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Butyrate stimulates IL-32α expression in human intestinal epithelial cell lines

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World Journal of Gastroenterology 2010年第19期16卷 2355-2361页

作者： Ayako Kobori Shigeki Bamba Hirotsugu Imaeda Hiromitsu Ban Tomoyuki Tsujikawa Yasuharu Saito Yoshihide Fujiyama Akira Andoh Division of Gastroenterology Department of Internal Medicine Shiga University of Medical Science Seta Tukinowa Otsu 520-2192 Japan Division of Mucosal Immunology Graduate School of Medicine Shiga University of Medical Science Seta Tukinowa Otsu 520-2192 Japan

AIM: To investigate the effects of butyrate on interleukin (IL)-32α expression in epithelial cell lines. METHODS: The human intestinal epithelial cell lines HT-29, SW480, and T84 were used. Intracellular IL- 32α was determined by Western blotting analyses. IL- 32α mRNA expression was analyzed by real-time poly-merase chain reaction. RESULTS: Acetate and propionate had no effects on IL-32α mRNA expression. Butyrate significantly enhanced IL-32α expression in all cell lines. Butyrate also up-regulated IL-1β-induced IL-32α mRNA expression. Butyrate did not modulate the activation of phosphatidylinositol 3-kinase (PI3K), a mediator of IL- 32α expression. Like butyrate, trichostatin A, a histone deacetylase inhibitor, also enhanced IL-1β-induced IL- 32α mRNA expression.CONCLUSION: Butyrate stimulated IL-32α expression in epithelial cell lines. An epigenetic mechanism, such as histone hyperacetylation, might be involved in the action of butyrate on IL-32α expression.

关键词： Cytokine Histone acetylation Inflammatory bowel disease

Application of novel magnified single balloon enteroscopy for a patient with Cronkhite-Canada syndrome

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World Journal of Gastroenterology 2017年第22期23卷 4121-4126页

作者： Masaki Murata Shigeki Bamba Kenichiro Takahashi Hirotsugu Imaeda Atsushi Nishida Osamu Inatomi Tomoyuki Tsujikawa Ryoji Kushima Mitsushige Sugimoto Akira Andoh Department of Medicine Shiga University of Medical Science Comprehensive Internal Medicine Shiga University of Medical ScienceSeta-Tsukinowa Department of Clinical Laboratory Medicine Shiga University of Medical Science Division of Digestive endoscopy Shiga University of Medical Science

We present a case of Cronkhite-Canada syndrome(CCS) in which the entire intestine was observed using a prototype of magnifying single-balloon enteroscope(SIF Y-0007, Olympus). CCS is a rare, non-familial gastrointestinal polyposis with ectodermal abnormalities. To our knowledge, this is the first report showing magnified intestinal lesions of CCS. A 73-year-old female visited our hospital with complaints of diarrhea and dysgeusia. The blood test showed mild anemia and hypoalbuminemia. The esophagogastroduodenoscopy and colonoscopy revealed diffuse and reddened sessile to semi-pedunculated polyps, resulting in the diagnosis of CCS. In addition to the findings of conventional balloon-assisted enteroscopy or capsule endoscopy, magnifying observation revealed tiny granular structures, non-uniformity of the villus, irregular caliber of the loop-like capillaries, scattered white spots in the villous tip, and patchy redness of the villus. Histologically, the scattered white spots and patchy redness of the villus reflect lymphangiectasia and bleeding to interstitium, respectively.

关键词：帮助汽球的 enteroscopy 放大内视镜检查法缩小乐队成像小肠

FR167653,a p38 mitogen-activated protein kinase inhibitor,aggravates experimental colitis in mice

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World Journal of Gastroenterology 2008年第38期14卷 5851-5856页

作者： Takashi Nishimura Akira Andoh Atsushi Nishida Makoto Shioya Yuhsuke Koizumi Tomoyuki Tsujikawa Yoshihide Fujiyama Department of Medicine Shiga University of Medical Science Seta TsukinowaOtsu 520-2192 Japan

