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Design of 16S rRNA gene primers for 454 pyrosequencing of the human foregut microbiome

World Journal of Gastroenterology 2010年第33期16卷 4135-4144页

作者： Carlos W Nossa William E Oberdorf Jφrn A Aas Bruce J Paster todd z desantis Eoin L Brodie Daniel Malamud Michael A Poles zhiheng Pei Department of Medicine New York University School of Medicine Department of Molecular Genetics The Forsyth Institute Faculty of Dentistry University of Oslo Harvard School of Dental Medicine Lawrence Berkeley National Laboratory Center for Environmental Biotechnology New York University College of Dentistry Depar tment of Pa thology and Medicine New York University School of MedicineNew YorkNY 10016United States Department of Veterans Affairs New York Harbor Health SystemNewYorkNY 10010United States

AIM:To design and validate broad-range 16S rRNA primers for use in high throughput sequencing to classify bacteria isolated from the human foregut ***:A foregut microbiome dataset was constructed using 16S rRNA gene sequences obtained from oral,esophageal,and gastric microbiomes produced by Sanger sequencing in previous studies represented by 219 bacterial *** primers evaluated were from the European rRNA *** assess the effect of sequence length on accuracy of classification,16S rRNA genes of various lengths were created by trimming the full length *** spanning various hypervariable regions were selected to simulate the amplicons that would be obtained using possible primer *** sequences were compared with full length 16S rRNA genes for accuracy in taxonomic classification using online software at the Ribosomal Database Project (RDP).The universality of the primer set was evaluated using the RDP 16S rRNA database which is comprised of 433 306 16S rRNA genes,represented by 36 ***:Truncation to 100 nucleotides(nt)downstream from the position corresponding to base 28 in the Escherichia coli 16S rRNA gene caused misclassification of 87(39.7%)of the 219 sequences,compared with misclassification of only 29(13.2%)sequences with truncation to 350 *** 350-nt sequence reads within various regions of the 16S rRNA gene,the reverse read of an amplicon generated using the 343F/798R primers had the least(8.2%)effect on *** comparison,truncation to 900 nt mimicking single pass Sanger reads misclassified 5.0%of the 219 *** 343F/798R amplicon accurately assigned 91.8%of the 219 sequences at the species *** by abundance of the species in the esophageal dataset,the 343F/798R amplicon yielded similar classification accuracy without a significant loss in species coverage(92%).Modification of the 343F/798R primers to 347F/803R increased their universality among foregut ***

关键词： Foregut Microbiome 16S 454 sequencing Primer

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Carbon pool trends and dynamics within a subtropical peatland during long-term restoration

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Ecological Processes 2017年第1期6卷 449-462页

作者： Paul Julian II Stefan Gerber Alan L.Wright Binhe Gu todd z.Osborne University of Florida Soil and Water Sciences DepartmentFt.PierceFL 34945USA. University of Florida Soil and Water Sciences DepartmentGainesvilleFL 32611USA. South Florida Water Management District Water Quality BureauWest Palm BeachFL 33406USA. University of Florida Whitney Laboratory for Marine BioscienceSt AugustineFL 32080USA.

Introduction:The Florida Everglades has undergone significant ecological change spanning the continuum of disturbance to *** the restoration effort is not complete and the ecosystem continues to experience short duration perturbations,a better understanding of long-term C dynamics of the Everglades is needed to facilitate new restoration *** study evaluated temporal trends of different aquatic carbon(C)pools of the northern Everglades Protection Area over a 20-year period to gauge historic C cycling *** inorganic C(DIC),dissolved organic C(DOC),particulate organic C(POC),and surface water carbon dioxide(pCO2(aq))were investigated between May 1,1994 and April 30,***:Annual mean concentrations of DIC,DOC,POC,and pCO2(aq)significantly decreased through time or remained constant across the Water Conservation Areas(WCAs).Overall,the magnitude of the different C pools in the water column significantly differed between *** of CO2 was dynamic across the Everglades ranging from 420 to 2001 kg CO2 year−***,the historic trend in CO2 flux from the marsh declined across our study area while pCO2(aq)largely remained somewhat constant with the exception of Water Conservation Area 2 which experienced significant declines in pCO2(aq).Particulate OC concentrations were consistent between WCAs,but a significantly decreasing trend in annual POC concentrations was ***:Hydrologic condition and nutrient inputs significantly influenced the balance,speciation,and flux of C pools across WCAs suggesting a subsidy-stress response in C dynamics relative to landscape scale responses in nutrient *** interplay between nutrient inputs and hydrologic condition exert a driving force on the balance between DIC and DOC production via the metabolism of organic matter which forms the base of the aquatic food *** the restoration trajectory as water quality and hydrology continues to improve,it is expect

