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Current profile and outcome of 100 esophageal atresia patients in the Kyushu area of Japan

Open Journal of Pediatrics 2013年第3期3卷 239-242页

作者： Nagata Kouji Esumi Genshirou Teshiba Risa Kinoshita Yoshiaki Taguchi tomoaki Department of Pediatric Surgery Reproductive and Developmental Medicine Graduate School of Medical Sciences Kyushu University Fukuoka Japan

Objectives: Since Spitz et al. reported the prognostic classification of esophageal atresia (EA) patients in 1994, decades have been past and there have been many advances in surgery and neonatology. Nevertheless, there have been very few reports according to the recent outcome of the neonates with EA, and otherwise, time has come to re-evaluate the credibility of this classification. The aim of this study was to validate the recent prognosis of the EA. Methods: Patient data were collected from 22 cooperative facilities during the 5 year period from 2005 to 2009 in Kyushu area, Japan. Total of 100 EA patients were retrospectively reviewed according to their characteristics and the outcome. Patient who missed the characteristics and outcome was excluded from the respective data. Results: Only 29.8% (28/94) was prenatally diagnosed and 52.0 (52/100) had associated anomalies including major congenital heart disease (CHD), abnormal chromosome, and others. According to the operation, primary anastomosis was performed 57.0% (57/100) and the staged operation was performed 34.0% (34/100). Survival rate in the neonatal period was 89.0% (89/ 100), and overall survival rate was 78.0% (78/100). According to the Spitz classification, if patients with associated anomalies were excluded, survival rate of Group 1 (>1500 g and no CHD) was 93.8% (61/65), Group 2 (<1500 g or CHD) was 68.4% (13/19), and Group 3 (<1500 g and CHD) was 50% (1/2). Conclusion: EA was proved to be rarely diagnosed prenatally. Primary outcome of the Group 1 and Group 3 in Spitz classification were fairly good, but Group 2 was worse as ever. The comprehensive treatment strategy for EA patients with birth weight under 1500 g or CHD should be reconsidered to improve the overall outcome.

关键词： Esophageal Atresia Outcome Low-Birth-Weight Infant Congenital Heart Disease Operation

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EPH-EPHRIN in human gastrointestinal cancers

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World Journal of Gastrointestinal Oncology 2010年第12期2卷 421-428页

作者： Haruhiko Sugimura Hiroki Mori Masaru Tsuboi Kiyoko Nagura Hisaki Igarashi Ritsuko Nakamura Hiroko Natsume tomoaki Kahyo Kazuya Shinmura Hiroyuki Konno Yasushi Hamaya Shigeru Kanaoka Hideki Kataoka Department of Pathology I Hamamatsu University School of MedicineHamamatsu 431-3192Japan Department of Surgery II Hamamatsu Uni- versity School of MedicineHamamatsu431-3192Japan Department of Molecular Diagnostics Hamamatsu University School of MedicineHamamatsu 431-3192Japan Department of Medicine I Hamamatsu University School of MedicineHamamatsu431-3192Japan

Ever since its discovery two decades ago,the erythro- poietin-producing hepatoma (EPH)-EPHRIN system has been shown to play multifaceted roles in human gastroenterological cancer as well as ***,amplif ication and point mutations have been found in many human cancers and many investigators have shown correlations between these up-regulationsand tumor ***,the genes in this family are considered to be potential targets of cancer *** the other hand,the down-regulation of some members as a result of epigenetic changes has also been reported in some ***,the correlation between altered expressions and clinical prognosis seems to be inconclusive.A huge amount of protein-protein interaction studies on the EPH-EPHRIN system have provided a basic scheme for signal transductions,especially bi-directional signaling involving EPH-ERPHRIN molecules at the cell *** information also provides a manipulative strategy for harnessing the actions of these *** this review,we summarize the known alterations of EPH-EPHRIN genes in human tumors of the esophagus,stomach,colorectum,liver and pancreas and present the perspective that the EPH-EPHRIN system could be a potential target of cancer therapy.