AIM: To investigate the effects of FR167653 on the development of dextran sulfate sodium (DSS)-induced colitis in mice. METHODS: BALB/c mice were fed rodent chow containing 3.5% (wt/wt) DSS. The recipient mice underwent intra-peritoneal injection of vehicles or FR167653 (30 mg/kg per day). The mice were sacrificed on day 14, and the degree of colitis was assessed. Immunohistochemical analyses for CD4+ T cell and F4/80+ macrophage infiltration were also performed. Mucosal cytokine expression was analyzed by RT-PCR. RESULTS: The body weight loss was more apparent in the FR167653-treated DSS mice than in the vehicle- treated DSS mice. The colon length was shorter in the FR167653-treated DSS mice than in the vehicle-treated DSS mice. Disease activity index and histological colitis score were significantly higher in FR167653- than in vehicle-treated DSS animals. Microscopically, mucosal edema, cellular infiltration (CD4 T cells and F4/80 macrophages), and the disruption of the epithelium were much more severe in FR167653-treated mice than in controls. Mucosal mRNA expression for interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) were found to be markedly reduced in FR167653-treated DSS mice. CONCLUSION: Treatment with FR167653 aggravated DSS colitis in mice. This effect was accompanied by a reduction of mucosal IL-1β and TNF-α expression, suggesting a role of p38 mitogen-activated protein kinase (MAPK)-mediated proinflammatory cytokine induction in host defense mechanisms.

关键词：结肠炎肿瘤坏死因子α 肠炎活性蛋白激酶抑制剂

Usefulness of bispectral monitoring of conscious sedation during endoscopic mucosal dissection

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World Journal of Gastroenterology 2009年第5期15卷 595-598页

作者： Kazunori Hata Akira Andoh Kiyoyuki Hayafuji Atsuhiro Ogawa Tamio Nakahara Tomoyuki Tsujikawa Yoshihide Fujiyama Yasuharu Saito Department of Medicine Shiga University of Medical Science Seta-Tukinowa Otsu 520-2192 Japan Division of Endoscopy Shiga University of Medical Science Seta-Tukinowa Otsu 520-2192 Japan

AIM: To assess the usefulness of bispectral index (BIS) monitoring in order to carry out endoscopic submucosal dissection (ESD) safely and with patients' satisfaction. METHODS: Three hundred sixty-six patients with an early-stage neoplasm of the digestive tract were enrolled. The BIS monitor (A-1050: Aspect medical systems/NIHON KOHDEN, Tokyo, Japan) was used. The appropriate sedative condition was set at 55 to 75 BIS levels (BIS value) during the endoscopic procedures. RESULTS: Among 366 cases, 13 were accompanied by adverse events during and/or after ESD. All episodes occurred in cases with BIS value between 56 and 65. Hypotension was observed in four cases, and bradycardia in six. Respiratory distress was observed in two cases with chronic pulmonary obstructive disease. All patients with adverse events were able to leave the hospital without extension of the hospitalization. CONCLUSION: BIS monitoring is useful to safely perform ESD. A BIS value of 70 to 75 is suitable for ESD.

关键词：内窥镜检查法镇静剂肿瘤化疗临床

Budd-Chiari syndrome: A case with a combination of hepatic vein and superior vena cava occlusion

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World Journal of Gastroenterology 2005年第24期11卷 3797-3799页

作者： Yoshio Araki Chikara Sakaguchi Izumi Ishizuka Masaya Sasaki Tomoyuki Tsujikawa Shigeki Koyama Akira Furukawa Yoshihide Fujiyama Department of Internal Medicine Department of Radiology

We here report a recent, rare case of Budd-Chiari syndrome, associated with a combination of hepatic vein and superior vena cava occlusion. A young female, who had been ingood health, was admitted to our hospital because of massive ascites. The patient had used no oral contraceptives. Tests for coagulation disorders, hematological disorders, and antiphospholipid syndrome were all negative. BuddChiari syndrome was diagnosed by radiographic examination. The patient was suffering from a combination of hepatic vein and superior vena cava occlusion. In particular, the venous flow returned from the liver mainly through a right accessory hepatic vein, and stenosis was recognized at the orifice of this collateral vein into the vena cava.Subsequently, the patient underwent percutaneous balloon dilatation therapy for this stenosis. After this treatment, the massive ascites was gradually reduced, and she was discharged from our hospital. It has now been one year since discharge, and the patient has been doing well. If deteriorating liver function or intractable ascites occur again, a liver transplantation may be anticipated. This is the first case report of Budd-Chiari syndrome associated with a superior vena cava occlusion.