关键词： Inorganic carbon Organic carbon Carbon dioxide Particulate carbon Hydrology Nutrients

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The acceleration of a high-charge electron bunch to 10 GeV in a 10-cm nanoparticle-assisted wakefield accelerator

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Matter and Radiation at Extremes 2024年第1期9卷 15-24页

作者： Constantin Aniculaesei Thanh Ha Samuel Yoffe Lance Labun Stephen Milton Edward McCary Michael M.Spinks Hernan J.Quevedo Ou z.Labun Ritwik Sain Andrea Hannasch Rafal zgadzaj Isabella Pagano Jose A.Franco-Altamirano Martin L.Ringuette Erhart Gaul Scott V.Luedtke Ganesh Tiwari Bernhard Ersfeld Enrico Brunetti Hartmut Ruhl todd Ditmire Sandra Bruce Michael E.Donovan Michael C.Downer Dino A.Jaroszynski Bjorn Manuel Hegelich University of Texas at Austin AustinTexas 78712USA SUPA Department of Physics University of StrathclydeGlasgowScotland G4 ONGUnited Kingdom Tau Systems Inc.AustinTexas 78701USA Lawrence Livermore National Laboratory LivermoreCalifornia 94550USA Los Alamos National Laboratory Los AlamosNew Mexico 87545USA Brookhaven National Laboratory UptonNew York 11973USA Ludwig-Maximilians-Universität MunichGermany

An intense laser pulse focused onto a plasma can excite nonlinear plasma *** appropriate conditions,electrons from the background plasma are trapped in the plasma wave and accelerated to ultra-relativistic *** scheme is called a laser wakefield *** this work,we present results from a laser wakefield acceleration experiment using a petawatt-class laser to excite the wakefields as well as nanoparticles to assist the injection of electrons into the accelerating phase of the *** find that a 10-cm-long,nanoparticle-assisted laser wakefield accelerator can generate 340 pC,10±1.86 GeV electron bunches with a 3.4 GeV rms convolved energy spread and a 0.9 mrad rms *** can also produce bunches with lower energies in the 4–6 GeV range.

关键词： acceleration nanoparticle charge

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Evaluation of nutrient stoichiometric relationships among ecosystem compartments of a subtropical treatment wetland. Do we have “Redfield wetlands”?

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Ecological Processes 2019年第1期8卷 267-287页

作者： Paul Julian II Stefan Gerber Rupesh K.Bhomia Jill King todd z.Osborne Alan L.Wright Matthew Powers Jacob Dombrowski Soil and Water Sciences Department University of FloridaFt.PierceFL34945USA Soil and Water Sciences Department University of FloridaGainesvilleFL 32611USA South Florida Water Management District Water Quality Treatment TechnologiesWest Palm BeachFL 33406USA Whitney Laboratory for Marine Bioscience University of FloridaSt AugustineFL 32080USA.

Background:Evaluation of carbon(C),nitrogen(N),and phosphorus(P)ratios in aquatic and terrestrial ecosystems can advance our understanding of biological processes,nutrient cycling,and the fate of organic matter(OM)in these *** of aquatic ecosystems can change the accumulation and decomposition of OM which can alter biogeochemical cycling and alter the base of the aquatic food *** study investigated nutrient stoichiometry within and among wetland ecosystem compartments(i.e.,water column,flocculent,soil,and aboveground vegetation biomass)of two subtropical treatment wetlands with distinct vegetation *** flow-ways(FWs)within the network of Everglades Stormwater Treatment Areas in south Florida(USA)were selected for this *** evaluated nutrient stoichiometry of these to understand biogeochemical cycling and controls of nutrient removal in a treatment wetland within an ecological stoichiometry ***:This study demonstrates that C,N,and P stoichiometry can be highly variable among ecosystem compartments and between *** law slopes of C,N,and P within surface water floc,soil,and vegetation were significantly different between and along ***:Assessment of wetland nutrient stoichiometry between and within ecosystem compartments suggests unconstrained stoichiometry related to P that conforms with the notion of P limitation in the *** in N:P ratios between floc and soil suggest different pathways of organic nutrient accumulation and retention between *** nutrient stoichiometry was highly variable and decoupled(or close to decoupled as indicated byze that decoupling may be the imprint of variability in inflow nutrient ***,despite active biogeochemical cycles that could act to restore nutrient stoichiometry along the FW,there was little evidence that such balancing occurred,as the

