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第八次全国口腔颌面—头颈肿瘤会议

作者：刘少华魏奉才王勇郭斌宋晓彬 tomoki sumida Tomohide Yoshimura Hiroyuki Hamakawa 山东大学齐鲁医院口腔外科日本爱媛大学医学部口腔颌面外科

目的:Id(DNA结合抑制蛋白)是碱性螺旋-环-螺旋转录因子的负调节物,具有刺激细胞增生和抑制细胞分化的作用。近年来,在人体多种类型的肿瘤细胞系和肿瘤组织中检测到Id呈现高表达,但Id在头颈肿瘤中的作用尚不清楚。本研究旨在探讨Id分子...

目的:Id(DNA结合抑制蛋白)是碱性螺旋-环-螺旋转录因子的负调节物,具有刺激细胞增生和抑制细胞分化的作用。近年来,在人体多种类型的肿瘤细胞系和肿瘤组织中检测到Id呈现高表达,但Id在头颈肿瘤中的作用尚不清楚。本研究旨在探讨Id分子在头颈癌细胞恶性表型中的作用及其分子机制。方法:首先对多种头颈癌细胞系中Id1、Id2的蛋白表达进行了检测,然后对头颈癌细胞系进行了靶向Id分子的反意核苷酸转染或者RNA干扰,并同时检测了细胞增值活性、p21和c-myc表达变化以及细胞的侵袭性能。结果:与鳞状细胞癌(SCC)相比,源于涎腺肿瘤(SGC)的细胞系具有更高水平的Id1和Id2表达。在SCC细胞系中,分化程度越差者Id1的表达强度越高,然而Id2的表达未发现明显的规律。通过靶向Id分子的反意核苷酸稳定转染或者RNA干扰,头颈癌的增殖能力显著下降,并且这种增殖活性的降低伴随着p21的上调和c-myc的下调.细胞侵袭实验显示,经RNA干扰后,细胞丧失了浸润能力。实验结果提示,Id分子对头颈细胞的增殖和浸润起着重要的调节作用。结论:Id有可能成为抑制头颈癌进展的新的、有效的作用靶点。

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Serum autoantibody to sideroflexin 3 as a novel tumor marker for oral squamous cell carcinoma

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中国口腔颌面外科杂志 2008年第B5期6卷 105-105页

作者： Ryuichi Murase Yasuhito Abe tomoki sumida Koichi Nakashiro Hiroyuki Hamakawa Department of Oral and Maxillofacial Surgery Ehime University School of Medicine 454 Shitsukawa Toon city 7910295 Japan Department of Molecular Pathology Ehime University School of Medicine 454 Shitsukawa Toon city 7910295 Japan

Purpose: The purpose of this study was to establish a tumor marker that can be used for the early detection and follow-up of oral cancer patients。Methods: By employing the proteomic approach using MALDI TOF-MS, 2-D gel electrophoresis, sera of patients, and cell culture lines, the serum autoantibodies (autoAbs) were screened, and their serum levels were estimated by ELISA。Results: MRC-5 human fibroblasts were employed as target cells with the aim of targeting the tumor cell invasion into the surrounding stromal tissues, and a mitochondrial membrane protein, Sideroflexin3 (SFXN3), was identified。The serum anti-SFXN3-autoAb levels significantly increased in patients with oral squamous cell carcinoma, with 77% sensitivity and 89% specificity against control samples。The serum anti-SFXN3-autoAb levels slightly correlated with the primary tumor sizes; however, the levels were highly increased in TI early cancer。An immunohistochemical analysis revealed that the SFXN3 antigen is expressed in the stromal fibroblasts between the caner nests and also in the basal layer of the squamous epithelium。The changes in the serum anti-SFXN3-autoAb levels after therapy correlated with the clinical tumor burden。Conelusion: The serum anti-SFXN3-autoAb is worthy of clinical evaluation as a potential novel tumor marker for the early detection of oral squamous cell carcinoma。