关键词：肝静脉血管闭塞病理机制临床表现并发症

Hepatocellular carcinoma occurring in a Crohn’s disease patient

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World Journal of Gastroenterology 2010年第25期16卷 3215-3218页

作者： Mitsuaki Ishida Shigeyuki Naka Hisanori Shiomi Tomoyuki Tsujikawa Akira Andoh Tamio Nakahara Yasuharu Saito Yoshi-hide Fujiyama Mikiko Takikita-Suzuki Fumiyoshi Kojima Machiko Hotta Tohru Tani Yoshimasa Kurumi Hidetoshi Okabe Department of Clinical Lab-oratory Medicine and Division of Diagnostic Pathology Shiga University of Medical Science Otsu Shiga 520-2192 Japan Department of Surgery Shiga University of Medical Sci-ence Otsu Shiga 520-2192 Japan Department of Gastroenterology Shiga Uni-versity of Medical Science Otsu Shiga 520-2192 Japan Division of Endoscopy Shiga University of Medical Science Otsu Shiga 520-2192 Japan

We report a case of hepatocellular carcinoma (HCC) occurring in a patient with Crohn’s disease (CD) without chronic hepatitis or liver cirrhosis, and review the clinicopathological features of HCC in CD patients. A 37-year-old Japanese man with an 8-year history of CD and a medication history of azathioprine underwent resection of a liver tumor. The histopathology of the liver tumor was pseudoglandular type HCC. In the nonneoplastic liver, focal hepatocyte glycogenosis (FHG) was observed, however, there was no evidence of liver cirrhosis or primary sclerosing cholangitis. Only nine cases of HCC in CD patients have been reported previously in the English-language literature. Eight of 10cases (including the present case) had received azathioprine treatment, and four of these cases also showed FHG, which is considered a preneoplastic liver lesion, within the non-neoplastic liver. Although the precise mechanism of the development of HCC in CD patients is controversial, these results suggest that azathioprine therapy and FHG in the non-neoplastic liver contribute to the development of HCC. These findings also indicate that it is important to survey CD patients treated with prolonged azathioprine therapy for potential liver tumors.

关键词： Crohn’s disease Hepatocellular carcinoma Azathioprine Focal hepatocyte glycogenosis Hepato-carcinogenesis

Hepatic reactive lymphoid hyperplasia in a patient with primary biliary cirrhosis

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World Journal of Hepatology 2010年第10期2卷 387-391页

作者： Mitsuaki Ishida Tamio Nakahara Yousuke Mochizuki Tomoyuki Tsujikawa Akira Andoh Yasuharu Saito Hiroshi Yamamoto Fumiyoshi Kojima Machiko Hotta Tohru Tani Yoshihide Fujiyama Hidetoshi Okabe Department of Clinical Laboratory Medicine and Division of Diagnostic Pathology Shiga University of Medical Sci enceOtsuShiga 520-2192Japan Department of Gastroenterology Shiga University of Medical ScienceOtsuShiga 520-2192Japan Division of Endoscopy Shiga University of Medical ScienceOtsuShiga 520-2192Japan Department of Surgery Shiga University of Medical ScienceOtsuShiga 520-2192Japan

Reactive lymphoid hyperplasia(RLH) of the liver is an extremely rare lesion characterized by the proliferation of non-neoplastic lymphocytes forming follicles.Hepatic RLH is known to be associated with gastrointestinal carc inoma and autoimmune diseases including primary biliary cirrhosis(PBC).We report a case of hepatic RLH in a patient with PBC and gastric cancer.A 68 year old Japanese woman with a 10 year history of liver enzyme abnormality was admitted.Laboratory testing revealed that her anti-mitochondrial antibody was markedly elev ated.Five mo after the diagnosis of PBC,she was foun d to have gastric cancer.Abdominal computed tom og raphy disclosed a liver nodule in S8,suggesting metas tatic gastric carcinoma.Histopathologically,the resected liver lesion comprised of a nodular proliferation of small lym phocytes with lymphoid follicles.This is the first reported case of hepatic RLH in a patient with both PBC and gastric cancer.Pre-operative diagnosis of hepatic RLH by clinical imaging is extremely difficult.Therefore,a need le biopsy could be useful to make a diagnosis of hepat ic RLH,especially to differentiate from metastatic gastroin t estinal carcinoma.