关键词： Decomposition Mineralization Everglades Treatment wetlands

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Recent developments in the detection of melamine

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Journal of zhejiang University-Science B(Biomedicine & Biotechnology) 2012年第7期13卷 525-532页

作者： Yuan LIU Ewen E.D.todd Qiang zHANG Jiang-rong SHI Xian-jin LIU Key Laboratoy.of Food Quality and Safety of Jiangsu Province Nanjing 210014China Michigan State Universities East Lansing 48824-1212USA

In recent years,there were two reported outbreaks of food borne illness associated with *** presence of melamine and its related compounds in milk,feed,and other foods has resulted in the need for reliable methods for the detection and accurate quantification of this class of *** sample pretreatment for melamine in a complex matrix usually involves a liquid extraction by a polar solvent,followed by a further clean-up with solid phase *** of melamine and related compounds are commonly carried out by liquid or gas chromatographic methods conjugated with mass *** innovative screening methods,which use antibodies,molecularly imprinted polymers,capillary electrophoresis,and gold nanoparticles,are also used to develop assays and biosensors to ***,many of these methods have been hindered by matrix effects,the solubility of melamine-cyanuric acid complex,and background *** article reviews recent developments for detecting melamine and discusses future directions.

关键词： Melamine Detection Confirmation methods Screening methods Sample pretreatment

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A detailed study of metallic glass formation in copper-hafnium-titanium alloys

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Acta Metallurgica Sinica(English Letters) 2012年第6期25卷 409-419页

作者： Ignacio A.Figueroa Sandro Baez-Pimiento John D.Plummer Omar Novelo-Peralta Hywell A.Davies Iain todd Department of Metallic and Ceramic Materials Institute for Materials ResearchNational Autonomous University of Mexico(UNAM)Mexico D.F.C.P.04510Mexico Department of Materials Imperial College LondonRoyal School of MinesPrince Consort RoadSW7 2BPU.K. Department of Materials Science and Engineering University of SheffieldMappin StSheffield S1 3JDU.K.

Recently we have reported a number of bulk glass forming compositions in the CuHf-Ti system,with the critical thickness for complete glass formation,dc,ranging from 2 mm to 4 *** order to improve the glass forming ability （GFA） getting even larger dc,the prevailing approach is to use complex multicomponent *** strategy has been investigated by us for the Cu-Hf-Ti bulk glass forming alloy Cu55Hf25Ti20 using 〉1 *** additions of B,Y,Nb,Ta,Al,Mn,Si or V but with no significant improvement in the ***,it is necessary,in order to utilise the full potential of the base ternary system,to identify the best glass-forming compositions as a basis for extending the search into multi-dimensional compositional ***,CuxHfyTiz alloys,where x=（40-70） ***,y=（5-30） ***,and z=（10-36） ***,were prepared by melt spinning andcopper mould *** composition dependence of the GFA for the Cu-Hf-Ti alloys,as measured by dc for rod and ribbon samples,is reported over the composition range given above.

