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Analysis of trace.dicyandiamide.in stre.m wate. using solid phase.e.traction and liquid chromatography UV spe.trome.ry

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Journal of e.vironme.tal Scie.ce. 2015年第9期27卷 38-42页

作者： Huidong Qiu Dongdi Sun Same.ra R.Gunatilake.Jinyan she todd e.Mlsna De.artme.t of Che.istry and Che.ical e.gine.ring Chongqing University of Science and Technology De.artme.t of Che.istry Mississippi State University

An improve. me.hod for trace.le.e. quantification of dicyandiamide.in stre.m wate. has be.n de.e.ope.. This me.hod include. sample.pre.re.tme.t using solid phase.*** e.traction proce.ure.including loading, washing, and e.uting） use. a flow rate.of1.0 m L/min, and dicyandiamide.was e.ute. with 20 m L of a me.hanol/ace.onitrile.mixture.V/V = 2：3）, followe. by pre.conce.tration using nitroge. e.aporation and analysis with high pe.formance.liquid chromatography–ultraviole. spe.troscopy（HPLC–UV）. Sample.e.traction was carrie. out using a Wate.s Se.-Pak AC-2 Cartridge.with activate. carbon）.Se.aration was achie.e. on a ZIC-Hydrophilic Inte.action Liquid Chromatography（ZIC-HILIC）（50 mm × 2.1 mm, 3.5 μm） chromatography column and quantification was accomplished base. on UV absorbance. A re.iable.line.r re.ationship was obtaine. for the.calibration curve.using standard solutions（R^2〉 0.999）. Re.ove.ie. for dicyandiamide.range. from 84.6% to 96.8%, and the.re.ative.standard de.iations（RSDs, n = 3） we.e.be.ow 6.1% with a de.e.tion limit of 5.0 ng/m L for stre.m wate. sample..

关键词： Dicyandiamide.Solid phase.e.traction Stre.m wate. sample. High pe.formance.liquid chromatography–ultraviole. spe.trome.ry

Ge.e.al synthe.is of silica-base. yolk/shell hybrid nanomate.ials and in vivo tumor vasculature.targe.ing

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Nano Re.e.rch 2018年第9期11卷 4890-4904页

作者： Fe.g Che. Shre.a Goe. Sixiang Shi todd e. Barnhart Xiaoli Lan We.bo Cai De.artme.t of Radiology University of Wisconsin-Madison W153705 USA Mate.ials Scie.ce.Program University of Wisconsin-Madison W153705 USA De.artme.t of Me.ical Physics University of Wisconsin-Madison WI 53705 USA De.artme.t of Nucle.r Me.icine Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 China Unive.sity of Wisconsin Carbone.Cance. Ce.te. Madison W153705 USA

Multifunctional yolk/shell-structure. hybrid nanomate.ials have.attracte. incre.sing inte.e.t as the.anostic nanoplatforms for cance. imaging and the.apy. Howe.e., be.ause.of the.lack of suitable.surface.e.gine.ring and tumor targe.ing strate.ie., pre.ious re.e.rch has focuse. mainly on nanostructure.de.ign and synthe.is with fe. succe.sful e.ample. showing active.tumor targe.ing afte. syste.ic administration. In this study, we.re.ort the.ge.e.al synthe.ic strate.y of che.ator-fre. zirconium-89 （89Zr）-radiolabe.e., TRC105 antibody-conjugate., silica-base. yolk/sheU hybrid nanopartide. for in vivo tumor vasculature.targe.ing. Thre. type. of inorganic nanoparticle. with varying morphologie. and size. we.e.se.e.te. as the.inte.nal core., which we.e.e.capsulate. into single.hollow me.oporous silica nanosheUs to form the.yolk/sheU-structure. hybrid nanopartide.. As a proof-of-conce.t, we.de.onstrate. succe.sful surface.functionalization of the.nanoparticle. with polye.hyle.e.glycol, TRC105 antibody （spe.ific for CD105/e.doglin）, and ~Zr （a positron-e.itting radioisotope., and e.hance. in vivo tumor vasculature.targe.e. positron e.ission tomography imaging in 4T1 murine.bre.st tumor-be.ring mice. This strate.y could be.applie. to the.synthe.is of othe. type. of yolk/shell the.anostic nanoparticle. for tumor- targe.e. imaging and drug de.ive.y.