关键词： Erythropoietin-producing hepatoma EPH-EPHRIN Gastric cancer Colorectal cancer Methylation Secreted form

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Effects of Abscisic Acid Treatment on Berry Coloration and Expression of Flavonoid Biosynthesis Genes in Grape

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American Journal of Plant Sciences 2016年第9期7卷 1325-1336页

作者： Ayako Katayama-Ikegami tomoaki Sakamoto Kana Shibuya Takane Katayama Mei Gao-Takai Faculty of Bioresources and Environmental Sciences Ishikawa Prefectural University Ishikawa Japan Graduate School of Biostudies Kyoto University Kyoto Japan

In order to enhance berry coloration of bright-red grape cultivars, the effects of abscisic acid (ABA) treatment on the quantity and composition of anthocyanins as well as the expression of genes related to flavonoid biosynthesis in the berry were examined. Exogenous ABA treatment increased anthocyanin content, especially petunidin- and malvidin-type anthocyanins. Quantitative real-time PCR analysis revealed that ABA treatment around véraison resulted in the upregulation of genes encoding enzymes responsible for both general flavonoid and anthocyanin biosynthesis. On the other hand, the gene expressions of enzymes involved in proanthocyanidin synthesis were drastically decreased at véraison and remained extremely low even with ABA treatment. Thus, increases in the total amount and composition ratios of petunidin- and malvidin-type anthocyanins were mainly caused by ABA-induced upregulation of uridine diphosphate glucose flavonoid glucosyl transferase, glutathione S-transferase 4, O-methyl transferase and flavonoid 3’, 5’ hydroxylase expression, resulting in the deep coloration of berry of skin.

关键词： Abscisic Acid (ABA) Anthocyanin Biosynthetic Enzyme Gene Expression Transcription Factor

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Streamlined human antibody generation and optimization by exploiting designed immunoglobulin loci in a B cell line

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Cellular & Molecular Immunology 2021年第6期18卷 1545-1561页

作者： Hidetaka Seo Hitomi Masuda Kenjiro Asagoshi tomoaki Uchiki Shigehisa Kawata Goh Sasaki Takashi Yabuki Shunsuke Miyai Naoki Takahashi Shu-ichi Hashimoto Atsushi Sawada Aki Takaiwa Chika Koyama Kanako Tamai Kohei Kurosawa Ke-Yi Lin Kunihiro Ohta Yukoh Nakazaki Department of Life Sciences Graduate School of Arts and SciencesThe University of TokyoTokyoJapan Research Laboratories Chiome Bioscience IncTokyoJapan

Monoclonal antibodies(mAbs)are widely utilized as therapeutic drugs for various diseases,such as cancer,autoimmune diseases,and infectious *** the avian-derived B cell line DT40,we previously developed an antibody display technology,namely,the ADLib system,which rapidly generates antigen-specific ***,we report the development of a human version of the ADLib system and showcase the streamlined generation and optimization of functional human *** libraries were first constructed by replacing endogenous immunoglobulin genes with designed human *** these libraries,clones producing full-length human IgGs against distinct antigens can be isolated,as exemplified by the selection of antagonistic *** advantage of avian biology,effective affinity maturation was achieved in a straightforward manner by seamless diversification of the parental clones into secondary libraries followed by single-cell sorting,quickly affording mAbs with improved affinities and ***,we demonstrate that the human ADLib system could serve as an integrative platform with unique diversity for rapid de novo generation and optimization of therapeutic or diagnostic antibody ***,our results suggest that libraries can be constructed by introducing exogenous genes into DT40 cells,indicating that the ADLib system has the potential to be applied for the rapid and effective directed evolution and optimization of proteins in various fields beyond biomedicine.

关键词： Immunoglobulin rearrangements homologous recombination antibody therapeutics antibody engineering drug discovery

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抓斗下降时流场和最优下降方式的数值计算

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长江科学院院报 2017年第12期34卷 7-11,16页

作者：龙瑞 tomoaki Nakamura Yonghwan Cho Norimi Mizutan Tsuyoshi Kanazawa 长江科学院河流研究所武汉430010 Nagoya University Nagoya 464-8603Japan TOYO Construction Co. Ltd.Hyogo663-8142Japan

疏浚作业时如何能有效减少水体浑浊,减轻污染一直是人们关注的问题。利用可以分析流体和固体间运动相互作用的数学模型,模拟了环保型抓斗下降过程中的流场情况,并讨论了不同下降方式对流场的影响,提出了能适度控制流场扰动的操作方法。... 详细信息