关键词： Reactive lymphoid hyperplasia Pseudolymphoma Liver Primary biliary cirrhosis Gastric cancer

Non-Epstein-Barr virus associated lymphoepithelioma-like carcinoma of the inferior common bile duct

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World Journal of Gastrointestinal Oncology 2011年第7期3卷 111-115页

作者： Mitsuaki Ishida Tsuyoshi Mori Hisanori Shiomi Shigeyuki Naka Tomoyuki Tsujikawa Akira Andoh Yasuharu Saito Yoshimasa Kurumi Fumiyoshi Kojima Machiko Hotta Tohru Tani Yoshihide Fujiyama Hidetoshi Okabe Department of Clinical Laboratory Medicine and Division of Diagnostic Pathology Shiga University of Medical Science Department of Surgery Shiga University of Medical Science Department of Gastroenterology Shiga University of Medical Science Division of Endoscopy Shiga University of Medical Science

A carcinoma displaying undifferentiated features with dense lymphoplasmacytic infiltration is defined as a lymphoepithelioma-like carcinoma(LEC),and some of LEC is associated with Epstein-Barr virus(EBV).All of the 13 previously reported cases of LEC of the biliary system were intrahepatic in location.Herein,we describe the first case of LEC of the inferior common bile duct.A 68-year-old Japanese man,who had been previously treated for hepatocellular carcinoma using microwave coagulation therapy,was found to have tumors of the common bile duct and pancreas head.Histopathological study of the resected tumor showed solid or cohesive nests of large undifferentiated cells with irregular large vesicular nuclei and nucleoli.Around the tumor cell nests,dense lymphoplasmacytic infiltration was observed.Focal glandular differentiation(approximately 5%) was also present.These histopathological features corresponded morphologically to LEC.Immunohistochemically,the tumor cells were positive for cytokeratin(CK) 7,CK 19 and CA19-9,but negative for CK 20 and Hep Par 1.In situ hybridization for Epstein Barr virus early small RNAs disclosed no nuclear signal in tumor cells.Therefore,a diagnosis of non-EBV-associated LEC of the inferior common bile duct was made.Although the prognosis of the biliary LEC is thought to be better than that of conventional cholangiocarcinoma,the differences in prognosis between EBV-positive and-negative cases have not yet been established.Therefore,additional case studies will be needed to clarify the clinicopathological features of LEC of the biliary tract.

关键词： Lymphoepithelioma-like carcinoma EpsteinBarr virus Common bile duct

Usefulness of biodegradable stents constructed of poly-l-lactic acid monofilaments in patients with benign esophageal stenosis

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World Journal of Gastroenterology 2007年第29期13卷 3977-3980页

作者： Yasuharu Saito Toyohiko Tanaka Akira Andoh Hideki Minematsu Kazunori Hata Tomoyuki Tsujikawa Norihisa Nitta Kiyoshi Murata Yoshihide Fujiyama Department of Endoscopic Medicine Shiga University of Medical Science Seta Tukinowa Otsu 520-2192 Japan Department of Radiology Shiga University of Medical Science Seta Tukinowa Otsu 520-2192 Japan Department of Internal Medicine Shiga University of Medical Science Seta Tukinowa Otsu 520-2192 Japan

AIM: To report 13 patients with benign esophageal stenosis treated with the biodegradable stent. METHODS: We developed a Ultraflex-type stent by knitting poly-l-lactic acid monofilaments. RESULTS: Two cases were esophageal stenosis caused by drinking of caustic liquid, 4 cases were due to surgical resection of esophageal cancers, and 7 cases were patients with esophageal cancer who received the preventive placement of biodegradable stents for post- endoscopic mucosal dissection (ESD) stenosis. The preventive placement was performed within 2 to 3 d after ESD. In 10 of the 13 cases, spontaneous migration of the stents occurred between 10 to 21 d after placement. In these cases, the migrated stents were excreted with the feces, and no obstructive complications were experienced. In 3 cases, the stents remained at the proper location on d 21 after placement. No symptoms of re-stenosis were observed within the follow-up period of 7 mo to 2 years. Further treatment with balloon dilatation or replacement of the biodegradable stent was not required. CONCLUSION: Biodegradable stents were useful for the treatment of benign esophageal stenosis, particularly for the prevention of post-ESD stenosis.

关键词：良性食管狭窄食道癌内窥镜检查生物可降解聚乳酸