关键词： yolk/shelLintrinsic radiolabe.ing vasculature.targe.ing positron e.ission tomography zirconium-89

Optimizing me.hyl-e.ge.ol aromathe.apy to maximize.posttre.tme.t e.fe.ts to e.hance.mating compe.itive.e.s of male.Bactroce.a carambolae.（Dipte.a： Te.hritidae.

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Inse.t Scie.ce 2015年第5期22卷 661-669页

作者： Ihsan ul Haq Marc J.B. Vre.se. Carlos Cace.e. todd e. she.ly Jorge.He.drichs Inse.t Pe.t Control Laboratory Joint FAOIIAEA Division of Nuclear Techniques in Food and Agriculture Seibersdorf Austria National Agricultural Re.e.rch Ce.tre Park Road Islamabad Pakistan USDAIAPHIS 41-650 Ahiki Street Waimanalo HI 96795 USA and Inse.t Pe.t Control Se.tion Joint FAOIIAEA Division of Nuclear Techniques in Food and Agriculture Vienna Austria

Me.hyl-e.ge.ol （Me. （1,2-dime.hoxy-4-（2-prope.yl）be.ze.e., a natural phy- toche.ical, did e.hance.male.Bactroce.a carambolae.Dre. ＆ Hancock （Dipte.a： Te.hri- tidae. mating compe.itive.e.s 3 d afte. inge.tion. e.hance. male.mating compe.itive.e.s can significantly incre.se.the.e.fe.tive.e.s of the.ste.ile.inse.t te.hnique.（SIT）. Me.appli- cation to mass re.re. ste.ile.flie. by fe.ding is infe.sible. Me.application by aromathe.apy howe.e., would be.a ve.y practical way of Me.application in fly e.e.ge.ce.and re.e.se.facilitie.. This approach was shown to e.hance.mating compe.itive.e.s ofB. carambolae.3 d posttre.tme.t （DPT）. De.pite.this adde. be.e.it, e.e.y additional day of de.aying re. le.se.will re.uce.ste.ile.fly quality and will add cost to SIT application. The.pre.e.t study was planne. to asse.s the.e.fe.ts of Me.aromathe.apy on male.B. carambolae.mating compe.itive.e.s 1DPT and 2DPT. Me.aromathe.apy 1DPT or 2DPT did e.hance.mating compe.itive.e.s of B. carambolae.male. whe.e.s Me.fe.ding 1DPT and 2DPT did not. Male.mating compe.itive.e.s was e.hance. by the.Me.aromathe.apy irre.pe.tive.if the. re.e.ve. 1DPT, 2DPT or 3DPT. Me.aromathe.apy, be.ng a viable.approach for its applica- tion, did e.hance.mating compe.itive.e.s of male.B. carambolae.1 d posttre.trne.t as Me.fe.ding did 3 d afte. inge.tion.

关键词： Bactroce.a carambolae mating compe.itive.e.s Me.aromathe.apy me.hyl-e.ge.ol Me.fe.ding posttre.tme.t e.fe.t SIT

e.pre.sion of an e.doglucanase.from Tribolium castane.m （Tce. 1 ） in Saccharomyce. ce.e.isiae

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Inse.t Scie.ce 2014年第5期21卷 609-618页

作者： De.e. Shirle. Cris Oppe.t todd B. Re.nolds Be.hany Miracle.Bre.da Oppe.t William e. Klinge.an Juan Luis Jurat-Fue.te. De.artme.t of e.tomology and Plant Pathology University of Tennessee De.artme.t of Microbiology University of TennesseeKnoxville TN 37996 USDA-Agricultural Re.e.rch Se.vice.Ce.te. for Grain and Animal He.lth Re.e.rch Manhattan KS 66502 De.artme.t of Plant Scie.ce. University of Tennessee Knoxville TN 37996 USA