疏浚作业时如何能有效减少水体浑浊,减轻污染一直是人们关注的问题。利用可以分析流体和固体间运动相互作用的数学模型,模拟了环保型抓斗下降过程中的流场情况,并讨论了不同下降方式对流场的影响,提出了能适度控制流场扰动的操作方法。结果表明:抓斗在下降过程中减速能有效减少底面流速,达到抑制底面浑浊的效果,其中抓斗在距离底面3m处减速为初始速度的1/3能最有效减少水体扰动从而抑制底面浑浊。研究结果可为环保型疏浚施工提供参考。

关键词：疏浚作业抓斗下降方式流场扰动数值计算水体浑浊

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Expression of leukotriene B4 receptor 1 defines functionally distinct DCs that control allergic skin inflammation

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Cellular & Molecular Immunology 2021年第6期18卷 1437-1449页

作者： tomoaki Koga Fumiyuki Sasaki Kazuko Saeki Soken Tsuchiya Toshiaki Okuno Mai Ohba Takako Ichiki Satoshi Iwamoto Hirotsugu Uzawa Keiko Kitajima Chikara Meno Eri Nakamura Norihiro Tada Yoshinori Fukui Junichi Kikuta Masaru Ishii Yukihiko Sugimoto Mitsuyoshi Nakao Takehiko Yokomizo Department of Biochemistry Juntendo University Graduate School of MedicineTokyo113-8421Japan Department of Medical Cell Biology Institute of Molecular Embryology and GeneticsKumamoto UniversityKumamoto860-0811Japan Department of Cell Signaling Graduate School of Medical and Dental SciencesTokyo Medical and Dental UniversityTokyo113-8510Japan Department of Pharmaceutical Biochemistry Graduate School of Pharmaceutical SciencesKumamoto UniversityKumamoto862-0973Japan Department of Developmental Biology Graduate School of Medical SciencesKyushu UniversityFukuoka812-8582Japan Laboratory of Genome Research Research Institute for Diseases of Old AgeJuntendo University Graduate School of MedicineTokyo113-8421Japan Division of Immunogenetics Department of Immunobiology and NeuroscienceMedical Institute of BioregulationKyushu UniversityFukuoka812-8582Japan Department of Immunology and Cell Biology Graduate School of Medicine and Frontier BiosciencesOsaka UniversityOsaka565-0871Japan

Leukotriene B4(LTB4)receptor 1(BLT1)is a chemotactic G protein-coupled receptor expressed by leukocytes,such as granulocytes,macrophages,and activated T *** there is growing evidence that BLT1 plays crucial roles in immune responses,its role in dendritic cells remains largely ***,we identified novel DC subsets defined by the expression of BLT1,namely,BLT1hi and BLT1lo *** also found that BLT1hi and BLT1lo DCs differentially migrated toward LTB4 and CCL21,a lymph node-homing chemoattractant,*** generating LTB4-producing enzyme LTA4H knockout mice and CD11c promoter-driven Cre recombinase-expressing BLT1 conditional knockout(BLT1 cKO)mice,we showed that the migration of BLT1hi DCs exacerbated allergic contact *** transcriptome analysis revealed that BLT1hi DCs preferentially induced Th1 differentiation by upregulating IL-12p35 expression,whereas BLT1lo DCs accelerated T cell proliferation by producing ***,the data reveal an unexpected role for BLT1 as a novel DC subset marker and provide novel insights into the role of the LTB4-BLT1 axis in the spatiotemporal regulation of distinct DC subsets.

关键词： LTB4 BLT1 dendritic cells inflammation lipid mediator

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Estimation of Three-Dimensional Volumetric Errors of Numerically Controlled Machine Tools by a Tracking Interferometer

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Journal of Mechanics Engineering and Automation 2011年第4期1卷 313-319页

作者： Soichi Ibaraki Kunitaka Takeuchi tomoaki Yano Toshiyuki Takatsuji Sonko Osawa Osamu Sato Department of Micro Engineering Graduate School of Engineering Kyoto University Yoshida-honmachi Sakyo-ku Kyoto606-8501 Japan Fine Manufacturing Systems Group Advanced Manufacturing Research Institute National Institute of Advanced IndustrialScience and Technology Japan National Metrology Institute of Japan National Institute of Advanced Industrial Science and Technology Japan