关键词：口腔鳞状细胞癌血清自身抗性治疗方法

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Adverse effect on proliferation of human salivary gland tumor cells by estrogen and progesterone after introduction of their receptor

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中国口腔颌面外科杂志 2008年第B5期6卷 142-142页

作者： tomoki sumida Tomohide Yoshimura Akiko Onishi Koh-ichi Nakashiro Hiroyuki Hamakawa Department of Oral and Maxillofacial Surgery Ehime UniversitySchool of Medicine Ehime Japan

Purpose: Morphologic mimicry and similarity in the expression of steroid hormone receptors between salivary gland tumors (SGTs) and breast tumors are well-known phenomena and are occasionally debated in the field of surgical pathology。Estrogen (ES) and Progesterone (PG), the female sex steroid hormone, are intimately involved in breast cancer progression。However, little is known regarding its role in SGTs。Therefore, purpose of this study is to clarify the effect of sex steroid hormone on proliferation in SGT cells。Methods: In this study, we used ACCM, a human adenoid cystic carcinoma cell line established from the salivary gland, in order to clarify the role of their receptor on cell proliferation。Results: No effect of ES and PG on cell proliferation was observed in the estrogen receptor (ER) and progesterone receptor (PR)-deficient aggressive ACCM cells。However, after introducing ER and PR into the ACCM cells, ES accelerated, and PG markedly inhibited the proliferative activity of the cells。This adverse effect on cell proliferation was accompanied by the change of the expression levels of p21, Idl and c-myc。Conclusion: Our results provide significant insights into the mechanism of the proliferative property of the cells via the function of sex steroid hormone receptor。It is also suggested that the antagonist or blocker of ER, and PR reintroduction therapy might be a viable method of inhibiting human SGT progression。

关键词：唾液腺肿瘤细胞临床分析治疗方法

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One -step nucleic acid amplification for intraoperative detection of lymph node metastasis in oral cancer

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中国口腔颌面外科杂志 2008年第B5期6卷 103-103页

作者： Hiroyuki Goda Koh-ichi Nakashiro Tomohide Yoshimura Hidetomo Nishikawa tomoki sumida Hiroyuki Hamakawa Department of Oral and Maxillofacial Surgery Organ FunctionRegenerative and Reconstructive Surgery Ehime UniversityGraduate School of Medicine Ehime 791-0295 Japan

Purpose:To investigate the intraoperative molecular diagnosis of neck metastasis in oral cancer。Nodal status of the cervical lymph nodes remains an important prognostic factor。Management of the NO neck in oral cancer is an important issue for the oral surgeons。Sentinel lymph node biopsy has received considerable attention for its role in deciding whether to perform neck dissection。We performed the sentinel lymph node biopsy for clinical NO cases routinely and reported the clinical usefulness。The sentinel lymph node biopsy was followed by three methods to diagnose metastasis。The first method was hematoxylin-eosin staining。Lymph nodes were usually diagnosed as metastatic or not by examining one or two sections of the maximal cut-surface。The second method was semiserial sectioning, sliced at 200-p m intervals。The third method was genetic diagnosis using reverse transcriptase-polymerase chain reaction (RT-PCR)。However, the last method taken about three hours to get diagnosis。The aim of this study was to develop a more accurate and quick molecular detection system than the histological and RT-PCR methods。We examined an intraoperative diagnosis by One Step Nucleic acid Amplification (OSNA method) measuring cytokeratine 19 (CK19) mRNA and squamous cell carcinoma antigen (SCCA) mRNA。Methods: A cut-off value of CK19 mRNA copy number was introduced, using a criteria defined for breast cancer to distinguish macrometastasis, micrometastasis and isolated tumor cell。Two hundred and ten lymph nodes resected on sentinel lymph node biopsy and neck dissection were diagnosed by histological examination including semiserial sectioning。Alternative halves of these lymph nodes were diagnosed by calculating CK19 mRNA copy number using OSNA method。Further, we examined the LAMP method targeting SCCA mRNA。

关键词：口腔癌手术治疗治疗方法核酸

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