Inse.ts are.a large.y une.ploite. re.ource.in prospe.ting for nove. ce.lulolytic e.zyme. to improve.the.production of e.hanol fue. from lignoce.lulosic biomass. The.cost of lignoce.lulosic e.hanol production is e.pe.te. to de.re.se.by the.combination of ce.lulose.de.radation （saccharification） and fe.me.tation of the.re.ulting glucose.to e.hanol in a single.proce.s, catalyze. by the.ye.st Saccharomyce. ce.e.isiae.transforme. to e.pre.s e.ficie.t ce.lulase.. While.S. ce.e.isiae.is an e.tablished he.e.ologous e.pre.sion syste., the.e.are.no available.data on the.functional e.pre.sion of inse.t ce.lulolytic e.zyme. for this spe.ie.. To addre.s this knowle.ge.gap, S. ce.e.isiae.was transforme. to e.pre.s the.full-le.gth cDNA e.coding an e.doglucanase.from the.re. flour be.tle. Tribolium castane.m （Tce. 1）, and e.aluate. the.activity of the.transge.ic product （rTce. 1）. e.pre.sion of the.Tce.1 cDNA in S. ce.e.isiae.was unde. control of the.strong glyce.alde.yde.3 phosphate.de.ydroge.ase.promote.. Culture. transforme. ye.st se.re.e. rTce.1 prote.n as a functional β-1,4-e.doglucanase. which allowe. transformants to survive.on se.e.tive.me.ia containing ce.lulose.as the.only available.carbon source. e.aluation of substrate.spe.ificity for se.re.e. rTce.1 de.onstrate. e.doglucanase.activity, although some.activity was also de.e.te. against comple. ce.lulose.substrate.. Pote.tially re.e.ant to use. in biofue. production rTce.1 activity incre.se. with pH conditions, with the.highe.t activity de.e.te. at pH 12. Our re.ults de.onstrate.the.pote.tial for functional production of an inse.t ce.lulase.in S. ce.e.isiae.and confirm the.stability of rTce.1 activity in strong alkaline.e.vironme.ts.

关键词： biofue.s inse.t ce.lulase Saccharomyce. ce.e.isiae Tribolium castane.m ye.st

Succe.sful e.doscopic tre.tme.t of colonic gallstone.ile.s using e.e.trohydraulic lithotripsy

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World Journal of Gastroe.te.ology 2010年第12期16卷 1533-1536页

作者： Martin D Zie.inski Lincoln e.Fe.re.ra todd H Baron Division of Trauma Critical Care and General Surgery De.artme.t of Me.icine Federal University of Juiz de Fora De.artme.t of Gastroe.te.ology and He.atology

The.surgical manage.e.t of gallstone.ile.s is comple. and pote.tially highly *** manage.e.t re.uire. e.te.olithotomy and is ge.e.ally followe. by fistula re.e.tion at a late. *** have.be.n re.orts of gallstone.e.traction using various e.doscopic modalitie. to re.ie.e.the.obstruction,howe.e.,to date.the.e.has ne.e. be.n a published case.of e.doscopic stone.e.traction from the.colon using e.e.trohydraulic *** this re.ort,we.pre.e.t the.te.hnique.e.ploye. to succe.sfully pe.form an e.e.trohydraulic lithotripsy for re.oval of a large.gallstone.impacte. in the.sigmoid colon.A cavity was e.cavate. in an obstructing 4.1 cm lame.late. stone.in the.sigmoid colon using e.e.trohydraulic lithotripsy.A scre. ste.t re.ractor and ste.t e.tractor bore. a large. lume. which allowe. for guide.ire.advance.e.t and stone.fracture.via se.ial pne.matic balloon *** stone.fragme.ts we.e.*** lithotripsy is a safe.and e.fe.tive.me.hod to tre.t colonic obstruction in the.se.ting of gallstone.ile.s.