This paper presents the estimation of three-dimensional volumetric errors of a machining center by using a tracking interferometer. A tracking interferometer is a laser interferometer with the mechanism to steer the laser direction to follow a target retroreflector. Based on the triangulation principle, the three-dimensional position of the target can be estimated from measured laser displacements. Its capability to measure three-dimensional positioning errors for arbitrary trajectories is important for the indirect measurement of the machine＇s kinematic model. This paper presents experimental investigation of the estimation accuracy of the multilateration-based measurement by a tracking interferometer. A tracking interferometer developed by a part of the authors is used in experiments. In the present experiment, the measured volume of target positions was 100 mm × 100 mm × 100 mm. The estimation accuracy of targets within this volume was not sufficiently high compared to the positioning error of the measured machine tool. The results of the experiment and simulation show that the estimation uncertainty is dependent on tracking interferometer locations relative to target locations. Error sensitivity analysis shows that wider distribution of tracker positions in XY improves the estimation accuracy.

关键词： Tracking interferometer laser tracker volumetric errors multilateration machine tools estimation accuracy.

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Enhanced hepatic differentiation in the subpopulation of human amniotic stem cells under 3D multicellular microenvironment

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World Journal of Stem Cells 2019年第9期11卷 705-721页

作者： Kinji Furuya Yun-Wen Zheng Daisuke Sako Kenichi Iwasaki Dong-Xu Zheng Jian-Yun Ge Li-Ping Liu tomoaki Furuta Kazunori Akimoto Hiroya Yagi Hiromi Hamada Hiroko Isoda Tatsuya Oda Nobuhiro Ohkohchi Department of Gastrointestinal and Hepato-Biliary-Pancreatic Surgery Faculty of MedicineUniversity of TsukubaTsukubaIbaraki 305-8575Japan Institute of Regenerative Medicine and Affiliated Hospital Jiangsu UniversityZhenjiang 212001Jiangsu ProvinceChina Department of Regenerative Medicine School of MedicineYokohama City UniversityYokohama 236-0004Japan Department of Medicinal and Life Sciences Faculty of Pharmaceutical SciencesTokyo University of ScienceNoda 278-8510Japan Department of Obstetrics and Gynecology Faculty of MedicineUniversity of TsukubaTsukuba 305-8575Japan Faculty of Life and Environmental Sciences University of TsukubaTsukuba 305-8572Japan

BACKGROUND To solve the problem of liver transplantation donor insufficiency,an alternative cell transplantation therapy was *** focused on amniotic epithelial cells(AECs)as a cell source because,unlike induced pluripotent stem cells,they are cost-effective and *** utilization of AECs in regenerative medicine,however,is in its infancy.A general profile for AECs has not been comprehensively ***,no hepatic differentiation protocol for AECs has yet been *** this end,we independently compiled human AEC libraries,purified amniotic stem cells(ASCs),and co-cultured them with mesenchymal stem cells(MSCs)and human umbilical vein endothelial cell(HUVECs)in a 3D system which induces functional hepatic *** To characterize AECs and generate functional hepatic organoids from ASCs and other somatic stem cells METHODS AECs,MSCs,and HUVECs were isolated from the placentae and umbilical cords of cesarean section *** and primary AEC stemness characteristics and heterogeneity were analyzed by immunocytochemistry,Alkaline phosphatase(AP)staining,and flow *** adherent AEC subpopulation was selected and evaluated for ASC purification quality by a colony formation *** transcriptomes were compared with those for other hepatocytes cell sources by *** 2D and 3D culture were compared by relative gene expression using several differentiation ***,MSCs,and HUVECs were combined in a 3D co-culture system to generate hepatic organoids whose structure was compared with a 3D AEC sphere and whose function was elucidated by immunofluorescence imaging,periodic acid Schiff,and an indocyanine green(ICG)*** AECs have certain stemness markers such as EPCAM,SSEA4,and *** AEC subpopulation was also either positive for AP staining or expressed the TRA-1-60 and TRA-1-81 stemness ***,it could form colonies and its frequency was enhanced ten-fold in the adherent subpo

关键词： 3D micropattern Amniotic epithelial cells Amniotic stem cells Hepatic differentiation Heterogeneity Human placental tissue Human umbilical vein endothelial cells Mesenchymal stem cells Multicellular microenvironment Organoid

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Inverse relationship between platelet Akt activity and hippocampal atrophy:A pilot case-control study in patients with diabetes mellitus

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World Journal of Clinical Cases 2024年第2期12卷 302-313页