关键词： Metallic glass formation Glassy Cu-Hf-Ti alloy Glass formingability Melt spinning Copper mould suction-casting

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应用认知隐喻学的特征与趋势--《在真实世界中研究和应用隐喻》评介

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外语研究 2012年第5期29卷 107-108页

作者：吕晶晶 Low.Graham z.todd A.Deignan L.Cameron 华东师范大学上海200241 上海外国语大学上海200083

Researching and Applying Metaphor in the Real World, Low, Graham, z. todd, A. Deignan ~ L. Cameron, Amsterdam：John Benjams Publishing Company,2010.1．前言概念隐喻理论自提出以来，并未得到一致的反响和回应，尤其在近十年，遭遇了较多的质疑和挑战。“真实世界隐喻”的研究者们即试图通过基于语言背景的真实世界隐喻研究来进行回应，

关键词：认知隐喻世界 Metaphor 应用评介概念隐喻理论 World Real

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Protein-based profiling of the human IgA1 clonal repertoire revealed shared clones of serum polymeric IgA1 and milk secretory IgA1

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Cellular & Molecular Immunology 2023年第3期20卷 305-307页

作者： Jan Novak Matthew B.Renfrow R.Glenn King Colin Reily todd J.Green Department of Microbiology University of Alabama at BirminghamBirminghamALUSA Department of Biochemistry and Molecular Genetics University of Alabama at BirminghamBirminghamALUSA

Human adaptive immunity involves the production and secretion of antibodies by differentiated B cells(ie.,antibody-secreting cells,also called plasma cells)in response to *** secreted antigen-specific antibodies are immunoglobulins that are present at substantial concentrations in blood,mucosal secretions,and breast ***,human immunoglobulins consist of two identical heavy(H)chains and two identical light(L)chains that are connected by disulfide bridges to form a homodimer of heterodimers(ie.,H+Ll2).Each heterodimer consists of one H chain linked with one L chain,whereas the homodimer is formed by linking two H chains *** genes for both the L and H chains are encoded by ligated gene segments that are genetically rearranged during V(D)J recombination,a process that endows each B cell with a unique receptor,giving rise to the enormous diversity of the B-cell antigen receptor repertoire.

关键词： IgA1 immunity chains

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Supplier Selection and Management

Supplier Selection and Management

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中国质协2001年学术年会暨第二届中美质量管理交流研讨会

作者： todd

z-I质量委员会和美国Technical AdvisoryGroup(USTAG)到这国际质量标准技术委员会组织Organization for StandardizationTechnical Committee On Quality (ISOTC176)的一个会员.todd先生是USSubcommittee 1(SC 1)两任的质量术语主席。

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LiF@SiO2 nanocapsules for controlled lithium release and osteoarthritis treatment

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Nano Research 2018年第10期11卷 5751-5760页

作者： Trever todd zhenhui Lu Jinmin zhao Benjamin Cline Weizhong zhang Hongmin Chen Anil Kumar Wen Jiang Franklin West Samuel Franklin Li zheng Jin Xie Department of Chemistry University of Georgia Athens Georgia 30602 USA Guangxi Engineering Center in Biomedical Material for Tissue and Organ Regeneration Guangxi Collaborative Innovation Center for Biomedicine Guangxi Key Laboratory of Regenerative Medicine The First Affiliated Hospital of Guangxi Medical University Nanning 530021 China Center for Molecular Imaging and Translational Medicine State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics School of Public Health Xiamen University Xiamen 361102 China Animal Dairy Science University of Georgia Athens Georgia 30602 USA Department of Small Animal Medicine and Surgery College of Veterinary Medicine University of Georgia Athens Georgia 30602 USA

Electrolytes can be taken orally or intravenously as supplements or thera- peutics. However, their therapeutic window may exceed the serum toxicity threshold, making systemic delivery a poor option. Local injection is also not adequate due to rapid diffusion of electrolytes. Here, we solved this issue with a nanocapsule technolog~ comprising an electrolyte nanocrystal as the drug filling and a silica sheath to regulate drug release rates. In particular, we prepared LiF@SiO2 nanocapsules and investigated their potential as a delivery system for lithium, which was shown in recent studies to be an effective therapeutic agent for osteoarthritis （OA）. We demonstrated that LiF@SiO2 can extend lithium release time from minutes to more than 60 h. After intra- articular （i.a.） injection into a rat OA model, the nanocapsules reduced the Osteoarthritis Research Society International （OARSI） score by 71% in 8 weeks while inducing no systemic toxicity. Our study opens new doors for improved delivery of electrolyte therapeutics, which have rarely been studied in the past.

关键词： osteoarthrifis controlled release hyaluronic acid lithium anti-inflammation

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