关键词： Gallstone.ile.s e.doscopy e.e.trohydraulic lithotripsy Bowe. obstruction Colon

Circulating microRNA e.pre.sion and nonalcoholic fatty live. dise.se.in adole.ce.ts with se.e.e.obe.ity

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World Journal of Gastroe.te.ology 2024年第4期30卷 332-345页

作者： Yi-Jie.Li Brittne. O Baume.t Nikos Stratakis Je.se.A Goodrich Hao-Tian Wu Jing-Xuan He.Yin-Qi Zhao Max T Aung Hong-Xu Wang Sandrah P e.ke. Douglas I Walke. Damaskini Valvi Miche.e.A La Me.rill Justin R Ryde. Thomas H Inge.todd Je.kins Ste.hanie.Sisle. Rohit Kohli Stavra A Xanthakos Andre. A Baccare.li Rob McConne.l David V Conti Lida Chatzi De.artme.t of Population and Public He.lth Scie.ce. Keck School of MedicineUniversity of Southern CaliforniaLos AngelesCA 90032United States Barce.ona Institute.of Global He.lth Barcelona Institute of Global HealthBarcelona 08036Spain De.artme.t of e.vironme.tal He.lth Scie.ce. Mailman School of Public HealthColumbia UniversityNew YorkNY 10032United States Gangarosa De.artme.t of e.vironme.tal He.lth Rollins School of Public HealthEmory UniversityAtlantaGA 30329United States De.artme.t of e.vironme.tal Me.icine.and Public He.lth Icahn School of Medicine at Mount SinaiNew YorkNY 10029United States De.artme.t of e.vironme.tal Toxicology University of CaliforniaDavisCA 95616United States De.artme.t of Surge.y Lurie Children’s Hospital of ChicagoChicagoIL 60611United States Northwe.te.n Unive.sity Fe.nbe.g School of Me.icine Northwestern UniversityChicagoIL 60611United States Division of Biostatistics and e.ide.iology Cincinnati Children's Hospital Medical CenterCincinnatiOH 45229United States De.artme.t of Pe.iatrics University of Cincinnati College of MedicineCincinnatiOH 45229United States De.artme.t of Pe.iatrics Children’s Nutrition Research Center USDA/ARSBaylor College of MedicineHoustonTX 77030United States De.artme.t of Gastroe.te.ology Children’s Hospital Los AngelesLos AngelesCA 90027United States Division of Gastroe.te.ology Hepatology and NutritionCincinnati Children's Hospital Medical CenterCincinnatiOH 45229United States

BACKGROUND Nonalcoholic fatty live. dise.se.NAFLD)is one.of the.most common chronic live. dise.se. in childre. and *** range. in se.e.ity from isolate. he.atic ste.tosis to nonalcoholic ste.tohe.atitis(NASH),whe.e.n he.atoce.lular inflammation and/or fibrosis coe.ist with *** microRNA(miRNA)le.e.s have.be.n sugge.te. to be.alte.e. in NAFLD,but the.e.te.t to which miRNA are.re.ate. to NAFLD fe.ture. re.ains *** analysis te.te. the.hypothe.is that plasma miRNAs are.significantly associate. with histological fe.ture. of NAFLD in *** To inve.tigate.the.re.ationship be.we.n plasma miRNA e.pre.sion and NAFLD fe.ture. among adole.ce.ts with *** This study include. 81 adole.ce.ts diagnose. with NAFLD and 54 adole.ce.ts without NAFLD from the.Te.n-Longitudinal Asse.sme.t of Bariatric Surge.y ***-ope.ative.core.live. biopsie. we.e.colle.te. from participants and use. to characte.ize.histological fe.ture. of *** sample. we.e.colle.te. during surge.y for miRNA profiling.A total of 843 plasma miRNAs we.e.profile. using the.HTG e.ge.e. *** e.amine. associations of plasma miRNAs and NAFLD fe.ture. using logistic re.re.sion afte. adjusting for age.se.,race.and othe. ke. *** Pathways Analysis was use. to ide.tify biological functions of miRNAs that we.e.associate. with multiple.histological fe.ture. of *** We.ide.tifie. 16 upre.ulate. plasma miRNAs,including miR-193a-5p and miR-193b-5p,and 22 downre.ulate. plasma miRNAs,including miR-1282 and miR-6734-5p,in adole.ce.ts with ***,52,16,15,and 9 plasma miRNAs we.e.associate. with NASH,fibrosis,ballooning de.e.e.ation,and lobular inflammation,***,16 miRNAs we.e.associate. with two or more.histological fe.ture. of *** those.miRNAs,miR-411-5p was downre.ulate. in NASH,ballooning,and fibrosis,while.miR-122-5p,miR-1343-5p,miR-193a-5p,miR-193b-5p,and miR-7845-5p we.e.consiste.tly and posi

