CF的起源与发展,介绍并审视了CF经营和训练体系,综述了CF训练导致的伤病以及C... 详细信息

高强度功能训练(high-intensity functional training,HIFT)是近年来兴起的一种热门健身方式,CrossFit(CF)是世界上发展最快的HIFT的商业载体之一。本文回顾了CF的起源与发展,介绍并审视了CF经营和训练体系,综述了CF训练导致的伤病以及CF训练对生理和心理的影响。CF在十几年间风靡全球与其以教练认证、加盟和赛事为核心的商业体系和以全面强健理念、功能性动作和高强度为核心的训练体系密切相关,其在商业模式和训练体系上存在的不足则引发了对该训练的安全性和科学性的担忧。有限的研究发现,CF训练的损伤率与其他非接触性运动相当,CF训练相关的生理学研究较少,现有研究中基于流行或专门制定的每日训练计划可否代表CF训练有待商榷,CF训练对人体生理和心理特征的影响也有待更多的研究。CF在我国的发展面临诸多本土化问题。

关键词：高强度功能训练商业体系训练体系本土化