作者： Haruhiko Tokuda Takamitsu Hori Daisuke Mizutani Tomoyuki Hioki Kumi Kojima Takashi Onuma Yukiko Enomoto tomoaki Doi Rie Matsushima-Nishiwaki Shinji Ogura Hiroki Iida Toru Iwama Takashi Sakurai Osamu Kozawa Department of Metabolic Research Research InstituteNational Center for Geriatrics and GerontologyObu 474-8511Japan Department of Clinical Laboratory National Center for Geriatrics and GerontologyObu 474-8511Japan Department of Pharmacology Gifu University Graduate School of MedicineGifu 501-1194Japan Department of Neurosurgery Gifu University Graduate School of MedicineGifu 501-1194Japan Department of Dermatology Central Japan International Medical CenterMinokamo 505-8510Japan Department of Anesthesiology and Pain Medicine Gifu University Graduate School of MedicineGifu 501-1194Japan Department of Emergency Medicine Faculty of MedicineUniversity of ToyamaToyama 930-0194Japan Department of Emergency and Disaster Medicine Gifu University Graduate School of MedicineGifu 501-1194Japan Research Institute National Center for Geriatrics and GerontologyObu 474-8511Japan

BACKGROUND Akt plays diverse roles in *** is involved in the pathogenesis of type 2 diabetes mellitus(T2DM),which is caused by insulin *** also plays a vital role in human platelet ***,the hippocampus is closely associated with memory and learning,and a decrease in hippocampal volume is reportedly associated with an insulin-resistant phenotype in T2DM patients without *** To investigate the relationship between Akt phosphorylation in unstimulated platelets and the hippocampal volume in T2DM *** Platelet-rich plasma(PRP)was prepared from the venous blood of patients with T2DM or age-matched *** pellet lysate of the centrifuged PRP was subjected to western blotting to analyse the phosphorylation of Akt,p38 mitogen-activated protein(MAP)kinase and glyceraldehyde 3-phosphate dehydrogenase(GAPDH).Phosphorylation levels were quantified by densitometric *** volume was analysed using a voxel-based specific regional analysis system for Alzheimer’s disease on magnetic resonance imaging,which proposes the Z-score as a parameter that reflects hippocampal *** The levels of phosphorylated Akt corrected with phosphorylated p38 MAP kinase were inversely correlated with the Z-scores in the T2DM subjects,whereas the levels of phosphorylated Akt corrected with GAPDH were ***,this relationship was not observed in the control *** These results suggest that an inverse relationship may exist between platelet Akt activation and hippocampal atrophy in T2DM *** findings provide insight into the molecular mechanisms underlying T2DM hippocampal atrophy.

关键词： Akt Platelet Hippocampal atrophy Magnetic resonance imaging Diabetes mellitus

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Numerical Simulation of Flow Behavior in Basilar Bifurcation Aneurysms Based on 4-Dimensional Computed Tomography Angiography

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World Journal of Mechanics 2021年第4期11卷 71-82页

作者： tomoaki Yamazaki Gaku Tanaka Ryuhei Yamaguchi Yodai Okazaki Hitomi Anzai Fujimaro Ishida Makoto Ohta Graduate School of Engineering Chiba University Chiba Japan Institute of Fluid Science Tohoku University Sendai Japan School of Engineering Tokyo Institute of Technology Yokohama Japan Department of Neurosurgery Mie Chuo Medical Centre Tsu Japan

Initiation, growth, and rupture of cerebral aneurysms are caused by hemodynamic factors. It is extensively accepted that the cerebral aneurysm wall is assumed to be rigid using computational fluid dynamics (CFD). Furthermore, fluid-structure interactions have been recently applied for simulation of an elastic cerebral aneurysm model. Herein, we examined cerebral aneurysm hemodynamics in a realistic moving boundary deformation model based on 4-dimensional computed tomographic angiography (4D-CTA) obtained by high time-resolution using numerical simulation. The aneurysm of the realistic moving deformation model based on 4D-CTA at each phase was constructed. The effect of small wall deformation on hemodynamic characteristics might be interested. So, four hemodynamic factors (wall shear stress, wall shear stress divergence, oscillatory shear index and residual residence time) were determined from the numerical simulation, and their behaviors were assessed in the basilar bifurcation aneurysm.

关键词： Basilar Bifurcation Aneurysm 4-Dimensional Computed Tomographic Angiography Moving Boundary Method

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