关键词： MicroRNA Nonalcoholic fatty live. dise.se Non-alcoholic ste.tohe.atitis Live. fibrosis Lobular inflammation Ballooning de.e.e.ation

Se.sonal influe.ce.and local factors affe.ting macroinve.te.rate.structure.in a high-altitude.Ande.n stre.m

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Journal of Mountain Scie.ce 2020年第6期17卷 1374-1386页

作者： SCHe.BLe. e.ica e.Fe.NáNDe. CAMPóN Flore.cia LAGOS SILNIK Susana We.LNITZ todd Laboratorio de.e.tomologia IADIZACCT CONICET MendozaAvenida Ruiz Leal s/nParque Gral.San MartinCC 5075500MendozaArgentina Conse.o Nacional de.Inve.tigacione. Cie.tificas y Te.nicas(CONICe.) Avenida Rivadavia 1917C1033AAJCABAArgentina Facultad de.Cie.cias e.actas y Naturale. Universidad Nacional de CuyoPadre Jorge Contreras 1300M5502JMA MendozaArgentina Biology De.artme.t University o f Wisconsin-Eau Claire105 Garfield Avenue54701Eau ClaireWisconsinUSA

Small wate. bodie. are.critical for maintaining fre.hwate. biodive.sity,but are.among the.le.st inve.tigate. aquatic *** e.amine. physical and che.ical variable. at two re.che. in Arroyo Tambillos,a small,high-e.e.ation Ande.n stre.m,in NW Me.doza province.Arge.tina,across four se.sons to e.amine.how local factors and se.sonality affe.te. the.structure.of the.macroinve.te.rate.*** Arroyo Tambillos community was nume.ically dominate. by e.he.e.opte.ans(mainly Massarte.lopsis irarrazavali)while.the.dipte.an Chironomidae.we.e.the.most spe.ie. *** macroinve.te.rate.abundance.was highe.t in summe.,drive. mainly by taxa that we.e.se.ondary in dominance.Austre.mis *** Ande.iops pe.uvianus),while.richne.s did not diffe. be.we.n ***,be.thic composition was diffe.e.t in Fall 2013(March)compare. to othe. se.sons,large.y be.ause.of the.incre.se. abundance.of *** corre.ponde.ce.analysis discriminate. Chironomidae.spe.ie. distributions in Fall 2013 by substrate.type.i.e.,big and small boulde. substrate..By contrast,discharge.ve.ocity,and de.th we.e.the.variable. which most affe.te. the.macroinve.te.rate.abundance.and *** to our e.pe.tations,most community change. obse.ve. occurre. in fall inste.d of ***-kryal stre.m communitie. like.the.one.de.cribe. he.e.have.be.ome.incre.singly important for conse.ving mountain stre.m biodive.sity as anthropoge.tic impacts and climate.change.incre.singly impact lowe. stre.m ***,monitoring high-altitude.stre.ms like.the.Arroyo Tambillos may be.critical for pre.e.ting the.future.loss of unique.and se.sitive.stre.m biota.

关键词： e.he.e.opte.a dominance Chironomidae.richne.s Mountain small stre.m Physical variable. Biodive.sity e.osyste.

The.acce.e.ation of a high-charge.e.e.tron bunch to 10 Ge. in a 10-cm nanoparticle.assiste. wake.ie.d acce.e.ator

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Matte. and Radiation at e.tre.e. 2024年第1期9卷 15-24页

作者： Constantin Aniculae.e. Thanh Ha Samue. Yoffe.Lance.Labun Ste.he. Milton e.ward McCary Michae. M.Spinks He.nan J.Que.e.o Ou Z.Labun Ritwik Sain Andre. Hannasch Rafal Zgadzaj Isabe.la Pagano Jose.A.Franco-Altamirano Martin L.Ringue.te.e.hart Gaul Scott V.Lue.tke.Gane.h Tiwari Be.nhard e.sfe.d e.rico Brune.ti Hartmut Ruhl todd Ditmire.Sandra Bruce.Michae. e.Donovan Michae. C.Downe. Dino A.Jaroszynski Bjorn Manue. He.e.ich Unive.sity of Te.as at Austin AustinTexas 78712USA SUPA De.artme.t of Physics University of StrathclydeGlasgowScotland G4 ONGUnited Kingdom Tau Syste.s Inc.AustinTexas 78701USA Lawre.ce.Live.more.National Laboratory LivermoreCalifornia 94550USA Los Alamos National Laboratory Los AlamosNew Mexico 87545USA Brookhave. National Laboratory UptonNew York 11973USA Ludwig-Maximilians-Unive.sität MunichGermany

An inte.se.lase. pulse.focuse. onto a plasma can e.cite.nonline.r plasma *** appropriate.conditions,e.e.trons from the.background plasma are.trappe. in the.plasma wave.and acce.e.ate. to ultra-re.ativistic *** sche.e.is calle. a lase. wake.ie.d *** this work,we.pre.e.t re.ults from a lase. wake.ie.d acce.e.ation e.pe.ime.t using a pe.awatt-class lase. to e.cite.the.wake.ie.ds as we.l as nanoparticle. to assist the.inje.tion of e.e.trons into the.acce.e.ating phase.of the.*** find that a 10-cm-long,nanoparticle.assiste. lase. wake.ie.d acce.e.ator can ge.e.ate.340 pC,10±1.86 Ge. e.e.tron bunche. with a 3.4 Ge. rms convolve. e.e.gy spre.d and a 0.9 mrad rms *** can also produce.bunche. with lowe. e.e.gie. in the.4–6 Ge. range.

关键词： acce.e.ation nanoparticle charge

Pink1 and parkinde.onstrate.multiface.e. role. whe. co-e.pre.se. with Foxo

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Advance. in Parkinson's Dise.se 2013年第1期2卷 5-10页

作者： Amy M. todd Brian e. Stave.e. De.artme.t of Biology Memorial University of Newfoundland St. John’s Canada

Pink1 has be.n linke. to both autosomal re.e.sive.and sporadic forms of Parkinson dise.se. The.Pink1 prote.n is thought to be.involve. in mitochondrial prote.tion by inte.acting with parkin to pre.e.t oxidative.damage. maintain mitochondrial inte.rity and re.ulate.mitophagy. Pink1 and parkin have.be.n linke. to compone.ts of the.insulin re.e.tor (INR) pathway, including PTe., Akt and Foxo, but the.r e.fe.ts in the.INR pathway have.be.n large.y ove.looke.. To furthe. inve.tigate.the.role. of Pink1/parkin, we.have.pe.forme. co-e.pre.sion studie. to de.e.mine.the.e.fe.ts Pink1 and parkin on the.Foxo-induce. phe.otype.of de.e.opme.tal de.e.ts in the.Drosophila e.e. We.e.amine. dire.te. e.pre.sion of Pink1, parkin, Pink1 or parkin mutants, and Pink1 or parkin inte.fe.ing RNAs (RNAi) with the.ove.e.pre.sion of Foxo in the.de.e.oping e.e.of Drosophila. Our findings show that re.uction of Pink1 suppre.se. the.e.fe.ts of Foxo ove.e.pre.sion, whe.e.co-ove.e.pre.sion with Pink1 or parkin incre.se. the.se.e.ity of the.phe.otype. This sugge.ts that Pink1 and parkin are.able.to incre.se.the.pro-apoptotic e.fe.ts of Foxo. Contrary to the.vie. that Pink1 and parkin act e.clusive.y as prote.tive.prote.ns in the.ce.l, it is like.y that the.Pink1/parkin pathway is involve. in aspe.ts of ce.l fate.de.isions othe. than de.rading toxic prote.ns and maintaining mitochondrial inte.rity.

关键词： Pink1 Parkin Foxo Drosophila